Pham Van Hung, PhD
What on Earth did scientist do before Chromatography?
What entices the scientists to Chromatography?
| Brief History of Chromatography
| History of the Main techniques
Trang 2
Chromatography
| Principles of Chromatography
Bante of Chromatography
| Paper Chromatography and Thin Layer Chromatography (TLC) |
| Column Chromatography |
| Gas Liquid Chromatography (GLC) |
| High Performance Liquid Chromatography (HPLC) |
| Chromatography
| Thin-layer chromatography
Principles of TLC
Trang 3
TLC
Normal Phase
Reversed Phase
Adsorbents for TLC
Steps in TLC Analysis
Thin Layer (and Paper) Chromatography
I
Trang 4
Thin Layer (and Paper) Chromatography
Thin Layer and Paper Chromatòraphy
&) N4 @ a a
Column Chromatography
Column Chromatography
+ + * 4
HPLC Introduction:
l8
1903: Russian botanist Mikhail Tswett
Trang 5
'HPLC History
Early 1950s: First appearance of GC
1964: J Calvin Giddings
lì n9
1966: Horvath
1970's: HPLC became popular with an increase in technology
Overview of the HPLC Process
- Diagram of HPLC Apparatus:
- Regulated
1 , helium source ——>
à
Os
KP
NA
Happy time now?