quantitative analysis and validation of method using hptlc

... of model system, aceclofenac and Soluplus, was estimated by two methods: by melting point depression of drug in presence of different polymer fractions and by Hildebrand and Scott solubility parameter ... miscibility of drug in polymer and the glass transition of the binary system (29) The present study is based on estimation ofχd-p using aceclofenac and Soluplus (a graft copolymer comprising of polyvinyl ... parameter of drug and polymer was small (~5 MPa1/2), which suggests mutual miscibility of the drug and polymer The value ofχd-pat 25°C was found to be 2.348 using Eq.5 Estimation ofχd-pUsing Melting...

Ngày tải lên: 11/05/2020, 11:57

... Metabolomics and Metabonomics Approach in the Study of Microcystin Toxicity 46 CHAPTER 2 –DEVELOPMENT OF METHODS OF ANALYSIS 48 2.0 Development of CE Methods for the Determination of Cyanotoxins ... Illustration of EOF inside a fused silica capillary 22 Figure 1 9: Illustration of the order of migration of ions in CE 22 Figure 1 10 Illustration of EOF profile 23 Figure 1 11: Illustration of sample ... electrophoresis (CE) methods for separation and quantification of cyanotoxins in water; and “metabolomic” and “metabonomic” approaches for investigation of the effect of exposure of a human cell line...

Ngày tải lên: 11/09/2015, 10:14

... Interpolation Method for Elastoplastic Analysis Int J Comput Methods 2015;12:1540013 [29] Liu GR, Zhao X, Dai KY, Zhong ZH, Li GY, Han X Static and free vibration analysis of laminated composite plates using ... Active vibration control of smart plates using directional actuation and sensing capability of piezoelectric composites Acta Mech 2013;224:1185–99 [64] Reddy KYL and XQP and GRL and JN A finite-element ... apply to the journal pertain Analysis and control of FGM plates integrated with piezoelectric sensors and actuators using cell-based smoothed discrete shear gap method (CS-DSG3) K Nguyen-Quang1,4,...

Ngày tải lên: 16/12/2017, 04:14

... for standard PCR amplifica-tion Electrophoresis verified all PCR products were specific with single bands in the gel (Fig S1) The melt-ing profiles of all amplified candidate reference genes using ... PEG6000 treatment Validation of reference genes In order to verify the expression stability of the se-lected reference genes by the software, expression of the TcMYB3 gene was quantified using either ... tissues, and 60S-18 and TUB-α for leaves under 4 °C treatment In this study, we used four methods, geNorm, Norm-Finder, BestKeeper, and RankAggreg, to evaluate the ex-pression stability of 20 candidate...

Ngày tải lên: 28/06/2021, 20:42

... modelling using OsiriX™ and Microsoft®Excel software The objectives of this study were 1- to provide a detailed description of the bone corridor analysis method and 2- to assess the reproducibility of ... different methods of angle deter-mination (automatically calculated vs manual) and between repeated measures were obtained using the Bland-Altman method and concordance correlation This method also ... description of a new method of AA OSIC analysis using OsiriX™ This method overcame the problem of subjective optimal im-plant placement definitions Three-dimensional simplifi-cation of bone corridors...

Ngày tải lên: 19/11/2022, 11:42

... at4500×gfor10minat4◦C,andfollowingcheckingofpHand specificgravity(SG),aliquoted(200␮L)into2mLmicrotubes Sam-pleswerefortifiedwith20␮Lofa 50ngmL−1 internalstandard mixsolutionandlefttostandfor15min,anda200␮Lvolumeof ... rateof0.15mLmin−1,whichtypicallygavepressuresof2.5×10-3 mbar.TheMSconditionswereoptimisedusingIntelliStartby infu-sionof1␮g mL−1 standard solutionsand 50%mobilephases A andBatflowratesof5␮Lmin−1and0.2mLmin−1,respectively ... matri-cesofchoice,faeceshavebeenproposedasanalternativematrix fortheanalysisofarylpropionamide-derivedcompoundsinbovine [15,16],canine[57]andrats[57,62].However,thereported screen-ingand/orconfirmatoryassaysaretypicallycapableofanalysisof eitherasingleSARMcompoundoralimitednumberofSARMsand...

