Molecular Biotechnology-Lession 1 pptx

and Pasternak, J.J 2003 Molecular Biotechnology: Principles & Applications of Recombinant DNA, 3rd ed..  Assignment An article from journals relating to biotechnology o Objective of t

Molecular Biotechnology

Tran Ngoc Duc, PhD

VIETNAM NATIONAL UNIVERSITY AT HO CHI MINH CITY

INTERNATIONAL UNIVERSITY

 Time and location

 Glick, B.R and Pasternak, J.J (2003) Molecular Biotechnology: Principles &

Applications of Recombinant DNA, 3rd ed ASM Press: Washington, D.C.

 the presentation should be submitted latest at 5PM

on Sunday afternoon 50pt will be lost if sending late

 Sign in 2 times: beginning and end 15 min late => no enter + 3pt off, leaving after break=> 3pt off

 Absent for 2 classes=> no exam

 Assignment

 An article from journals relating to biotechnology

o Objective of the article

o Materials, methods are used

 Vaccines production, edible vaccines

 Insect, pathogen, herbicide resistant

Course prerequisite (supporting molecular biotechnology)

 Part I: Introduction to molecular biotechnology (1class)

 Part II: Basic techniques in DNA technology (2 classes)

 Part III: Recombinant DNA technology (3 classes)

 Part IV: Gene expression in biological systems (4 classes)

 Part V: Regulatory and ethical issues (1 class)

Part I Introduction to molecular biotechnology

 Biotechnology

 Traditional biotechnology

 Modern/molecular biotechnology

 Prokaryotic and eukaryotic systems

 Journals of biotechnology (open and security): go to Brooklyn college

website

Various names of biotechnology journals

Examples of biotechnological products?

 An applied field of biology that involves the use of living organisms and bioprocesses in engineering, technology, medicine and other fields requiring

 Traditional Biotechnology

Traditional biotechnology refers to a number of ancient ways of

using living organisms to make new products or modify

existing ones In its broadest definition, traditional

biotechnology can be traced back to human's transition from

hunter-gatherer to farmer As farmers, humans collected wild plants and cultivated them and the best yielding strains were selected for growing the following seasons

Read more:

http://wiki.answers.com/Q/What_is_the_differentiate_traditional_biotechnology_by_the_modern_biotechnology#ixzz1XqJWP8NU

 From hunter to farmer

 Selective breeding, hybridization and mutagenesis

 Selection of superior or desirable plants for food

 Domestication of plants, animals

 Use plant for fuel, perfume, oil, medicine, dye

 Using microorganism and organisms in agriculture and industry

 Waste treatment

 More

 So…

 Limit of traditional biotechnology

Professor Ian Wilmut

 Modern Biotechnology

Modern biotechnology refers to a number of techniques that

involve the intentional manipulation of genes, cells and living

tissue in a predictable and controlled manner to generate

changes in the genetic make-up of an organism or produce new tissue Examples of these techniques include: recombinant DNA techniques (r DNA or genetic engineering), tissue culture and mutagenesis

Read more:

(http://wiki.answers.com/Q/What_is_the_differentiate_traditional_biotechnology_by_the_modern_biotechnology#ixzz1XqKRtLNY)

It’s molecular biotechnology, which is contributed by many scientific disciplines and generates a wide range of commercial products

 Modern biotechnology (cont.)

 The invention of DNA structure by Watson and Crick in 1950s, leading to the emergence of recombinant DNA

technology in late 1970s

 Recombinant DNA = gene cloning = genetic engineering

 Create rather than isolate strains, and improve larger amount of products

 Prokaryotic and eukaryotic organism systems

 Biological systems: bacteria, fungi, insect, plant,

mammalian cell lines, virus, animals

 Baterium Escherichia coli and yeast Saccharomyces

systems

 Presence and absence of a nucleus

 Presence and absence of sub-cellular cytoplasmic organelles

 Chemical components of the cell wall (peptidoglycan, chitin, cellulose)

 Single celled yeast (5µm)

 Convert sugar into alcohol and c02

 Model organism, 1st genome sequenced in 1996

 Applications of recombinant biotechnology

 Recombinant human insulin

 Recombinant human growth hormone

 Recombinant human blood clotting factor III

 Recombinant hepatitis B vaccine

 Carotenoids production

 Herbicide resistant crop

 Insecticide resistant crop

 Glofish is the first transgenic animal approved to be

consumed by

human in the USA

 The insertion of different constructs

of GFP into the

fish genomes to give different

green colors

End of Part I