yield choice of culture dish and cell seeding density

... 20,000, 50,000 and 400,000 cells/cm2in expansion medium overnight We used the seeding density of 8000 cells/cm2 as a control in this experiment, since this seeding density is often used for expansion ... cell seeding densities regarding gene expression of VEGFA, TGFB, TNFA, IDO and PTGS2 The addition of TNFα and IFNγ to the culture medium increased the expression of TNFA, IDO, PTGS2, VEGFA, and ... affected by cell seeding density In contrast, VEGFA se-cretion increased with increasing seeding density in the con-trol condition from 34.7 ± 15 pg/ml at seeding density of 8000 cells/cm2to...

Ngày tải lên: 24/11/2022, 17:54

... Trang 6Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in A549 cells cocultured with MT-2 cellsFigure 3 Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 ... expression of cytokines, chemokines and cell adhesion molecule in A549 cells cocultured with MT-2 cells Figure 2 Induction of expression of cytokines, chemokines and cell adhesion molecule in A549 cells ... of A549 and NCI-H292 cells at 3 and 5 days of culture contained no viable MT-2 cells As shown in Figure 1, A549 and NCI-H292 cells cocultured with MT-2 cells showed strong expression of Tax mRNA...

Ngày tải lên: 13/08/2014, 05:21

... to those of urbanized Kampala and the central region This could be due to the linkages of culture, insecurity and HIV We explored community perceptions of HIV and AIDS as a problem and its inter-linkage ... Ministry of Health (MOH) (Uganda) and ORC Macro: Uganda HIV/AIDS Sero-behavioural Survey 2004-2005 Calverton, Maryland,USA: Ministry of Health and ORC Macro; 2006, 1-201. 3 WHO: Health and mortality ... women and girls were victims of sexual abuse due to war than men Through focus group discussions and interviews, women and girls were sighted as victims of rape and defilement by rebels and soldiers...

Ngày tải lên: 13/08/2014, 15:21

... for the choice of location of individuals and the resulting relationship between growth and inequality The details of the technologies and preferences are given below The technology of output ... purpose of the Content Anyopinions and views expressed in this publication are the opinions and views of theauthors, and are not the views of or endorsed by Taylor & Francis The accuracy of the ... Effects of heterogeneity on labour supply, choice of location and growth In this section, we analyze the effects of heterogeneity formalized through differences in the marginal disutility of work and...

Ngày tải lên: 16/12/2017, 11:18

... Information Systems (MIS) and Science of Managing(ICE) have not been the choice of many students On the author’s point of view andknowledge, MIS and ICE are those that demands lots of skills considering ... consideration of the students alongwith some matters of what affected the students’ choice of major Trang 101.3 Students’ agreement on factors affecting their choice of major6 Back-up choice in applying ... examines 100 random freshman students (K16) of International School ofVietnam National University Besides respondents’ profiles, this report looks into thereasons behind students’ major choice and future...

Ngày tải lên: 29/07/2020, 17:06

... optical density (OD) of the culture was adjusted to approximately 1.17 at 600 nm, corresponding to a density of 2.1x 109 cfu/ml [16] Mannan oligosaccharide extraction and purification Aliquot of ... extract and 2% peptone at pH6.0 was used to study the effect of inoculum size on MOS production The flasks were inoculated with inoculum range from 1% to 5% of W anomalus of cell density 2.1x109 cells/ ... Environmental factors and specific culture conditions can dramatically impact cell wall oligosaccharide production in terms of yield as well as the size and chemical composition of the saccharides...

Ngày tải lên: 06/08/2020, 01:36

... The size (width and length) of spores was measuredon30conidia of each species randomly selected using the graduated dial of the ocular of a light microscope and the number of cells per conidia ... water on 10-days old culture in Petri dishes The concentration of spores (number of conidia/ml of suspension) was assessed for each culture medium and temperature from a drop of conidia suspension ... characteristics of mycelium and conidia Microscopic observations of mycelium and conidia of each fungus on each culture medium and temperature was made using a Markham, ON, Canada) and photographs...

