cd34 negative hematopoietic stem cells

... relevant signaling pathways in hematopoietic stem cells are still poorly understood Hematopoietic stem cells (HSCs) contain specific markers on their surface such as CD34 and CD133 allowing for ... erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according to a procedure previously described [13] with some modifications HSCs/CD34 + cells were ... expressed the cell surface marker CD34 and had low levels of CD45 and were negative for glycophorin A The purity of the iso-lated CD34+cells was 92% (data not shown) CD34+cells from patients and donors

Ngày tải lên: 18/06/2014, 19:20

... wherein hematopoietic stem cells (HSCs) differ-entiate into mature hematopoietic cells [1] It is a pro-cess controlled by complex interactions between numerous genetic processes in blood cells ... expression of CD34 and represents a heterogeneous cell population encompassing stem cells, early pluripotent progenitor cells, multipotent progeni-tor cells, and uncommitted differentiating cells [8] ... [9-18] Leukemia Stem Cells/Cancer Stem Cells (LSC/CSC) The cancer stem cell hypothesis postulates that cancer can be initiated, sustained and maintained by a small number of malignant cells that have

Ngày tải lên: 12/08/2014, 23:23

... centers on hematopoietic stem cells (HSCs), which are essential blood stem cells located in adult bone marrow These cells play a crucial role in regenerating blood and immune system cells Investigations ... system disorders.The different classes of hematopoietic stem cells can be categorized by their potency and structured in a tree [42,45] (see Figure 1-1) At the root of the hierarchy are the Hematopoietic ... types.M Kroiss, Predicting the Lineage Choice of Hematopoietic Stem Cells, Using recurrent neural networks (RNNs) to predict the lineage choice of stem cells reveals key trends in gene expression

Ngày tải lên: 10/05/2022, 14:58

... primitive hematopoietic stem cell compartment through the utilization of a modified Rcas/TVA system We succeeded in transferring a GFP reporter gene into adult hematopoietic stem cells in vivo, ... of hematopoietic cells (Gr1+ Mac1+ monocytes, B220+ B cells, CD3+ T cells) (Fig. 2E, Fig. S3B) These results implied stable integration of the GFP reporter gene into neonatal hematopoietic stem/progenitor ... analysis of GFP+ cells in various hematopoietic organs at 24 weeks post-injection CD150+ CD34−/lowKSL represent primitive hematopoietic stem cell fraction (considered a more highly purified stem cell

Ngày tải lên: 19/11/2022, 11:40

... and MPP cells in BM among donor-derived Ly 5.1 + LSK cells in young and old recipient (Ly 5.2 + ) mice. D Frequency of young B cells, T cells and myeloid cells among donor-derived Ly 5.1 + cells ... for early hematopoietic progenitor cells (LSK) cells in OPN / animals (Nilsson et al, 2005) Y?O animals presented with an increase in the frequency of myeloid cells over lymphoid T cells, while ... 2016 | Accepted 18 January 2017 Introduction One cause of aging of the hematopoietic system is aging of hematopoietic stem cells (HSCs) While upon aging the number of phenotypic HSCs increases,

Ngày tải lên: 04/12/2022, 15:58

... investigated its role in the response of hematopoietic stem cells (HSC) to liver fibrosis in mice Methods: Purified (c-kit+/sca1+/lin-) hematopoietic stem cells (HSC) were repeatedly infused into ... therapeutic paradigm for the use of hematopoietic stem cells in this setting It has previously been proposed that injected bone marrow cells differentiate into anti-fibrotic cells of a monocyte/macrophage ... Hepatocyte Growth Factor which recruit human CD34+ progenitors32 We have previously reported that the adhesion of human hematopoietic (CD34+) stem cells to human liver compartments is integrin

Ngày tải lên: 26/07/2023, 07:40

... of CD34ⴙCXCR4ⴙcells, and the frequency of cobblestone area-forming cells was 6 times higher in the CD34ⴙCXCR4ⴚpopulation in comparison to CD34ⴙCXCR4ⴙcells Furthermore, the most quiescent CD34ⴙcells ... that the CD34⫹CXCR4⫺ cells contained 6-fold more CAFC than CD34⫹CXCR4⫹ cells (Figure 6) These findings clearly demonstrate that BM CD34⫹CXCR4⫺ cells are more primitive than CD34⫹CXCR4⫹cells by ... control The sort regions for CD34⫹CXCR4⫹and CD34⫹CXCR4⫺cells are shown in (B). Figure 5. Content of hematopoietic progenitor cells within CD34⫹CXCR4⫹and CD34⫹CXCR4⫺cells obtained from steady-state

