the crystal structure of silicon 38

... and the diatomic ligand (CO) of the Fe atom The three meshed spheres show the electron density of the bridging ligand assigned as an oxygen, the diatomic ligand (CO), and the modification of the ... that part of the sample showing the split signals in the EPR spectrum (seeFig S1b).49 Only few crystals were suitable for the high-resolution X-ray diffraction experiments The other crystals were ... in the region of the CO stretching band (at 1951 cm− 1) without a counterpart at the CN− vibrational region For this band, no clear assign-ment was achieved The overall structure The crystal structure

Ngày tải lên: 21/12/2016, 10:50

... enzy-matic part, and (b) the variability of the angle of approach of the inhibitor relative to the catalytic cleft of the enzyme The latter factor may reflect not so much the geometry of the catalytic site ... chagasin–cathepsin L complex (Table 3) The dif-ference of 9 between these complexes may be corre-lated with the variation of the rate of binding and affinity for chagasin of these enzymes On the other ... despite the lack of overall sequence similarity The role of the proline residue appears to be to maintain the specific shape of the loop The aromatic residue, on the other hand, interacts with the

Ngày tải lên: 16/03/2014, 04:20

... that the v:w unit is linked to the single-stranded DNA-binding protein via the distal surface of v The base of the clamp-loader complex has an open C-shaped structure, and the shape of the v:w ... but, like the d and d¢ subunits of the clamp-loader, neither v nor w contain any of the functional elements required for nucleotide binding The topology of the w subunit resembles that of the bacterial ... to the c subunit and the b clamp As expected, the c subunit shows a high degree of conservation in the first two domains that make up the AAA+ATPase portion of the subunit The third domain of the

Ngày tải lên: 16/03/2014, 16:20

... num-bering of the amino acids in the RATEs) Interestingly, the crystal structure provides no clear indication of the reason for the decrease in catalytic activity as com-pared with RNase A Neither of the ... revealed that the linkers do not con-strain the ability of the RNase A entities to adopt the same orientation in the crystal as monomeric RNase A On the other hand, the decreased activity of the RATEs ... endocytosis [19] The stoichiometry of RI binding to RATEs and the role of the RI–RATE complex in the in vitro inacti-vation of RATEs have so far been obscure The crystal structures of three different

Ngày tải lên: 22/03/2014, 16:21

... shield the active site pocket by occupying the position corresponding to the lid-loop To better understand the significance of the absence of the lid-loop sequence and the presence of the extra ... in most other GGTs at the C-terminal region of the L-subunit In E coli GGT, upon autocatalytic cleavage of the peptide bond on the N-terminal side of Thr391, the C-terminal segment of the newly ... between the two enzymes can be seen in the manner of Fig 3 Comparison of the structures of GGTs from (A) B subtilis, (B) E coli and (C) H pylori The structure of each GGT is indicated in the upper

Ngày tải lên: 22/03/2014, 21:20

... allowing them to re-associate as domain-swapped di-mers Apart from differences in the course of the pro-tein backbone at the point of transition, in the swapped dimer the overall structure of the ... this stage The association of subdomains results in the formation of the closed b barrel burying the hydrophobic core At present there is no experi-mental evidence for the occurrence of the domain-swapped ... with the nucleobases pointing towards the protein surface The 5¢-to-3¢ polarity of the ligand follows that of most other nucleic acid com-plexes of OB-fold proteins, starting in the vicinity of

Ngày tải lên: 23/03/2014, 09:21

... [35].bFrom PROCHECK [38]. Trang 4the backbone and side chain of Asn34 and the side chain of Lys24 This crystal contact stabilizes the structure of the leading side of the foot loop The putative internal ... C-terminus, while Arg206 is on the top of the molecule, in the loop leaving helix 6 and it points in the opposite direction to the other basic residues (Fig 2B) The structure of the NLS is almost identical ... the side chain of Asn81 The intervening residues of the C-terminal extension interact with the neighboring monomer via hydrophobic contacts Comparison with the structure of the soluble form of

