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More than 70% of the buffaloes in the world belong to the River type.[2] MEAT, MILK, AND DRAFT ATTRIBUTES In general, the River types are mainly used for milk inSouth Asian countries, wh

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rumen; the remaining proportion is presumably directed to

the omasum Within the remainder of the ruminant gut,

quantitative net water absorption is greatest in the

proximal small intestine, followed by the omasum and

large intestine.[5]Recent data[6]indicate that the lower net

water absorption in the large intestine by cattle than by

sheep results from a reduced ability to retain absorbed

water because more absorbed solvent/solute is drawn back

into the lumen through the larger paracellular pores

between colonic cells in cattle

TRANSPORTATION-INDUCED

DEHYDRATION

Transport of animals on semitrailer trucks from the site of

birth to the site of growing and finishing can involve

periods of up to 24 hours or more without access to water,

and variable magnitudes of dehydration can occur Feeder

calves seem to lose approximately 3.3% of body weight

during the loading and unloading process and can lose an

additional 0.3 to 0.4% of body weight/hour of

trans-port.[7,8]Weight losses of feeder pigs during transport can

be up to 0.6% of body weight/hour.[9] Loss of

gastroin-testinal tract contents and carcass weight has accounted

for 48 and 32%, respectively, of transport shrink by feeder

steers[8]and has accounted for 62 and 27%, respectively,

of transport weight loss by feeder pigs Feces, urine, and

respiration accounted for 12.6, 26, and 60% of the water

loss.[10] Water accounted for 80% of weight lost by

wethers during 48 hours of feed and water deprivation.[11]

Of total body water loss, 57% was from the intracellular

compartment and 29% was from the gastrointestinal tract

In steers deprived of water for 4 days, thiocyanate space

(assumed to be extracellular space) accounted for 47% of

the weight lost (total loss = 16% of body weight).[12]

Thiocyanate space decreased 23% and plasma volume

decreased 28% during the 4-day period without water The

exchange of water within the body in response to

dehydration is depicted in Fig 2

Water Mineral CompositionDrinking water is a source of various minerals that aregenerally readily available for absorption unless com-plexed by an interfering nutrient Minerals ingested inwater and feed are a variable mix of positively andnegatively charged ions that contribute to the dietarycation anion difference of consumed material (DCAD)and have a direct influence on fluid and acid basebalance The DCAD is calculated as the milliequivalents(mEq) of Na+, K+, Ca+ +, and Mg+ +minus the mEq of Cl ,

S=, and P=.[13]As anion consumption and concentration inthe body increase, cellular acidosis can occur As theDCAD increases from negative to positive (e.g., 20 to+ 100 mEq/kg), feed intake and performance are generallyincreased However, the prepartum dairy cow is oneexception to the generalization Inducing mild metabolicacidosis by feeding anionic diets before calving has been

an effective means of preventing milk fever by ating calcium resorption from bone before the dramaticcalcium needs at parturition arise.[13]

potenti-Few data are available on the contribution of waterminerals to overall DCAD Socha et al.[14] reportedaverage mineral profiles of more than 3600 drinking water

Fig 2 Water change between body compartments duringdehydration Water is osmotically drawn from transcellular andintracellular compartments to interstitial and plasma compartments during dehydration, in response to losses by urinary andfecal excretion and insensible routes The magnitude ofreduction in compartmental volumes is dependent on the degree

of dehydration

Fig 1 Typical body water compartments (% of total body

water)

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samples collected across the United States Assuming that

a growing feedlot steer weighing approximately 300 kg

and consuming 9 kg of a mixed diet (>85% dry matter)

meeting mineral requirements would drink 30 L of water/

day,[2]this steer would consume twice as much weight in

water compared to the weight of feed consumed, and

approximately 3 to 7% of calcium, sodium, and sulfur

consumed would be derived from water However,

ap-proximately 20% of chloride consumed would be derived

from drinking water in this example The DCAD

cal-culated for the surveyed samples[14] was approximately

0.4 mEq/kg Estimates of the contribution of drinking

water minerals to overall DCAD are needed

CONCLUSION

The polarity and ability of water to facilitate hydration of

polar and ionic molecules are central to the flow of water

and metabolites within the body Saliva appears to be a

greater proportion of ruminal fluid than previously

thought, considering recent observations that some water

consumed by drinking in nonsuckling cattle bypasses the

rumen, but more intensive study is needed The ability of

sheep to form drier feces than cattle results from tighter

junctions between colonic cells and a greater ability to

establish an osmotic gradient to retain absorbed water

Cattle may lose approximately 3% of body weight during

loading and unloading for transport, plus an additional 0.3

to 0.4% of body weight per hour of transport Indirect data

suggest that water may constitute up to 80% of this weight

loss Estimates of the contribution of drinking water

minerals to overall cation anion difference and of the

influence of water cation anion difference on animal

performance are needed

REFERENCES

1 Bohinsky, R.C Modern Concepts in Biochemistry, 5th Ed.;

Allyn and Bacon, Inc.: Boston, MA, 1987

2 Parker, D.B.; Brown, M.S Water Consumption forLivestock and Poultry Production In Encyclopedia ofWater Science, 1st Ed.; Stewart, B.A., Howell, T.A., Eds.;Marcel Dekker, Inc.: New York, NY, 2003

