cloning pcr and in vitro mutagenesis

... cross-linking both in the amorphous and crystalline regions when it penetrates the granules through channels and cavities resulting in inhibiting the granule swelling [21] As a result, the combination ... starches including cassava and potato starches The different results obtained were due to the difference in moisture content and heating temperature used in those studies [20] The results in Fig indicate ... suspended in 25 mL of water, corrected for 14% moisture, by jogging 10 times with a paddle The procedure used was: 50 oC for min, heating to 95 oC at 12 oC/min, maintained for 2.5 min, and cooling...

Ngày tải lên: 16/12/2017, 18:06

... one involving CaM and CaM-related proteins, and the other involving CDPKs Both of these pathways cross-talk at various points in the signaling cascade [9] The presence of CaM has been detected in ... protein kinases are involved in stress signaling pathways [29] and recent work points towards the involvement of Ca2+/CaM-dependent protein kinases in stress signaling [16], changes in the level of ... containing lg purified p40 (fraction A) and those containing lg of both p40 and p72 PsCCaMK kinase (fraction B) were used for in vitro phosphorylation in the absence or presence of Ca2+, CaM, kinase...

Ngày tải lên: 18/03/2014, 01:20

... dinuclear Cu (OAc) (H O) (right). Trang 3The difference between the amount released at 48 h andthat contained in the original loading solution deter-mined Cu loading, resulting in values ranging ... synthesis, and metal loading RP conceived of the study, and participated in its design and coordination, carried out in vitro toxicity studies, SEM and EDX analysis and drafted the manuscript. Competing ... synthesis, characterization, and metal binding properties of our Cu-binding particles, as well as preliminary in vitro toxicity in cancer cells Results Synthesis and characterization of Cu-loaded...

Ngày tải lên: 21/06/2014, 02:20

... cell line in vivo and in vitro Based on these results, we hypothesized that the two different tumor cell lines, including GBC-SD and SGC-996, can exhibit significant different invasive ability and ... were incu-bated with 0.5% PAS for 10 min in a dark chamber and washing with distilled water for 3 min, finally all of these sections were counterstained with hematoxylin The Microvessel in marginal ... staining: 12 paraffin-embedded tissue speci-mens of tumor xenografts were deparaffinized, hydrated, and stained with H&E Companion serial section were stained with double staining of CD31 and...

Ngày tải lên: 10/08/2014, 10:21

... cytokine repertoire of DC matured in the presence of inflammatory stimuli com-prises pro-inflammatory cytokines and chemokines, including the T cell inhibitory cytokine IL-10, the Th-1 promoting ... PerCP-Cy5.5 and CD4 APC and acquired on a flow cytometer CD3+CD4+ lymphocytes were gated including the blasts and excluding CD209+ MDDC The percentage of cells showing decreased CFSE staining intensity ... IL-12+ and COX-2+cells, compared to the corresponding unstimulated cells (p < 0.05, data not shown) The dot plots in Fig 3A and 3B show the intracellular staining profiles of IL-12 and COX-2 in...

Ngày tải lên: 11/08/2014, 10:23

... treatment were used in the following in vivo and in vitro studies. 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen ... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation. IL10 producing CD4+ T cells showed a distinct ... minutes in dark. Cells were then re-suspended and permeabilized in Permeabilization Buffer (eBioscience) to facilitate the intracellular staining. PBMCs were stained with intracellular cytokines...

Ngày tải lên: 23/09/2015, 14:47

... nausea and vomiting that can be quite severe and require interrupting treatment A less frequent side effect is bone pain[47] Other rare side effects include vaginal bleeding, thrombophlebitis, and ... is excreted in the bile and eliminated through the faces, with small amounts eliminated in the urine[44, 78, 83] Following oral administration of tamoxifen, elimination is biphasic and is dependent ... from the gastrointestinal tract[69] Its bioavailability is high (approximately 100%) and independent of dose, suggesting minimal first-pass metabolism[70, 71] Following single-dose administration...

