ELECTROCHEMICAL DNA BIOSENSORS phần 6 pps

Hybridization detection procedure... z The guanine signal obtained from the hybridization of the Mutant type-1 probe with the amplicons6 and 1 a R... Development of an SPR-based biosens

z Wild Type Capture Probe-1(WTCP): (It is specifically bound with wild type HBV)

5’ – AAT ACC ACA TCA TCC ATA TA – 3’

z Mutant Type-1 Capture Probe(MT-1CP): (It is specifically bound with mutant type-1 HBVgenome-YVDD)

5’ – AAT ACC ACA TCA TCC AcA TA – 3’

z Mutant Type-2 Capture Probe(MT-2CP):( It is specifically bound with mutant type-2 HBVgenome-YIDD)

5’ – AAT ACC ACA TCA TCa ATA TA – 3’

z Wild type target sequence-1(WTT=YMDD):

5’- TAT ATG GAT GAT GTG GTA TT-3’

z Mutant type-1 target sequence-2(MT-1T=YVDD):

5’- TAT gTG GAT GAT GTG GTA TT-3’

z Mutant type-2 target sequence-3(MT-2T=YIDD):

5’- TAT ATt GAT GAT GTG GTA TT-3’

z Non comlpementary sequences:

5’ – AAT ACC TGT ATT CCT CGC CTG TC –3’

Hybridization detection procedure

Cloned PCR products

z The guanine signal obtained from the wild type probe modified PGE after hybridization with samples 1, 2 and 3 gave a R S D value of 9.4%, 7.6% and 10.2%

z The guanine signal obtained from the hybridization of the Mutant type-1 probe with the amplicons(6 and 1) a

R S D value of 10.4% and 11.8%

z The guanine signal obtained from the hybridization of the Mutant type-2 probe with the samples 8 and 9 gave a

R S D value of 7.4 % and 6.0% The detection limits, estimated from S /

N = 3, correspond to 457 fmole / mL using 6µg/ml concentration level of target at PGE

Development of an SPR-based biosensor for the detection of lipophilic

phycotoxins in shellfish residues

A European Commission-funded Specific Targeted Research Project

Food Quality and Safety

Food- CT - 2004- 514055

Biosensors and their

application to improving food safety and quality

Biosensors and their

application to improving food safety and quality

The Classic Binding Molecule:

The Antibody

The Classic Binding Molecule:

The Antibody

IgG MW ~180,000

SPR

Photo-1: (left to right) Assoc Prof.Arzum ERDEM, Prof

Mehmet OZSOZ, PhD.Std Kagan KERMAN, PhD Std Pinar

KARA, PhD.Std Dilsat OZKAN

PhD.Std.Burcu MERIC, PhD Std Pinar KARA, Assoc Prof.Arzum ERDEM, PhD.Std Dilsat

OZKAN, PhD.Std Kagan KERMAN

to right) Prof

Mehmet

OZSOZ and ,

PhD Std

Hakan

KARADENIZ