... antimicrobial peptide candidates for both human and nonhuman therapeutants from genomic sequences and will aid in understanding the evolution and transcriptional regulation of expression of these ... 1Identification, structure and differential expression of novelpleurocidins clustered on the genome of the winter flounder, Susan E Douglas, Aleksander Patrzykat, Jennifer Pytyck and Jeffrey W Gallant ... PCR-amplified bands corresponding to pleurocidins WF1, WF2, WF3 and WF4 [5] Positively hybridizing clones were picked and replated until 100% purity was achieved and mapped using BamHI, SstI, XhoI and EcoRI
Ngày tải lên: 31/03/2014, 07:20
structure and written
... section, 2 the Structure and Written Expression section 3 the Reading Comprehension section ♥ In addition to good listening skills, you need to have solid skills in grammar, vocabulary, and reading. ... A and Part B is that in Part A you must choose what is correct, in Part B you must choose what is wrong. Trang 4 A B C DTrang 5The topics of the sentences in the Structure and Written Expression ... the answer is (B) b Verb and Verb Phrases Trang 9Structure 1: Form and Function The earth spins around ……that connects the geographic North and South Poles (A) the image and line (B) imagined the
Ngày tải lên: 04/12/2016, 12:21
... Zea m 1 and Cyn d 1, a second consisting of Lol p 3, Dag g 3, Lol p 1, Phl p 1, Ory s 1 and Tri a 3 and a third cluster including Lol p 2, Hol l 1, Phl p 2 and Pha a 1 Although Phl p 1 and Phl ... of virus and by expression for various periods Aliquots of the culture sup-ernatants and cell pellets were analyzed by SDS⁄ PAGE and immunoblotting with a rabbit anti-Phl p 1 antiserum and a monoclonal ... Prevalence of atopy and pollinosis in the adult population of Switzerland (SAPALDIA study) Swiss Study on Air Pollution and Lung Diseases in Adults Int Arch Allergy Immunol 106, 149–156 3 Andersson K
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
... three antiparallel strands (1, 2 and 7) and strand 6, which is parallel to strand 2 b-Strands are located between the four a-helices (a1, 278–294; a2, 305–313; a4, 374–381; and a5, 397–405), with ... ele-ments: b-strands (b3, 329–335; b4, 339–344; and b5, 367–372) and a distorted a-helix (a3, 348–356) (Fig 3B,C) The three b-strands of the minidomain are all antiparallel, and form a single b-structure ... structure of the protein core (resi-dues 277–328 and 373–413) in solution and in the solid state, and indicates their similarity A superposition of the C-domain NMR structure on the crystal structure
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf
... Therefore, we present here the apo structure of S aureusEF-G Overall structure and comparison with previous EF-G structures All five domains of S aureus EF-G are ordered in our structure The overall conformation ... C Fig 1 EF-G structure (A) Overall structure and structural domains of S aureus EF-G (PDB 2xex) The switch regions are shown in black, with switch II facing domains II and III, and switch I behind ... 2xex, magenta) and T thermophilus (PDB 1elo [13], grey) Superposition is based on domains I and II. Trang 4T thermophilusEF-G structures, wild type and variousmutants, domains III, IV and V display
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
... three-dimensional structure, and catalytic mechanism [5,6] Family 18 and family 19 glycoside hydrolases both contain chitin-ases Members of the two families have very different three-dimensional structures and ... with respect to its catalytic properties and crystal structure The crystal structures of ChiG and ChiC [18] permitted structural comparison between bacterial and plant enzymes, which provided a structural ... extended towards Cys183 in ChiC (and in the plant enzymes) Compared to the barley enzyme, ChiC and ChiG lack three loops (A, B and C) and a C-terminal exten-sion (Figs 1 and 4B) In addition, one other
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx
... of these have been purified and characterized as alkaline [12] and acidic RNases [13], and a third poly(U)- and poly(C)-specific ribo-nuclease has also been isolated and characterized [14,15] In ... Trang 2development, and control of reproductive maturationand sexual behaviour in adults [6] Drosophila RNase H1 is one of the best-studied insect ribonucleases [7], and its expression is essential ... vertical black and grey bars represent the 0.9 and 1.5 kb tran-scripts, respectively Error bars denote the standard error of the mean for three independent measurements. Trang 3epidermis and intestine
Ngày tải lên: 23/03/2014, 06:20
Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf
