Tiêu Chuẩn Iso 09308-1-2014 Amd1-2016.Pdf

© ISO 2016 Water quality — Enumeration of Escherichia coli and coliform bacteria — Part 1 Membrane filtration method for waters with low bacterial background flora AMENDMENT 1 Qualité de l’eau — Dénom[.]

Water quality — Enumeration

ofEsche richia coli and c oliform

Par t 1:

Qu lité de l’ea — Dé ombr ment de s Esche ichia col et de s

b c térie s coliforme s —

Partie 1: Méth de p r filtration sur membra e p ur les ea x à fible 

teneur en b ctéries

AMENDEMENT1

T ir d edition

2 14-0 -1

Reference n mb r

ISO 9 0 -1:2 14/A md.1:2 16(E)

AMENDMENT 1

2 16-1 -1

COPYRIGHT PROTECTED DOCUMENT

© ISO 2016, P blshed in Sw itz rlan

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Amen ment 1 t o ISO 9 0 -1:2 14 was pr p r d b Te hnical C mmitt ee ISO/TC 147, Wate q alt y,

Subcommitt ee SC4, Mic ro iolo ic al meth ds

Water quality — Enumeration ofEsche richia coli and

Part 1:

Pa e 2,Cla s e 4,s ec ond p ra ra h

R eplac the se on p ra ra h with the folowing

Incub tion ofthe membrane f ilt er at (3 ± 2) °Cfor (2 t o 24 ) h

Pa e 3, 8.3, firs t p ra ra h

R eplac the firs p ra ra h with the folowing

Aft er f iltration ( 8.2), plac the membrane filt er on the Chromog enic Col form Ag r (CCA) (B.1),

ensuring that no air is tra ped u de neath, inve t the Petri dish, an incub t e at (3 ± 2) °C for

(2 t o 24 ) h

Pa e 4,1 2

R eplac Ta le 1 with the folowing

Table 1 — Performance testing of Chromog enic Colform Ag r

Fu ction Incub tion C ntrol s rains R efe enc

medium

Meth d of

control

Crit eria

(Pro-d ctivity)

C aract eris ic

r actions

Prod ctivity (2 t o 24) h/

(36 ± 2) °C

E c ol

WDCM 0 0 3or

WDCM0 0 2

TSA Quantitative PR ≥ 0,7

Dark-blue t o

violet colonies

Ent a r g n s

WDCM 0 175 or

C fe ndi

WDCM 0 0 6

TSA Quantitative PR ≥ 0,7

Pin t o red

colonies

S lectivity (2 t o 24) h/

(36 ± 2) °C

E f ec ali s

WDCM0 0 7 or

WDCM 0 0 9

Totalor partial

in ibition

—

Specif icity (2 t o 24) h/

(36 ± 2) °C

P a rugin s a

WDCM0 0 4

Colourles

colonies

a

R efer to the referenc s rain c talog e avaiable (view ed 2016-10-28) on ht p:/www.wfc info /p f/WDCM_R eferenc _

St ain_Catalog e.p f on culture c le tion s rain n mber an c nta t detais