3 Christopherson, R.J.; Webster, A.J.F Changes duringeating in oxygen consumption, cardiac function and bodyfluids of sheep J Physiol 1972, 221, 441 457

4 Zorrilla Rios, J.J.; Garza, D.; Owens, F.N Fate ofDrinking Water in Ruminants: Simultaneous Comparison

of Two Methods to Estimate Ruminal Evasion; AnimalScience Research Report MP 129; Oklahoma AgriculturalExperiment Station: Stillwater, OK, 1990; 167 169

5 Sklan, D.; Hurwitz, S Movement and absorption of majorminerals and water in ovine gastrointestinal tract J DairySci 1985, 68, 1659 1666

6 McKie, A.T.; Goecke, I.A.; Naftalin, R.J Comparison offluid absorption by bovine and ovine descending colon invitro Am J Physiol 1991, 261, G433 G442

7 Bartle, S.J.; Preston, R.L Feedlot Cattle ReceivingExperiments, 1988 89; Animal Science Research Report

# T 5 263; Texas Tech University: Lubbock, TX, 1989;

28 30

8 Self, H.L.; Gay, N Shrink during shipment of feeder cattle

J Anim Sci 1972, 35, 489 494

9 Jesse, G.W.; Weiss, C.N.; Mayes, H.F.; Zinn, G.M Effect

of marketing treatments and transportation on feeder pigperformance J Anim Sci 1990, 68, 611 617

10 Mayes, H.F.; Hahn, G.L.; Becker, B.A.; Anderson, M.E.;Nienaber, J.A A report on the effect of fasting andtransportation on liveweight losses, carcass weight lossesand heat production measures of slaughter hogs Appl.Eng Agric 1988, 4, 254 258

11 Cole, N.A Influence of a three day feed and waterdeprivation period on gut fill, tissue weights, and tissuecomposition in mature wethers J Anim Sci 1995, 73,

2548 2557

12 Weeth, H.J.; Sawhney, D.S.; Lesperance, A.L Changes inbody fluids, excreta and kidney function of cattle deprived

of water J Anim Sci 1967, 26, 418 423

13 Goff, J Factors to Concentrate on to Prevent PeriparturientDisease in the Dairy Cow, Proceedings of the Mid SouthRuminant Nutrition Conference, Texas Agricultural Extension Service: College Station, TX, 1998; 63

14 Socha, M.T.; Ensley, S.M.; Tomlinson, D.J.; Ward, T.Water composition variability may affect performance.Feedstuffs 2003, 75 (24), 10

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Water Buffalo

Nguyen van Thu

Cantho University, Can Tho City, Vietnam

INTRODUCTION

The water buffalo is considered to be a very useful

animal in many countries, supplying draft power, meat,

milk, and other by-products such as hides, horn, etc

The water buffalo is closely associated with water or

mud, and with smallholder farmers in the rice fields

In recent years, buffalo production has developed well,

not only in Asia, but also in Europe, South America,

and other continents where the buffalo has been

introduced This article aims to introduce some basic

knowledge of the water buffalo, with an emphasis on

its great contribution to our living standards and

improved productivity that could be better exploited

for a more sustainable agriculture development in the

21st century

TAXONOMY AND TYPES

The world’s buffaloes are classified into two groups,

the African and the Asian, with genus names Syncerus

and Bubalus, respectively According to the zoological

classification,[1]buffaloes belong to the class Mammalia,

subclass Ungulata, order Artiodactila, suborder

Ruminan-tia, family Bovidae, subfamily Bovinae, tribe Bovini The

tribe Bovini includes three groups: Bovina (cattle),

Bubalina (the Asian buffalo), and Syncerina (the African

buffalo) The Asian and African buffaloes are generally

similar, but there are some anatomic differences The

African buffalo includes only one species, Syncerus

caffer, while the Asian buffalo comprises three species:

Anoa (Bubalus depressicornis) from the Island of

Celebes, Tamarao (Bubalus mindorensis) from the Island

of Mindoro, and Arni (Bubalus Arnee), or the Indian wild

buffalo Of these four species of African and Asian

buffalo, only the India Arni buffalo has been domesticated

and given the species name bubalis Therefore, the

domestic buffalo currently reared with the name of water

buffalo is classified as bubalus bubalis It is believed that

the domestication of the buffalo occurred about 5000

years ago on the Indian subcontinent, and the

domestica-tion of the Swamp buffalo took place in China about 1000

years later

The water buffalo can be classified into two breedtypes, the River type (2n = 50) and the Swamp type(2n = 48) River breeds consist of: 1) Asian breeds such

as those in India and Pakistan (including Murrah, NiliRavi, Surti, etc.; and 2) Mediterranean breeds found inItaly, Romania, and the Middle East The skin of Riverbuffaloes is black, but some specimens have a dark slate-colored skin The horns of the River buffalo growdownward and backward, then curve upward in a spiral.The Swamp type is found mainly in China and SoutheastAsia The skin of the Swamp buffaloes is gray at birth, butbecomes slate blue later Albinoid Swamp buffaloes arequite common in some areas, for example, in the north ofThailand Normally, the horns of Swamp buffaloes arelonger than those of the River buffaloes, grow outward,and curve in a semicircle More than 70% of the buffaloes

in the world belong to the River type.[2]

