bone marrow peripheral blood stem cell

... prerequisite for ASCT, hematopoietic stem cellsmust be available Peripheral blood stem cells (PBSCs) have become the most widely used source for hematopoietic stem cells in the setting of HD/ASCT ... the impact of CD34+ cell dose is marginal, G-CSF significantly accelerates the leukocyte recovery Keywords: Peripheral blood stem cells, Insufficient graft, Autologous stem-cell transplantation, ... followed by autologous blood stem-cell transplantation (ASCT) is the standard treatment for multiple myeloma (MM) patients However, the collection of sufficient peripheral blood stem cell (PBSC) grafts

Ngày tải lên: 30/05/2020, 21:57

... human granulocyte-colony stimulating factor (rhG-CSF), hematopoietic stem cell donor, follow-up, peripheral blood stem cells, bone marrow Word count: Abstract: 217 words Main text: 3,522 words Number ... hematopoietic stem cell donation Follow-up is indicated to detect potential long-term risks for donors We sent a follow-up questionnaire to 15,445 donors of peripheral blood stem cells (PBSC) or bone marrow ... Trang 4Highlights  Retrospective survey of 15,445 individuals who donated peripheral blood stem cells (PBSC) or bone marrow (BM) between 1992 and 2009  Almost 95% of responders assessed their

Ngày tải lên: 04/12/2022, 16:09

... epidermal cells in wound healing Key words: Peripheral blood stem cells; Mesenchymal stem cells; Hemopoietic stem cells; Stem cell plasticity; Common progenitor; Wound healing Introduction Adult bone ... bone marrow shelters different types of stem cells, including hematopoietic and mesenchymal stem cells [1-3] If bone marrow-derived stem cells play a role in the regeneration and repair of peripheral ... umbilical cord blood as well as in fetal and adult peripheral blood [10-12] MSCs and MSC-like cells in peripheral blood do not express hematopoietic markers or the stem cell/endothelial cell marker

Ngày tải lên: 08/08/2014, 18:20

... consume the 1 meal/d diet Separation and stimulation of peripheral blood mononuclear cells (PBMCs) The PBMCs were separated from fresh heparinized blood of healthy adult donors using Ficoll density ... meal per day (OMD) or three meals per day (TMD) isocaloric diets Serum samples and peripheral blood mononuclear cells (PBMCs) culture supernatants from subjects were analyzed for the presence of ... extend to the immune system Many stu-dies suggest that long-term CR improves several compo-nents of immune function including responses of T cells to mitogens, natural kill cell (NK) activity, cytotoxic

Ngày tải lên: 11/08/2014, 03:20

... PBMCs After resuspension in 1% FCS cell media to 1 × 106 white blood cells/ml (see above in Peripheral Blood Mononuclear Cell (PBMC) Isolation) the PBMCs were plated cell culture polystyrene flasks ... lung epithelial cells and white blood cells, we decided to investigate the role of the IFN-γ/IL-12 path-way on IP-10 secretion upon the interaction of peripheral blood mononuclear cells with two ... human epithelial cell lines Cells from a human bronchial epithelial cell line (Calu-3) and from a human alveolar epithelial cell line (A549) were used for the present studies Both cell lines were

Ngày tải lên: 12/08/2014, 15:21

... by interferon-g; NC, normal control; PBMC, peripheral blood mononuclear cells; SLE, systemic lupus erythematosus. Trang 9as regulation of immune system [10,12-18,36] To thebest of our knowledge, ... members of the IFN super-family [7,8] They are secreted by human peripheral blood mononuclear cells (PBMC) as well as dendritic cells (DC) upon infection with viruses or stimulation with poly ... lymphocytes, and influence maturation of DC, T cell and bone marrow progenitor [20-23] Moreover, they can stimulate monocytes, natural killer (NK) and T cell migration, and modulate adhesion molecule

Ngày tải lên: 12/08/2014, 15:23

... Modulatist on peripheral blood mononuclear cell (PMNC) proliferation Discussion Stem cell therapy has become a promising therapy for certain degenerative diseases Although autologous stem cell transplantation ... chondrocytes as compared to mesenchymal stem cells from bone marrow and adipose tissue J Cell Biochem 112, 1206-1218 Zhao, Q., Ren, H., and Han, Z (2016) Mesenchymal stem cells: Immunomodulatory capability ... Keywords: ModulatistTM, mesenchymal stem cell, cytokines, immunomodulation, T cell suppression, stem cell drug, immune diseases INTRODUCTION Mesenchymal stromal cells (MSCs) are multipotent progenitors

