signi cance of enzyme activity for substrate supply

... detection of the activity of microbial enzymes, including sialidases, in vaginal fluid has been demonstrated to be useful for the rapid diagnosis of BV (Wiggins et al., 2000, 2001) These enzymes ... indicating a high level of sialidase activity, while a yellow color was considered negative, indicating a normal level of sialidase activity The minimum detection limit of sialidase activity was 7.46 ... sialidase activity of the vaginal microflora: implications for the pathogenesis of preterm labour Int J STD AIDS 10(7), 442-447 Janulaitiene, M., Paliulyte, V., Grinceviciene, distribution of Gardnerella

Ngày tải lên: 29/05/2020, 10:57

... future flows of the substrate coupling II Substrate Noise Fundamentals i Parasitic Effect of Substrate [3] The parasitic material of substrate influences the behavior of a circuit design For example, ... Trang 1Analysis of Modeling Techniques for Substrate Noise CouplingBy Vincent Mui Abstract This paper presents an overview and comparison of several prevalent techniques for substrate noise coupling ... performance of both analog and digital signals in the integrated circuit In order to determine the amount of coupling between the sensitive nodes and noisy nodes, modeling techniques for substrate

Ngày tải lên: 20/10/2022, 08:13

... strategies of enzyme immobi-lization, each of which displayed different enzyme loading efficiency and activity More interestingly, the type of catalytic activity of the immobilized enzymes was ... =immobilized activity starting activity ×100% Efficiency (%) = observed activity immobilized activity×100% Activity recovery(%) =observed activity starting activity ×100% The immobilized activity ... enzyme loading is defined as: 2.3 Enzyme activity determination 2.3.1 Activity of immobilized and free enzymes To measure the observed activity of the immobilized enzymes, 20 mL 4 g/L DXT70K solution

Ngày tải lên: 01/01/2023, 12:27

... of a suspensionof a mid-logarithmic phase bacterial culture at an initial 600 nm UV absorbance of 0.001 in media required for good growth of the various bacterial strains was added to 10 lL of ... degreesÆcm2Ædmol)1by using mean residue masses of 110 R E S U L T S Identification of crucial residues for the antibacterial activity of pyrrhocoricin An Ala-scan was performed to identify key residues that ... insert Conformation of the DnaK fragments Although we assumed that the lack of pyrrhocoricin binding to some of the DnaK D-E helix sequences directly reflected the lack of pyrrhocoricin activity

Ngày tải lên: 08/03/2014, 10:20

... lower for wild-type enzyme than forD74N and E202Q, allowingflexibility of the gorge for entrance and bindingof substrates or inhibitors and exit of reaction products The carboxylic groups of E202 ... nondenaturatinggels showed that the enzyme preparations were mostly com-posed of dimeric forms (G2) of AChEs The wild-type enzyme preparation contained also traces of monomeric forms (G1) Enzyme concentrations ... respectively The specific activity of the wild-type enzyme was 2700 U mg)1 with ATC as substrate (1 U hydrolyses 1 lmol of ATC per minute at pH 7.5 and 25C) The specific activity of mutants were 350,

Ngày tải lên: 08/03/2014, 10:20

... of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato Yong-Qiang Gu* and Linda L Walling Department of Botany and Plant Sciences, University of California, ... coefficient was calculated for each peptide to determine the moles of peptide hydrolyzed Relative rates of hydrolysis were corrected for the percentage of enzyme in the active hexameric form, which was ... greater than 95% of its activity on the Leu-Leu peptide Rates of hydrolysis of other Xaa-Leu dipeptides were not impaired or were greater than 79% of the wild-type activity Fig 4 Model for the tomato

Ngày tải lên: 08/03/2014, 23:20

... overlapping fragments of GAS1 were amplified using two sets of primers For sGas1E161Q-H, a pairing of the forward (5¢-TGTTAGTAACTTGATTACCGGCGAAG-3¢), and a pairing of the forward primer FMGLN161 ... prior to division into two aliquots and the addition of the enzyme To test the effect of removal of N-linked chains on enzyme activity,
20 lg of native protein was treated with 10 mU Endo H in ... of an internal b(1,3)-glycosidic bond of a donor laminarioligo-saccharide followed by the transfer of the new reducing end to the nonreducing end of an acceptor molecule, with the formation of

