production of microbial b mannanases

... measured the Ab level secreted from wt fibroblasts overexpressing APP [23] As shown in Fig 5A, B, the overexpression of < /b> Synoviolin enhanced the production < /b> of < /b> Ab40 and Ab42 by about twofold, whereas ... synoviolin-null fibroblasts (A) Cell surface proteins of < /b> synoviolin-null and wt fibroblasts were biotinylated as described in Materials and methods The lysates of < /b> surface-biotinylated cells were then incubated ... internal markers ) ⁄ ), synoviolin-null fibroblasts; + ⁄ +, wt fibroblasts (B) NCT in the lysates from wt fibroblasts (left panel) and synoviolin-null fibroblasts (right panel), retrovirally expressing...

Ngày tải lên: 23/03/2014, 04:20

... strains bred by gene-, protein-, and metabolic engineering or by combinations of < /b> these types of < /b> engineering (hydroxy-l-proline) In this paper some representative examples of < /b> microbial < /b> production < /b> of < /b> ... acid-producing bacteria were reported after the first report on Corynebacterium glutamicum, including Brevibacterium flavum, Brevibacterium lactofermentum, Brevibacterium thiogenitalis, and Microbacterium ... favorable strain for the production < /b> of < /b> l-alanine since it showed the highest activity with aspartate b- decarboxylase At first, the production < /b> of < /b> l-alanine by immobilized cells were accomplished by...

Ngày tải lên: 26/10/2013, 02:20

... site of < /b> ChiB has dened subsites from )3 to +3 [16,20], and the substrate-binding cleft is tunnel-like, permitting tight interaction with the polymeric substrate The aglyconside of < /b> the substrate-binding ... Size-distribution of < /b> oligomers after ChiB degradation (FA ẳ 0.65) Chromatograms showing the size-distribution of < /b> oligomers obtained after hydrolysis of < /b> chitosan (FA ẳ 0.65) with ChiB to degrees of < /b> scission ... Peaks are labelled with DP-values or with the sequence of < /b> the oligomer 542 FEBS Journal 272 (2005) 538549 ê 2005 FEBS A Sứrbotten et al Degradation of < /b> chitosans with ChiB Table Composition of < /b> isolated...

Ngày tải lên: 07/03/2014, 16:20

... progeny (VA6-4) of < /b> VA6 All three plants show bands expressed from cryIA (b) Bt gene Besides the expected 65 kDa, two additional bands between 46 and 30 kDa were also observed The amount of < /b> Bt protein ... M.E Fromm, I.K Vasil, Rapid production < /b> of < /b> transgenic wheat plants by direct bombardment of < /b> cultured immature embryos, Bio/ Technology 11 (1993) 1553 – 1558 D Becker, R Brettschneider, H Lorz, Fertile ... study Southern blot analysis (Fig 2) showed both transformants had the 1.8 kb coding sequence of < /b> the cryIA (b) gene (plasmid pCIBBt1; Fig 1) Multiple bands in VA6 indicated that recombination and...

Ngày tải lên: 23/03/2014, 22:20

... NF- B is markedly increased in the RA synovium [12,13] IL-17 has been shown to instigate a rapid degradation of < /b> inhibitor of < /b> B in RA synovial fibroblasts [4], indicating that activation of < /b> NF- B ... stimulation of < /b> the extracts with 10 ng/ml of < /b> IL-17 or IL-1β were tested by gel mobility shift assay of < /b> radiolabeled oligonucleotide probes representing the NF- B sites in the promoter of < /b> (a) IL-6 and (b) ... with IL-1β FLS, fibroblast-like synoviocytes; RA, rheumatoid arthritis been reported from human colonic myofibroblasts [20], where administration of < /b> SB203580, a chemical inhibitor of < /b> p38, significantly...

Ngày tải lên: 09/08/2014, 01:23

... [30] As I B is a major inhibitory component of < /b> the NF B pathway, increased expression of < /b> I B blocks NF B nuclear translocation and DNA binding induced by LPS We then examined whether I B overexpression ... construction of < /b> adenovirus vectors Mol Biotechnol 1995, 3:207-220 Bondeson J, Browne KA, Brennan FM, Foxwell BM, Feldmann M: Selective regulation of < /b> cytokine induction by adenoviral gene transfer of < /b> I B ... overexpression inhibits monocyte chemokine production < /b> induced by Ttcr cells We found that AdI B inhibits the production < /b> of < /b> CC chemokines induced by contact with Ttcr cells but has no effect on...

