hematopoietic stem cells differentiation ppt

... Leukemic Stem Cells Three hypotheses have been proposed that lead to the development of leukemic stem cells (LSC/CSC): (A) LSC/CSC might arise from either a hematopoietic stem cell (HSC), hematopoietic ... pluripotency and by actively inducing lineage commit-ment and differentiation of HSCs [9-18] Leukemia Stem Cells/Cancer Stem Cells (LSC/CSC) The cancer stem cell hypothesis postulates that cancer can be ... variety of cell types, including hematopoietic and stromal cells of the bone marrow, endothelial cells, epithelial cells, fibroblasts, neuronal cells, and smooth muscle cells [3,53-57] The bone marrow

Ngày tải lên: 12/08/2014, 23:23

... centers on hematopoietic stem cells (HSCs), which are essential blood stem cells located in adult bone marrow These cells play a crucial role in regenerating blood and immune system cells Investigations ... system disorders.The different classes of hematopoietic stem cells can be categorized by their potency and structured in a tree [42,45] (see Figure 1-1) At the root of the hierarchy are the Hematopoietic ... One area in medicine that shows considerable potential is stem cell research.High-throughput time-lapse microscopy of murine stem cellsStem cell research offers significant potential for developing

Ngày tải lên: 10/05/2022, 14:58

... Level of Hematopoietic Stem Cells: Inference from Differentiation in Daughter Cell and Granddaughter Cell Pairs J Exp Med 199, 295–302 (2004). 6 Ema, H et al Adult mouse hematopoietic stem cells: ... of hematopoietic cells (Gr1+ Mac1+ monocytes, B220+ B cells, CD3+ T cells) (Fig. 2E, Fig. S3B) These results implied stable integration of the GFP reporter gene into neonatal hematopoietic stem/progenitor ... adult hematopoietic stem/progenitor cells Having confirmed the in vivo transduction capacity of the Vs-envA/GFP lentivirus to genetically label neonatal hematopoietic stem/progenitor cells,

Ngày tải lên: 19/11/2022, 11:40

... and MPP cells in BM among donor-derived Ly 5.1 + LSK cells in young and old recipient (Ly 5.2 + ) mice. D Frequency of young B cells, T cells and myeloid cells among donor-derived Ly 5.1 + cells ... for early hematopoietic progenitor cells (LSK) cells in OPN / animals (Nilsson et al, 2005) Y?O animals presented with an increase in the frequency of myeloid cells over lymphoid T cells, while ... 2016 | Accepted 18 January 2017 Introduction One cause of aging of the hematopoietic system is aging of hematopoietic stem cells (HSCs) While upon aging the number of phenotypic HSCs increases,

Ngày tải lên: 04/12/2022, 15:58

... investigated its role in the response of hematopoietic stem cells (HSC) to liver fibrosis in mice Methods: Purified (c-kit+/sca1+/lin-) hematopoietic stem cells (HSC) were repeatedly infused into ... therapeutic paradigm for the use of hematopoietic stem cells in this setting It has previously been proposed that injected bone marrow cells differentiate into anti-fibrotic cells of a monocyte/macrophage ... Identification and isolation of hematopoietic stem cells Arch Med Res 2003;34:461-75 20 Massberg S, Schaerli P, Knezevic-Maramica I, et al Immunosurveillance by hematopoietic progenitor cells trafficking through

Ngày tải lên: 26/07/2023, 07:40

... pluripotent progenitor/stem cells; their prog-eny includes chondrocytes, tendon cells, haematopoiesis-sup-port stromal cells, adipocytes and osteoblasts [3,4] MSCs, similar to other stem cells, have an ... during chondrogenic differentiation of mesenchymal stem cells (MSCs) Time-course expression of chemokines and their receptors during chondrogenic differentiation of mesenchymal stem cells (MSCs) The ... during chondrogenic differentiation of mesenchymal stem cells (MSCs) Expression levels of ADAM and adhesion molecules during chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene-expression

