Tiêu chuẩn iso 11024 2 1998

A Reference number ISO 1 1 024 2 1 998(E) INTERNATIONAL STANDARD ISO 1 1 024 2 First edition 1 998 1 2 01 Essential oils — General guidance on chromatographic profiles — Part 2 Uti l ization of chroma[.]

Refere ce n mb r

ISO 1 0 4-2:19 8(E)

First e itio

19 8-12-01

Huies es entieles — Directives génér ales con ernant les profis

c r omatogra hiq es —

Par tie 2: Uti sation des pr ofis c romatogra hiq es des éc anti on

d’h ies es entieles

© ISO 19 8

Al r i hts r es r ve Unle s oth r w is s e ifie , n p r t of this p blc tio ma b r epr od c d

or uti z d in a y for m or b a y me n , ele tr onic or me h nic l in lu in p oto o yin a d

micr ofim, with ut p r mis io in w ritin from th p bls e

Inter natio al Org niz tio for Sta d r diz tio

Ca e p stale 5 • C -121 Ge è e 2 • Switz r l n

Intern t is @is c

Pr i te in Switz r l n

i

1 Sc pe 1

2 Normativ refere c s 1

3 Terms a d d finitio s 1

4 Prin iple 2

5 Sta d rd matc in s lutio 2

6 Ap aratu 2

7 Performa c of the ap aratu 3

8 Preparatio of te t s mple 4

9 Lo atio of th repre e tativ a d c ara teristic c mpo e ts 4

10 Ch c in th c n e tratio of th repre e tativ a d c ara teristic c mpon nts 5

1 Expre sio of re ults 5

12 Te t report 5

Forew ord

ISO ( he International Organization for Stan ardization) is a worldw ide

federation of national stan ar ds b dies (ISO memb r b dies) The work of

pre arin International Stan ard is normaly car ried out thr ou h ISO

tec nical commite s Eac memb r b d interested in a s bject for whic

a tec nical commite has b en esta ls ed has the rig t to b re r esented

on that commite Inter national organization , governmental an non

-gover nmental, in laison with ISO, also tak e p rt in the work ISO

cola or ates closely w ith the International Electr otec nical Commis ion

(IEC) on al mater s of electr otec nical stan ar dization

International Stan ar ds ar e drafed in ac ordan e w ith the r ules given in

the ISO/IEC Dir ectives, Par t 3

Draf Inter national Stan ar ds ado ted by the tec nical commite s are

cir culated to the memb r b dies for votin Publcation as an International

Stan ar d req ires a proval by at le st 7 % of the memb r b dies castin

a vote

International Stan ard ISO 1 0 4-2 was pre ared by T ec nical Commite

ISO/TC 5 , Es ential ois

International Stan ard ISO 1 0 4 con ists of the folowin p rts, u der the

general title Es ential ois — General g idan e on c romatogra hic

pr ofies:

— Part 1: Pre aration of c romatogra hic profies for presentation in

stan ard

— Part 2: Uti zation of c romatogra hic profies of samples of es ential

ois

This p r t of ISO 1 0 4 des rib s gener al g idelnes on the deter mination of the complan e of a c romatogra hic

profie of a sample of es ential oi u der examination w ith the r efer en e c romatogra hic pr ofie given in the

stan ar d for that oi

NOT Refer also to ISO 1 0 4-1

1)

The folow in normative doc ments contain provision w hic , throu h refer en e in this text, con titute provision of

this p rt of ISO 1 0 4 For dated r efer en es, s bseq ent amen ments to, or revision of, an of these publcation

do not a ply However, p rties to agre ments b sed on this p rt of ISO 1 0 4 ar e en ouraged to in estigate the

p s ibi ty of a plyin the most recent edition of the normative doc ments in icated b low For u dated

referen es, the latest edition of the normative doc ment referr ed to a ples Memb r s of ISO an IEC maintain

r egister s of c r r ently vald Inter national Stan ar ds

ISO 3 6, Es ent ia l ois — Pr epa r a t ion of t est sa mples

ISO 7 0 , Es ent ia l ois — A na ly is by ga s c r oma t ogr a ph on c a pi a r y column — Gener a l met hod

