TEST BANK microbiology a systems approach cowan 3e chapter017

Direct antigen testing differs from direct fluorescence testing in that the results A.. The property of a te st to dete ct evensmall amount s of antibodies or antigen s th at ar e te st

Chapter 017 Diagnosing Infections

Multiple Choice Questions

1 Which of the following is not a phenotypic method of identification?

3 The primary advantage of genotypic methods of identification is

A It is easier than any other

7 Direct antigen testing differs from direct fluorescence testing in that the results

A Are read with the naked

8 Urinean

d fecalspecimen

s require

A

B

C

D

E

9 Phag

e typing

is usef

ul

in identifyi

10 Animalsar

e require

d forth

e cultivation

11 Whic

h te

st

is especially goodforbacteri

a th

at ar

e no

t readily cultivated

in th

e lab?

A

B

C

D

E

12 Whic

h

of th

e following

is no

t agenotypicmetho

d

of identification?

A

B

C

D

E

13 Whic

h te

st compare

s th

e percentage

of cytosin

e an

d guanin

e base

s

to adenin

e an

d thyminebases?

A

B

C

D

E

14 Whic

h

of th

e following

is involve

d

in th

e

in vit

ro diagnosti

c testing

of serum

E

15 The propert

y

of

a te

st

to dete

ct evensmall amount

s

of antibodies

or antigen

s th

at ar

e te

st targe

ts

is

A

B

C

D

E

16 The propert

y

of

a te

st

to dete

ct on

ly

a certa

in antibod

y

or antigenan

d no

t

to reac

t wit

h an

y othe

rs

is

A

B

C

D

E

17 A seru

m tit

er involve

s

A

B

C

D

E

18 A positiveserologica

l te

st fortuberculosi

s indicat

es th

at

A

B

C

D

19 Serologica

l testingca

n beused

to te

st each

of th

e followingexce

20 Titer

is th

e amou

21 Serologica

l testingreliesup

on

A

B

C

D

E

22 Antibod

y testingrequire

23 Wha

t type

of te

st wil

l dete

ct whol

e antigens?

24 Solubl

e antigen

s ar

e detected

in thi

s type

of te

25 Wha

t type

of te

st afxe

s anantigen

to aninertparticlesuch

as

a latexbead

?

A

B

C

D

E

26 Precipitationtestsinvolveall th

e followingexce

pt

A

B

C

D

E

27 Whe

n seru

m protei

ns ar

e separatedbyelectrophoresi

s an

d thenantibodiesspecific forth

e seru

m protei

ns ar

e placed

in

a parallel troug

h

in orde

r

to for

m reactio

n ar

cs foreachprotei

n, th

e te

st

is calle

d

A

B

C

D

E

28 Whic

h

of th

e following

is no

t anagglutinatio

n test?

A

B

C

D

E

29 In precipitationtests, th

e antig

E

30 Syphilisca

n bediagnose

d byth

E

31 The Wida

l te

st

is determina

nt for

32 The VRDLte

st detects

33 Whic

h

of th

e following

is incorrec

t regardingth

e complemen

t fixationtest?

A

B

C

D

E

34 Whic

h te

st

is th

e confirmin

g te

st forpeop

le wh

o initially testedantibody-positive

in th

e screeningELISAtest?

A

B

C

D

E

35 Serotypin

g

of th

e pneumococcu

s base

d oncapsul

ar polysaccharide

is performed

in

A

B

C

D

E

36 The Wester

n bl

ot te

st

is confirmator

y forHI

V becaus

e

A

B

C

D

E

37 Whic

h te

st

is used

to diagno

se polio

38 Whic

h te

st

is used

to confirmscarl

et fever?

39 Whic

h te

st

is used

to confirmrheumati

c fever?

40 The Quellun

g te

st involve

E

41 Whic

h te

st us

es radioactiveisotope

s

to labe

l antibodies

or antigen

s

in orde

r

to dete

ct minu

te amount

s

of correspondi

or antibody?