... at 25C , 30 Cand 35Cin order to identify scalar and dipolar connectivities among heme and amino acid residues Spectral widths of 13 kHz and mixing times of 80 ms for NOESY and 50 ms for TOCSY ... the crystal structure of Pe metHbH 2 O [16]and as confirmed herein by solution 1 H NMR of Pe metMbCN Proximal and distal residues are shown as rectangles and circles, respectively, and arrows connecting ... H-bonds to ligands and/or sterically interfere with ligand binding [6] Among the extensively studied mono-meric mammalian Mbs, the key residues have been identi-fied at positions E11 and E7 (generally
Ngày tải lên: 23/03/2014, 17:22
Storage Structure and Relationships pdf
... database can be created with a standard block size and up to four nonstandard block sizes. • Block sizes can have any power-of-two value between 2 KB and 32 KB. Trang 12Standard Block Size• Set at ... 1Storage Structure and Relationships Trang 2After completing this lesson, you should be able to do the following: • Describe the logical structure of the database • List the segment types and their ... • List the keywords that control block space usage • Obtain storage structure information Trang 3Storage and Relationship StructureRB Seg S_DEPT Data Seg S_EMP Data Seg Temp Temp Seg S_EMP FIRST_N
Ngày tải lên: 29/03/2014, 16:20
Báo cáo khoa học: Functional aspects of the solution structure and dynamics of PAF – a highly-stable antifungal protein from Penicillium chrysogenum pdf
... Asn12 and Lys17 (region 2) and between Lys34 and Asp39 (PAF) and Lys31 and Asp36 (AFP) (region 3). Two conserved GlyLys motifs are repeated in the structure, the glycines (i.e. Gly5 from b1 and ... adjacent Lys9 and Lys39. Similarly, Asp23 stabilizes the juxtaposed Lys15 and Lys17 side chains. The hydrophobic strand b4 is located between Cys43 and Asp46 and, as a central strand of sheet ... [14]. The 3D molecular structure of PAF consists of five b-strands connected by three small loops involving b-turn motifs (loops 1, 2 and 4) and the large loop 3 (Fig. 9). The b-strands create two orthogonally
Ngày tải lên: 29/03/2014, 23:20
Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 pdf
... of r2and sum of squares ( · 10)3) were as follows: citrate, competitive 0.92 and 518 versus noncompetitive 0.95 and 351; isocitrate, competitive 0.98 and 121 versus noncompetitive 0.99 and 58. ... EFGH and IJKL tetramers, and residues 89–104, which form a helix–loop structure on the surface Deviations between monomers of the two structures are in the same range as within independent structures ... Overall structure The variation in size and the observed amino acid modification between PpCMLE and ArCMLE sugges-ted important differences between the two enzymes Therefore, the crystal structure
Ngày tải lên: 30/03/2014, 10:20
Báo cáo khoa học: Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide pot
... H2O and 2H2O was adjusted to pH 5.5 The standard COSY, TOCSY as well as 100 and 200 ms mixing time NOESY spectra were accumulated, processed byNMRPIPE[21] and analyzed byX-EASYprogram [22] Structure ... bold letters and that of signal peptide is underlined The final DNA products were digested with BamHI and EcoRI and inserted into the vector pFastBac, yielding, respectively, pFastBacSPx and pFastBacPx ... Trang 1Poneratoxin, a neurotoxin from ant venomStructure and expression in insect cells and construction of a bio-insecticide Ewa Szolajska1, Jaroslaw Poznanski1,
Ngày tải lên: 30/03/2014, 13:20
Báo cáo khoa học: Calcium modulates endopeptidase 24.15 (EC 3.4.24.15) membrane association, secondary structure and substrate specificity pdf
... Trang 1membrane association, secondary structure and substrate specificity Vitor Oliveira1, Paula A G Garrido2, Claudia C Rodrigues2, Alison Colquhoun2, Leandro M Castro2, Paulo C Almeida3, Claudio ... with the wild-type enzyme These data and the model of the enzyme’s structure as determined by X-ray diffraction suggest that D93 is located at the enzyme surface and is consistent with membrane association ... optical sections and averaged over 32 scans per frame They were then processed using the Carl Zeiss CLSM software (version 3.1) and stored on Jazz disks for further retrieval and editing Final
Ngày tải lên: 30/03/2014, 16:20
báo cáo hóa học: " Interleukin-1alpha expression precedes IL-1beta after ischemic brain injury and is localised to areas of focal neuronal loss and penumbral tissues" pdf
... 1This Provisional PDF corresponds to the article as it appeared upon acceptance Fully formattedPDF and full text (HTML) versions will be made available soon Interleukin-1alpha expression precedes ... immune studies and analysis KJK contributed to the design and execution of the surgical studies GLC contributed experimentally DB contributed to design and analysis of the study and wrote the ... design and analysis of the study and wrote the manuscript All authors read and approved the final manuscript Acknowledgements Trang 9This work was funded by the Wellcome Trust (DB, NML, GLC), and