Ngày tải lên: 23/03/2014, 15:21

... 4) The structure of the C378G variant of SAP97PDZ2 was practically identical to that of C378S variant, except for the mutated residue, and because of its better resolution, only the structure of ... domain structures The solution structure of the second PDZ domain of PSD-95 has been determined by NMR [21], and, quite recently, coordinates of the PDZ2 domain of SAP102 crystallized in the absence ... SAP97PDZ2 C378S structure with the NMR structure of PSD-95PDZ2 and the crystal struc-ture of SAP102 The backbone strucstruc-tures are highly similar, except for some of the surface loops The loop bA-bB

Ngày tải lên: 30/03/2014, 10:20

... result, the structure of the complex is an average of the two half-sites Similarly, TL-3 was bound in the active site of the 12X FIV PR with its C2 axis of symmetry coincident with the crystallographic ... the flap region, and L97T, I98P, Q99V, P100N, and L101I in the "90s loop" region The 2-fold axis of the 12X FIV protease dimer is vertical in the plane of the figure; the C2 axis of the ... 63') The introduction of stabilizing contacts due to these mutations increases the overall stability of the closed conformation of the flaps (c) The stabilizing contacts formed as a result of the

Ngày tải lên: 13/08/2014, 09:20

... tools: can they indicate the information content of macromolecular crystal structures? Structure 6: 685-690 Dodson EJ, Kleywegt GJ, Wilson KS.1996 Report of a workshop on the use of statistical ... macromolecular structure determination Acta Crystallogr D 54: 905-921 Calderone V, Trabucco M, Vujicic A, Battistutta R, Giacometti GM, Andreucci F, et al 2003 Crystal structure of the PsbQ protein of ... Structure of recombinant human cyclophilin J, a novel member of the cyclophilin family Acta Crystallogr D 61: 316-321 Hubbard TJ, Blundell TL.1987 Comparison of solvent-inaccessible cores of

Ngày tải lên: 14/09/2015, 17:49

... selenomethionylated crystals were the same as that of the corresponding purified protein, indicating that the proteins are intact in the crystal and no part of them has been cleaved off during the crystallization ... and the other condition had 2.5% t-butanol The presence of these volatile precipitants was indispensable for crystal formation However, their volatile nature caused disintegration of the crystals ... HKL2000 program package The Matthew’s coefficient of the crystal indicated a solvent content of 57% and the asymmetric unit contained only one molecule The structure of the five LÆM mutant protein

Ngày tải lên: 14/09/2015, 17:50

... each other, the resulting amplitude is the sum of the individual amplitudes, if they are in phase with each other or the resulting amplitude is the difference of the individual amplitudes if they ... volume of the crystal sample arising when part of the crystal moves in or out of the incident beam Also, data are to be corrected for the thermal motion of atoms (which causes the fall-off of intensity ... -α-h-k-l So is the mirror image of in the real axis The real contribution and to the reflections of a Friedel pair are, like the structure factors themselves, reflections of each other in the real

Ngày tải lên: 14/09/2015, 17:52

... Mn incorporated into the crystal structure of BiFeO3 To further confirm the crystal symmetry of BSFMO samples, we refine the diffraction patterns by using the R3c model with the unit cell parameters ... good crystallinity of the polycrystalline ceramics All the diffraction peaks of samples are well in-dexed as per rhombohedrally distorted per-ovskite structure of BiFeO3 with a space group of the ... Mn-substitution samples The study of microstructure shows no obvious change of grain size and shape The magnetic properties of the as-prepared samples show the linear magnetic field de-pendence of magnetization,

Ngày tải lên: 17/08/2020, 20:37

... the overall structure is shown in Fig 3A The enzyme exists as a dimer of the abc heterotrimer There is a noncrystallographic twofold axis around the center of Fig 3A The structure of an abc heterotrimer ... irradiation [59] Therefore, we believe that the structure reported in this paper is also that of the glycerol dehydrataseÆcob(II)alamin complex The dihedral angle of the northern and southern least-squares ... 4C indicates the comparison of the position of the a-acetamide side chain of pyrrole ring A of the corrin ring in the glycerol dehydratase-bound cobalamin (Fig 4Ca) with those in the diol dehydratase-bound...

