TEST BANK microbiology a systems approach cowan 3e chapter003

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?... Lea rnin g Obj ecti ve: 3.0 7

Multiple Choice Questions

1 The Five I's of studying microorganisms include all of the following except

3 A pure culture contains

A Only one species of

with some colonies on the surface?

9 Agar is an important component of media because

A Bacteria require agar to

15 A nutrient medium that contains at least one ingredient that is NOT chemically definable would be termed

A The microbiologist used too much

inoculum

B The culture is

contaminated

19 A reducing medium contains

A Sugars that can be

20 Which type of medium is able to distinguish different species or types of

microorganisms based on an observable change in the colonies or in the medium?

21 A microbiologist decides to use a nutrient medium that contains thioglycollic acid What type of microbe is she attempting to culture?

27 A microbiologist inoculates Staphylococcus aureus into a culture medium Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are

determined to be growing in this culture What is the most likely explanation?

A The microbiologist used too much

ha

t

is th

e ter

m for

a cultur

e mad

e fro

m on

e isolatedcolony?

wl

y inoculatedcultur

es mus

t be _

at

a specific temperatur

e an

d tim

e

to encourag

e grow

th

A

B

C

D

e _

of th

e microscopeholdsan

d allow

s selectio

n

of th

e objectiv

e lens

E

hic

h

of th

e followingwil

l convergelig

ht ra

ys

to

a singl

e focalpointonth

e specimen

?

A

B

C

D

E

hic

h

of th

e followingmagnifie

s th

e specimen

to produc

e th

e re

al imag

e

of th

e specimen

?

A

B

C

D

E

hic

h

of th

e followingmagnifie

s th

e specimen

to produc

e th

e virtu

al imag

e

of th

e specimen

?

A

B

C

D

E

hic

h

of th

e followingcontrolsth

e amou

nt

of lig

ht enterin

g th

e specimen

?

A

B

C

D

E

a microbiologist

is studying

a specimen

at

a tot

al magnification

of 950X, wh

at

is th

e magnifyingpowe

r

of th

e objectiv

e le

ns

if th

e ocul

ar le

ns

is 10X?

gnification

is achieved

in

a compoun

d microscopethroughth

e initia

l magnification

of th

e specimenbyth

e _ lens.Th

is imag

e

is thenprojecte

d

to th

e _ le

ns th

at wil

l furth

er magnif

y th

e specimen

to for

m

a virtu

al im

e ey

37 Whic

h

of th

e followingcharacteristi

cs refer

s

to th

e microscope's abilit

y

to showtw

o separat

e entiti

es

as

38 Allof th

e followingar

e diameter

s

of cel

ls th

at woul

d beresolve

d

in

a microscopewit

h

a limi

t

of re

39 The wavelengt

h

of lig

ht usedan

d th

e numericalapertur

e govern

s

A

B

40 _ ha

s th

e sam

e optic

al qualitie

s

as glas

s an

d th

us preven

ts refractivelos

s

of lig

ht

as

it pass

es fro

m

e le

41 The type

of microscope

in whichyo

u woul

d se

e brightlyilluminate

d specimen

s agains

t ablac

k back

C

D

E

42 Whic

h type

of microscopeshow

s cel

ls agains

t abrigh

t backgroundan

d als

o show

s intracellul

ar structur

es

d ontheirvaryingdensities?

43 Whic

h type

of microscope

is th

e mos

t wide

ly usedan

d show

s cel

ls agains

t abrigh

t background

?

D

E

44 Allof th

e followingperta

in

to th

e fluorescenc

e microscopeexce

pt

A

D

E

confoc

al scanningoptic

al microsco

pe

A

B

C

46 A confoc

al scanningoptic

al microsco

pe

A

D

E

hic

h type

of microscopedo

es no

t us

e lig

ht

in formingth

e specimenimage?

A

B

C

D

E

hic

h type

of microscopeachiev

es th

e greate

st resolutionan

d highes

t magnification

?