Ngày tải lên: 19/06/2014, 22:20
Báo cáo lâm nghiệp: "Structure and development of forest stands on permanent research plots in the Krkonoše Mts" pdf
... was done and prediction of development after 25 and 50 years performed Th e forest stand diversity according to tree spe-cies composition and representation, horizontal and vertical structure ... forest stands develop within the small developmental cycle Trang 3Beech standsNatural beech stands in the Krkonoše Mts are marked by high age heterogeneity, low volume and structure variability and ... the ageing stand Table 2 presents growth variables after spontaneous development for the whole stand and beech as dominant tree species Mixed stands Mixed forest stands of beech, fi r and spruce
Ngày tải lên: 07/08/2014, 10:21
Báo cáo y học: "Sleep structure and sleepiness in chronic fatigue syndrome with or without coexisting fibromyalgia" pdf
... Methods We compared sleep structures and subjective scores on visual analog scales for sleepiness and fatigue in CFS patients with or without coexisting fibromyalgia (n = 12 and 14, respectively) ... symptoms and had poorer sleep efficiencies and shorter runs of sleep than both controls and patients in the less sleepy group; patients in the less sleepy group reported reduced fatigue and pain ... illness, 1 patient and 2 controls; current depression, 5 patients; obesity, 1 patient; abnormal labs, 1 patient and 5 controls; moved or no longer interested, 3 patients and 2 controls; and technical
Ngày tải lên: 09/08/2014, 10:23
The intellectual structure and substance of the knowledge utilization field: A longitudinal author co-citation analysis, 1945 to 2004 pdf
... Rogers, and strongly linked to him and to Griliches, are geographers Torsten Hägerstrand and Law-rence Brown, who researched the spatial aspects of diffu-sion theory [88-90] Hägerstrand also ... (publications) and theactual processes of forming knowledge [106] Merton iscited in this decade for the first and revised editions of hisbook: "Social Theory and Social Structure" [107,108], andfor ... research, such as communication and agricultural innovation [78,79] Katz' and Rogers' nodes are close to and strongly linked to the nodes of sociologists James S Coleman and Herbert Menzel, who worked
Ngày tải lên: 11/08/2014, 05:21
báo cáo khoa học: "Structure and expression of the maize (Zea mays L.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants" pot
... at least two genes ZmSUN1 and ZmSUN2 are CCSD-type and are most closely related to plant SUN-domain homologs AtSUN1, AtSUN2, and OsSad1 ZmSUN3, 4, and 5 are PM3-type and probably represent a previously ... withAtSUN1 and AtSUN2 firmly establish that these pro-teins reside in the NE like their animal and fungalcounterparts [17-19] SUN-Domain Proteins and Meiotic Chromosome BehaviorSome animal and fungal ... C-terminal SUN domain(CCSD) and plant-prevalent mid-SUN 3 transmembrane(PM3) We also provide the first evidence for expressionand localization of PM3-type proteins and discuss thepossible significance
Ngày tải lên: 11/08/2014, 11:21
báo cáo khoa học: " The effect of experimental warming on leaf functional traits, leaf structure and leaf biochemistry in Arabidopsis thaliana" pdf
... climatic warming on SOD and CAT activities, contents of MDA, proline, carbohydrates and chloro-phyll of Arabidopsis thaliana leaves, and leaf micro-structure and ultramicro-structure, apart from ... photosynthesis and respiration acclimation, but leaf structure (microstruc-ture and ultrastruc(microstruc-ture) and biochemical processes were seldom focused on [1,11,14] Because leaf structure is ... distribution, and reproduction in Trang 2warming is expected to have profound effects on plantphysiology and growth, structure and function of plant populations, species distributions, and probabilities
Ngày tải lên: 11/08/2014, 11:21
báo cáo khoa học: " Ectopic expression of MdSPDS1 in sweet orange (Citrus sinensis Osbeck) reduces canker susceptibility: involvement of H2O2 production and transcriptional alteration" pdf
... analysis on the expression level of MdSPDS1 in the wild type (WT) and two transgenic lines (TG4 and TG9) (D-E) Analysis of free (D) and conjugated (E) polyamine content by HPLC in fully expanded leaves ... (WT) and transgenic line (TG9) after Xac infection Free putrescine (A), spermidine (B) and spermine (C) contents (nmol/mg FW) were analyzed in the WT and TG9 leaves sampled on 1, 2 and 3 DPI ** and ... and TG9, but the level is higher in the latter line (Figure 2C) Free and conjugated polyamine levels in the transgenic lines and WT under normal conditions Free polyamine levels of TG4, TG9 and
Ngày tải lên: 11/08/2014, 11:21
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