Ngày tải lên: 25/12/2022, 00:41

... sensitivity of the ratio of output to input for variations in system character-istics and other parameters (c) Reduced effects of nonlinearties and distortion. (d) Increased bandwidth (bandwidth of a ... general areas of analysis and design of feedback control systems have influenced theformat of this book I would also like to thank and recognize all the undergraduate students inmechanical and electrical ... System analysis means the investigation, under specified condition, of the performance of a system whose mathematical model is known Analysis is investigation of the properties and performance of...

Ngày tải lên: 01/01/2014, 18:43

... multi-variate random processes. Understanding and knowledge of the random pressure field and its distribution is possible to interpret mechanisms of excitations, identification and response of aerodynamic ... applications of the Proper Transformations based on both cross spectral matrix and covariance matrix branches to analysis and identification of multi-variate random pressure fields. The random pressure ... VNU Journal of Science, Mathematics - Physics 24 (2008) 209-222 209 Analysis and identification of multi-variate random pressure fields using covariance and spectral proper transformations...

Ngày tải lên: 05/03/2014, 14:20

... for expression analysis, provides a satisfactory genetic marker system for mapping in rice using RNA hybridization and the RPP method of SFP analysis Methods Plant materials Seeds of rice (Oryza ... article Detection and validation of single feature polymorphisms using RNA expression data from a rice genome array Address: 1 Department of Botany and Plant Sciences, University of California, ... IR29 haplotype by RPP method are shown in squares (pink) and triangles (yellow), respectively Stars and vertical bars indicate the positions of the centromeres and the ends of chromosomes, respectively...

Ngày tải lên: 12/08/2014, 03:20

... sector in Zimbabwe using cost-effectiveness analysis and estimates of the burden of disease Address: 1 Institute of Public Health, Department of Health Services Research, University of Aarhus, Vennelyst ... provin-cial and central hospitals where hospital services of Trang 3increasing complexity are offered requiring more special-ised personnel and equipment The head office of the Ministry of Health and ... of office or department Further, a list of available equipment and fur-niture was developed and subsequently valued using mar-ket prices From these replacement costs of buildings, equipment and...

Ngày tải lên: 13/08/2014, 11:22

... [18]; and six profiles in nitrogen depletion after 8, 12 and 24 hours of treatment and in amino acid and ade- nine starvation after 1, 2 and 4 hours of treatment [19]. A complete description of ... 1, 1.5 and 2 hours of incubation [16]; six profiles in histidine starvation and various Gcn4 perturbations [17]; six profiles of chemostat growth in nitrogen limiting conditions with and without ... obtained using a uniform certainty level of 0.99 for all variables, but we obtained very similar results when using certainty levels of 0.95 and 0.9 (not shown). However, the robustness of our methods...

Ngày tải lên: 14/08/2014, 08:20

... UMG AND MGI METHODS··· 82 6.4.1 Comparison between the UMG/MGI and the RP Methods ··· 82 6.4.2 Comparison between the UMG/MGI and the SDCC Methods··· 82 6.4.3 Pros and Cons of the UMG and MGI Methods··· ... Trang 8Table of contents6.1 POPULAR COOLING METHODS··· 74 6.2 THE UMG AND MGI METHODS··· 76 6.2.1 The UMG Method ··· 77 6.2.2 The MGI Method··· 79 6.3 AUTO-DESIGN OF THE UMG AND MGI METHODS··· ... ELEMENT METHOD IN COOLING ANALYSIS AND DESIGN OF PLASTIC INJECTION MOULDS SUN YIFENG NATIONAL UNIVERSITY OF SINGAPORE 2003 Trang 2Founded 1905 FINITE ELEMENT METHOD IN COOLING ANALYSIS AND DESIGN...

Ngày tải lên: 17/09/2015, 17:20

... researchers and/or engineers for the development and validation of shiftingschedules and control systems.Transmission models consist of inertial elements, gear meshing, and elements that arecapable of ... electrification of trucks promotes significantadvantages in terms of energy and time, which translate into cost reduction A substantialnumber of electric trucks has been operating under tens of thousands of ... model of MSTsfor EVs, analysis of the topology changes of MSTs upon gear-shifting, and the range-prediction of EVs with MSTs In the following sections, the application of MSTs to EVs is explained...

