ELECTROCHEMICAL DNA BIOSENSORS phần 4 docx

Disposable genosensor, a new tool for the detection ofNOS-terminator, a genetic element present in GMOs B.. / Food Control 15 2004 621–626 zGenetically modified organisms GMOs are referr

Disposable genosensor, a new tool for the detection of

NOS-terminator, a genetic element present in GMOs

B Meric et al / Food Control 15 (2004) 621–626

zGenetically modified organisms (GMOs) are referred to as living organisms of vegetable or animal origin in which the artificial

introduction of foreign genes is able to confer novel

characteristics not

found in the wild type

The EU Council regulation on traceability and labeling for GMO

- A threshold of 0.9% is set for labeling, below which labeling of GMOs is not required

z PCR methods, based on exponential

amplification of a specific GMO DNA fragment , are highly sensitive, but require sophisticated instruments for post-amplification steps, such

as gel electrophoresis and fluorescent

imaging.

- Alternatively an electrochemical genosensor is presented here for rapid analysis of GMO

related PCR products.

Since GMOs and their derivatives are widely used

in food industry, the analytical assays are needed

to provide the detection and the quanti.cation of

GMOs in food matrices

z Nos terminator is present in nearly all GMOs due to its important in gene

regulation

zDNA extraction and PCR was performed

zFrom the amplification of the NOS-terminator region,fragments of 180 bp (base pairs) were obtained

Probe 5`-AAT GAT TAA TTG GGG GAC TCT AAT C-3’

Target 5’-GAT TAG AGT CCC CCA ATT AAT CAT T-3’

Carbon Nanotubes(CNT)

were used as nanowires which combined DNA molecules to a carbon paste

electrode(CPE)

properties and chemical stability

Thymine probe: 5’-Amino-TTT TTT-3’

Adenine probe: 5’-Amino-AAA AAA-3’

Inosine substituted thymine tagged probe:

5’-TTT TTTCAA IAC CAC CAC TTC IAA ACC-3’ Target: 5’-GGT TTC GAA GTG GTG GTC TTG-3’ Single-base mismatch:

5’-GGT TTC GAA GGG GTG GTC TTG-3’

DNA-Directed Attachment of Carbon Nanotubes for Enhanced Label-Free Electrochemical Detection of DNA Hybridization

Electroanalysis DOI: 10.1002/elan.200303025

Electrochemical Coding of

Single-Nucleotide Polymorphisms By Monobase-Modified

Gold Nanoparticles

Anal Chem.2004, 76,1877-1884

Electrochemical Genosensor for the Discrimination of HSV

(Herpes Simplex Virus)

Type I and II

Analytica Chimica Acta 518 (2004) 69–76