42 The dy

es fluorescei

n an

d rhodami

ne

A

B

C

D

E

43 Horseradishperoxida

se an

d alkalinephosphatas

e ar

e enzym

es used

in _ tests

A

B

C

D

E

44 A positiveELISAresul

t require

s

A

B

C

D

E

45 The indirec

t ELISAte

st

is used

to identif

y all

of th

e followingexce

E

46 The direc

t ELISAte

st

is used

to identif

y all

of th

e followingexce

E

47 A simpl

e metho

d

to dete

ct _ cel

ls involve

s mixingthe

m wit

h untreatedshee

p re

d bloo

d cel

ls th

at wil

l bindan

d

m rosette

48 In viv

o testing

is usef

ul fordiagnosin

49 The tuberculi

n ski

n te

st

is re

50 Whe

n minu

te sampl

es

of DNAneed

to begeneticall

y analyze

d foridentificatio

n purposes,whichte

st ca

n beeffectivelyused

?

51 It is necessar

y

to dola

b tests

to diagno

se all disease

s

Tr

ue False

52 Whe

n antibodies

or othe

r substanc

es

in seru

m cross-reac

t wit

h th

e te

st reagents,

a fal

se positiveresul

t ca

n occu

r

ue False53 Serologica

l testingalwa

ys involve

s reactio

ns betwee

n specific antibod

y an

d antigen

Tr

ue False

54 Serologica

l testsshou

ld havelo

w sensitivityan

d specifici

ty

Tr

ue False

55 The Weil-Fel

ix te

st

is usedfordiagnosin

g salmonellosi

s

Tr

ue False

56 ELISAtestsus

e

a fluoresce

nt dy

e

as

a labe

l

to tra

ce antigen-antibod

y reactio

ns

Tr

ue False

57 The tuberculi

n te

st

is anexampl

e

of an

in vit

ro serologica

l te

st

Tr

ue False

58 Airor flu

id sampl

es ca

n bemonitore

d throughbiosensorin

g

Tr

ue False

59 The polymeras

e chai

n reactio

n require

s endonucleas

e

to cu

t sampl

es

of DNA

Tr

ue False

Chapter 017 Diagnosing Infections Key

Multiple Choice Questions

1 Whic

h

of th

e following

is no

t aphenotyp

ic metho

d

of identification? A

B

C

D

E

Lea rnin g Obj ecti ve: 17 01 Na me the thre e maj or cat ego ries of mic rob e ide ntif cati on tec hni que s.

2 Biochemicaltestsincludeall

of th

e followingexce

pt A

B

C

D

Lea rnin g Obj ecti ve: 17 01 Na me the thre e maj or cat ego ries of mic rob e ide ntif cati on tec hni que s.

3 Th

e primar

y advantag

e

of genotypicmetho

ds

of identificatio

n

is A

B

D

E

Lea rnin g Obj ecti ve: 17 01 Na me the thre e maj or cat ego ries of mic rob e ide ntif cati on tec hni que s.

4 Specimencollectio

n A

B

C

E

Lea rnin g Obj ecti ve: 17 02 Ide ntif y som e imp orta nt con side rati ons abo ut coll ecti ng sam ples

fro m pati ent s for mic robi al ide ntif cati on.

5 Each

of th

e followingca

n beusedforspecimencollectio

n exce

pt A

B.C

D

E

Lea rnin g Obj ecti ve: 17 02 Ide ntif

y som e imp orta nt con side rati ons abo ut coll ecti ng sam ples fro m pati ent s for mic robi al ide ntif cati on.

6 Whic

h sampl

e

is no

t collecte

d bysteri

le need

le aspiration

? A

B

C

D

E

Lea rnin g Obj ecti ve: 17 02 Ide ntif y som e imp orta nt con side rati ons abo ut coll ecti ng sam ples fro m pati ent s for mic robi al ide ntif cati on.

7 Direc

t antigentestingdiffe

rs fro

m direc

t fluorescenc

e testing

in th

at th

e resul

ts

A

B

D

E

Lea rnin g Obj ecti ve: 17 04 List at leas

t 3

dif ere nt test s that fall in the dire ct ide ntif cati on cat ego ry.