MEAT, MILK, AND DRAFT ATTRIBUTES

In general, the River types are mainly used for milk inSouth Asian countries, while the Swamp types are usedfor draft power in Southeast Asian countries and China(Table 1) However, both the River and Swamp types havebeen used for multiple purposes such as work, milk, meat,manure, fuel, etc by small farmers in different croplivestock farming systems In addition, crossbreedingprograms of the River and Swamp buffaloes have showngreat potential for improving meat, work, and milkoutputs Recently, the U.S Department of Agriculture(USDA) estimated the nutritional value of water buffalomeat and compared it to beef and chicken The findingsshowed that water buffalo meat has 41% less cholester-

ol, 92% less fat, and 56% fewer calories than traditionalbeef Furthermore, there are as yet no reports on theoccurrence of bovine spongiform encephalopathy (BSE),also known as mad cow disease, in buffaloes in any part

of Asia.[4]

The milk yield of the buffalo is lower than that ofcattle, and average milk production is 1500 kg per lac-tation However, some individuals can produce 3500 kgper lactation Buffalo milk has high nutritive value and

is excellent for the preparation of dairy products Using

DOI: 10.1081/E EAS 120019837 Copyright D 2005 by Marcel Dekker, Inc All rights reserved.

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buffaloes for a single purpose makes them less

compet-itive with cattle and tractors This is believed to be an

important reason for the serious decline of the buffalo

population in a number of Southeast Asian countries,

including some parts of Vietnam Alexiev reported that

the Swamp-type Wenzhou buffalo in China can give an

average milk yield of 1030 kg per 280-day lactation.[2]

Thus, milk production of the Swamp buffalo is sufficient

for family consumption In addition, the Swamp buffalo

provides draft power, and thus has potential in rural areas

of China and Southeast Asian countries In Europe and the

Near East, the main purpose of raising buffaloes is for

milk Milk can be used for liquid consumption and making

different cheeses or yogurt, particularly in Italy, where

most of the buffalo milk is used for making a well-known

cheese called Italian Mozarella, which retails at a very

high price.[5,6]

The total number of buffalo in the World in 2002 was

about 167,126,000 and it is increasing, particularly in

India, China, Brazil, etc., where the River buffaloes are

raised However, there is a serious reduction of the Swamp

buffalo population in some countries such as Thailand,

Malaysia, and Cambodia due to mechanization, slaughtering for meat, and other reasons (Table 2)

over-In many cases, knowledge from studies on cattle canalso be applied to buffalo research and practices How-ever, differences in anatomy, physiology, feeding behav-ior, reproductive characteristics, and productivity betweenthe species have been reported.[8]The water buffalo is aruminant, and the rumen reticulum of buffaloes is similar

to that of cattle However, it is heavier than in cattle and

5 10% more capacious.[9]Studies comparing buffaloes tocattle have suggested a higher feed intake, longer retentiontime of feed in the digestive tract, longer rumination, lessdepression of cellulose digestion by soluble carbohydrates,

a wider range of plant preferences, and a higher tion of cellulolytic bacteria.[9] However, some authorshave found no significant difference in feed digestibilitybetween the two species It was suggested that the betterperformance of buffaloes fed coarse fodder may not berelated to a superior capacity for fiber digestion, but ratherthat they are less discriminating against plants not readilyeaten by cattle In Colombia, cattle are sometimes firstused to graze pasture, whereafter buffaloes are allowed tograze the remaining and less desirable parts of thesward.[10]Recently, in a comparative study on cattle andSwamp buffaloes raised under the same village conditions,some authors reported higher bacteria, lower protozoa, andhigher fungal zoospore counts in Swamp buffaloes.[11]Itwas also found that the Swamp buffalo can adapt better inthe acid sulphate soil areas compared to the cattle andgoats in the Mekong delta of Vietnam

popula-Based on results of a number of studies, buffalo mightutilize protein more efficiently than cattle.[9]An ability ofbuffaloes to utilize endogenous urea more efficiently thancattle may explain in part their apparent superiority inutilizing high-fiber and low-nitrogen feed resources It isconcluded that there have been contradictory results forfiber digestion abilities of buffaloes compared to cattle.Buffaloes, however, seem to have a superior ability toconsume coarse roughage, perhaps as a result of a betterrumination capacity There is evidence that urea recyclingand purine excretion in buffaloes are different from those

in cattle, but more comprehensive studies are lacking

Table 2 Buffalo population (head) in the world and in selected countries (1970 2000)

