Chapter 3 dac hieu enzyme e

PowerPoint Presentation Enzyme specificity Absolute Linkage digestive enzyme Group carboxypeptidase Stereo malatdehydratase 2 Absolute specificity Urease Arginase Renin Application 3 Lipase Protease A[.]

Enzyme specificity

• Absolute

• Linkage: digestive enzyme

• Group: carboxypeptidase

• Stereo: malatdehydratase

Absolute specificity

• Urease

• Arginase

• Renin

Application

• Lipase

• Protease

• Amilase (α- , β- , -)

Application

Linkage

• Aminpeptidase

• Carboxypeptidase

• NADH dehydrogenase

stereospecific

3 Hydrolase

– 3.1 Esterase

• 3.1.1 Ester carbocylic

– 3.2 Glucosidase

– 3.3 Etherase

– 3.4 Peptidase

– 3.5 Amidase

Enzym code: 4 digits

Lipase (EC 3.1.1.3)

Trypsin (EC 3.4.21.4)

Alcoholdehydrogenase (EC 1.1.1.1) Aldolase (EC 4.1.2.13)

Catalase (EC 1.11.1.6)

Starch hydrolyzing enzyme

• α- amilase: most thermal stable,

• β- amilase: acid stable

• - amilase: working pH: 3,5-5,5 Temp max: 700C

peptide hydrolase

• Endo-peptidase (proteinase): pepsin, trypsin, chimotrypsin

– Serin Protease

– Thiol Protease

– Acid Protease (aspartic proteinase)

– Metalo Protease

• Exo-peptidase (peptidase)

– Amino peptidase

– Cacboxyl peptidase

Application of hydrolysing enzyme

• amilase (-,-,-amilase)

• protease (animal, plant,

microorganism)

• Lipase

• Pectinase

• Cellulase

• Others

Oxydoreductase

• Dehydrogenase

• Polyphenoloxidase

• Catalase

• Peroxidase