8 Urinean

d fecalspecimen

s require

A

B

C

E

Lea rnin g Obj ecti ve: 17 02 Ide ntif y som e imp orta nt con side rati ons abo ut coll ecti ng sam ples fro m pati ent s for mic robi al ide ntif cati on.

9 Phag

e typing

is usef

ul

in identifyi

Lea rnin g Obj ecti ve: 17 03 Expl ain the idea s beh ind pre sum ptiv e vs con frm ator y dat a.

10 Animalsar

e require

d forth

e cultivation

Lea rnin g Obj ecti ve: 17 03 Expl ain the idea s beh ind pre sum ptiv e vs con frm ator y dat a.

11 Whic

h te

st

is especially goodforbacteri

a th

at ar

e no

t readily cultivated

in th

e lab? A

B

D

E

Lea rnin g Obj ecti ve: 17 04 List at leas

t 3 dif ere nt test s that fall in the dire ct ide

ntif cati on cat ego ry.

12 Whic

h

of th

e following

is no

t agenotypicmetho

d

of identification? A

B

C

D

Lea rnin g Obj ecti ve: 17 04 List at leas

t 3 dif ere nt test s that fall in the dire ct ide ntif cati on cat ego ry Lea rnin g Obj ecti ve: 17 06 List the maj or ste ps

in a hyb ridiz atio n

met hod of mic rob e ide ntif cati on.

13 Whic

h te

st compare

s th

e percentage

of cytosin

e an

d guanin

e base

s

to adenin

e an

d thyminebases? A

t 3 dif ere nt test s that fall in the dire ct

ide ntif cati on cat ego ry Lea rnin g Obj ecti ve: 17 06 List the maj or ste ps

in a hyb ridiz atio n met hod of mic rob e ide ntif cati on.

14 Whic

h

of th

e following

is involve

d

in th

e

in vit

ro diagnosti

c testing

of serum

? A

B

C

D

Lea rnin g Obj ecti ve: 17 05 Expl ain the mai n prin cipl e beh ind bioc he mic al test s Lea rnin g Obj ecti ve: 17 08 Giv

e a thor oug h def niti on of the ter m ser olog y.

15 The propert

y

of

a te

st

to dete

ct evensmall amount

s

of antibodies

or antigen

s th

at ar

e te

st targe

ts

is A

16 The propert

y

of

a te

st

to dete

ct on

ly

a certa

in antibod

y

or antigenan

d no

t

to reac

t wit

h an

y othe

rs

is A

17 A seru

m tit

er involve

s A

B

C

D

E

Lea rnin g Obj ecti ve: 17 10 Disc uss the con cep ts of agg luti nati on and pre cipit atio n and whe n eac

h is app ropr iate

18 A positiveserologica

l te

st fortuberculosi

s indicat

es th

at A

B

C

D

Lea rnin g

Obj ecti ve: 17 09 Dif ere ntia te bet wee n sen sitiv ity and spe cifc ity.

19 Serologica

l testingca

n beused

to te

st each

of th

e followingexce

pt A

B

C.D

E

Lea rnin g Obj ecti ve: 17 08 Giv

e a thor oug h def niti on of the ter m ser olog y Lea rnin g Obj ecti ve: 17 09 Dif ere ntia te bet wee n sen sitiv ity and spe cifc ity.

20 Titer

is th

e amou

nt

of A

B

C

D

E

Lea rnin g Obj ecti ve: 17 10 Disc uss the con cep ts of agg luti nati on and pre cipit atio n and whe n eac

h is app ropr iate

21 Serologica

l testingreliesup

on

A

B

D

E

Lea rnin g Obj ecti ve: 17 05 Expl ain the mai n prin cipl e beh ind bioc he mic al test s Lea rnin g Obj ecti ve: 17 08 Giv

e a thor oug h def niti on of the ter m ser olog y.