Table 1 Plowing and harrowing performance of swamp

buffaloes in the Mekong delta, Vietnam

SexFemale

(na= 24)

Male(na= 24)

Plowing timeb

(hrs/day)

5.35 ± 0.58 5.39 ± 0.31Plowed area

(ha/pair/day)

0.29 ± 0.025 0.31 ± 0.035Harrowing timeb

(hrs/day)

5.05 ± 0.17 5.28 ± 0.30Harrowed area

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It may be concluded that the water buffalo has a great

potential to develop in the future A number of promising

buffalo farming models have been developed in Brazil,

Australia, Italy, Philippines, Colombia, etc Valuable

products of water buffaloes, such as milk, meat, draft

power, and manure, are relevant for the people and our

living environment, particularly with respect to the trend

toward organic agriculture in many parts of the world

REFERENCES

1 Alexiev, A The Water Buffalo; St Kliment Ohridski

University Press: Sofia, 1998

2 Chantalakhana, C Long term breeding strategies for ge

netic improvement of buffaloes in developing countries

Asian Aust J Anim Sci 1999, 12, 1152 1161

3 Thu, N.V A Study of Performance, Physiological Param

eters and Economic Efficiency of Working Buffaloes in the

Mekong Delta of Vietnam In Working Animals in

Agriculture and Transport; Pearson, R.A., Lhoste, P.,

Saastamoinen, M., Martin Rosset, W., Eds.; EAAP Tech

nical Series, Wageningen Academic Publisher, 2003; Vol

6, 165 171

4 Ranjhan, S.K A Vision of buffalo production with special

reference to milk and meat production Proc Symp Series

1 of the 8th World Conf Anim Prod., Seoul, Korea, June

28 July 4, 1998; 263 270

5 Borghese, A.; Moioli, B.; Tripadi, C Processing andProduct Development in Mediterranean Countries InProceedings of the Third Asian Buffalo Congress, Kandy,Sri Lanka, 2000; 37 46

6 Chantalakhana, C Long term breeding strategies forgenetic improvement of buffaloes in developing countries.Asian Aust J Anim Sci 1999, 12 (7), 1152 1161

7 FAO Live Animals FAOstat Agriculture Data; 2003.http://apps.fao.org/page/collections?subset = agriculture

8 Cockrill, W.R The Husbandry and Health of DomesticBuffalo; FAO: Rome, 1974

9 Khajarern, S.; Khajarern, J.M Feeding Swamp Buffalo forMilk Production In Feeding Dairy Cows in the Tropics;FAO Animal Production and Health Paper, WageningenAcademic Publishers: The Netherlands, 1991; Vol 86,

115 125

10 Thu, N.V A Study of the Use of Female Cattle and BuffaloCrusing Sugar Cane in Colombia M.Sc Thesis; SwedishUniversity of Agricultural Sciences: Uppsala, Sweden,Food and Agriculture of the United Nations, 1994

11 Wanapat, M.; Ngarmsang, A.; Korkhuntot, S.; Nontaso,N.; Wachirapakorn, C.; Keakes, G.; Rowlinson, P Acomparative study on the rumen microbial population ofcattle and Swamp buffalo raised under traditional villageconditions in the Northeast of Thailand Asian Aust J.Anim Sci 2000, 13 (7), 918 921

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Well-Being and Handling

Temple Grandin

Colorado State University, Fort Collins, Colorado, U.S.A

INTRODUCTION

Reducing stress during handling for procedures such as

vaccinations, milking, and herding will improve both

animal welfare and productivity Pigs and dairy cows that

are afraid of people have reduced productivity Pigs have

lower weight gains and fewer piglets and dairy cows

produce less milk Fearfulness was assessed by measuring

the animal’s willingness to approach people Cows on

dairies where the employees had received training in

stockmanship and animal behavior had a smaller flight

zone and gave more milk.[1] The trained employees

engaged in fewer negative interactions with the cows,

such as hitting or yelling Further studies have shown that

wild, excitable cattle that become highly agitated in the

squeeze chute had lower weight gains,[2] poor beef

quality, and tougher meat

BIOLOGICAL BASIS OF FEAR

Fear is a strong stressor and it can be detrimental to both

productivity and welfare People working with animals

should take steps to reduce the animal’s fear Other

stressors such as weather extremes often cannot be

avoided, but livestock producers can easily reduce fear

Fear is a basic emotion and it motivates animals to

avoid predators The amygdala is the brain’s fear center.[3]

