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Lecture Human anatomy and physiology - Chapter 3: Cells (part a)

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Chapter 3 - Cells: The living units (part a). Just as bricks and timbers are the structural units of a house, cells are the structural units of all living things, from one-celled “generalists” like amoebas to complex multicellular organisms such as humans, dogs, and trees. The human body has 50 to 100 trillion of these tiny building blocks. This chapter focuses on structures and functions shared by all cells.

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 Cells: The Living Units

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 The cell is the smallest structural and 

functional living unit 

 Organismal functions depend on individual and collective cell functions

 Biochemical activities of cells are dictated by their specific sub cellular structures called organelles

 

 

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 Over 200 different types of human cells

 Types differ in size, shape, subcellular components, and functions

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Copyright © 2010 Pearson Education, Inc.

Fibroblasts

Erythrocytes

Epithelial cells

(d) Cell that fights disease

Macrophage

Figure 3.1

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  All cells have some common structures and functions

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Copyright © 2010 Pearson Education, Inc. Figure 3.2

Secretion being released from cell

by exocytosis Peroxisome

Ribosomes

Rough endoplasmic reticulum

Nucleus

Nuclear envelope Chromatin

Golgi apparatus

Nucleolus Smooth endoplasmic

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 Bimolecular layer of lipids and proteins in a constantly changing fluid mosaic 

 Plays a dynamic role in cellular activity

 Separates intracellular fluid (ICF) from 

extracellular fluid (ECF)

◦ Interstitial fluid (IF) = ECF that surrounds cells

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Copyright © 2010 Pearson Education, Inc. Figure 3.3

Integral proteins

Extracellular fluid (watery environment)

Cytoplasm (watery environment)

Polar head of phospholipid molecule Glycolipid

Cholesterol

Peripheral proteins

Bimolecular lipid layer containing proteins

Inward-facing layer of

phospholipids

facing layer of phospholipids

Outward-Carbohydrate

of glycocalyx

Glycoprotein

Filament of cytoskeleton

Nonpolar tail of phospholipid molecule

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1. Transport

2. Receptors for signal transduction

3. Attachment to cytoskeleton and extracellular 

matrix

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Copyright © 2010 Pearson Education, Inc. Figure 3.4a

A protein (left) that spans the membrane may provide a hydrophilic channel across the membrane that is selective for a

particular solute Some transport proteins (right) hydrolyze ATP as an energy source

to actively pump substances across the membrane.

(a) Transport

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Copyright © 2010 Pearson Education, Inc. Figure 3.4b

A membrane protein exposed to the outside of the cell may have a binding site with a specific shape that fits the shape of a chemical messenger, such

as a hormone The external signal may cause a change in shape in the protein that initiates a chain of chemical

reactions in the cell.

(b) Receptors for signal transduction

Signal

Receptor

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Copyright © 2010 Pearson Education, Inc. Figure 3.4c

Elements of the cytoskeleton (cell’s internal supports) and the extracellular matrix (fibers and other substances outside the cell) may be anchored to membrane proteins, which help maintain cell shape and fix the location of certain membrane proteins Others play a role in cell movement or bind adjacent cells

together.

(c) Attachment to the cytoskeleton and extracellular matrix (ECM)

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4. Enzymatic activity

5. Intercellular joining

6. Cell­cell recognition

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Copyright © 2010 Pearson Education, Inc. Figure 3.4d

A protein built into the membrane may

be an enzyme with its active site exposed to substances in the adjacent solution In some cases, several

enzymes in a membrane act as a team that catalyzes sequential steps of a metabolic pathway as indicated (left to right) here.

(d) Enzymatic activity

Enzymes

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Copyright © 2010 Pearson Education, Inc. Figure 3.4e

Membrane proteins of adjacent cells may be hooked together in various kinds of intercellular junctions Some membrane proteins (CAMs) of this group provide temporary binding sites that guide cell migration and other

cell-to-cell interactions.

