the dynamic type and properties

... also set or replace them from the command line with the −D command−line option to the java command For instance, the following will add the foo property to the set of system properties with a value ... list, the value for the key from the defaults set will be returned 64 Using Properties public Properties( ) public Properties( Properties defaults) Instances of the Properties class are both sized and ... keys Instead, the Properties class provides its own propertyNames() method, which combines the keys in the properties list with the keys in the defaults properties list passed into the constructor:...

Ngày tải lên: 05/10/2013, 12:20

... of the PNA strand, flanking the A24 C7 mismatch in PDwt (Scheme 1), and the corresponding AT(6–7) & TC(7–8) steps of the DNA strand (Fig 2E,F), and GA(23–24) & AT(24–25) (Fig 2G,H) steps of the ... PDmut On the other hand, stacking at the AC(6–7) step in the DNA strand of PDwt is retained during the entire simulation in spite of the large movement of C7 (Fig 2A) This occurs due to the coordinated ... 6C–F) The transition to the favoured hydrogen bond occurs as a result of movement of A24 rather than C7 (of the DNA strand) as found in PDwt and persists till the end of ns dynamics Further,...

Ngày tải lên: 07/03/2014, 21:20

... This thesis is organized into 10 chapters Chapter introduces the background of the study, the objective and research questions, the significance of the study, the contribution to the knowledge, and ... Hinduism and Islam Lastly, the character of the local economy is examined, including the nature of poverty and the livelihood of the population, the structure of the informal economy, the microenterprise ... its inception The BMT sector and its position in the Islamic banking and finance landscape are presented, as well as a description of the relations between the BMT sector and the other Islamic...

Ngày tải lên: 28/11/2015, 14:02

... extracts The properties of the enzyme purified from T thioparus were therefore studied in an effort to clarify the nature of reaction and to assign a possible role to the enzyme in the oxidation of the ... preparations The inhibition was not restored by the addition of GSH and seems, therefore, to be due to inactivation of the enzyme These metals may compete with iron on the enzyme and thus cause ... Absorption by the enzyme preparation was measured at 325 m# and compared to standards of i, 3, and parts per million copper Labile sulfide was determined by a modification of the method of FOGO AND POPOWSKY12...

Ngày tải lên: 21/12/2016, 10:51

... between the first and second scan may have contributed to the small shift in the onset temperature The endotherm was not observed during the third scan, because the sample melted at 210 °C during the ... through the center of the sample and reference sensors of the DSC cell Thermal isolation was maintained by using beryllium metal foil to seal the X-ray optical path identification and understanding ... dioxide, and other volatiles evolved, which indicates thermal degradation Thermal degradation after the melt was also indicated by exothermic behavior observed from the DSC experiments and by visual...

Ngày tải lên: 14/02/2014, 03:20

... determined by the value of an individual’s taste parameter and the standard deviation of the shock parameter There is no random component to the model of the choice of the length of stay The decision ... Comparing the Dynamic Retention Model and the Annualized Cost of Leaving Family of Models 15 The key difference from the DRM is that the ACOL is computing the maximum of the expected values rather ... advance to either the officer or the analyst The shock affects the value the individual places on staying in the military until the next decision The shock can make an individual place either a higher...

Ngày tải lên: 17/02/2014, 23:20

... reaction time, the yields of the trimer and the pentamer compared to the dimer were 34% and 30% for the wildtype, but 66% and 47% for the D392N mutant (Fig 5B) A Oligosaccharide synthesis by chitinase ... judged by the patterns of the product formation, the release of dimers, trimers and tetramers from the hexamer was considered to result from direct action of the enzymes On the other hand, the pentamer ... (GlcNAc)6, synthesized relatively to the hydrolytic product, (GlcNAc)2, were 3% and 9% for the wild -type enzyme and 11% and 12% for the D392N mutant The synthesis of (GlcNAc)8 was also detected,...