22 Antibod

y testingrequire

s A

B

C

D

Lea rnin g Obj ecti ve: 17 09 Dif ere ntia te bet wee n sen sitiv ity and spe cifc ity Lea rnin g Obj ecti ve: 17 10 Disc uss the con cep ts of agg luti nati on and pre cipit atio n and whe n eac

h is app ropr iate

23 Wha

t type

of te

st wil

l dete

ct whol

e antigens? A

Obj ecti ve: 17 10 Disc uss the con cep ts of agg luti nati on and pre cipit atio n and whe n eac

h is app ropr iate

24 Solubl

e antigen

s ar

e detected

in thi

s type

of te

Lea rnin g Obj ecti ve: 17 10 Disc uss the con cep ts of agg luti nati on and pre cipit atio n and whe n eac

h is app ropr iate

25 Wha

t type

of te

st afxe

s anantigen

to aninertparticlesuch

as

a latexbead

? A

B

C

E

Lea rnin g Obj ecti ve: 17 10 Disc uss the con cep ts of agg luti nati on and pre cipit atio n and whe n eac

h is app ropr iate

26 Precipitationtestsinvolveall th

e followingexce

pt

A

B

D

Lea rnin g Obj ecti ve: 17 10 Disc uss the con cep ts of agg luti nati on and pre cipit atio

n and whe n eac

h is app ropr iate

27 Whe

n seru

m protei

ns ar

e separatedbyelectrophoresi

s an

d thenantibodiesspecific forth

e seru

m protei

ns ar

e placed

in

a parallel troug

h

in orde

r

to for

m reactio

n ar

cs foreachprotei

n, th

e te

st

is calle

d A

B

C

D

E

Lea rnin g Obj ecti ve: 17 10 Disc uss the con cep ts of agg luti nati on and pre cipit atio n and whe

n eac

h is app ropr iate

28 Whic

h

of th

e following

is no

t anagglutinatio

n test?

A

B

D

E

Lea rnin g Obj ecti ve: 17 10 Disc uss the con cep ts of agg luti nati on and pre cipit atio n and whe n eac

h is app ropr iate

29 In precipitationtests, th

e antig

Lea rnin g Obj ecti ve: 17 10 Disc uss the con cep ts of agg luti nati on and pre cipit atio n and whe n eac

h is app ropr iate

.

30 Syphilisca

n bediagnose

d byth

e A

B

C

D

Lea rnin g Obj ecti ve: 17 10 Disc uss the con cep ts of agg luti nati on and pre cipit atio n and whe n eac

h is app ropr iate

31 The Wida

l te

st

is determina

nt for

A.B

C

D

E

Lea rnin g Obj ecti ve: 17.

10 Disc uss the con cep ts of agg luti nati on and pre cipit atio n and whe n eac

h is app ropr iate

32 The VRDLte

st detects

ts of agg luti nati on and pre cipit atio n and whe n eac

h is app ropr iate

33 Whic

h

of th

e following

is incorrec

t regardingth

e complemen

t fixationtest?

A

C

D

Lea rnin g Obj ecti ve: 17 12 Des crib e how com ple me nt fxa tion wor ks.

34 Whic

h te

st

is th

e confirmin

g te

st forpeop

le wh

o initially testedantibody-positive

in th

e screeningELISAtest?

A

the ste ps of an ELIS A and expl ain the dif ere nce bet wee

n a dire ct and an indi rect test

35 Serotypin

g

of th

e pneumococcu

s base

d oncapsul

ar polysaccharide

is performed

in A

B

D

E

Lea rnin g Obj ecti ve: 17 09 Dif ere ntia te bet wee n sen sitiv ity and spe cifc ity.

36 The Wester

n bl

ot te

st

is confirmator

y forHI

V becaus

e A

B

D

E

Lea rnin g Obj ecti ve: 17 11 List the ste ps

of a Wes tern blot

37 Whic

h te

st

is used

to diagno

se polio

? A.B

C

D

E

Lea rnin g Obj ecti ve: 17 12 Des crib

e how com ple me nt fxa tion wor ks.

38 Whic

h te

st

is used

to confirmscarl

et fever? A.B

C

D

E

Lea rnin g Obj ecti ve: 17 04 Expl ain the idea s beh ind pre sum ptiv e vs con frm ator y dat a.

