cd34 mouse hematopoietic stem cells

... 2003, 61 (Suppl 1), 2-7 16 Chambers I The molecular basis of pluripotency in mouse embryonic stem cells Cloning Stem Cells 2004, 6, 386-391. 17 Cobb MH, Goldsmith EJ How MAP kinases are regulated ... H2O2-induced ROS generation in mouse embryonic stem cells using DCF-DA (10-5M), a fluorescent probe of intracellular H2O2 Production of ROS was detected in the cells exposed to H2O2 10-3 M for ... on mouse embryonic stem cells exposed to H 2 O 2 -induced oxidative stress 253Fig 5 Effect of dihydrotestosterone (DHT) on hydrogen peroxide (H2O2)-induced decrease of DNA synthesis A-D: The cells

Ngày tải lên: 07/08/2014, 20:23

... oxygen depletion/reoxygenation con-ditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated ... containing the conserved catalytic active site in cultured mouse embryonic stem cells Due to the importance of anti-oxidative stress mechanisms in stem cells, we have performed studies on this isoform ... differentiation Only cells within the 20th passage were used Anoxia/reoxygenation treatment The anoxic treatment of mouse embryonic stem cells was performed by incubating the cells in an anaerobic

Ngày tải lên: 10/08/2014, 05:21

... wherein hematopoietic stem cells (HSCs) differ-entiate into mature hematopoietic cells [1] It is a pro-cess controlled by complex interactions between numerous genetic processes in blood cells ... expression of CD34 and represents a heterogeneous cell population encompassing stem cells, early pluripotent progenitor cells, multipotent progeni-tor cells, and uncommitted differentiating cells [8] ... [9-18] Leukemia Stem Cells/Cancer Stem Cells (LSC/CSC) The cancer stem cell hypothesis postulates that cancer can be initiated, sustained and maintained by a small number of malignant cells that have

Ngày tải lên: 12/08/2014, 23:23

... deficient cells, an idea that is consistent with enhanced H3K27me3 at pericentric regions in these cells [25] Conclusion We show that the timing of mouse satellite replication is altered in ES cells ... pluripotent ES cells.</p> Abstract Background: The time of locus replication during S-phase is tightly regulated and correlates with chromatin state Embryonic stem (ES) cells have an unusual ... replication in early S-phase in ES cells, while replication timing is delayed in cells with restricted developmental options Results: Here we used a panel of mutant mouse ES cell lines lacking important

Ngày tải lên: 14/08/2014, 08:20

... culture of pluripotent stem cells……… 1 1.2 Characteristics of mouse embryonic stem (ES) cells………8 1.3 Application of ES cells……… 10 1.4 Molecular characteristics of ES cells……… …12 Chapter II ... implantation mouse embryos, and these cells are called EpiSCs (post-implantation epiblast derived stem cells) (Brons et al., 2007) These cells display profound differences from mouse ES cells in ... cells, embryonic stem cells, from the mouse embryo Embryonic stem cell lines are derived from the inner cell mass (ICM) of the mouse blastocyst at embryonic day 3.5 (E3.5) These cells were initially

Ngày tải lên: 10/09/2015, 15:51

... pluripotency in mouse embryonic stem cells Nature Genetics 38(4):431-40 *these authors contributed equally to this work Trang 20CHAPTER 1 GENERAL INTRODUCTION 1.1 Stem cells Stem cells are classically ... Stat3 and Smad1 in mouse embryonic stem cells as annotated in NCBI Trang 14LIST OF FIGURES Figure 1.1 A schematic view of mouse pre-implantation development 1.2 Mouse embryonic stem cell (mESC) ... LIST OF PUBLICATIONS xix CHAPTER I General Introduction 1.1 Stem cells 1 1.2 Embryonic stem cells (ESCs) 2 1.3 Properties of mouse ESCs (mESCs) 3 1.3.1 Differentiation of mESCs 4 1.4 Maintaining

Ngày tải lên: 11/09/2015, 16:03

... chosen for this lineage, namely the pancreatic cells types comprising of the alpha, beta and acinar cell types and the mouse mescenchymal stem cells The third layer, mesoderm is the middle layer ... allowed to differentiate so that there will be a mixture of stem cells and differentiated cells CDy9 was added to this semi-differentiated cells and it was observed that CDy9 only stained the miPSC ... differentiated cells and GFP-miPSCs (a) and (b) are 10X and 40 X images respectively CDy9 signal is only noticed for the stem cells which are confirmed by the GFP signal The differentiated cells are