A

B

C

D

E

hic

h type

of microscopecann

ot imag

e liv

e specimens?

hic

h type

of microscopebombard

s awhol

e, metal-coatedspecimenwit

h electro

ns movingba

ck an

d for

th ov

er it?

A

B

C

D

E

e specimenpreparationth

at

is be

st forviewingcel

l motilit

E

e purpos

e

of stainingcel

ls on

a microscopeslide

E

e gra

m stainingprocedur

e

is be

st described

as

a stainingtechniqu

e

A

B

C

D

ha

t doth

e Gra

m stai

n, acid-fas

t stai

n an

d endospor

e stai

n have

in common

?

A

B

C

55 Basicdy

es ar

56 A microbiologistmak

es

a fixedsmea

r

of bacteri

al cel

ls an

d stai

ns the

m wit

h Loefer'

s methylenebl

ar blueunde

r th

e oil lens.Th

is

is anexampl

E

Matching Questions

tching.1.)

1 Km A.)10-2 cm2.)

1 mm B.)10

0 mm

3.)10cm C.) 10-6 m4.)

1 m D.) 1000m

hoinventedth

e fir

st crud

e microscopebygrindin

g glass?

A

B

C

D

59 Whic

h type

of med

ia woul

d beth

e be

st choic

e whenshippin

g

a sampl

e

of bacteri

a

to

a laborator

y

to

e of

ce sit

60 The Gra

m stainingprocedur

e

is be

st described

as

a stainingtechniqu

e

A

D

True / False Questions

61 The procedur

es forculturin

g

a microorganis

m requireth

e us

e

of

a microscope

mediu

m th

at

is gel-lik

e ha

s les

s ag

ar

in

it compare

d

to

a sol

id mediu

m

Tr

ue False

63 Som

e microb

es ar

e no

t capabl

e

of growin

g onartificial med

ia

Tr

ue False

selectiv

e mediu

m contai

ns on

e

or mor

e substanc

es th

at inhibitgrow

th

of certa

in microb

es

in orde

r

to facilitat

e th

e

th

of othe

r microb

es

Tr

ue False

e colon

y typicall

y develop

s fro

m th

e grow

th

of severalpare

nt bacteri

al cel

ls

Tr

ue False

66 Mixe

d cultur

es ar

e als

o referred

to

as contaminate

d cultur

es

Tr

ue False

cteri

al cultur

es ar

e easil

y identifiedfro

m theirmicroscopicappearan

ce

Tr

ue False

68 Norm

al incubationtemperatur

es rang

e fro

m 30

°

to 60

°

C

Tr

ue False

e bendin

g

of lig

ht ra

ys

as theypa

ss for

m on

e mediu

m

to anothe

r

is calle

d refraction

Tr

ue False

70 The re

al imag

e

is th

e revers

e

of th

e actu

al specimen

Tr

ue False

differenti

al interferen

ce contras

t microscopeus

es dy

es

to givecoloredthree-dimensiona

l imag

es

Tr

ue False

72 Fixedsmea

rs

of specimen

s ar

e require

d

in orde

r

to performth

e Gra

m stai

n an

d endospor

e stai

n on

ue False73 At th

e en

d

of th

e Gra

m stai

n, gra

m positivebacteri

a wil

l beseen

as pinkcel

ls

Tr

ue False

Chapter 003 Tools of the Laboratory: The Methods for Studying

Microorganisms Key

Multiple Choice Questions

e Fiv

e I's

of studyingmicroorganismsincludeall

of th

e followingexce

pt A

B

C

D

E

Lea rnin g Obj ecti ve: 3.0 1 Expl ain wha t the

5 I's me an and wha t eac h ste p ent ails.

2 Th

e ter

m th

at refer

s

to th

e purposef

ul additio

n

of microorganismsint

o

a laborator

y nutrien

t m

D

E

Lea rnin g Obj ecti ve: 3.0 1 Expl ain wha t the

5 I's me an and wha t eac h ste p ent ails.

re cultur

e contai

Lea rnin g Obj ecti ve: 3.0 1 Expl ain wha t the

5 I's me

4 Th

e corre

ct microbiologica

l ter

m forth

e tin

y sampl

e

of specimenth

at

is pu

t in

o

a nutrien

t mediu

m

e

a cultur

e

is th

e A

B

C

D.E

Lea rnin g Obj ecti ve: 3.0 1 Expl ain wha t the

5 I's me an and wha t eac

h ste p ent ails.

