DNA vaccine

DNA vaccine and Application of DNA vaccine in “Fisheries”...  DNA vaccine is DNA sequence used as a vaccine  This DNA sequence code for antigenic protein of pathogen  As this DNA i

DNA vaccine and Application of

DNA vaccine in “Fisheries”

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What is DNA vaccine?

 DNA vaccine is DNA sequence used

as a vaccine

 This DNA sequence code for

antigenic protein of pathogen

 As this DNA inserted into cells it is

translated to form antigenic protein

As this protein is foreign to cells, so

immune response raised against this

protein.

 In this way, DNA vaccine provide

immunity against that pathogen.

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by Activated User

Uses only the DNA from infectious organisms

Uses only the DNA from infectious organisms

Avoid the risk of using actual infectious organisms

Avoid the risk of using actual infectious organisms Provide both

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How DNA vaccine works

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Some antigen require processing which sometime does not occur

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2007 NIAID DNA Vaccine for H5N1 Avian

Influenza Enters Human Trial

NIAID DNA Vaccine for H5N1 Avian Influenza Enters Human Trial

8/2007

DNA vaccination against multiple Sclerosis was reported as being effective

DNA vaccination against multiple Sclerosis was reported as being effective

2007 Trail DNA-MVA v

Current clinical trails

Cancer, Malaria can be available

DNA vaccination against infectious diseases such as AIDS, Rabies,

Cancer, Malaria can be available

Application of DNA vaccine in “Fisheries”

IHN is an RNA stranded virus The genome codes for 6 different proteins are given

Five are structural proteins and one is a non structural protein

Must know which gene codes for the protein that is involved in the immunetic response It is the glyco protein spike on the outside of the virus, denoted by the G

protein, that is the immunetic protein of the virus

Insect G-gene into DNA plasmid form Recombinant DNA

Inject DNA vaccine into Fish

G-gene construct shows good surface expression, which is believed to be a key feature to the effectiveness of the vaccine You also see a very heavy monocytic

cell infiltrate, most likely white blood cells

Quer ies

?

http://wikipedia.org

Tom Christian Tonheim*, Jarl Bøgwald, Roy Ambli Dalmo

** What happens to the DNA vaccine in fish? 19 March 2008

http

://www.bcic.ca/research-institutions/research-development/ aqua-e-fund/aquaculture-workshops/ihnv-summit

http:// www.immune.org.nz/print.asp?t=919

http://dna-vaccine.net/

References