biochemical histological and molecular analyses

... the biochemical [Table 2] and cellular [Table 3] inflammatory profiles previously described Weight gain and virus recovery in PVM-infected neonatal and weanling mice Normal uninfected neonatal and ... by dissection and placed into ml RPMI 1640 with 5% fetal bovine serum (FBS) The lungs were teased and cut into pieces and then digested with mL RPMI with 5% FBS, 20 μg/mL DNAse I and mg/mL collagenase ... formalin The lungs and heart were removed and fixed overnight in 10% phosphate-buffered formalin at 4°C Samples were paraffin-embedded, sectioned, and stained with hematoxylin and eosin (Histoserv,...

Ngày tải lên: 12/08/2014, 02:20

... skeletal and smooth muscle [20] Two different isoforms of MLC-2 are typically expressed in the atria and ventricles of the mammalian heart during embryonic development and maturation, and later ... β-galactosidase enzyme detection assays and drafted the manuscript YS designed and cloned the plasmids and harvested tissues BDT performed the RNA transcript analysis and helped to draft the manuscript ... Fellowship Award-Florida and Puerto Rico Affiliate (to CAP), the NIH National Heart, Lung, and Blood Institute grant PO1 HL59412; National Institute of Diabetes and Digestive and Kidney Diseases grant...

Ngày tải lên: 14/08/2014, 19:22

... immunohistochemically, biochemical analysis was performed on J20 APP-tg mice using specific ELISAs for Aβx-40 and Aβx42 There was a non-significant reduction of Aβ40 and Aβ42 TBS and guanidine soluble ... immunohistochemistry and ELISAs, and guided or performed image analysis, analyzed data and drafted the manuscript LJ performed tissue preparation, immunohistochemistry, image analysisand ELISAs KT carried ... aseptically removed and placed in RPMI on ice for cell culture studies The brain was removed and divided sagittally into two hemispheres One hemi-brain, as well as pieces of liver, kidney and spleen was...

Ngày tải lên: 19/06/2014, 22:20

... have been identified: type I and type II receptors (IL-RI and IL-RII) [26] IL-1β binds IL-1RI and upon IL-1 binding, IL-1RI recruits the accessory protein IL-1R-AcP, and initiates a stimulatory ... to five to a cage and maintained on ad libitum and water with a 12 h light/dark cycle Passive immunizations Groups of APP/IL-1 R1-/- mice and APP/IL-1 R1+/- littermates (males and females, 6-month-old ... at various ages (6 months, months and 15 months of age) were killed and the levels of both SDSsoluble (SDS) and SDS-insoluble FA-extractable fractions of Aβ40 and Aβ42 were analyzed by ELISA As...

Ngày tải lên: 19/06/2014, 22:20

... following exposure to ozone, NNK, and DBP, and combined treatments of NNK and DBP on ozone for 32- and 52-wk The procedures for isolating lymphocytes from spleen and culturing hprt mutant T-cell ... that additive and/ or synergistic responses occurred when both mice sexes were exposed to ozone, NNK, and DBP, and the combination of ozone, NNK, and DBP through chromosome aberration and supravital ... NNK, DBP, and combined treatment of NNK and DBP on ozone were assayed for the mutant frequency of 6thioguanine-resistant (TGr) spleen lymphocytes in male and female mice after 32- and 52-wk exposures...

Ngày tải lên: 07/08/2014, 18:20

... performed the real-time PCR and cell-based assays, and participated in respective data analyses GK established the Tg197 arthritis model and provided logistical support and intellectual contributions ... performed preclinical analyses and provided clinical specimens PG contributed to conception and design of the project, and organized for collaborative research with ED and KG, discussed the data ... increased by nearly 10-fold and 8-fold by IL-stimulated RA and OA SF cells, respectively Ele- Table Percentage of fibroblasts and contaminating cells in primary cultures of RA and OA synovial fibroblast...

Ngày tải lên: 09/08/2014, 14:22

... experiments and wrote the paper AJ performed the experiments analyzed data and wrote the paper CY performed experiments PS and NM designed the study and wrote the paper All authors read and approved ... ligands J Virol 2007, 81:8180-8191 53 Mandl JN, Barry AP, Vanderford TH, Kozyr N, Chavan R, Klucking S, Barrat FJ, Coffman RL, Staprans SI, Feinberg MB: Divergent TLR7 and TLR9 signaling and ... HLADR and PD-1, two well established markers associated with HIV disease progression [37,38], on both CD4 and CD8 cells Interestingly we found that upregulation of HLA-DR (Figure 5A and 5B) and...