If the amygdala is destroyed, the animal will no longer

become fearful of things that would normally cause fear,

such as sudden loud noise It also loses learned fear

responses An example of a learned fear response is

refusing to enter a squeeze chute for vaccinations because

the cow was accidentally hit on the head by the headgate

In wild animals that are not accustomed to handling,

destruction of the amygdala will make them act tame

INDICATORS OF FEARFULNESS

One indicator of fearfulness in grazing animals is the size

of the flight zone Animals with larger flight zones are

more fearful Another indicator is the startle response to

a sudden stimulus such as a firecracker Some other

behavioral indicators of fear are a cow struggling in a

squeeze chute, sweating in horses when there is littlephysical exertion, flapping in caged layers, and a horserearing when he is suddenly startled Isolation is a strongstressor, and a single cow or lamb may run into a fence ortry to jump it when it is separated from its herdmates.Physiological measures such as cortisol in the bloodcan also be used as indicators of fear stress that occursduring nonpainful restraint in a squeeze chute.[4]Cortisol

is a time-dependent measure and it takes 10 to 20 minutesfor it to reach peak levels It is important to differentiatebetween fear and pain stress Cortisol levels can also rise

in response to pain from procedures such as hot ironbranding The variable of the handling stress needs to beseparated from the variable of pain caused by a proceduresuch as castration Handling stress is mostly fear, andstress from castration is caused by pain and injury

to tissues

VARIATIONS IN HANDLING STRESSFear stress during handling can vary from almost none toextreme Extensively raised cattle that were not accus-tomed to close contact with people had much highercortisol levels when they were restrained in a squeezechute compared to hand-reared dairy cattle.[5]Taming of

an animal may reduce physiological reactivity of thenervous system Hand-reared deer that were raised inclose contact with people had significantly lower cortisollevels after restraint than free-range deer.[6]

There are three basic variables that will affect boththe intensity of fear stress during handling and the size ofthe animal’s flight zone They are: 1) genetic factors;2) amount of contact with people; and 3) previousexperiences with handling that can be either aversive

or nonaversive

GENETIC FACTORSThe domestic phenotype has reduced responses to changes

in its environment.[7] Several studies have shown thatthere are differences in how different breeds of cattle react

to handling Brahman cattle had higher cortisol levelsafter restraint than crosses of the English breeds such as

DOI: 10.1081/E EAS 120019847

Copyright D 2005 by Marcel Dekker, Inc All rights reserved.

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Hereford or Angus Some genetic lines of cattle, pigs,

or chickens are more likely to be extremely agitated

during handling

Animals that have flighty, excitable, high-fear genetics

are more likely to become highly agitated when they

are suddenly placed in a new situation, compared to

animals with a calmer temperament Flighty animals

have to be introduced more gradually to new things to

avoid agitation and panic, compared to animals with a

calmer temperament

An experiment by Ted Friend showed that

measure-ments of epinephrine (adrenalin) showed that some pigs

habituated to a novel, nonpainful swimming task where

they were suddenly placed in a pool of water The task

was repeated over a series of days In some of the pigs, the

elevated epinephrine levels returned to normal and in

other individuals, the epinephrine levels remained high

Some of the pigs lost their fear of swimming and others

remained scared Genetic factors may have accounted for

these differences

EFFECT OF PREVIOUS EXPERIENCES

An animal’s previous experiences with handling will

affect how it will react in the future Cattle that had been

accidentally bumped on the head in a squeeze chute were

more reluctant to reenter the chute a month later Sheep

that had been turned upside down in a restraint device

were more reluctant to reenter the facility the following

year compared to sheep that were restrained in an upright

position.[8]

It is important that an animal’s first experience with a

new person or new place be a good one Progressive

ranchers walk cows and calves through the corrals prior to

doing procedures so that they will associate corrals with

being fed Sometimes painful procedures have to be done,

but it is recommended that they not be associated with the

animal’s first experience with either a new person or a

new place A rat experiment indicated that if a rat was

shocked severely the first time it entered a new arm on a

maze, it would never enter that arm again However, if the

rat was fed the first time he went into the new arm and

then subjected to gradually increasing shocks, he would

keep entering the arm to get the food.[9]

FEAR MEMORIES

If an animal is subjected to either a frightening or a painful

experience, it may form a permanent fear memory that

cannot be erased.[3] This memory is formed in the lower

subcortical pathway in the brain, and extinguishing the

conditioned fear is difficult because it has to be pressed by an active learning process that requires inputfrom higher parts of the cortex The fear memory issuppressed by the cortex, but it can sometimes reappear.Careful, quiet handling of animals will help prevent theformation of fear memories that may compromise welfare,lower productivity, or cause behavior problems, as inhorses Animals can associate certain types of clothing or

sup-a person’s voice with either sup-a frightening or sup-a psup-ainfulexperience Animals also have the ability to recognize thevoice of a familiar safe person who can calm them down