Ngày tải lên: 02/10/2015, 17:14

... Stem Cells Into Cardiomyocytes 2002 Circ Res 91:189-201 29) I S Schroeder, A Rolletschek, P Blyszczuk, G Kania, A M Wobus Differentiation of mouse embryonic stem cells to insulin-producing cells ... staining and interaction with the stem cells and a better understanding of what is resulting in pluripotent stem cells to bind specifically to CDy9 as compared to the other cells This may eventually ... T Nakano, Embryonic Stem cell Internal Medicine 2001 Vol 40, No 3 4) JM Slack Stem Cells in Epithelial Tissues 2000 Science 287, 1431–1433 5) A I Caplan Mesenchymal stem cells J Orthop Res.,

Ngày tải lên: 02/10/2015, 17:14

... adult stem cells are hematopoietic stem cells which are formed in the bone marrow These cells play a role by differentiating and replacing the blood cells in the body Other types of adult stem cells ... function (1 - 3) Typically, stem cells can be broadly classified into 2 main groups, the embryonic stem cells and the somatic or adult stem cells The latter, adult stem cells are found in various ... mouse pluripotent stem cells, CDy9 The potential of CDy9 to detect single stem cells was also demonstrated via single cell PCR experiment This may then allow the purification of single stem cells

Ngày tải lên: 02/10/2015, 17:14

... Illustration 1 – Invitrogen BlockIT tetracycline inducible short, hairpin RNA system Trang 9Introduction Mouse embryonic stem cells (mESC) are uniquely capable of differentiating into all somatic cell ... Stromal-Derived Inducing Activity (SDIA) co-culture Mouse embryonic stem cells seeded onto PA6; the latter were visible as flat, dark patches of cells beyond the bright, round cluster morphology ... Embryonic stem cells transfected with FAM-labelled negative control retained a normal mESC morphology 6 days post-transfection, while siOct or siSox transfected cells differentiated into cells with

Ngày tải lên: 27/11/2015, 11:25

... centers on hematopoietic stem cells (HSCs), which are essential blood stem cells located in adult bone marrow These cells play a crucial role in regenerating blood and immune system cells Investigations ... system disorders.The different classes of hematopoietic stem cells can be categorized by their potency and structured in a tree [42,45] (see Figure 1-1) At the root of the hierarchy are the Hematopoietic ... types.M Kroiss, Predicting the Lineage Choice of Hematopoietic Stem Cells, Using recurrent neural networks (RNNs) to predict the lineage choice of stem cells reveals key trends in gene expression

Ngày tải lên: 10/05/2022, 14:58

... Rubin E Effects of ethanol on mouse embryonic stem cells Alcohol Clin Exp Res 2009;33:2172–2179 Bain G, Kitchens D, Yao M, Huettner JE, Gottlieb DI Embryonic stem cells express neuronal properties ... behaviour of stem cells is strongly affected by their local environment or niche (Watt and Driskell 2010) Some aspects of the stem cell environment that are known to influence self-renewal and stem cell ... and neurons from mouse embryonic stem cells Nat Protoc 2007;2:1034–1043. Bratt-Leal AM, Carpenedo RL, McDevitt TC Engineering the embryoid body microenvironment to direct embryoid stem cell differentiation

Ngày tải lên: 02/11/2022, 10:47

... Level of Hematopoietic Stem Cells: Inference from Differentiation in Daughter Cell and Granddaughter Cell Pairs J Exp Med 199, 295–302 (2004). 6 Ema, H et al Adult mouse hematopoietic stem cells: ... of hematopoietic cells (Gr1+ Mac1+ monocytes, B220+ B cells, CD3+ T cells) (Fig. 2E, Fig. S3B) These results implied stable integration of the GFP reporter gene into neonatal hematopoietic stem/progenitor ... analysis of GFP+ cells in various hematopoietic organs at 24 weeks post-injection CD150+ CD34−/lowKSL represent primitive hematopoietic stem cell fraction (considered a more highly purified stem cell

Ngày tải lên: 19/11/2022, 11:40

... pluripotent and differentiated cells Pluripotent cells were prepared by culturing EB3 cells in LIF(+ ) medium for 3 d Differentiated cells were prepared by culturing pluripotent cells in LIF(− ) medium ... EB3 cells, their cell membrane fraction, their cytosol fraction, Cx30.3 overexpressing EB3 cells, the kidney and the spleen of a C57BL/6 N mouse Mouse kidney was positive control, while mouse ... of pluripotent EB3 cells were treated with trypsin to dissociate them into single-cells and applied to a cell sorter SSEA1 (stage-specific mouse embryonic antigen) stained cells were also prepared