Ngày tải lên: 09/10/2015, 11:24

... consequences arising from the use of information contained in these “Just Accepted” manuscripts. Trang 2Aβ41 Aggregates more Like Aβ40 than Aβ42: In silico and in vitro Study Nguyen Hoang Linh1,2, Tran ... of Ala42 in structures and aggregation kinetics of Aβ It would be interesting to know to what extent the interchange of Ile41 and Ala42 in sequence alters aggregation properties AUTHOR INFORMATION ... eigenvalues V1 and V2 in the dihedral principal component analysis (dPCA)34 were used as reaction coordinates for constructing the free-energy surface In vitro Experiment Synthesis and Purification...

Ngày tải lên: 12/12/2017, 10:09

... (amantadine and rimantadine) and neuraminidase inhibitors (NAIs; zanamivir and oseltamivir) are currently used for the chemoprophylaxis and treatment of influenza [9] The drugs should be administered ... administered within 48 hours of infection to get the optimum results Amantadine binds to and blocks the M2 ion channel proteins function and thereby inhibits viral replication within infected cells ... against the virus to control and treat infections Traditionally, the generation of new drugs involves screening hundreds of thousands of components against desired targets via in vitro screening...

Ngày tải lên: 12/08/2014, 04:21

... radioactivity indicated here, you should maintain identical ratios in first- and second-strand syntheses Our protocol results in ds cDNA of an SA of 6.67 x lo5 dpm/l.tg Single-stranded cDNA (first-strand ... 7.5, and 20% polyethylene glycol (PEG8000) 29 Photoprobe Biotin and Avidin D agarose resin (Vector Laboratories, Burlingame, CA): Resin is prepared by washing the slurry three to four times in ... 1OX kinase buffer, pL of 100 mA4rATP, pL of H20, and p.L of 10 U&L polynucleotide kinase 15 Incubate kinase reaction for 30 at 37OC 16 Heat inactivate the polynucleotide kinase by incubating the...

Ngày tải lên: 11/04/2014, 09:47

... gieo với mật độ dầy nên cấy chuyền Hạt lan Mini Dendrobium nẩy mầm sau 30 ngày phương pháp nuôi cấy mô Lan Mini Dendrobium phát triển mạnh điều kiện invitro Môi trường cấy chuyền môi trường gieo ... từ 1-2%) hạt lan chứa anbumin phôi chưa phân hóa, có kích thước nhỏ nên không chứa chất dự trữ Vì tự nhiên để hạt lan nẩy mầm cần cộng sinh với nấm Có loại nấm cộng sinh nhiều giống lan khác biết: ... paraffin - Đem để vào chỗ tối mát tuần Sau đem ánh sáng (cường độ khoảng 2000 lux = bóng đèn neon để cách khoảng 30 cm) Nếu làm tốt không nhiễm, đồng thời hạt thụ tốt vòng 30 - 40 ngày (đối với Mini...

Ngày tải lên: 04/07/2014, 23:20

... contains a highly active lysoPC acyl transferase [25,26] Acyl group incorporation into PE also occurred in all fractions, with similar rates in the ER- and plasma membranecontaining fractions In ... two-phase partition alone or in combination Physiol Plant 1992, 84:121-133 Sommarin M, Sandelius AS: Phosphatidylinositol and phosphatidylinositolphosphate kinases in plant plasma-membranes Biochim ... to the varying conditions in the plant environment The adjustments include selective lipid degradation, increased incorporation of certain lipid classes and/ or lipid molecular species and possibly...

Ngày tải lên: 12/08/2014, 05:20

... software, managing user accounts, and backing up and restoring files Many more topics are covered in this chapter Chapter details the steps involved in planning and building a network and planning for ... Installing and Configuring Servers Installing and Configuring Application Software Creating and Maintaining User Accounts Backing Up and Restoring Files ... 302 Installing WU-FTPD 303 Installing the binary RPM 304 Installing and building the source RPM 304 Installing and building...