Ngày tải lên: 02/11/2022, 10:40

... expanding clinically transplantable stem cells Keywords: Mouse Hematopoietic Stem Cell, Transplantation, Differentiation Background Hematopoietic stem cells (HSCs) are rare cells defined by their unique ... expressed in CD34 positive hematopoietic stem/progenitor cells [16] To further test the effect of Sall4 in LSK cells, we performed a Sall4 knockdown study in isolated mouse BM LSK cells by an ... Nakajima K, Kanakura Y: Roles for c-Myc in self-renewal of hematopoietic stem cells J Biol Chem 2004, 279:24986-93 27 Nakano T: Hematopoietic stem cells: generation and manipulation Trends Immunol 2003,

Ngày tải lên: 10/08/2014, 21:23

... Mononuclear Cells FITC Fluorescein Isothiocyanate HSC Hematopoietic Stem Cells HSPC Hematopoietic Stem and Progenitor Cells Marrow ECM Marrow Extracellular Matrix Trang 8Hematopoietic stem cells ... hematopoietic cells to ALCAM expressing components of the hematopoietic niche via dimerization We investigated the role of ALCAM on the homing abilities of hematopoietic stem and progenitor cells ... associated with enhanced hematopoietic discovered on thymic epithelial cells35, and activated leukocytes6 It is also expressed in most primitive CD34+ hematopoietic cells (rhodamine 123lo, Thy-1+,

Ngày tải lên: 24/08/2014, 09:44

... marrow stromal cells; hHSC, human hematopoietic stem cell Trang 9Fig 4 Mouse model of peripheral blood hematopoietic stem cell mobilization a, b, c The mouse model of hematopoietic stem cell (HSC) ... numbers of total, CD34+, and CD34+/CD45- cells de-termined using a hemocytometer and flow cytometric ana-lysis of CD34+ cells cultured with IL-8 were significantly higher than those of cells cultured ... Human BM CD34+ HSCs were purchased from Lonza (catalog no 2 M-101) and cultured in Stemline® II Hematopoietic Stem Cell Expansion Medium (Sigma-Aldrich, catalog no S0192) containing 100 ng/mL stem

Ngày tải lên: 06/08/2020, 05:25

... with 15 min The hematopoietic progenitor cells (HPCs) (Lin–c-kit+Sca-1−), hematopoietic stem cells (HSCs) (Lin–c-kit+Sca-1+) and long-term hematopoietic stem cells (LT-HSCs) (CD34–Lin–c-kit+Sca-1+) ... mononuclear cells (BMMNCs) and mesenchymal stem cells (MSCs) were established in our preliminary studies8 Iron overload could impair hematopoiesis by damaging hematopoietic cells and the hematopoietic ... myeloid cells are presented as the means ± SE of three independent experiments (d–f) The frequencies of hematopoietic progenitor cells (HPCs) (Lin-c-kit+Sca–1–, LKS–), hematopoietic stem cells

Ngày tải lên: 04/12/2022, 16:13

... lymphocytes [4-6] Since mature blood cells are short-lived, hematopoietic stem cells (HSCs) are required throughout life to replenish progenitor and precursor cells [5, 7] A stem cell niche is a specialized ... Running Title: Targeting leukemic cells in stem cell niches Trang 4ACCEPTED MANUSCRIPTAcute myeloid leukemia and acute lymphoblastic leukemia cells hijack hematopoietic stem cell (HSC) niches in the ... multipotent stem cells with self-renewal capacity that give rise to various types of progenitor cells and ultimately the production of mature erythrocytes, megakaryocytes, myeloid cells, and lymphocytes

Ngày tải lên: 26/07/2023, 07:40

... pluripotent stem cells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, technologies, and especially those related to stem cell-based ... embryonic stem cells: the birth of the fi rst IVF test-tube baby in 1978 (Steptoe and Edwards 1978) and the discovery in 1981 by Martin (1981) and Evans and Kaufman (1981) of mouse embryonic stem cells ... potential For stem cell-based therapies and screening, robust production of cells in controlled dynamic cultures (bioreactors) is required To that end, methods for expansion of pluripotent stem cells