39 Whic

h te

st

is used

to confirmrheumati

c fever? A.B

C

D

E

Lea rnin g Obj ecti ve: 17 04 Expl ain the idea s beh ind pre sum ptiv e vs con frm ator y dat a.

40 The Quellun

g te

st involve

E.

41 Whic

h te

st us

es radioactiveisotope

s

to labe

l antibodies

or antigen

s

in orde

r

to dete

ct minu

te amount

s

of correspondi

or antibody? A

B

C

D

E

Lea rnin g Obj ecti ve: 17 03 Expl ain the idea s beh ind pre sum ptiv e vs con frm ator y dat a Lea rnin g Obj ecti ve: 17 09 Dif ere ntia te bet wee n sen sitiv ity and spe cifc ity.

42 The dy

es fluorescei

n an

d rhodami

ne

A

B

D

E

Lea rnin g Obj ecti ve: 17 04 List at leas

t 3 dif ere nt test s that fall in the dire ct ide ntif cati on cat ego ry.

43 Horseradishperoxida

se an

d alkalinephosphatas

e ar

e enzym

es used

in _ tests A

B

C

E

Lea rnin g Obj ecti ve: 17 13 List the ste ps of an ELIS A and expl ain the dif ere nce bet wee

n a dire

ct and an indi rect test

44 A positiveELISAresul

t require

s A

B

D

Lea rnin g Obj ecti ve: 17 13 List the ste ps of an ELIS A and expl ain the dif ere nce bet wee

n a dire ct and an indi rect test

45 The indirec

t ELISAte

st

is used

to identif

y all

of th

e followingexce

pt

A

B.C

D

Lea rnin g Obj ecti ve: 17 13 List the ste ps of an ELIS A and expl ain the dif ere nce bet wee

n a dire ct and an indi rect test

46 The direc

t ELISAte

st

is used

to identif

y all

of th

e followingexce

pt A

B

C

D

Lea rnin g Obj ecti ve: 17 13 List the ste ps of an ELIS A and expl ain the dif ere nce bet wee

n a dire ct and an indi rect test

47 A simpl

e metho

d

to dete

ct _ cel

ls involve

s mixingthe

m wit

h untreatedshee

p re

d bloo

d cel

ls th

at wil

l bindan

d

m rosette

s A

48 In viv

o testing

is usef

ul fordiagnosin

Lea rnin g Obj ecti ve: 17 10 Dif

ere ntia te bet wee n sen sitiv ity and spe cifc ity.

49 The tuberculi

n ski

n te

st

is re

Lea rnin g Obj ecti ve: 17 10 Dif ere ntia te bet wee n sen sitiv ity and spe cifc ity.

50 Whe

n minu

te sampl

es

of DNAneed

to begeneticall

y analyze

d foridentificatio

n purposes,whichte

st ca

n beeffectivelyused

?

B.C

D

Lea rnin g Obj ecti ve: 17 07 Pro vide an expl ana tion for how PCR is usef ul for infe ctio us dise ase dia gno sis

True / False Questions

s.

52 Whe

n antibodies

or othe

r substanc

es

in seru

m cross-reac

t wit

h th

e te

st reagents,

a fal

se positiveresul

t ca

n occu

r

TR UE

Lea rnin g Obj ecti ve: 17 10 Dif ere ntia te bet wee n sen sitiv ity and spe cifc ity.

53 Serologica

l testingalwa

ys involve

s reactio

ns betwee

n specific antibod

y an

d antigen

TR UE

Lea rnin g Obj ecti ve: 17 08 Giv

e a thor oug h def niti on of

the ter m ser olog y.

54 Serologica

l testsshou

ld havelo

w sensitivityan

d specifici

ty

FA LS E

Lea rnin g Obj ecti ve: 17 09 Dif ere ntia te bet wee n sen sitiv ity and spe cifc ity.

55 The Weil-Fel

ix te

st

is usedfordiagnosin

g salmonellosi

s

FA LS E

Lea rnin g Obj ecti ve: 17 10 Disc uss the con cep ts of agg luti nati on and pre cipit atio n and whe n eac

h is app ropr iate