Ngày tải lên: 19/11/2022, 11:41

... Trang 1R E S E A R C H Open AccessDistinct 5-methylcytosine profiles in poly(A) RNA from mouse embryonic stem cells and brain Thomas Amort1†, Dietmar Rieder2†, Alexandra Wille1, Daria Khokhlova-Cubberley3, ... have carried out an unbiased global analysis of m5C in total and nuclear poly(A) RNA of mouse embryonic stem cells and murine brain We show that there are intriguing differences in these samples ... in mammals Keywords: RNA methylation, 5-Methylcytosine, m5C, Epitranscriptome, Embryonic stem cells, Mouse brain, m6A, RNA binding proteins, Bisulfite sequencing, meRIP Background Posttranscriptional

Ngày tải lên: 24/11/2022, 17:43

... and MPP cells in BM among donor-derived Ly 5.1 + LSK cells in young and old recipient (Ly 5.2 + ) mice. D Frequency of young B cells, T cells and myeloid cells among donor-derived Ly 5.1 + cells ... for early hematopoietic progenitor cells (LSK) cells in OPN / animals (Nilsson et al, 2005) Y?O animals presented with an increase in the frequency of myeloid cells over lymphoid T cells, while ... 2016 | Accepted 18 January 2017 Introduction One cause of aging of the hematopoietic system is aging of hematopoietic stem cells (HSCs) While upon aging the number of phenotypic HSCs increases,

Ngày tải lên: 04/12/2022, 15:58

... restricted to its subsets (e.g., fractions of Ascells), or extended over the entirety of GFRa1+cells (Yoshida, 2017) Interestingly, this stem cell system appears not to rely on asymmetric division ... restricted expression to a subset of GFRa1+cells In vitro and in vivo analyses illustrate the key roles of Shisa6 and SHISA6+cells in this stem cell system RESULTS Wnt/b-Catenin Signaling Promotes ... expres-sion suggests that NGN3+cells may receive a stronger signal than GFRa1+cells Next, we tested the effect of Wnt/b-catenin signaling in vitro using germline stem (GS) cells: GDNF-dependent cultures

Ngày tải lên: 04/12/2022, 16:14

... investigated its role in the response of hematopoietic stem cells (HSC) to liver fibrosis in mice Methods: Purified (c-kit+/sca1+/lin-) hematopoietic stem cells (HSC) were repeatedly infused into ... therapeutic paradigm for the use of hematopoietic stem cells in this setting It has previously been proposed that injected bone marrow cells differentiate into anti-fibrotic cells of a monocyte/macrophage ... Hepatocyte Growth Factor which recruit human CD34+ progenitors32 We have previously reported that the adhesion of human hematopoietic (CD34+) stem cells to human liver compartments is integrin

Ngày tải lên: 26/07/2023, 07:40

... fulfilling of their functions [3,4] Like many other cancer stem cells, a hypothesis of prostate cancer stem cells (PCSCs) originated from nor-mal stem cells has been proposed based on their highly * Correspondence: ... P63+ basal/stem cells and to examine whether these cancer cells can maintain stem cell characteristics after metas-tasis More importantly, we intended to elucidate whether P63+ basal/stem cells can ... overexpression P63+basal/stem cells were transformed into AR+or AR -cancer cells but rarely into ChgA+neuroendocrine cancer cells Conventional human prostate cancer cells include malignant cells of luminal,

Ngày tải lên: 10/08/2014, 05:21

... statistical significance. Results Stem- Kine mobilizes CD34 and CD133 Cells Quantification of peripheral blood cells expressing the hematopoietic stem cell markers CD133 and CD34 was performed at day ... human stem cells. Stem Cells Dev 2006, 15(1):118-23. 77. Shytle RD, Ehrhart J, Tan J, Vila J, Cole M, Sanberg CD, Sanberg PR, Bickford PC: Oxidative stress of neural, hematopoietic, and stem cells: protection ... These data suggest Stem- Kine supplementation increases circula- tion of cells capable of giving rise to hematopoietic- lineage cells in vitro. Stem- Kine augments circulation of cells with EPC phenotype Agents...

Ngày tải lên: 18/06/2014, 16:20