The visible color shift and aggregation of oligonucleotide modified Au nanoparticles upon binding to target DNA is a well-described event.. "Selective Colorimetric Detection of Polynucl
Trang 1The visible color shift and aggregation of oligonucleotide
modified Au nanoparticles upon binding to target DNA is a
well-described event
Elghanian, R.; Storhoff, J J.; Mucic, R C.; Letsinger, R L.; Mirkin, C A "Selective
Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical
Properties of Gold Nanoparticles," Science, 1997, 277, 1078-1080.
Color shift is only observed from the hybridization with the
target DNA
Trang 4Au Electrode Study Procedure
Trang 5Electrode system
Trang 6DNA-Chip technology
Trang 7Chip Design
Trang 8What is Factor V Leiden
Mutation?
position of the human genome.
Trang 9Experimental Procedure
z When hybridization was occured between probe and target
on CPE surface, a guanine oxidation signal at ~+1.00 V was appeared The YES / NO system was established for the electrochemical detection of allele – specific mutation
on Factor V
Trang 10THE DETECTION OF “ALLEL
SPECIFIC” GENE