What is Micropropagation?“… the asexual or vegetative propagation multiplication of plants in vitro “ Implies - regeneration - multiplication - uniformity ??... Selection of plant mat
Trang 1Plant Tissue Culture
Part 3 - Micropropagation
Richard R Williams
Trang 2What is Micropropagation?
“… the asexual or vegetative propagation
(multiplication) of plants in vitro “
Implies - regeneration
- multiplication
- uniformity ??
Trang 3Basic in vitro propagation
Trang 41 Selection of plant material
2 Establish aseptic culture
3 Multiplication
4 Shoot elongation
5 Root induction / formation
6 Acclimatization
Trang 5Benefits for propagation
♣ Rapid multiplication of clones
Trang 71 Selection of plant material
2 Establish aseptic culture
3 Multiplication
4 Shoot elongation
5 Root induction / formation
6 Acclimatization
Trang 8Starting material for micropropagation
Trang 9[Drew et al 1991]
Trang 10Selection of plant material
Trang 11Physiological state - of stock plant
Trang 121 Selection of plant material
2 Establish aseptic culture
3 Multiplication
4 Shoot elongation
5 Root induction / formation
6 Acclimatization
Trang 16Physical Environment
♣ Temperature
♣ Moisture
♣ Light
Trang 171 Selection of plant material
2 Establish aseptic culture
3 Multiplication
4 Shoot elongation
5 Root induction / formation
6 Acclimatization
Trang 18Origins of new shoots
♣ Terminal extension
♣ Lateral / Axillary buds
♣ Adventitious (de novo, re-differentiation)
♣ Callus differentiation
Trang 19Role of growth regulators
Trang 201 Selection of plant material
2 Establish aseptic culture
3 Multiplication
4 Shoot elongation
5 Root induction / formation
6 Acclimatization
Trang 21Shoot elongation
♣ Basal ‘hormone free’ medium
♣ Gibberellins
♣ Carry-over of hormones
Trang 221 Selection of plant material
2 Establish aseptic culture
3 Multiplication
4 Elongation
5 Root induction / formation
6 Acclimatization
Trang 241 Selection of plant material
2 Establish aseptic culture
3 Multiplication
4 Elongation
5 Root induction / formation
6 Acclimatization
Trang 25- survival of the new plant when removed from the in vitro environment
- will be covered later
Acclimatization (hardening)
Trang 26END of Part 3