Ngày tải lên: 16/03/2014, 18:20

... other substrates can be effec-tively utilized even by the open form of the enzyme The possibility of alternative mechanisms of cleavage for different substrates has important implications for the ... proxim-ity to the bound substrate, and allows optimal sub-strate positioning by the enzyme The evidence suggests that certain substrates require the formation of a closed form of the enzyme in order ... closed conformation Using the structure of the carboxypeptidase DcP, we have produced a model for the closed form of TOP with bound substrate The model allows for a more careful analysis of the

Ngày tải lên: 23/03/2014, 06:20

... is a much better substrate for the enzyme, with kcat⁄ KMof 3774 s)1Æm)1 versus 25 s)1Æm)1 for the H4 peptide, in agreement with histone H3 being the physiological substrate for P⁄ CAF [31] The ... at which half of the initial activity is lost in 5 min; T 50AcCo, temperature at which half of the initial activity is lost in 5 min in the presence of acetyl-CoA; t1⁄ 2, 48 C, activity half-life ... inactivation Catalytic properties and specificity of the P/CAF enzymes The kinetic parameters of the wild-type and mutant P⁄ CAF enzymes for acetylation of the histone H3 and H4 peptides were determined

Ngày tải lên: 23/03/2014, 06:20

... all enzymes of the clan SC and carrying residues important for the enzyme specificity, seems also to be very specific to each enzyme as it is clearly of a different size, D C Fig 1 Structures of enzymes ... characteristic of X-PDAP activity, as a broader com-parison involving all of the known structures of enzymes belonging to the clan SC has shown that most of these key residues are only present in X-PDAP enzymes ... (1QFS) [23], enzyme of the S9A subfamily; h_DPP-IV, the secreted part of human DPP-IV (1 N1M) [3], enzyme of the S9B subfamily and PepX from L lactis ssp cremoris (1LNS) [9], enzyme of the S15

Ngày tải lên: 23/03/2014, 13:20

... maximal activity of the enzyme at room temperature was achieved,i.e in the presence of 1.25M urea,the stability of the active site of the enzyme is significantly perturbed The optimal temperature for ... the conformation of the enzyme active site,we have determined the enzyme activity in the presence of up to 180 mMacrylamide At such relatively high concentrations of acrylamide the enzyme activity ... molecules of solvent and the quencher. Trang 8conformation of proteins because the enzyme activity of anumber of proteins is unaffected in the presence of acrylamide [29] Discussion Activation of NADH

Ngày tải lên: 23/03/2014, 15:21

... pock-ets engaged in complex formation of GABARAP and CRT(178–188) are also crucial for the GABARAP–K1 peptide complex (see below for details) Conformational changes upon complex formation The substantial ... of the alignment is given in the bottom panels of Fig 4 for the two hydrophobic pockets Binding of Trp183 (dark blue) in hp1 induces a slight shift of helix a2 (blue), similar to the effect of ... Binding of Phe185 does not have obvious consequences for the conformation of either hp1 or hp2 In contrast, Leu186 (dark blue) leads to a large displacement of helix a3 (blue) Binding of this

Ngày tải lên: 30/03/2014, 02:20

... affinity for substrates was unchanged, whereas catalytic activity was altered because the kcat value had decreased dramatically The structural study showed that the backbone conformation of the enzyme ... the bulky and rigid structure of histidine that can cause steric hindrances for the substrate, wherefore correct positioning and stabilization of the negative charge of E52 will be less than optimal ... changed, and the increase in the size of the side chain may create steric hindrance for the substrate, making the base moiety of the substrates bind in a nonoptimal conformation Another interesting feature...

Ngày tải lên: 19/02/2014, 02:20

... benefits of physical activity for health and well-being, as well as the current context of women’s health and participation in physical activity The general benefits of physical activity for health ... study of customary or habitual physical activity that found activity failed to significantly predict levels of morale or 38 BEYOND SHAPE AND SIZE: THE HEALTH BENEFITS OF PHYSICAL ACTIVITY FOR GIRLS ... equally so of women who are coping with many forms of diseases including breast cancer, heart disease and osteoporosis The activity can take many forms, from the joyous exertions of dragon boat...