Ngày tải lên: 22/03/2014, 09:20

... Trang 1 Tế bào gốc và ứng dụng trong y sinh học (Stem cells and the application in biomedicine) Nguyễn Thị Thu Hà* Bệnh viện Trung ương Quân đội ... bào thai người (Human Embryonic Stem cell) Năm 1998 nhóm nghiên cứu của James Thomson đã phân lập và duy trì được một loại tế bào gốc bào thai (ES: embryonic stem cell) từ khối tế bào bên trong ... điều hoà quá trình tăng sinh và biệt hoá một cách mềm dẻo như vậy? iv Tế bào gốc tạo máu (Hematopoietic Stem Cell) Cho tới nay tế bào gốc tạo máu đã được nghiên cứu hơn nửa thế kỷ và là một trong

Ngày tải lên: 02/04/2014, 22:20

... ofstem cells found in bone: hematopoietic stemcells which form blood and immune cells, andstromal (mesenchymal) stem cells that normallyform bone, cartilage, and fat The restrictedcapacity of hematopoietic ... and the Promise of Stem Cell-Based Therapies 59 Chapter 7: Stem Cells and Diabetes 67 Chapter 8: Rebuilding the Nervous System with Stem Cells 77 Chapter 9: Can Stem Cells Repair a Damaged ... heart cells, skin cells, or nerve cells THE DIFFERENTIATION POTENTIAL OF STEM CELLS: BASIC CONCEPTS AND DEFINITIONS Many of the terms used to define stem cells depend on the behavior of the cells

Ngày tải lên: 11/04/2014, 10:33

... type 2 (VEGF-R2)-positive cells induced from mouse embryonic stem (ES) cells can differentiate into both endothelial cells (ECs) and mural cells (MCs) and these vascular cells construct blood vessel ... vascular cells differentiated from human ES cells Figure 1 Schematic representation of preparation of the transplanted vascular cells differentiated from human ES cells. human embryonic stem cells ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs),

Ngày tải lên: 18/06/2014, 15:20

... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that ... miRNA upregulated in hES cells, 31 upregulated in EB cells, and 35 upregulated in adult cells (Figure 2) The 20 miRNAs most highly expressed in hES cells, EB, and adult cells respectively were ... supervised clustering (A) Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical

Ngày tải lên: 18/06/2014, 15:20

... had Hematopoietic differentiation of blast cells Figure 2 Hematopoietic differentiation of blast cells Figures A-F show different types of hematopoietic colonies and cells derived from blast cells ... susceptible to immune rejec-tion than adult cells Stem Cells 2006, 24(2):221-9. 30. Koch CA, Geraldes P, Platt JL: Immunosuppression by embry-onic stem cells Stem Cells 2008, 26(1):89-98. 31. Fabricius ... these cells expressed also HLA class I molecules, however at very low levels compared to endothelial and hematopoietic cells The blast cells could be successfully differentiated to hematopoietic cells

Ngày tải lên: 18/06/2014, 15:20

... statistical significance. Results Stem- Kine mobilizes CD34 and CD133 Cells Quantification of peripheral blood cells expressing the hematopoietic stem cell markers CD133 and CD34 was performed at day ... human stem cells. Stem Cells Dev 2006, 15(1):118-23. 77. Shytle RD, Ehrhart J, Tan J, Vila J, Cole M, Sanberg CD, Sanberg PR, Bickford PC: Oxidative stress of neural, hematopoietic, and stem cells: protection ... These data suggest Stem- Kine supplementation increases circula- tion of cells capable of giving rise to hematopoietic- lineage cells in vitro. Stem- Kine augments circulation of cells with EPC phenotype Agents...

Ngày tải lên: 18/06/2014, 16:20

... Flk-1+CD31 -CD34- mesenchymal stem cells: feasibility and safety from monkey to human. Stem Cells Dev,2006,15(3):349-357 41. Devine SM, Bartholomew AM, Mahmud N, et al. Mesenchymal stem cells are ... of stem cells, it is very important to observe the survival and cura- tive effects of transplanted stem cells. Traditional immunohistochemical method can easily identify the transplanted cells ... circulation in the necrotic femoral head and sur- rounding tissues [4]. Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into a variety of cell types. In the field of...

Ngày tải lên: 25/10/2012, 11:18