For t he purposes of t his pa rt of ISO 1 0 4, t he f olowin t erms a nd def init ion a pply

3.1

comp nents pr esent in al of the samples of the es ential oi in olv ed, whether major or minor ones

EX MPLE Ger anyl formate, isome th n , citr on elal, gera iol, etc in th es e tial oi of gera ium

1)

ISO 1 0 4-1, Es e t ial ois — Ge er a l guida nce o c hromat ograp ic prof iles — Pa rt 1: Prepa ra t io of c hromat ogra phic prof iles

f or prese ta t io in st a nda rds

3.2

c ara teristic c mpo e ts

one or mor e r epr esentative comp nents, the con entr ation of whic is c ar acter i tic for a given es ential oi

Gu ia-6,9-die e is pr ese t in traces in th Afr ica ger anium a d pr ese t in high r co ce tr atio s in th Bo rb n gera ium

10-Epi-gamma-e d smol is a se t in th Bo rb n ger anium a d pr ese t in th Africa gera ium

Camp or is prese t in q a tities of les th n 0,5 % in la e d r

3.3

gra hical re resentation o tained by injection into the c romatogr aph of a sample of an es ential oi con ider ed to

b r epr esentative of pr od ction, together w ith the o er atin con ition u der w hic it has b en o tained

NOT Th chromatogramis for informatio o ly

3.4

lst of comp nents selected amon the re resentative an c aracteristic comp nents of an es ential oi,

ac omp nied, for e c of them, by con entr ation lmits an , p s ibly, by the r atios b twe n these con entr ation

A sample of the es ential oi u der test is analy ed by gas c r omatogr aph on ca i ar y column

Those p ak s con idered to b re resentative an c aracteristic of the es ential oi are located on the

c romatogr am o tained They ar e comp red with those in icated in the clau e "Chromatogra hic profie" of the

stan ar d sp cific to the es ential oi b in stu ied

Usin the r es lts o tained dir ectly fr om the data-pr oces in s stem, the complan e of the minimum an maximum

con entration (or of the con entration r atios) of these re resentative an c ar acter i tic comp nents are verified

with the lmits fixed in the stan ar d sp cific to the es ential oi b in stu ied

Folowin the r ecommen ation of ISO 7 0 , pre are a stan ar d matc in solution by mixin with 1 ml of hexane

the referen e s bstan es corr esp n in to the re resentative an c aracteristic comp nents in icated in the clau e

"Chr omatogr aphic pr ofie" of the stan ar d con er nin the es ential oi b in stu ied

Chec that the r efer en e s bstan es ar e s ficiently pur e for c r omatogr aph

6.1 Ga c romato raph, pr ovided with splt injecto ca i ar y column

6.2 Flame io izatio d te tor

6.3 Data-pro e sin s stem (integr ato calc lato etc.), complyin with the in ication given in ISO 7 0

Ver ify the p r for man e of the a p r atu u in the test des r ib d in clau e 7

Set up the as embly compr i in the c r omatogr aph an the data-pr oces in s stem (6.3) in s c a man er that

p r mits the cor rect resolution an total elution of al volatie comp nents of the es ential oi, an the

c romatogr aphic profie o tained with the test mixture defined in Ta le 1 confor ms to the c romatogra hic profie

defined by the inter la or ator y test (se T able 2)

7.2 Pre aration a d c mposition of the sta dard te t mixture

Pr ep r e a stan ar d test mixtur e as in icated in Ta le 1

Table 1 — Compositio of th sta d rd te t mixture

Ch mic l n me

CAS a

EINECS

b

%

1,8-Cine le

(eu alyptol)

n-Decanal

c

a

Ch mical Abstr act Service Registr atio No

b

Registr atio No of th Eur op a In e tor y of existing commer cialy a aia le ch mical su sta ces

c

Freshly disti e a d/or ch micaly sta i ze

Chec the pu ty of e c of the comp nents by gas c r omatogr aph an by the u ual ph sico-c emical method

