Beauveria bassiana
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Sustainable insect management:
Experiences in development
of a Canadian biopesticide
Dan Johnson
of a Canadian biopesticide
(Metarhizium anisopliae)
Professor of Environmental Science,
Canada Research Chair in Sustainable Grassland Ecosystems
Water and Environmental Science Building,
Department of Geography,
University of Lethbridge, Alberta, Canada
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dan.johnson@uleth.ca
Water and Environmental Science Building
University of Lethbridge, Alberta, Canada.
We are currently working toward licensing, in
cooperation with Office of Research Services, and
Health Canada PMRA
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Metarhizium anisopliae (photos D Johnson, 2010)
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dan.johnson@uleth.ca
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80 100
dan.johnson@uleth.ca
20 40 60 80
IMI 330189 S54
Cumulative mortality of field-collected grasshoppers treated
with Metarhizium anisopliae var acridum IMI 330189 and M
anisopliae var anisopliae S54
0
1 2 3 4 5 6 7 8 9
Days
anisopliae var anisopliae S54
Entz, S.C., Kawchuk, L.M and Johnson, D.L 2008 Discovery of a North American
genetic variant of the entomopathogenic fungus Metarhizium anisopliae var anisopliae
pathogenic to grasshoppers BioControl 53(2): 327-339.
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Production on grain: optimizing temperature, nutrients and cycle time
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We adapted our application technology to the applications
methods currently in use by land managers and farmers
dan.johnson@uleth.ca
methods currently in use by land managers and farmers.
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Metarhizium anisopliae
Plate sample from field during spray application
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0.25 sq m
sampling ring
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Trichomalopsis
Trichomalopsis
sarcophagae
(received from Dr K Floate)
Safe for candidate biocontrol agents of biocontrol agents of nearby agricultural systems
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90
100
50
60
70
80
10^4 s pore
20
30
40
50
10^6s pore 10^7s pore
Trichomalopsis sarcophagae
0
10
D ay
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Replicated experiments showed
no impact on rainbow trout
dan.johnson@uleth.ca
no impact on rainbow trout,
crayfish or earthworms
More detailed histopathology
tests are in progress
In cooperation with Lethbridge
In cooperation with Lethbridge College Aquaculture Center of Excellence
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Chaoborus americanus
dan.johnson@uleth.ca
0, 1.5x104, 3.0x104, or 1.2x105 fungal spores per ml, of
Metarhizium anisopliae var
anisopliae isolate S54
Negligible mortalities in Negligible mortalities in replicated treated or control jars
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Funding and cooperation:
Funding and cooperation:
AAFC Pesticide Risk Reduction Program, Pest Management Centre
Alberta Crop Industry Development Fund
Canada Foundation for Innovation
Canada Research Chairs
Department of National Defence, Canada (environmentally sustainable
Department of National Defence, Canada (environmentally sustainable
airfield insect control to reduce birdstrikes)
NSERC
Pulse Canada
Saskatchewan Pulse Growers