CAMs

(e) Intercellular joining

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Copyright © 2010 Pearson Education, Inc. Figure 3.4f

Some glycoproteins (proteins bonded

to short chains of sugars) serve as identification tags that are specifically recognized by other cells.

(f) Cell-cell recognition

Glycoprotein

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 Plasma membranes are selectively permeable

 Some molecules easily pass through the 

membrane; others do not

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Simple diffusion

Carrier­mediated facilitated diffusion

Channel­mediated facilitated diffusion

Osmosis

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 Nonpolar lipid­soluble (hydrophobic) substances diffuse directly through the phospholipid bilayer

Diffusion is the movement of solutes from a 

solution of higher concentration to that of a  lower concentration

PLAY Animation: Diffusion

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Copyright © 2010 Pearson Education, Inc. Figure 3.7a

Extracellular fluid

soluble solutes

Lipid-Cytoplasm

(a) Simple diffusion of fat-soluble molecules

directly through the phospholipid bilayer

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 Certain lipophobic molecules (e.g., glucose, amino acids, and ions) use carrier proteins or channel 

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 Transmembrane integral proteins transport 

specific polar molecules (e.g., sugars and amino acids)

 Binding of substrate causes shape change in 

carrier 

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Copyright © 2010 Pearson Education, Inc. Figure 3.7b

Lipid-insoluble solutes (such as sugars or amino acids)

(b) Carrier-mediated facilitated diffusion via a protein

carrier specific for one chemical; binding of substrate causes shape change in transport protein

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 Aqueous channels formed by transmembrane proteins selectively transport ions or water

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Copyright © 2010 Pearson Education, Inc. Figure 3.7c

Small insoluble solutes

lipid-(c) Channel-mediated facilitated diffusion

through a channel protein; mostly ions selected on basis of size and charge

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 Movement of solvent (water) from a solution of  low concentration to that of a higher 

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Copyright © 2010 Pearson Education, Inc. Figure 3.7d

Water molecules

Lipid billayer

Aquaporin

(d) Osmosis, diffusion of a solvent such as

water through a specific channel protein (aquaporin) or through the lipid bilayer

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 Water concentration is determined by solute 

concentration because solute particles displace water molecules

 Osmolarity: The measure of total concentration of solute particles

 

 When solutions of different osmolarity are 

separated by a membrane, osmosis occurs until equilibrium is reached

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Copyright © 2010 Pearson Education, Inc. Figure 3.8a

(a) Membrane permeable to both solutes and water

Solute and water molecules move down their concentration gradients

in opposite directions Fluid volume remains the same in both compartments.

Left compartment:

Solution with lower osmolarity

Right compartment:

Solution with greater osmolarity

Membrane

H 2 O Solute

Solute molecules (sugar)

Both solutions have the same osmolarity: volume unchanged

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Copyright © 2010 Pearson Education, Inc. Figure 3.8b

(b) Membrane permeable to water, impermeable to solutes

Both solutions have identical osmolarity, but volume of the solution on the right is greater because only water is

free to move

Solute molecules are prevented from moving but water moves by osmosis.

Volume increases in the compartment with the higher osmolarity.

Left compartment

Right compartment

Membrane

Solute molecules (sugar)

H 2 O

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 When osmosis occurs, water enters or leaves a cell

 Change in cell volume disrupts cell function

PLAY Animation: Osmosis

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 Tonicity: The ability of a solution to cause a cell to shrink or swell 

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Copyright © 2010 Pearson Education, Inc. Figure 3.9

Cells retain their normal size and

shape in isotonic solutions (same

solute/water concentration as inside

cells; water moves in and out).

Cells lose water by osmosis and shrink in a hypertonic solution (contains a higher concentration

of solutes than are present inside

the cells).

Cells take on water by osmosis until they become bloated and burst (lyse)

in a hypotonic solution (contains a lower concentration of solutes than are present in cells).

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