Ngày tải lên: 16/12/2017, 00:08

... factor-alpha in bone marrow-derived mesenchymal stem cells Stem Cells 2009;27(4):942 –8. 36 Zhang T, Lee YW, Rui YF, Cheng TY, Jiang XH, Li G Bone marrow-derived mesenchymal stem cells promote ... maintains stemness through decreasing ROS generation in mesenchymal stem cells Cell Death Dis 2013;4:e844. 25 Alhadlaq A, Mao JJ Mesenchymal stem cells: isolation and therapeutics. Stem Cells Dev ... of tumor cells and non-tumorous, supportive cells that are com-monly termed “tumor stroma” Tumor stroma include cancer-associated fibroblasts, bone marrow-derived mes-enchymal stem cells (BM-MSCs),

Ngày tải lên: 23/07/2020, 03:03

... cartilage has poor regenerative capacity Since bone marrow-derived mesenchymal stem cells (BMSCs) can chondrogenically differentiate, they are promising for cell-based regeneration of damaged articular ... on chondrogenesis Methods Culturing of human fetal bone marrow-derived mesenchymal stem cells Human fetal BMSCs (#SCC7500, Lot#6890, ScienCell Research Laboratories, Carlsbad, CA, USA) were ... 1SMAD3 and SMAD4 have a more dominant role than SMAD2 in differentiation of bone marrow-derived mesenchymal stem cells Laurie M G de Kroon1,2, Roberto Narcisi2, Guus G H van den Akker1, Elly

Ngày tải lên: 04/12/2022, 16:28

... hematopoietic stem cells from the bone marrow into the bloodstream of patients with a variety of malignancies The stem cells are then harvested from the blood as a source of stem cells to be returned ... stromal cell-derived factor 1 expression by bone marrow stromal cells TNF injection decreases stromal cell-derived factor 1 expression by bone marrow stromal cells Expression of stromal cell-derived ... levels are decreased in bone marrow stromal cells and long bone samples from TNF-Tg mice to account for the increased OCP mobilization from their bone marrow to their peripheral blood SDF-1 mRNA and

Ngày tải lên: 09/08/2014, 10:23

... Guide to Stem Cell and Bone Marrow Transplantation Trang 18Page 1Introduction to Stem Cell and Bone Marrow Transplantation The pluripotent stem cells from which all committed blood cells arise ... to Stem Cell and Bone Marrow Transplantation Terry Wikle Shapiro Trang 12Antimicrobial prophylaxis 85Trang 13Page viiiChapter 4 Bone Marrow, Peripheral Blood Stem Cell, and Umbilical Cord Blood ... I.1) are located in thebone marrow space, the peripheral blood, and the blood in the umbilical cord of newborns All ofthese sites can be used as a source of bone marrow stem cells, whether for autologous

Ngày tải lên: 10/08/2014, 08:20

... EvaluationA There are numerous physiologic and psychological risks involved with bone marrow/peripheralblood stem cell donation Donors must be screened carefully to identify potential problems ... red cells due to an insufficiency of marrow stemcells (7) Impaired erythropoietin production in the kidneys, leading to insufficientstimulus of red cell production (seen in allogeneic BMT) (8) Marrow ... of stem cells that have been infusedb) Source of stem cells c) Underlying disease (particularly in autologous BMT)d) Post-transplant immunosuppression therapy Trang 20Page 49nucleated red cells

Ngày tải lên: 10/08/2014, 08:20

... least Trang 9neutropenia-2 hours Infusion generally started two to four hours after marrow/peripheral bloodstem cell (PBSC) infusion Continued until absolute neutrophil count is greater than7000 ... colony-stimulating factor glycoprotein used to stimulate earlyprogenitor cells of all three cell lineages (red cells, platelets, and white cells) Decreases period of neutropenia and thus decreases the number ... Interleukin-3 (IL-3) is used to stimulate the earliest progenitor cells of all celllineages (red cell, platelets, and white cells) May decrease the period of neutropeniaand thus decrease the