Ngày tải lên: 20/02/2014, 01:20

... acid residues with the catalytic a subunits of the two tungsten and selenocysteine-containing FDHs from Eubacterium acidaminophilum [32] and with the a-subunit of FDH from the thermophilic acetogenic ... determine the N-terminal amino acid sequence of the larger subunit using a number of different preparations of the protein The gene encoding the smaller subunit (fdh1B) was identified in the unfinished ... using the N-terminal amino acid sequence of the b-subunit as a query The sequence of 4741 bp containing fdh1A and fdh1B has been deposited with GenBank under the accession number AF489516 The amino...

Ngày tải lên: 20/02/2014, 23:20

... containing the double of NrfA subunit in respect to the NrfH subunit Nevertheless, inside these huge aggregates there are species of the type a2b2 The present findings are in agreement with the reported ... well as the former simulation as they to the experimental data The large subunit NrfA, contains the high-spin heme, and four low-spin c-hemes with gmax ¼ 3.6, 3.50, 3.2 and 2.96, while the NrfH ... cytochrome c, NrfB [22] These important developments demand the re-examination of the biochemical properties of D desulfuricans ATCC 27774 ccNiR, and its implications on the existing spectroscopic...

Ngày tải lên: 21/02/2014, 00:20

... amyloliquefaciens and the respective gene-speci®c, internal primers (Table 2) These PCR fragments were cloned into the pUC18 vector and the resulting pUC18-sip construct ligated into the PstI site of the temperature ... either 30 °C or 42 °C, the two cultures were labelled for at the indicated temperature by the addition of 35S[methionine] (50 mCiáL)1) and chased by the addition of nonradioactive methionine and ... amyloliquefaciens and the degenerative primers V1/V3 and V2/V3 or W1 and W2, led to the isolation of core DNA fragments of about 0.5 kb (lane b) and 0.4 kb (lane c), or 0.2 kb, respectively (A2/B2) The respective...

Ngày tải lên: 21/02/2014, 03:20

... between the performance of the wild -type and mutant enzymes (Table 1) The results for these three mutants were extremely puzzling; the mutations had been designed to facilitate binding of the phosphorylated ... product before the steady state is reached: the presence or absence of a burst provides information on the position of the ratelimiting step along the reaction pathway, and the amplitude of the burst ... Sigma-Aldrich Ireland Ltd (Dublin, Ireland) The agreement of the rapid reaction kinetics with the HPLC analysis suggested nearly total conversion of NAD+ into NADH in the reaction observed From the equilibrium...

Ngày tải lên: 06/03/2014, 00:20

... b sheet with the b12 of the other monomer, and towards the C-terminus, the subsequent residues, Phe182Lys186, pass through the a2 vicinity of the other monomer For the FeR dimer, the N-terminal ... present the crystal structure of DvMF avoredoxin and discuss the key residues for ligand binding and metal ion binding, based on the crystal structures Results Cloning and sequencing of the avoredoxin ... Structure of the FMN-binding region The hydrogen bonds and salt bridge that encompass the ribitol moiety and the phosphate group of FeR and M acetivorans avoredoxin are moderately and completely...

Ngày tải lên: 07/03/2014, 02:20

... is consistent with a topology where the loop is in the thylakoid lumen, and the N -and C-termini face the stroma, opposite to that of PSI-K On the other hand, the second scenario implies a stromal ... transcription and translation, or into pPS48 [20] under the control of the 35S promoter and terminator For each tagged variant and the wild -type PSAG, a cassette containing the PSAG construct flanked by the ... of the thylakoid membrane using the detergent dodecylb-d-maltoside Here, the solubilization of the complex from the membrane clearly renders the PSI-O subunit accessible to the protease but the...