Ngày tải lên: 10/01/2020, 10:39

... accuracy and repeatability Thus, the specified range of the method was 120 - 180 µg/mL * Determination of limit of determination and limit of quantification: The limit of detection and limit of quantification ... with the ratio of MeOH:H2O (65:35, v/v) was selected for quantitative analysis of NIF, because of good separation with a suitable retention time, compact and balanced peak 2 Method validation results ... Chromatogram of the NIF standard sample using Xbridge column Figure 3: Chromatogram of the NIF standard sample using Phenomenex LUNA column The results of asymmetry factor, the number of theoretical...

Ngày tải lên: 14/01/2020, 21:45

... 3further analysis of data because of their avail-ability and because of Zabbix features The correct choice of methods is crucial for any analysis The best way seems to use neu-ral networks and their ... it and do its analysis of his data MLP network is suitable for data classication and prediction and that was the right mathe-matical method to use We used Weka software for neural networks analysis ... focus and orientate in a huge amount of data, pre-processing is needed This part of the analysis is crucial It would not be possible to do complex analysis of all data in the monitoring system And...

Ngày tải lên: 11/02/2020, 18:24

... Non-random time splits represent one of the best validation methods when no truly external val-idation dataset is available and provide considerably more valid results than random splits of datasets; ... Systematic Overview of the Predictors used in the Analysis Table S3: Overview of all models in the backward selection procedure Methods S1: Detailed description and explanation of data analysis approach ... responsible clinicians and statisticians of the original RCT, and again by the statisticians of this secondary analysis Continuous predictor variables were kept continuous If age- and sex-specific...

Ngày tải lên: 20/02/2020, 21:59

... chromatography (HPTLC) with spectrometric methods It is because of the robustness and simplicity of use of HPTLC and the need for detection techniques that provide identification and determination of sample ... implementation, optimization, validation, automation, and qualitative and quantitative analysis; applications of HPTLC separation with special reference to phytochemical analysis, biomedical analysis, herbal ... static, off-line detection of zones using a great variety of complementary post-chromatographic universal and selective detection methods that are often applied sequentially, and storage of the...

Ngày tải lên: 06/10/2016, 09:26

... traditionally employed method (i.e construction of an EST library, storage and sequencing of each clone using Sanger sequencing technology), using high-throughput approaches allows several thousand ESTs to ... when regions of high DNA sequence homology tandem repeats and tandemly duplicated genes are present Gene sequences of interest were identified successfully using BAC sequencing Using lip transient ... filters were 458 nm/band-pass 465-510 nm for CFP, 488 nm/bandpass 500-530 nm for GFP, 514 nm/ bandpass 525-555 nm for YFP, 561 nm/bandpass 575-630 nm for RFP and mCherry, and 488 nm/band-pass 650-710...

Ngày tải lên: 11/08/2014, 11:21

... Materials and methods Plasmid construction In general, PCR amplifications were performed with VentDNA-polymerase (New England Biolabs) The entire sequences of the dfp and coaB coding regions of the ... described [5] Purification and characterization of Dfp and CoaB proteins Growth of strains E coli M15 (pREP4, pQE8/pQE12) cells were grown in the presence of ampicillin (100 lgÆmL)1) and kanamycin (25 ... and 450 nm Molecular mass information was obtained as described [6], determining the elution volumes of standard proteins and the void volume of the column The Superdex 200 PC 3.2/30 column and...

Ngày tải lên: 19/02/2014, 12:20

... computer modelling of the MUC1 SEA domain (Fig 3) The model has the same a ⁄ b sandwich fold as the template SEA domain of murine MUC16 [12] and consists of three a-helices and six b-strands This model ... Ribbon representation of the modelled SEA domain of human MUC1 The model consists of three a-helices and six b-strands forming an a ⁄ b sandwich fold The FRPG ⁄ SVVV residues of the MUC1 cleavage ... MUC3SEA and MUC1FDTR ⁄ MUC12SEA glycoproteins showed a major M2-reactive and CT-2 reactive noncleaved glycoform of about 105–120 kDa (Fig 4A,C, MUC3SEA and MUC12SEA) and a minor population of a...

Ngày tải lên: 19/02/2014, 18:20