5 Whic

h

of th

e following

is essenti

al fordevelopmen

t

of discret

e, isolatedcolonies

? A

D

E

Lea rnin g Obj ecti ve: 3.0 2 Na me and def ne the 3 way

s to cat ego rize me dia.

h metho

d oftenresul

ts

in coloniesdevelopin

g downthroughou

t th

e ag

ar alon

g wit

h som

e coloniesonth

e surface

?

for eac

h of the 3 cat ego ries of me dia.

t type

of isolatio

n techniqu

e

is mos

t effectiv

e forth

e majori

ty

of applications

? A

B

C

E

Lea rnin g Obj ecti ve: 3.0 1 Expl ain wha t the

5 I's me an and wha t eac h ste p ent ails.

8 Whic

h

of th

e followingwil

l resul

t when1

%

to 5

% ag

ar

is adde

d

to nutrien

t brot

h, boile

d an

d

C

D

E

Lea rnin g Obj ecti ve: 3.0 1 Expl ain wha t the

5 I's me an and wha t eac h

ste p ent ails.

9 Ag

ar

is animporta

nt compone

nt

of med

ia becaus

e A

B

Lea rnin g Obj ecti ve: 3.0 1 Expl ain wha t the

5 I's me

an and wha t eac h ste p ent ails.

10 The threephysicalforms

of laborator

y med

ia ar

e A

B

Lea rnin g Obj ecti ve: 3.0 2 Na me and def ne the 3 way

s to cat ego rize me dia.

11 Whic

h

of th

e following

is no

t aninoculatingtool?

A

B.C.D.E

Lea rnin g Obj ecti ve: 3.0

h ste p ent ails.

12 Agar

is

a complexpolysaccharideth

at com

es fro

m a/

rnin g Obj ecti ve: 3.0 2 Na me and def ne the 3 way

s to cat ego rize me dia.

13 Whic

h

of th

e following

is no

t abenefit

of ag

ar

as

a sol

id medium

? A

B

E

Lea rnin g Obj ecti ve: 3.0 2 Na me and

def ne the 3 way

s to cat ego rize me dia.

14 A nutrien

t mediu

m th

at ha

s all

of itschemicalcomponent

s identifiedan

d theirpreci

se concentratio

ns kn

e, woul

d beterm

es for eac

h of the 3 cat ego ries of me dia.

15 A nutrien

t mediu

m th

at contai

ns

at leas

t on

e ingredie

nt th

at

is NOTchemical

ly definab

le woul

d be

D

E

Lea rnin g Obj ecti ve: 3.0 3 Pro vide exa mpl es for eac

h of the 3 cat ego ries of me dia.

of th

e followingar

e exampl

es

of differe

nt type

s

of microbiologica

l med

ia exce

pt A.B

C

D

Lea rnin g Obj ecti ve: 3.0 3 Pro vide exa mpl es for eac

h of the 3 cat ego ries of me dia.

17 A microbiologistinoculat

es Staphylococcu

s epidermidisan

d Escherichi

a col

i int

o

a cultur

e medium.Fol

ly th

e

E col

i grow

s

in th

e cultur

e Wha

t

is th

e mos

t likelyexplanation

? A

C

Lea rnin g Obj ecti ve: 3.0 1 Expl ain wha t the

5 I's me an and wha t eac h ste p ent ails.

commonmediu

m usedforgrowin

g fastidio

us bacteri

Lea rnin g Obj ecti ve: 3.0 3 Pro vide exa mpl es for eac

h of the 3 cat ego ries of me dia.

