Postmortem histological evaluation of the repaired chondral defects 6 weeks after stem cell injection.. H&E staining A, IHC for collagen type I assessment B, IHC for collagen type II ass
Trang 1Figure 3-17 Postmortem histological evaluation of the repaired chondral defects 6 weeks after stem cell injection
H&E staining (A), IHC for collagen type I assessment (B), IHC for collagen type II assessment (C), Masson’s trichrome for cartilage detection (D),
Toluidine blue staining for collagen type II detection (E), Safranin O staining for cartilage matrix production evaluation (F) Arrowheads indicate the border
of repaired cartilage
Serial sectioning and Prussian blue staining were performed to confirm the presence of the labeled cells in the repaired cartilage Results as shown in Figure 3.18, 3.19 and 3.20 also confirmed the presence of the labeled MSCs
in the biopsies from the surgical scars and para-patellar fat tissues
respectively
Figure 3-18 Prussian blue staining of the repaired cartilage defect