FEAR OF NOVELTYNew experiences and new things are both scary andattractive to animals They are attractive when the animal

is allowed to voluntarily approach, but frightening whensuddenly introduced.[7]If a flag is placed in the middle of

a large field, cattle and horses will approach it andinvestigate However, if the same flag is suddenly wavednext to a horse, he may become highly agitated.[7]Animals can be trained to tolerate new things if theyare gradually introduced Cattle should become accus-tomed to being handled and fed by different people indifferent vehicles This will help reduce stress when theyare moved to a new place Training animals to toleratenew experiences will help keep them calmer It isimportant to train cattle on being moved by both people

on foot and people on horses Cattle appear to perceive aperson riding a horse and a person walking on foot as twodifferent things

TRAIN FOR HANDLINGTraining calves and pigs to handling procedures helps toproduce calmer adult animals Pigs differentiate between aperson in the aisle and a person in their pens Pigs willmove more easily in and out of trucks and through chutes

at a meat plant if the producer trained them by walkingthrough their pens several times each week

Animals will have the lowest amount of fear stresswhen they voluntarily cooperate with being restrained andhandled Zoos and aquariums are training animals, such asapes, lions, and dolphins, to cooperate with blood testingand veterinary procedures Highly excitable Bongoantelope were trained to enter a box and allow bloodsamples to be taken when they were fed treats Almostbaseline cortisol (stress hormone) levels were obtained.The levels of glucose in the blood of trained animals wassignificantly lower compared to the same animal immo-bilized with a dart.[10]

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Reducing fear during handling will improve animal

productivity.[1] There are many different stressors that

animals encounter such as stimuli that evoke fear, heat

stress, cold stress, pain, or fatigue Fear is a strong stressor

and it is one stressor that is easy to reduce Fearful animals

have lower productivity Animals remember frightening

or painful events and producers should be careful to avoid

creation of fear memories An animal’s first experience

with a new corral or person should be low stress Training

animals to handling procedures will help reduce fear

stress Both animal welfare and productivity will be

improved by reducing fear stress

ARTICLE OF FURTHER INTEREST

Animal Handling-Behavior, p 22

REFERENCES

1 Hemsworth, P.H.; Coleman, G.J.; Barnett, J.C.; Berg, S.;

Dowling, S The effect of cognitive behavioral interven

tions on the attitude and behavior of stock persons and

the behavior and productivity of commercial dairy cows

J Anim Sci 2002, 80, 68 78

2 Voisinet, B.D.; Grandin, T.; Tatum, J.D.; O’Connor, S.F.;Struthers, J.J Feedlot cattle with calm temperamentshave higher daily weight gains than cattle with excitabletemperaments J Anim Sci 75, 892 896

3 LeDoux, J The Emotional Brain; Simon and Schuster:New York, New York, 1996

4 Grandin, T Assessment of stress during handling andtransport J Anim Sci 1997, 75, 249 257

5 Lay, D.C.; Friend, T.H.; Bowers, C.C.; Grissom, K.K.;Jenkins, O.C A comparative physiological and behavioralstudy of freeze and hot iron branding using dairy cows

J Anim Sci 1992, 70, 1121 1125

6 Hastings, B.E.; Abott, D.E.; George, L.M.; Staler, S.G.Stress Factors influencing plasma cortisol levels andadrenal weights in Chinese water deer Res Vet Sci

1992, 53, 375 380

7 Grandin, T.; Deesing, M.J Behavioral Genetics andAnimal Science In Genetics and the Behavior of DomesticAnimals; Grandin, T., Ed.; Academic Press: San Diego,

CA, 1998; 1 30

8 Hutson, G.D The influence of barley food rewards onsheep movement through a handling system Appl Anim.Behav Sci 1985, 14, 263 273

9 Miller, N.E Learning resistance to pain and fear, effects ofover learning exposure and rewarded exposure in context

J Exp Psych 1960, 60, 137 142

10 Phillips, M.; Grandin, T.; Graffam, W.; Irlbeck, N.A.;Cambre, R.C Crate conditioning of Bongo (Tragelaptous eurycerus) for veterinary and husbandry procedures at Denver Zoological Garden Zoo Bio 1998, 17,

25 32

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Well-Being Assessment: Behavioral Indicators