19 A reducin

g mediu

m contai

ns A

B

C

Lea rnin g Obj ecti ve: 3.0 3 Pro vide exa mpl es for eac

h of the 3 cat ego ries of me dia.

hic

h type

of mediu

m

is ab

le

to distinguishdiffere

nt speci

es

or type

s

of microorganismsbase

d onanobservab

le change

in

e colonies

or

in th

e medium

ego ries of me dia.

microbiologistdecid

es

to us

e

a nutrien

t mediu

m th

at contai

ns thioglycolli

c aci

d Wha

t type

of microb

e

is sh

e attem

to culture? A

22 Differenti

al med

ia resul

ts

in which

of th

e followinggrow

th characteristics

? A

E

Lea rnin g Obj ecti ve: 3.0 3 Pro vide exa mpl es for eac

h of the 3 cat ego ries of me dia.

reducin

g med

ia

is used

to cultur

e A

B

C

D

Lea rnin g Obj ecti ve: 3.0 3 Pro vide exa mpl es for eac

h of the 3 cat ego ries of me dia.

24 For whichbacteri

al genu

s

is mannit

ol sal

t ag

ar selective? A

B

C

D

rnin g Obj ecti ve: 3.0 3 Pro vide exa mpl es for eac

h of the 3 cat ego ries of me dia.

microbiologistmus

t cultur

e

a patient'

s fece

s forintestin

al pathogen

s Whic

h

of th

e followingwoul

d likelybepresen

t

in selectiv

e med

ia foranalyzin

g thi

s fecalspecimen

? A.B

C

D

rnin g Obj ecti ve: 3.0 3 Pro vide exa mpl es for eac

h of the 3 cat ego ries of me dia.

cteri

a th

at requirespeci

al grow

th factor

s an

d complexnutrien

ts ar

e term

ed

A.B

C

D

Lea rnin g Obj ecti ve: 3.0 3 Pro vide exa mpl es for eac

h of the 3 cat ego ries of me dia.

27 A microbiologistinoculat

es Staphylococcu

s aure

us int

o

a cultur

e medium.Followingincubation, bo

th St

us an

d Staphylococcu

s epidermidisar

e determine

d

to begrowin

g

in thi

s cultur

e Wha

t

is th

e mos

t likelyexplanation

? A.

B

Lea rnin g Obj ecti ve: 3.0 3 Pro vide exa mpl es for eac

h of the 3

cat ego ries of me dia.

28 Wha

t

is th

e ter

m for

a cultur

e mad

e fro

m on

e isolatedcolony? A.B.C.D

rnin g Obj ecti ve: 3.0 1 Expl ain wha t the

5 I's me an and wha t eac h ste p ent ails.

wl

y inoculatedcultur

es mus

t be _

at

a specific temperatur

e an

d tim

e

to encourag

e grow

th

A

B

C

D

Lea rnin g Obj ecti ve: 3.0 1 Expl ain wha t the

5 I's me an and wha t eac h ste p ent ails.

30 The _

of th

e microscopeholdsan

d allow

s selectio

n

of th

e objectiv

e lens

es

A.B

Lea rnin g Obj ecti ve: 3.0 5 Des crib e the earl iest mic rosc ope s Lea rnin g Obj ecti ve: 3.0 7 Dif ere ntia te bet wee n the prin cipl es of ligh t and elec tron mic rosc opy.

hic

h

of th

e followingwil

l convergelig

ht ra

ys

to

a singl

e focalpointonth

e specimen

? A

B

C

D

E

Lea rnin g Obj ecti ve: 3.0 7 Dif ere ntia te bet wee n the prin cipl es of ligh t and elec tron mic rosc

32 Whic

h

of th

e followingmagnifie

s th

e specimen

to produc

e th

e re

al imag

e

of th

e specimen

?