Ngày tải lên: 10/08/2014, 08:20

... numbers of stem cells, ex vivo manipulation of marrow (purging), andcryopreservation.3 3 Risk factors a) Recipient of HLA-incompatible marrow graftb) Recipient of matched unrelated donor marrow graftc) ... sibling donor recipient is thought toresult from marrow rejection by host T cells not eliminated during conditioning 9d) T-cell depletion of donor marrow to prevent GVHD also contributes to graftrejection ... See Table 5.1 for blood component therapy 2 b) Avoid invasive procedures that could result in additional blood loss, unlessabsolutely necessary c) Transfuse packed red blood cells if HCT is less

Ngày tải lên: 10/08/2014, 08:20

... determined histocompatibility differences exist between the bonemarrow recipient and the bone marrow donor b) Immunocompetent cells in the donor's marrow that can recognize the foreignhistocompatibility ... hemolytic anemia, and reduced hematopoieticprogenitor cells are seen upon examination of the bone marrow The bone marrowmay become hypoplastic Marrow fibrosis with transfusion-dependent anemia, ... GVHD.a) Initiation by donor T cells b) Persistence of circulating alloreactive T cells c) Development of autoreactive T cells d) Development of counterbalancing regulatory cells e) Development of circulating

Ngày tải lên: 10/08/2014, 08:20

... Immunological reconstruction after allogeneic marrow, autologous marrow or autologous peripheral blood stem cell transplantation In: Atkinson K, ed Clinical Bone Marrow Transplantation Cambridge, England: ... bone marrow transplantation Bone Marrow Transplant 1991;8: 159–170. 2 Bosi A, Vannucchi AM, Grosi A Serum erythropoietin levels in patients undergoing autologous bone marrow transplantation Bone ... in children after bone marrow transplant for hematological malignancies Bone Marrow Transplant 1991;8(suppl 1)2–4. Savdie E Renal complications In: Atkinson K, ed Clinical Bone Marrow Transplantation.

Ngày tải lên: 10/08/2014, 08:20

... nephrotoxicity, H/A, lethargy, tremulousness,delirium, seizures, N/V, rash, sore throat, insomnia,bone marrow depression, elevated liver enzymes levelsOintment: 5% (15 g) Adults: 200 mg PO 5 times ... tachycardia, anorexia, N/V,diarrhea, orthostatic hypotension, dizziness, edema,SLE-like syndrome, peripheral neuritis, weakness(Apresoline) Tab: 10, 25 mg Oral: 0.25–1 mg/kg 3–4 times per dInjection: ... congestive heart failure (CHF), dizziness, irregular heartbeat, orthostatic hypotension,N/V, reduced peripheral tone, stuffy nose, weakness(Trandate, Normodyne) Injection: 5 mg/mL (20 mL) Oral: Initial

Ngày tải lên: 10/08/2014, 08:20

... outlines marrow donor-recipient red blood cell incompatibility Table 8.1 Marrow Donor-Recipient Red Blood Cell incompatibility Recipient type Donor Type Transplant incompatibility Transfuse: red blood ... Transfuse: red blood cells Plasma Trang 111 The ABO and Rh blood types of both the patient and the bone marrow donor areimportant consideration, in red cell transfusion of the marrow transplant ... nonhemolytic febrilereactions II Blood and Blood Products A Red blood cell transfusions 1 Indications a) Cytomegalovirus (CMV)-negative blood products: CMV-negative red cells areonly given to CMV-negative

Ngày tải lên: 10/08/2014, 08:20

... simvastatin on the cell viability of bone marrowderived MSCs in vitro To examine whether simvastatin has anti-apoptotic effect on bone marrow- derived MSCs under hypoxia stress, cells viability was ... of bone marrow- derived MSCs Flow cytometric analyses of bone marrow- derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 Figure Effect of simvastatin and bone ... of bone marrow- derived stem cells in the treatment of ischemic hindlimb Overall, this study demonstrates more pronounced angiogenic response, decreased muscle cell apoptosis and improved blood...

Ngày tải lên: 10/08/2014, 05:21

... the stem cells and/or attract the endogenous or exogenous stem cells (endogenous from the bone marrow and exogenous by multiple intra-articular injection of the expanded autologous stem cells) ... (265, 266), or introducing new cell sources like debrided waste chondrocytes, Bone Marrow derived Mesenchymal Stem Cells (BM MSCs), or any combination of these cells (29, 214, 215, 267, 268) ... the bone marrow and expanded the cells the same as Wakitani’s approach however, we prepared the cell sheets (by useing Ascorbic acid) instead of cell suspension Seventy milliliters of venous blood...

Ngày tải lên: 10/09/2015, 09:01