J C Swanson

M Rassette

Kansas State University, Manhattan, Kansas, U.S.A

INTRODUCTION

Animal well-being can be characterized as the harmony an

animal is experiencing mentally and physically with its

environment Animal well-being is often used

inter-changeably with the term animal welfare Domestic

livestock and poultry are raised under a variety of

environmental conditions that are vastly different from

those of their wild ancestors The scientific assessment of

the well-being of livestock and poultry has become

important to the sustainability of raising them for food

The best scientific approach and criteria to assess animal

well-being have yet to achieve a scientific consensus, but

it is generally accepted that behavior, physiology, health,

productivity, cognition, and system ecology are indicators

of animal well-being

BEHAVIORAL INDICATORS

The repertoire of behavior expressed by a domestic animal

reflects a living history of its natural and artificial

selection Generally, behavior is used to identify and

assess animal needs, preferences, state of health, ability to

adapt and cope with its social and physical environment,

emotional state, and to gain insight into what an animal

may comprehend or feel about its environment

Several behavioral indicators are commonly cited as

useful to understanding and assessing animal well-being

including abnormal behavior, posture, vocalization,

re-sponsiveness, grooming and displacement behavior,

pre-ferences animals express toward features of their living

environment, and the presence/absence of stereotypies

Abnormal Behavior

The use of abnormal behavior as an indicator of

well-being requires a clear knowledge of what constitutes

normal behavior for a species Species behavior is

se-quenced, measured, described, and recorded to construct

an ethogram The ethogram characterizes both instinctive

and learned behavior displayed throughout a species’ life

cycle Ethograms of wild ancestors, close relatives, or

feral members of the same species are useful in studying

the behavioral similarities and differences induced bydomestication An example of abnormal behavior is anoutbreak of tail biting in pigs The interpretation ofbehavior elicited under domestic conditions is complicat-

ed and requires that we understand the cause, mental aspects, and function of the behavior within theconstruct of the evolutionary and domestic history ofthe species

develop-PostureThe posture of an animal represents a coping response to astimulus Posture is often coupled with other behavioralindicators such as vocalization and locomotion to assesswell-being Researchers have studied the usefulness ofposture to correctly assess the amount of pain and distress

an animal may experience after being subjected tocommon animal management procedures For example,

a behavioral method using posture was validated to assessacute pain associated with different castration proceduresused on lambs.[1]Each procedure was ranked according to

an established index of expected pain Physiologic andbehavioral data (including posture) were then collected for

a period of 60 minutes postprocedure The data wereanalyzed according to the ability to place a lamb into thecorrect procedure group A combination of behavior andposture data correctly placed 79% of the lambs into theirrespective treatment groups.[1]

As technology advances, so too does the sophistication

of using an animal’s posture or movement for assessingwell-being For example, computer image analysis hasbeen used to measure the severity of head movements ofcattle undergoing various types of branding to measuretheir aversion to the procedure,[2] and to evaluate thethermal comfort of pigs based on their proximity to oneanother.[3]While assessments must be validated for otherspecies and for different types of practices, posturalmeasures appear to be useful behavioral indicators ofwell-being

VocalizationAnimals convey a range of emotional states throughvarious types of vocalizations Vocalizations are context-

DOI: 10.1081/E EAS 120019844 Copyright D 2005 by Marcel Dekker, Inc All rights reserved.

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specific, and the circumstances under which vocalizations

are emitted must be carefully considered For example, a

recent study compared the vocalizing of cattle in slaughter

plants before and after modifications were made in animal

handling procedures.[4]The data were used to evaluate the

effectiveness of the plant modifications Indeed, a

reduction in observable aversive events (prod use,

slippage, excessive restraint pressure) decreased the

amount of vocalization behavior.[4] Other researchers

have found similar uses for vocalization in different

species One study measured the occurrence and

frequen-cy of calls in piglets being castrated, and found a

significantly greater rate of high-frequency calls (> 1000

Hz) compared with controls who were handled similarly

but not actually castrated.[5]The researchers were able to

isolate the most painful part of the procedure itself, and

the effect these vocalizations had on other piglets, both of

which have important implications for well-being

Responsiveness

The degree of an animal’s responsiveness to stimuli also

acts as an indicator of well-being For example, the

attitudes and behavior of dairy stockpersons toward

cows have been researched and a correlation found

between the stockperson’s behaviors and the avoidance

distance of cows.[6] Avoidance behavior can shed light

on an animal’s past relationship with humans and reflect

the well-being of individuals or groups Another

example of responsiveness as an indicator of well-being

comes from a study using tonic immobility.[7] Tonic

immobility is a state of petrification induced by

positioning a bird on its back or side consequently,

no movement is detected for a given period of time The

time until the bird recovers head movement, stands, and

walks is measured Shorter latencies to recovery indicate

a better coping response by the bird Reduced or absent

responsiveness of an animal has been recognized as an

indicator of poor well-being

Grooming and Displacement Behaviors

Grooming as a social and self-maintenance behavior

can reflect the relative well-being of an individual or an

entire group Disruption or abnormal manifestations of

grooming are measurable events The lack of grooming,

indicated by poor hair/fur coat or feather condition, is often

used as an indicator of sickness or depression for

indi-vidual animals Abnormal pulling of hair/fur or feathers

or obsessive grooming activities may occur in

individ-uals or within groups Both are considered abnormal

A displacement behavior is the result of frustration or

behavioral disinhibition, or is performed when an animal

is in conflict with how to behave in a given set of

circumstances For example, abnormal feather pecking inlaying hens may be the displaced behavior of naturalforaging or dustbathing and has been used to assessdifferent housing conditions of egg-laying hens.[8]Featherpecking in hens can lead to significant feather loss or evenskin damage Thus, the occurrence of displacementbehavior and abnormal forms of grooming can bemeasured and used to assess well-being