Ngày tải lên: 09/08/2014, 10:20

... Other types of stem cells Hematopoietic stem cells together with mesenchymal stem cells, the so-called“side population”, and multipo-tent adult progenitor cells (MAPCs), are the stem cells forming ... induced pluripo-tent stem cells are at least, if not more capable than those from adult (stem) cells Mesenchymal stem cells Although adult stem cells are primarily unipotent cells, under certain ... Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cells of a patient through

Ngày tải lên: 18/06/2014, 16:20

... due to the neural differentiation of implanted stem cells. Keywords: adipose-derived stem cells, dog, spinal cord injury, transplantation Introduction Central nervous system regeneration is ... because fibroblast cells also behave in this manner Some critics have suggested that even a low fraction of contaminating cells such as hematopoietic stem cells could be the source of differentiation ... has been performed [4] to remove CD45+ cells (leucocytic/ hematopoietic lineage) and CD31+ cells (endothelial lineage) from the isolated cells prior to differentiation experiments Given the relative

Ngày tải lên: 07/08/2014, 23:22

... lineage-specific differentiation of mesenchymal stem cells In addition we review recent research on the concept of stem cell niche, and its relevance to adult mesenchymal stem cells Introduction ... expansion of stem cells, increased in vivo reconstitution of hematopoietic lineages, and Wnt3a-specific maintenance of skin and intestinal stem cell populations [18] Because stem cells may share ... of all hematopoietic cells) CD34, the primitive hematopoietic stem cell (HSC) marker, is rarely expressed in human MSCs, although it is positive in mice CD31 (expressed on endothelial and hematopoietic

Ngày tải lên: 09/08/2014, 10:20

... label viable cells This combination allows the differen-tiation among early apoptotic cells (Ann5 positive, 6-CFDA positive), necrotic cells (Ann5 positive, 6-CFDA negative), and viable cells (Ann5 ... Tris-buffered saline; TGF: transforming growth factor. Trang 2Mesenchymal progenitor cells, also referred to as mesenchy-mal stem cells (MSCs), provide an attractive alternative to chondrocytes with regard ... induce chondrogenic differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondro-progenitor cells [1,7-11] During

Ngày tải lên: 09/08/2014, 14:22

... multipotent mesenchymal stem cells (MSCs) offer renewable cells for generating insulin-producing cells (IPCs) Methods: We used a four-stage differentiation protocol, containing neuronal differentiation ... SC, Cheng H, Pan CY, Tsai MJ, Kao LS, Ma HL: In vitro differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529. 3 Tzeng SF, Tsai MJ, Hung ... cell death and differentiation of stem cells or progenitor cells Pancreatic ECM mainly consists of fibronectin (FN) and laminin (LAM) Pancreatic FN is noted beneath the endothelial cells and epithelial

Ngày tải lên: 10/08/2014, 05:21

... co-cultured CD34+ hematopoietic stem cells with 3A6 cells, we noted the formation of cobblestone areas from hematopoietic cells that transmigrated beneath the layer of 3A6 cells (Figure 3C) Previously, ... is associated with differentia-tion of neural stem cells, hematopoietic stem cells and MSCs However, an increase in Oct4 expression in ESCs causes differentiation into primitive endoderm [29], ... and differentiation associated genes Principal component analysis further pointed out the expression profile of the cells converged toward embryonic stem cells These data demonstrate these cells

Ngày tải lên: 10/08/2014, 05:21

... hepatic stem cells These include hematopoietic and mesenchymal stem cellsderived from the bone marrow, peripheral blood or umbilical cord blood, and extra-embryonic stem (ES) cells (18) Whether hematopoietic ... liver cells from donor hematopoietic cells (29–34) On the other hand, hematopoietic stem cells did not show the capacity to generate oval cells (35) Also, the overall efficiency by which hematopoietic ... human hematopoietic stem cells transplanted into mice (39), so the overall potential of hematopoietic stem cells in liver-directed cell therapy is quiteuncertain Insights into how human ES cells