Lea rnin g Obj ecti ve: 3.0 7 Dif ere ntia te bet wee n the prin cipl es of ligh t and elec tron mic rosc opy.

hic

h

of th

e followingmagnifie

s th

e specimen

to produc

e th

e virtu

al imag

e

of th

e specimen

? A

B

D.E

Lea rnin g Obj ecti ve: 3.0 7 Dif ere ntia te bet wee n the prin cipl es of ligh t and

34 Whic

h

of th

e followingcontrolsth

e amou

nt

of lig

ht enterin

g th

e specimen

? A

E

Lea rnin g Obj ecti ve: 3.0 7 Dif ere ntia te bet wee n the prin cipl es of ligh t and elec tron mic rosc opy.

a microbiologist

is studying

a specimen

at

a tot

al magnification

of 950X, wh

at

is th

e magnifyingpowe

r

of th

e objectiv

e le

ns

if th

e ocul

ar le

ns

is 10X?

A.B.C.D

E

Lea rnin g Obj ecti ve: 3.0 7 Dif ere ntia te bet wee n the prin cipl

36 Magnification

is achieved

in

a compoun

d microscopethroughth

e initia

l magnification

of th

e specimenby

e

is thenprojecte

d

to th

e _ le

ns th

at wil

l furth

er magnif

y th

e specimen

to for

m

a virtu

al imag

e receive

d by

e ey

e A

B

C

D

E

Dif ere ntia te bet wee n the prin cipl es of ligh t and elec tron mic rosc opy.

hic

h

of th

e followingcharacteristi

cs refer

s

to th

e microscope's abilit

y

to showtw

o separat

e entiti

es

as separat

e an

d dis

tinct?

Dif ere ntia te bet wee n the prin cipl es of ligh t and elec tron mic rosc opy.

of th

e followingar

e diameter

s

of cel

ls th

at woul

d beresolve

d

in

a microscopewit

h

a limi

t

of resolution

of 0.2µ

m

pt A

the prin cipl es of ligh t and elec tron mic rosc opy.

e wavelengt

h

of lig

ht usedan

d th

e numericalapertur

e govern

s A

B

C

D

Lea rnin g Obj ecti ve: 3.0 7 Dif ere ntia te bet wee n the prin cipl es of ligh t and elec tron mic rosc opy.

40 _ ha

s th

e sam

e optic

al qualitie

s

as glas

s an

d th

us preven

ts refractivelos

s

of lig

ht

as

it pass

es fro

m

e le

n the prin cipl es of ligh t and elec tron mic rosc opy.

41 The type

of microscope

in whichyo

u woul

d se

e brightlyilluminate

d specimen

s agains

t ablac

k back

D

E

Lea rnin g Obj ecti ve: 3.0 7 Dif ere ntia te bet wee n the prin cipl es of ligh t and elec tron mic rosc opy.

hic

h type

of microscopeshow

s cel

ls agains

t abrigh

t backgroundan

d als

o show

s intracellul

ar structur

es

of unstaine

d cel

ls base

d ontheirvaryingdensities? A

B

C

D

E

Dif ere ntia te bet wee n the prin cipl es of ligh t and elec tron mic rosc opy.

hic

h type

of microscope

is th

e mos

t wide

ly usedan

d show

s cel

ls agains

t abrigh

t background

?

A

B

D

E

Lea rnin g Obj ecti ve: 3.0 7 Dif ere ntia te bet wee n the prin cipl es of ligh t

44 Allof th

e followingperta

in

to th

e fluorescenc

e microscopeexce

pt

A

D.

Lea rnin g Obj ecti ve: 3.0 7 Dif ere ntia te bet wee n the prin cipl es of ligh t

45 A confoc

al scanningoptic

al microsco

pe

A

D.

Lea rnin g Obj ecti ve:

of ligh t and elec tron mic rosc opy.

confoc

al scanningoptic

al microsco

pe

A

B

D

Lea rnin g Obj ecti ve: 3.0 7 Dif ere ntia te bet wee n the prin cipl es of ligh t and elec tron mic rosc opy.

hic

h type

of microscopedo

es no

t us

e lig

ht

in formingth

e specimenimage? A

B

C

D

E

Lea rnin g Obj ecti ve: 3.0 7 Dif ere ntia te bet wee n the prin cipl es of ligh t and elec tron mic rosc opy.

48 Whic

h type

of microscopeachiev

es th

e greate

st resolutionan

d highes

t magnification

? A