PreferencesPreference tests are valuable tools to evaluate stimuli orconditions by appealing to the desires of the animal Forexample, such tests can be used to assess the effects onwell-being of different enrichment devices or housingconditions In one study, researchers tested the prefer-ences of dairy cattle for different kinds of flooring sand,straw, or a soft rubber mat.[9]The cows avoided sand andpreferred either the mat or straw The researchers thentested whether a preference existed between the mat andstraw They found that cattle preferred straw in winter, but

in summer, cows showed no special preference for onesystem over the other Preference testing of this typeallows for better design of housing systems However,extreme care must be taken when designing and drawingconclusions from such tests For example, exposure toresource cues can affect the performance of an animal inpreference tests.[10] Cues such as odors can be undetect-able to humans, but obvious to animals Carefullycontrolled preference tests are useful in validating theneeds and choices of animals

StereotypiesStereotypy is a common abnormal behavior observed inintensively farmed species and thought to be the product

of impoverished environments Stereotypies are behaviorpatterns repeated without variation and appear to have noobvious goal or function Examples include bar-biting;fur, hair, or wool chewing; sham chewing; tongue lolling;and a variety of locomotion patterns such as head-weaving Once developed, stereotypies can be difficult toextinguish, even when animals are moved into moreenriched environments This indicates an addictivequality to the behavior that requires an understanding ofits neurophysiological development Performance ofstereotypic behavior is often cited as an indicator of poorwell-being

Researchers have studied stereotypies in nearly allfarmed species, including those farmed for fur, such asmink raised in cages.[11] Potential remedies such asenvironmental enrichment are often explored to providerelief However, the view that all stereotypies indicatepoor well-being is controversial.[12–14] Performance of

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stereotypy could also indicate excitement or anticipation

of a resource Thus, stereotypic behaviors are complex

and must be fully examined to determine the effect on

well-being

Although the motivation to stereotype in domestic

species has been researched, the neurophysiological

implications are only beginning to be elucidated For

example, recent studies have linked altered brain

func-tioning and enhanced frustration to stereotypies found in

caged birds.[15]Greater understanding of the disruption to

brain function could eventually adjudicate the competing

views on stereotypic behavior At present, the exhibition

of stereotypies in domestic animals should prompt a closer

look at other well-being indicators to further assess the

possibility of a poor state of well-being

CONCLUSION

Behavior is one of several indicators used to assess animal

well-being There is still much to be learned about the

behavior of our domestic livestock and poultry and what

constitutes a state of good well-being or contentment

Although scientific consensus has not been reached

regarding good versus poor well-being, there is general

agreement that behavior provides insight into factors that

promote or detract from an animal’s quality of life

REFERENCES

1 Molony, V.; Kent, J.E.; McKendrick, I.J Validation of a

method for assessment of an acute pain in lambs Appl

Anim Behav Sci 2002, 76 (3), 215 238

2 Schwartzkopf Genswein, K.S.; Stookey, J.M.; Crowe,

T.G.; Genswein, B.M Comparison of image analysis,

exertion force, and behavior measurements for use in the

assessment of beef cattle responses to hot iron and freeze

branding J Anim Sci 1998, 76 (4), 972 979

3 Xin, H Assessing swine thermal comfort by image

analysis of postural behaviors J Anim Sci 1998, 77(supplement 2), 1 9

4 Grandin, T Cattle vocalizations are associated withhandling and equipment problems at beef slaughter plants.Appl Anim Behav Sci 2001, 71 (3), 191 201

5 Weary, D.M.; Braithwaite, L.A.; Fraser, D Vocal response

to pain in piglets Appl Anim Behav Sci 1998, 56 (2 4),

7 Hocking, P.M.; Maxwell, M.H.; Robertson, G.W.;Mitchell, M.A Welfare assessment of broiler breedersthat are food restricted after peak rate of lay BritishPoultry Science 2002, 43 (1), 5 15

8 El Lethey, H.; Aerni, V.; Jungi, T.W.; Wechsler, B Stressand feather pecking in laying hens in relation to housingconditions British Poultry Science 2000, 41 (1), 2228

9 Manninen, E.; de Passille´, A.M.; Rushen, J.; Norring, M.;Saloniemi, H Preferences of dairy cows kept in unheatedbuildings for different kinds of flooring Appl Anim.Behav Sci 2002, 75 (4), 281 292

10 Warburton; Mason, G.J Is out of sight out of mind? Theeffects of resources cues on motivation in mink Anim.Behav 2003, 65 (4), 755 762

11 Nimon, A.J.; Broom, D.M The welfare of farmed mink(Mustela vison) in relation to housing and management: Areview Animal Welfare 1999, 8 (3), 205 228

12 Vinke, C.M Some comments on the review of nimon andbroom on the welfare of farmed mink Animal Welfare

15 Garner, J.P.; Mason, G.J.; Smith, R Stereotypic routetracing in experimentally caged songbirds correlates withgeneral behavioural disinhibition Anim Behav 2003, 66(4), 711 727

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