Ngày tải lên: 10/08/2014, 18:21

... expanding clinically transplantable stem cells Keywords: Mouse Hematopoietic Stem Cell, Transplantation, Differentiation Background Hematopoietic stem cells (HSCs) are rare cells defined by their unique ... Nakajima K, Kanakura Y: Roles for c-Myc in self-renewal of hematopoietic stem cells J Biol Chem 2004, 279:24986-93 27 Nakano T: Hematopoietic stem cells: generation and manipulation Trends Immunol 2003, ... GFP control cells was not detected When the number of infused cells was raised to × 105 cells, both Sall4A and Sall4B transduced cells repopulated of recipients, whereas GFP transduced cells did

Ngày tải lên: 10/08/2014, 21:23

... adipose tissue derived mesenchymal stem cells In order to demonstrate that the cells isolated from the canine adipose tissue (Figure 1A) are indeed mesenchy-mal stem cells (MSCs) we differentiated ... (PARP) [9] During embryonic development, cartilage develops from mesenchymal stem cells (MSCs) by condensation and differentiation Recent studies have shown that MSC-like progenitors also exist ... of cells exhibiting typical morphological features of apoptotic cell death was determined by scoring 100 cells from 30 different microscopic fields per culture and the number of apoptotic cells

Ngày tải lên: 12/08/2014, 14:22

... HIV-1, gp120, mesenchymal stem cells, cell differentiaton, apoptosis Background Although the main targets of HIV infection pathogenesis are the CD4+ cells of the immune system, several stu-dies ... passages these cells exhibited RT-PCR Trang 5positive detection of embryonic stem cell marker Oct-4 aswell as some molecules known to play a role in critical regulatory pathways of stem cells, such ... 8smooth muscle and endothelial cells To study theeffects of HIV-1 on the differentiation of these cells, the interaction of HIV-1 and recombinant gp120 on MSC differentiation to adipogenic and

Ngày tải lên: 13/08/2014, 01:20

... Transcriptome of adult stem cells <p>Comparison of the transcriptomes of pluripotent embryonic stem cells, multipotent adult progenitor cells and lineage restricted mes-enchymal stem cells identified ... ability than classical adult stem cells Here we compare the transcriptome of pluripotent embryonic stem cells (ESCs), MAPCs, and lineage-restricted mesenchymal stem cells (MSCs) to determine their ... progenitor cells.</p> Abstract Background: Recently, several populations of postnatal stem cells, such as multipotent adult progenitor cells (MAPCs), have been described that have broader differentiation

Ngày tải lên: 14/08/2014, 08:20

... human stem cells. Stem Cells Dev 2006, 15(1):118-23. 77. Shytle RD, Ehrhart J, Tan J, Vila J, Cole M, Sanberg CD, Sanberg PR, Bickford PC: Oxidative stress of neural, hematopoietic, and stem cells: protection ... These data suggest Stem- Kine supplementation increases circula- tion of cells capable of giving rise to hematopoietic- lineage cells in vitro. Stem- Kine augments circulation of cells with EPC phenotype Agents ... these cells. Given the possibility that both hematopoietic stem cells and EPC may serve as endogenous “repair cells ,we sought to assess a relatively non-invasive means of mod- ulating these cells. Stem- Kine...

Ngày tải lên: 18/06/2014, 16:20

... induction of apoptosis and the relevant signaling pathways in hematopoietic stem cells are still poorly understood. Hematopoietic stem cells (HSCs) contain specific markers on their surface such ... phosphoproteins in HbE/b-thalassemic stem cells was proposed (Figure 4). Discussion Conventional methods typically have difficulty using fresh human hematopoietic stem cells for studying cell signaling ... culture of hematopoietic stem cells (HSCs) Bone marrow samples (BM) from Hb E/b-thalassemic patients and healthy donors were collected for HSCs iso- lation. Briefly, mononucl ear cells (MNCs)...

Ngày tải lên: 18/06/2014, 19:20