Ngày tải lên: 11/08/2014, 21:21

... cytometric analyses, immunohistological sections and ELISAs, performed the statistics and drafted the manuscript BV conceived the study, participated in its design and coordination and helped ... activation and temperature Statistics and graphics were performed using the software packages SigmaStat and SigmaPlot (Jandel Corporation, San Rafael, CA, USA) times of 255 ± 35 s and 238 ± 58 ... paraffin-embedded tissue blocks, μm-sections were cut and stained with hematoxylin and eosin for histological analysis For immunohistochemical demonstration of P-selectin and PAI-1 expression, sections collected...

Ngày tải lên: 13/08/2014, 03:20

... Phatisawad, and Rapee Trichavaroj; Sean Hill, David Troelstrup, Erik Anderson, Andrew Williams, and Wichitra Apateerapong; and as well as acknowledge the SEARCH participants for their support and commitment ... subtype B) and V3 CM242 (subtype E) peptides, which distinguishes HIV-1 subtype B and E infection in Thai individuals and confirmed by sequencing, when indicated [19, 20] Specimens and HIV DNA ... clades A, E, and B; and demonstrated equivalent amplification of the target gene Separation of PBMC Subsets and HIV DNA Analaysis Because HIV DNA is present in both lymphocytes and monocytes,...

Ngày tải lên: 31/10/2012, 15:34

... vực có liên quan đến an ninh trật tự biểu mẫu thống kê phục vụ mặt công tác nghiệp vụ lực lượng CAND,… Công ty có nhiệm vụ in ấn tài liệu tuyên truyền, thông báo tình hình an ninh trật tự hàng...

Ngày tải lên: 25/01/2013, 09:29

... WHILE-LISTENING Look at the pictures and describe them WHILE-LISTENING Pre order these pictures WHILE-LISTENING Listen and order these pictures WHILE-LISTENING Listen again and decide whether the statements ... to work T F POST-LISTENING V HOMEWORK - T asks ss to remember the story about Mr Lam and write about him and his daily routine ... stall - He drives passengers to everywhere they want HE IS A CYCLO DRIVER PRE-LISTENING Listen and repeat district:/'distrikt/ park:/pɑ:k/ routine:/ru:'ti:n/ pedal:/'pedl/ office:/'ɔfis/ food...

Ngày tải lên: 21/06/2013, 01:26

... the passage and find all the verbs that are used in past simple and the connectors in the story Simple past Regular verbs land stare announce Irregular verbs take begin S + Ved landed stared ... up a narrative about a hotel fire/ and / everybody / be / It/be/Saturday evening the discotheque / ground floor It / be / crowded /with people They / dance / and/ sing happily./ Suddenly / we ... up a narrative about a hotel fire/ and / everybody / be / It/be/Saturday evening the discotheque / ground floor It / be / crowded /with people They / dance / and/ sing happily./ Suddenly / we...

Ngày tải lên: 18/09/2013, 05:10

Ngày tải lên: 19/09/2013, 14:10

... traffic increases and the load on the database and Web servers goes up The JSP architecture, on the other hand, involves more than one level, immediately making it more scalable and maintainable ... format float and long − used to store floating−point numbers boolean − used to store "true" and "false" values (note that unlike languages like C and PHP, JSP does not recognize => true and => false) ... " and " + beta + " is " + Sum); %> And the output is: The sum of 45 and 34 is 79 In this case, we've simply defined two variables as integer values, assigned values to them, and...

Ngày tải lên: 19/10/2013, 02:15

Ngày tải lên: 28/10/2013, 17:15

... activities and time’s activities of Linh -2 ms for teams go to the board and write answers -team which more correct answer is winner * checking instruction (p.) *after ms T check answers and find ... BRAIN STORM -ask ss think about some their activities in a day in m -after m t call on some ss and ask them about their activities: T: Lan, what time you often get up? Lan:: I often get up at...

Ngày tải lên: 02/12/2013, 19:11