Ngày tải lên: 05/03/2014, 13:20

... and effect of Ca2+ on the GTPase activity of recombinant TG2s GTPase activity was calculated as percentage of activity of the WT [99.1 ± 7.4 pmol GTP (minÆmg protein))1] in the absence of Ca2+ Data ... Transglutaminase activity was measured using mm CaCl2 and 0.4 lg of TG2 The reaction was started with 50 lL of enzyme mixture, and was performed at 37 °C for 30 Filter paper assay of transglutaminase activity ... Transglutaminase activity is shown as a percentage of the activity of wild-type TG2 One hundred per cent specific activity of wild-type TG2 was 8.4 DA405 (minÆmg))1 protein in the case of the microtiter...

Ngày tải lên: 16/03/2014, 03:20

... hydrolysis of substrate by HAP must function by an alternative mode of action [5] Resolution of a crystal structure for HAP would provide considerable insight into the unique functionality of this enzyme ... Standard dilutions of commercial BSA were used to generate a standard curve pH of maximal activity, enzyme kinetic assays and inhibition studies The pH of maximal activity for wild-type HAP and ... possible that its alteration to Lys is of functional signi cance Wild-type HAP, H34A and S37A exhibited a broad pH of maximal activity (5.0–8.5) (Fig 3) Activity in the basic pH range would also...

Ngày tải lên: 23/03/2014, 07:20

... redox state of the cysteinyl residues of apoFNR should be relevant for function and properties of the protein Therefore the redox state of the cysteinyl residues of the various forms of apoFNR – ‘aerobic’ ... conditions used here for FNR inactivation 4265 Disulfides of apoFNR ApoFNR is a product of the reaction of ([4Fe-4S]ÆFNR) with air The in vitro rate for the conversion of [4Fe-4S]ÆFNR to apoFNR (via [2Fe-2S]ÆFNR) ... on the redox state of the cysteinyl residues The rate of apoFNR formation is similar to that of [4Fe-4S] ⁄ [2Fe-2S] conversion, indicating that apoFNR is a signi cant product of FNR inactivation...

Ngày tải lên: 23/03/2014, 15:20

... used for activity assay or analysis by starch gel electrophoresis [2] After electrophoresis, gels were stained for ADH activity using 100 mM 2-buten-1-ol as a substrate Also, ADH activity of homogenates ... ADH1 shows substrate inhibition [31] and the contribution of ADH3 is still negligible because of its extremely low activity at pH 7.5 [7] One activity unit corresponds to the reduction of lmol NAD ... Detection of ADH1 and ADH4 in tissue homogenates of the gastrointestinal tract (A) Starch gel electrophoresis stained for activity using 100 mM 2-buten-1-ol as a substrate (B) Graphic representation of...

Ngày tải lên: 23/03/2014, 17:22

... year for which SSS is > 0.85 (number of trees needed) b Table II Summary of the regression coefficients expressing the effects of climatic factors on chronologies of Pinus pinea The signal of the ... of radial increment of P pinea was higher Climatic signi cance of IADFs in Pinus pinea 235 Figure Stabilized IADFs frequency in relation to calendar years The dot line represents the number of ... decreases the supply of carbohydrates for latewood formation and secondary thickening of cell walls This can explain the negative correlations between temperature in August(t) and latewood formation...

Ngày tải lên: 07/08/2014, 16:20

... structure of preferred substrates of TGase This information should be useful for predicting substrate reactivity and designing regulatory molecules that can interact with the active site of the enzyme ... effect on the reactivity of the peptide Assessment of the K5 sequence for reactivity and specificity in the peptide form To assess the reactivity and specificity of K5 in the peptide form, biotinylated ... function as substrates with similar levels of reactivity The reactivity and specificity of K5 for TGase was maintained even in peptide form Initially, we expected that the lysine residue of K5 would...

Ngày tải lên: 23/03/2014, 06:20