Ngày tải lên: 07/03/2014, 21:20

... brings the loop into the proximity of the zinc ion and enables direct contact between Gln400 and the carbonyl oxygen of the serine substrate [12] These movements are required to position the carboxylate ... crucial for the selection and binding of tRNA [7] In the crystal structure of T thermophilus SerRS complexed with tRNASer, the N-terminal helical arm is buried between the TwC arm and the long extra ... attack of the a-phosphate of ATP Thus, according to the crystal structure, the specificity of serine recognition depends on: (a) the zinc ion, (b) the size of the active site and (c) the hydrophilic...

Ngày tải lên: 16/03/2014, 06:20

... for the eye The dashed line corresponds to the band-gap for the undoped Si-nw Fig 11 shows how the FE for the B and P codoped Si-nw changes as function of the position of the dopants within the ... HOMO and LUMO states reveal their localization within the Si-nc, in particular the HOMO state is localized on the B impurity, while the LUMO is localized on the P one [17] The presence of these ... are present in the literature They are mainly concentrated on H-passivated or pristine Si-nw and demonstrate the dependence of the energy band-gap from the wire diameter and from the surface morphology...

Ngày tải lên: 16/03/2014, 15:15

... cofactor onto the structure of the ligandfree rPAH (containing the regulatory and catalytic domains) [19] revealed that both the reduced and the oxidized cofactor interact with the N-terminal ... Superpositions of the binary and ternary complexes of hPAH onto the crystal structure of the ligand-free dimeric rPAH (PDB id code 1PHZ) [19], which contains both the regulatory and the catalytic ... either H4biopterin, L-Phe, or both ligands combined, no signicant effect of the ligands on the rate of proteolysis was observed as compared to the ligand-free enzyme (Fig 1B) In this case, the...

Ngày tải lên: 17/03/2014, 09:20

... describe the cloning and expression of the M tuberculosis tps gene in E coli, and the production of active TPS in these cells The properties of the recombinant enzyme have been determined, and are ... seems likely that under the appropriate conditions in the cell, the enzyme can catalyze either the synthesis or the degradation of trehalose Another unusual enzyme in E coli is the trehalose-6-P hydrolase ... of hyphae and did decrease the infectivity of the organism, but it was not lethal Thus, the rate of growth of the mutant at 30 °C was indistinguishable from the growth rate of the wild -type, although...

Ngày tải lên: 17/03/2014, 10:20

... sequences around the GTPbinding region, catalytic site, and Ca2+-binding region are highly homologous to the corresponding regions of the human TGase and the other invertebrate TGases (Fig 3) The eight ... Although the predicted aminoacid sequence had similarity with the sequences of mammalian TGase, the length of the cDNA was smaller than the length deduced from the molecular size of the purified ... sequence The numbers of nucleotides and amino-acid residues are shown on the left and right sides, respectively The grey background indicates the amino acid sequence determined from the CNBr...

Ngày tải lên: 17/03/2014, 23:20

... reduced the rates of shortening and growing phases by 39% and 19%, respectively, and reduced the extent of the growing and shortening phases by 60% and 65%, respectively Like several other tubulin-targeted ... counted for each set and the experiment was repeated three times The numbers of cells at the metaphase and anaphase stages of the cell cycle were calculated for both the control and curcumin-treated ... washed off with fresh media The cells were incubated without or with curcumin for and h, and then stained with PI The effect of curcumin on the kinetics of the release of the mitotic block was examined...

Ngày tải lên: 23/03/2014, 03:20

... of the catalytic rate for the wild -type (50 s)1; pH 7.0) and the catalytic rates of the H334G mutant in the absence (0.0015 s)1; pH 6.5) and the presence (0.0081 s)1; pH 7.0) of imidazole The ... to the wild -type upon the addition of the same concentration of phosphate pH profiles The pH dependences of logarithmic rates of the H334G mutant and wild -type are compared in Fig Data for the ... weakly inhibited the wild -type (Fig 2) Acetate and formate had no effect on the activity of the H334G mutant Azide, 2-methylimidazole and 2-ethylimidazole inhibited the mutant The wild -type was inhibited...

Ngày tải lên: 23/03/2014, 07:20