Ngày tải lên: 21/02/2014, 03:20

... al Crystal structure of NlpI A B C D Fig Solubility of NlpI constructs and the structure of mature NlpI (A) 10–20% gradient SDS ⁄ PAGE of NlpI expression products, showing the insolubility of the ... FEBS Crystal structure of NlpI natural TPRs in the PDB (13 TPR-containing coordinate sets) consist of nonglobular, extended arrays of helices [9,10,13–21] Second, the majority of these (11 structures) ... matches to the consensus the full-length HOP, they act to facilitate the assembly of multichaperone regulatory complexes The structural independence of these TPR domains, and the presence of independent...

Ngày tải lên: 07/03/2014, 16:20

... 2008 FEBS 2285 Structure of spliceosomal cyclophilin PPWD1 T L Davis et al Fig Structure of PPWD1 and comparison with other Cyp structures (A) The structure of the isomerase domain of PPWD1 is shown ... Acceleration of the cis–trans isomerization of the peptide results in the collapse of these resonances into a single set of peaks (D) Binding, but not isomerization, of the N-terminal peptide of PPWD1 ... that the homotypic interaction modeled in the crystal structure is supported by solution methods catalysis, explaining the capture of the trans conformer in the crystal [36] However, as the HIV...

Ngày tải lên: 23/03/2014, 07:20

... 20 A from the center of the funnel, they form the base of the b subunit (Fig 3) The last four residues in the C-terminal coil (residues 171–174) are deeply anchored inside the core of the conical ... catalytic domains of the a subunit In the heterohexamer, the Rieske domain interacts with the base of the adjacent b subunit and the catalytic domain of the adjacent a subunit Most of the ab interactions ... for each of the three a subunits LI, on the other hand, could only be partially modeled for one of the three a subunits, the high flexibility of the loop precluded modeling for the two other chains...

Ngày tải lên: 23/03/2014, 09:21

... Structure and thermal stability of T maritima 2852 In order to reveal possible determinants of the increased thermotolerance of TmIDH, we compared the structure of the dimeric form with the ... of the small domains showed an rmsd of ˚ 0.27 A for the Ca-atoms, whereas the rmsd between ˚ the large domains was 0.32 A The relative difference in the rotation of the large domain between the ... of the large domain in the active site This interaction mimics the phosphorylation of the equivalent serine in EcIDH which Structure and thermal stability of T maritima inhibits the binding of...

Ngày tải lên: 23/03/2014, 10:21

... diphosphate (ThDP) is the deprotonation of the C2 atom of the thiazolium ring (marked by an asterisk) The resulting ylid of ThDP (I) can attack the carbon atom of the carbonyl group of the substrate ... basis of the crystal structure of pyruvamide-activated ScPDC compared to that of ScPDC crystallized in the absence of any effectors [9], which is assumed to be the nonactivated state of the enzyme ... dimers The open and the closed side of the tetramer resulting from the special dimer arrangement are indicated Here, we describe the crystal structure of PDC from the yeast K lactis and the structural...

Ngày tải lên: 30/03/2014, 10:20

... on the basis of its X-ray structure [17] In this report, the stabilization mechanism of the hyperthermophilc b2 subunit will be discussed on the basis of the crystal structures, compared with the ... Crystal structure of tryptophan synthase b2 subunit alone (Eur J Biochem 271) 2625 structures of the a or b2 subunits alone as well as that of the complex The three-dimensional structure of the ... flexible region than the structure of the a and/or b subunits in the complex form reported Discussion Structure of Pf b2 and mutual activation Fig Crystal structure of b2 subunit alone of tryptophan...

Ngày tải lên: 30/03/2014, 14:20