An intronic RUNXI enhancer marks hematopoietic stem cells

... Generation and analyses of Runx1 +24.1 enhancer transgenic mice124 4.5.1 Runx1 +24.1 enhancer has hematopoietic Runx1-specific enhancer activity in the mouse embryo 128 4.5.1.1 Runx1 +24.1 enhancer ... OP9 feeder cells 135  xiv Figure 4.14: Runx1 +24.1 enhancer activity marks majority of KSL HSC/progenitor cells and only few lineage-positive BM cells 138  Figure 4.15: Runx...

Ngày tải lên: 11/09/2015, 09:10

... peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic ... whether a commercially-available nutraceutical, Stem- Kine (Aidan Products, Chandler AZ), was capable of increasing the number of circulating stem cells...

Ngày tải lên: 18/06/2014, 16:20

... doi:10.1186/1479-5876-9-96 Cite this article as: Ponnikorn et al.: Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia Journal of Translational Medicine 2011 9:96 Submit your ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid...

Ngày tải lên: 18/06/2014, 19:20

... much of what we know about stem cells is imprecise and based on inferences from genetically manipulated animals Cardinal Functions of Hematopoietic Stem Cells All stem cell types have two cardinal ... molecular changes accompanying functional changes in maturing cells, aggregating cells into functional subgroups, and demonstrating hematopoietic stem cell regulation by a spe...

Ngày tải lên: 07/07/2014, 01:20

... The presence of stem cells in the circulation is not unique to a time window in development Rather, hematopoietic stem cells appear to circulate throughout life The time that cells spend freely ... or a different host Mobility of Hematopoietic Stem Cells Cells entering and exiting the bone marrow so through a series of molecular interactions Circulating stem cells (throu...

Ngày tải lên: 07/07/2014, 01:20

... progenitor proliferation Hematopoietic stem cells have governing mechanisms that are distinct from the cells they generate Hematopoietic Stem Cell Differentiation Hematopoietic stem cells sit at the ... location of stem cells by indirect means, altering molecules such as CXCL12 that tether stem cells to their niche Molecules shown to be important for altering the pro...

Ngày tải lên: 07/07/2014, 01:20

... capacity, stem cells have an additional feature characterizing their proliferation machinery Stem cells in most mature adult tissues are deeply quiescent In the hematopoietic system, stem cells are ... important function of stem cells because it is critical in regulating the number of stem cells Stem cell number is a key limiting parameter for both autologous and allog...

Ngày tải lên: 07/07/2014, 01:20

... Else Can Hematopoietic Stem Cells Do? Some experimental data have suggested that hematopoietic stem cells or other cells mobilized into the circulation by the same factors that mobilize hematopoietic ... experimentally in the hematopoietic system Taking advantage of the cell-surface markers that distinguish hematopoietic cells of varying maturity, stem cells, progenit...

Ngày tải lên: 07/07/2014, 01:20

... regulatory pathways used by normal and malignant stem cells Emerging evidence suggests that both normal and cancer stem cells share common developmental pathways Since the signaling pathways that ... University, Syracuse, NY, 13210, USA 2Center for Humanized SCID Mice and Stem Cell Processing Laboratory, SUNY Upstate Medical University, Syracuse, NY, 13210, USA Authors’ contri...

Ngày tải lên: 12/08/2014, 23:23

... Komarova et al Journal of Ovarian Research 2010, 3:12 http://www.ovarianresearch.com/content/3/1/12 a means to track malignant tissues or for the delivery of anticancer agents to tumors [2,5-9] Several ... relevance of our strategy in an ovarian tumor model, and showed that the number of modified MSC carriers increased in intraperitoneal tumors Artificial receptor stra...

Ngày tải lên: 20/06/2014, 07:20

... within a small number of cells in monolayer culture Following injection into NP tissue explants, MSCs stained strongly positive for type II collagen and matrix staining in the vicinity of the injected ... viability/metabolic activity of injected mesenchymal stem cells Photomicrographs representative of caspase immunopositivity and polyAcells mRNA staining in...

Ngày tải lên: 09/08/2014, 01:22

... successfully in case reports and small series, with apparently low acute toxicity [57,63] Long-term safety data are needed Scanty and sometimes contradictory AD animal data are available Although the results ... engineer cartilage, bone, muscle, fat, tendon and neuronal cells; they may serve as cell vehicles for gene therapy; they may enhance engraftment in haematopoietic stem cell transp...

Ngày tải lên: 09/08/2014, 08:23

... of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung An FFPE proteomic study Journal of Clinical Bioinformatics 2011 1:23 Submit your next manuscript to ... upon Tyne, U.K.), polyclonal rabbit anti CGA antibody (DAKO Japan, Kyoto, Japan) and monoclonal mouse anti SYN antibody (DAKO Japan, Kyoto, Japan) The staining...

Ngày tải lên: 10/08/2014, 09:22

... doi:10.1186/1756-8722-4-38 Cite this article as: Yang et al.: Enhanced self-renewal of hematopoietic stem/ progenitor cells mediated by the stem cell gene Sall4 Journal of Hematology & Oncology 2011 4:38 • Inclusion in ... Further more, these Ly5.2+ cells were able to reconstitute granulocyte, T cells, and B cells, as well as immature blood cells, as indicated...

Ngày tải lên: 10/08/2014, 21:23