Ngày tải lên: 05/11/2020, 04:39

... Chart 3, all of the students (100%) chose role-playing, games, Trang 2819Trang 29Problem solving was the choice of 75% of the students And Drama and projects received only 10% and 20% of the students ... analysis of the data collected from the survey questionnaires and interviews is at the end of the part. II.1 Background of the study II.1.1 An overview of the research site Thanh Hoa University of Culture, ... Culture, Sports and Tourism is newly established on basis ofupgrading Thanh Hoa College of Culture and Arts When the school was the college, its dutywas to train in the fields of Art, Music, Culture...

Ngày tải lên: 08/11/2020, 14:51

... Trang 9LIST OF TABLES AND DIAGRAM TABLES Page No Table 3.1: Gender and age of second-year students 33 Table 3.2: Gender and age of graduates 33 Table 3.3: Gender and age of recruiters ... content and language performance Meanwhile, the writing test is conducted once in the middle of the course and in the form of paper-and-pencil The score of each course is an average of the oral and ... significance of the study, the scope of the study and the organization of the study This chapter also provides a description of the English courses of the tour guide major at NTCCAT with the purpose of...

Ngày tải lên: 23/05/2021, 22:19

... division of Thomson Learning All rights reserved. 12-14Types of Organizational Culture Degree of Environmental Turbulence Competitive Culture Adaptive Culture Bureaucratic Culture Cooperative Culture ... division of Thomson Learning All rights reserved. 12-2Chapter 12 Leadership of Culture, Diversity, and the Trang 3Chapter 12 Learning Outcomes• The power of culture. • Distinguish between a weak and ... understandings, and ways of thinking that is shared by members of an organization and taught to new members cuu duong than cong com Trang 6Copyright ©2004 by South-Western,a division of Thomson...

Ngày tải lên: 28/05/2021, 15:39

... Comparison of values of T 0 and sizes of G0 between the Prim Trajectory method and the proposed 2nd MST-based OTE algorithm on machine 1 78 4-13 Comparison of values of T 0 and sizes of G0 between ... Comparison of required memory sizes of the previous similarity-based, MST -based methods and the proposed Cell-MST-based method 68 4-6 Comparison of values of T 0, sizes of G0 the Prim Trajectory and ... on a limited memory computer (Cell-MST-based and Weighted Cell-MST-based) The Cell-RDOS algorithm is a combination of cell-based algorithms and a revised version of the ranking-based outlier detection...

Ngày tải lên: 11/07/2021, 16:37

... develop, classroom talk and discussion can be directed toward the goals of exploring ideas Trang 20and facts found in texts and in the subsequent development of thought and deepening of knowledge Gillian ... discussion, and expression of ideas, experiences and opinions across the whole curriculum This will involve a range of experiences which the size and type of audience, the situation and purpose ... respect and care about students as individuals and hope to take into account their choices of learning and social style, some teachers may be reluctant to address this issue of shyness and hence,...

Ngày tải lên: 09/09/2021, 20:56

... interactions Concepts of Culture in Psychology Although psychologists often work to examine the role of culture in the genesis of human activity, culture and social context are often treated Psychology ... con-stitution of human action and meaning (Cole, 1996; Harre & Gillett, the particularities of culture and context Nonetheless, in some versions ana-lysis of individuals and culture (Bidell ... object, self and other, psyche and culture, person and context, figure and ground, practitioner and practice, live together, require each other, and dynamically, dialect-ically, and jointly make...

Ngày tải lên: 12/10/2022, 08:50

... expression in co-cultures of the relay and receiver cells (Supplementary Fig 16a) Supplementary Fig 16b shows electronic induction of cell fluorescence of co-cultures over time, the average of which correlates ... Electronic control of cell-to-cell communication (a) Schematic of electronic control of cell-to-cell communication Electronic signals modulating Pyo and Fcn (R) to Fcn (O) result in LuxI-laa and AHL production ... which the cells were cultured a further 15 min before addition of RNA later Cells were treated as indicated and B2  108total cells were washed in equal volume of PBS and then re-suspended and stored...

