ELECTROCHEMICAL DNA BIOSENSORS Prof.. of Analytical Chemistry, Faculty of Pharmacy, Ege University 35100 Bornova /Izmir, TURKEY ozsozs@pharmacy.ege.edu.tr http://pharmacy.ege.edu.tr/~oz
Trang 1ELECTROCHEMICAL DNA
BIOSENSORS
Prof Dr Mehmet OZSOZ
Dept of Analytical Chemistry,
Faculty of Pharmacy, Ege University
35100 Bornova /Izmir, TURKEY
ozsozs@pharmacy.ege.edu.tr http://pharmacy.ege.edu.tr/~ozsozs
Trang 2Imagination is more important than knowledge.
Albert Einstein
Trang 3z If a man emties his purse into his head,
no man can take it away from him An
investment in knowledge always pays the best interest.
Benjamin Franklin
Trang 4PRESENTATION OVERVIEW
z Electrochemical DNA Hybridization Sensing Strategies
z Inosine based hybridization detection by using carbon paste electrode (CPE)
z Detection of NOS-terminator, a genetic
element present in GMOs
z Carbon Nanotubes
z Optical Biosensors
Trang 5z If a man emties his purse into his head,
no man can take it away from him An
investment in knowledge always pays the best interest.
Benjamin Franklin
Trang 6DNA biosensor scheme
Trang 7Basic principle of
a glucose biosensor
GOX
Transducer Analytical signal
Trang 8K 3 Fe(CN) 6
Screen printed electrode
Working electrode reactions pathway
ChOx layer
Electrochemical mediator
Electrode surface (-50mV vs pseudo Ag/AgCl)
H 2 O 2
Fe (III) Å Fe (II)
Fe (III) + e - Æ Fe (II)
Acetylcholine + H2O Æ Acetate +Choline
(AChE)
ChOx
Organaphosphate assay
Trang 10Electrochemical DNA Hybridization
Sensing Strategies
1.Label based
a) Hybridization indicators
b) Labelled probe
2 Label free
guanine, (Inosine), adenine