Store the mixtur e in ful se led b tles, s eltered fr om the lg t, an at a temp rature b twe n − ° C an + °C

Un er these con ition , the test mixtur e may b stor ed at le st a ye r

Car ry out the c romatogra hic analy is of the test mixture by injectin the later u der the u ual o er atin

con ition for es ential ois in pr actice in the la or ator y

7.4 Re ults

The r es lts ar e o tained dir ectly fr om the data-pr oces in s stem

The o tained data, expr es ed as ar ea p r cent, s al b within the lmits giv en in ta le 2

Table 2 — Chromato raphic profie of th sta d rd te t mixture

%

%

For the p a of the n-decanal, the sig al-o-noise r atio s al b gr eater than 10 T his r atio may b calc lated as

folows:

— sig al is the heig t of the p ak of decanal;

— noise is half of the diferen e b tw een the maximum an the minimum sig al values in the a sen e of a p ak

for 3 s

Comply with the method sp cified in ISO 3 6

L cate on the c romatogr am of the es ential oi b in stu ied, those p ak s whic cor resp n to the re resentative

an c ar acter i tic comp nents in icated in the r efer en e c r omatogr aphic pr ofie

This may b car r ied out in difer ent way , for example, u in one or mor e of the folow in method :

 comp rison with a typical c romatogr am o tained with a c r omatogra hic column havin the same

comp sition;

 u e of ad ition ;

 u e of r etention in ex;

 coupln with a mas sp ctr omete

Alter natively, u e a stan ar d matc in solution, as folows

Inject the stan ard matc in solution Chromatogr am A is o tained Identify the p ak s o tained as a fu ction of

their order of elution an of their ar ea, whic s al cor resp n a pr oximately to the con entration u ed for the

pr ep r ation of the stan ar d matc in solution

NOT Th or ders of elutio ar e giv n by r ea ing fr om typical chr omatograms o tain d using difer ent typ s of columns

Inject, u der the same proced r al con ition , the sample of es ential oi whose q alty is to b c eck ed

Chr omatogr am B is o tained

Usin the retention times re d on c r omatogr am A , locate on c r omatogram B the p ak s cor esp n in to the

re resentative an c aracteristic comp nents Al the re resentative comp nents s al b present in the sample of

es ential oi b in stu ied

Usin the infor mation s p led by the data s stem cor r esp n in to c r omatogr am B, method of deter mination by

the p a ar ea normalzation method (inter nal normalzation method in ac ordan e with ISO 7 0 ), c eck that the

con entration (as umed to b eq ivalent to the p a are p r centages tak en into con ideration) or con entration

ratios ar e in lu ed b twe n the minimum an maximum values sp cified in the clau e "Chromatogra hic pr ofie" of

the stan ar d sp cific to the es ential oi b in stu ied

The c romatogra hic profie is expres ed as a lst of the re resentative an c aracter i tic comp nents fou d in the

sample of es ential oi b in stu ied, ac omp nied by their con entr ation w hic have b en as es ed by the

nor malzation method Ratios of these con entr ation may also b calc lated

The test re or t s al sp cify the method u ed an the res lts o tained It s al also mention al o eratin con ition

not sp cified in this p rt of ISO 1 0 4, or r egar ded as o tional, together with detais of an in idents w hic may

have influen ed the test r es lt

The test r ep r t s al in lu e al infor mation neces ar y for the complete identification of the sample

ICS 71.100.6

De criptors: ois, e s ntial ois, c emic l a aly is, c r omato r ap ic a aly is, g s p a e c r omato r ap y, g n ral c n itio s

Pr i e b s d o 5 p g s