Ngày tải lên: 12/12/2013, 22:15

... phosphate-buffered saline (PBS), and resuspended at × 106 cells /mL in Annexin-V binding buffer The supernatant (100 μL/tube) was incubated with μL of Annexin-V-FITC (Invitrogen, USA) and μL of PI (Invitrogen, ... (GIBCO, USA) containing 10% heat-inactivated fetal bovine serum (Nuoding, China), and the HT-29 cells were grown in DMEM medium (GIBCO, USA) also containing 10% heat-inactivated fetal bovine serum All ... then evaluated by in vitro testing in human cancer cell lines We observed that 198 RGD-M-LCL had increased anti-proliferative activity and increased the induction of apoptosis in cancer cells compared...

Ngày tải lên: 26/10/2012, 08:57

... functional domain mapping of proteins where fragments that represent protein domains are expressed and functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction ... strand of DNA suitable for subsequent DNA sequencing Analysis, sequencing and in vitro expression of PCR products 99 Template DNA PCR with one biotinylated primer B Biotinylated PCR product Bind ... (A) Initial PCR amplification product using two original flanking primers (primers and 2); (B) nested PCR from the primary PCR amplification product using one original flanking primer (primer 1) and...

Ngày tải lên: 25/10/2013, 22:20

... configuring servers N Installing and configuring application software N Creating and maintaining user accounts N Backing up and restoring files N Monitoring and tuning performance N Configuring a secure ... you prevent intrusion into non-Linux machines that may reside on your network Linux is designed from the beginning with security in mind, and in all of your tasks you should maintain that same ... planning and preparing for your new network installation Deciding What Kind of Network You Need Linux is, by definition and right out of the box, a network operating system It is also nearly infinitely...

Ngày tải lên: 12/12/2013, 22:15

... EF-2 in the mitochondrial apoptotic response induced by the toxin remains to be determined Finally, we assign an important role to the endosomal acidic cathepsins B and D in increasing the in vitro ... death (5 postinjection), suggesting a high binding capacity of ETA in hepatic parenchyma Indeed, injection of the toxin into mice has been shown to result in an early and profound inhibition of ... endosomes containing in vivo internalized ETA The endosomal fraction was isolated 30 after the administration of ETA, and then resuspended in 0.15 M KCl containing mM MgCl2 and, when indicated, 50...

Ngày tải lên: 18/02/2014, 04:20

... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and ... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids...

Ngày tải lên: 18/02/2014, 08:20

... staining patterns (comparing and 2) and by the yellow color resulting from combination of green and red fluorescence in that iPLA2c is localized, in large part, to the mitochondrial compartment in ... iPLA2a (patatin, found in potato tubers) [28] and iPLA2b [23] – iPLA2c contains a consensus site for nucleotide binding and a lipase consensus motif in its C-terminal half [26] Although the intracellular ... 88, 77, 74 and 63 kDa polypeptides [26] However, in initial studies in baculoviral and in vitro rabbit reticulocyte lysate systems, we unexpectedly observed that constructs containing the full-length...

Ngày tải lên: 19/02/2014, 16:20

... template and binding properties of mutated and deleted RNA fragments of the 3¢-end of the minus-strand HCV RNA in further detail This study should lead to interesting interpretations since it ... domain in the 3¢-end of the HCV minus-strand RNA inhibits in vitro initiation of RNA synthesis by the viral polymerase [19] Kashiwagi et al [20] studied RNA synthesis by recombinant HCV NS5B using ... unavailable In the present study we investigated the involvement of sequences and ⁄ or structures in RNA synthesis directed in vitro by HCV NS5B by using new mutants of the 3¢-end of minus-strand RNA...

Ngày tải lên: 20/02/2014, 01:20