Ngày tải lên: 24/11/2022, 17:42

... samples[10],thelevelsofsixflavonoidsandfourphenolicacids havebeensimultaneouslydetermined[11],andcell-wallbound phenolicshavebeenanalyzed[12].Becausethesecompoundsare sodiverseandoftenhighlydecorated,themajorityofthe ... ofthemachievedthesimultaneousdetectionandquantificationof flavonoids,andotherplantphenolics,suchasphenolicaldehydes andalcoholsthatareimportantintermediariesandcomponentsof lignin This study describes the development of a novel ... otherphenoliccompounds,includingacids,aldehydes,andalcohols.Thechromatographicseparation wasperformedin10min,allowingfortheresolutionofisomerssuchas3-,4-,and5-chlorogenicacids, 4-hydroxybenzoicandsalicylicacids,isoorientinandorientin,andluteolinandkaempferol.The...

Ngày tải lên: 31/12/2022, 09:58

... mixtures were spotted onto PEI cellulose sheets with lL of nonradioactive tracer (100 lg of 5-FU and 100 lg of uridine for the UP assay mixture; 100 lg of 5-FU and 100 lg of deoxyuridine for the TP ... tests of wild-type and upp-1(jg1) worms Fig S2 Lifespan tests of wild-type worms and three upp-1 mutants Fig S3 Sequence alignments of C elegans UPP-1 and human UPP-1 Fig S4 Sequence alignments of ... reversible phosphorolysis of uridine, yielding uracil and Rib1P, and is an important enzyme in the pyrimidine salvage pathway Human UP and TP each exhibit both uridine and thymidine phosphorylase...

Ngày tải lên: 30/03/2014, 01:20

... activation of MIG-17 ⁄ ADAMTS S Ihara and K Nishiwaki staining of cross-sections of L3 larvae with antibody to GFP revealed signals in the cytoplasm of muscle cells, the source of MIG-17 expression, and ... detected a faint band of the MIG-17–6His proform and an additional lower molecular weight band in worm lysates (Fig 3B) Monoclonal specifically recognized the activated forms of MIG-17–GFP and MIG-17–6His ... proform localizes to the surfaces of the gonad, intestine and hypodermis corresponding to the basement membranes [17] To determine the sites of activation of MIG-17 and tissue distribution of...

Ngày tải lên: 30/03/2014, 04:20

... standard Z-score (l - m)/s where l is the observed number of links in the subnetwork, and m and s are the mean and standard deviation of the number of links across 1,000 random collections of ... Jeff Wrana, and Brenda Andrews for helpful comments on the work and the manuscript We thank Jason Moffat for efforts at the proof -of- principle stage and thank Theresa Stiernagle and the C elegans ... levels of agreement The significance of the LOFA score was assessed by generating million random LOFA values We first constructed a random dataset in which the genes associated with loss -of- function...

Ngày tải lên: 06/08/2014, 18:21

... embryonic cell cultures allow the collection of cells at early stages of development, methods for the separation, culture and collection of fully developed tissues have not been established and might ... patterns of subsets of neurons, in combination with analyses of neural functions, might provide insights into understanding the distinct roles of cells within the neural network reports Materials and ... instead of FITC Forty-six wild type and 56 che-2::PABP worms at L4 to young adult were observed Cells were identified by their positions and the percentage of stained cells was scored Responses of...

Ngày tải lên: 14/08/2014, 14:21

... this time period in the context of the rate of change of coding genes, C elegans and C briggsae only share about 60% of their genes as 1:1 orthologs, and a full 10% of genes encoded in either genome ... evidence for the redundant functions of duplicated genes comes from the analysis of lossof-function phenotypes of single genes; in both yeast and worms, inactivation of a duplicated gene is less likely ... into each well of a 96-well flat-bottom plate and 40 µl of the resuspended bacterial cultures were added For combinatorial RNAi feeding experiments, resuspended saturated cultures of different...

Ngày tải lên: 14/08/2014, 17:22