Ngày tải lên: 09/08/2014, 08:23

... containing MBP From the mobility of < /b> the MBPK activity band during SDS-PAGE, the apparent molecular mass of < /b> Page of < /b> 17 (page number not for citation purposes) BMC Plant Biology 2007, 7:61 http://www.biomedcentral.com/1471-2229/7/61 ... Staurosporine, a potent inhibitor of < /b> serine-threonine kinases, SB 203580, an inhibitor of < /b> P38 class of < /b> MAP kinase, PD 98059, an inhibitor ERKK class of < /b> MAPKK and SB 600125, an inhibitor of < /b> Janus kinases ... UV -B- induced MBPK activity whereas the UV-Binduced CDPK activity could not be completely inhibited by staurosporine and was not inhibited by SB 203580, PD 98059 and SB 600125 pretreatments of...

Ngày tải lên: 12/08/2014, 05:20

... anti-TNF-α mAb (PBL Biomedical Laboratories), and allophycocyanin (APC)-labeled anti-BDCA4 mAb (Miltenyi Biotec, Bergisch Gladbach, Germany) Dead cells were excluded on the basis of < /b> side- and ... inhibits the production < /b> of < /b> these cytokines by myeloid DCs Although TNF-α and IL-12 production < /b> were impaired by 10-7 M of < /b> BAY11, IFN-α production < /b> was not significantly inhibited by doses of < /b> up ... loxoribine) were markedly inhibited in a dose-dependent manner (Figure 1B) By contrast, TNF-α production < /b> by PBMCs in response to these TLR ligands was only modestly inhibited by the 10-6 M of < /b> BAY11...

Ngày tải lên: 12/08/2014, 12:20

... PRODUCTION < /b> OF < /b> LACTIC ACID BY MICROBIAL < /b> FERMENTATION OF < /b> HEMICELLULOSE SUGARS PUAH SZE MIN (B. Sc NUS) A THESIS SUBMITED FOR THE DEGREE OF < /b> MASTERS OF < /b> SCIENCE DEPARTMENT OF < /b> CHEMISTRY ... them that I dedicate this work I TABLE OF < /b> CONTENTS ACKNOWLEDGEMENTS I TABLE OF < /b> CONTENTS II LIST OF < /b> TABLES V LIST OF < /b> FIGURES VI LIST OF < /b> SCHEME IX LIST OF < /b> ABBREVIATIONS X SUMMARY XII CHAPTER 1 ... parameters 58 BIBILIOGRAPHY AND NOTES 59 IV LIST OF < /b> TABLES Table Microorganisms used in the recent work of < /b> biotechnological production < /b> of < /b> lactic acid Table Recent investigation of < /b> xylose utilizing...

Ngày tải lên: 16/10/2015, 11:57

... Southeast Asia Before HBV vaccine was integrated into the routine immunization program, the proportion of < /b> babies that become HBV carriers is about 10-30% for mothers who are HBsAg-positive but HBeAg-negative ... the global disease burden of < /b> HB is substantial Epidemiology The prevalence of < /b> chronic HBV infection varies greatly in different part of < /b> the world (Figure 1) The prevalence of < /b> chronic HBV infection ... characteristics, such as HBV DNA or ALT levels [41] Occult Hepatitis B Occult hepatitis B is defined by the presence of < /b> HBV DNA in serum or liver in the absence of < /b> HBsAg [42,43] Serum HBV level is usually...

Ngày tải lên: 02/11/2012, 11:12

... establishes, the serology markers like HBeAg, HBeAb and HBsAb can be positive or negative except HBsAg and HBcAb (IgG form) remain positive HBeAg positive chronic hepatitis B Age at the time of < /b> ... infection, HBsAg becomes detectable after to 10 weeks of < /b> incubation period, followed by antibodies against the core antigen (HBcAb) in IgM form during early period [2] Viremia is well established by the ... blood and liver begins to drop With clearance of < /b> the infection, HBsAg and HBeAg disappear, and free HBsAb become detectable Presence of < /b> HBsAg, HBeAg, and high DNA level for greater than months...

Ngày tải lên: 02/11/2012, 11:17

... species can only be obtained by use of < /b> the FISH technique Microbial < /b> diversity of < /b> ammonia-oxidizing bacteria The phylogenetic microbial < /b> diversity of < /b> two types of < /b> nitrifying biofilms, a domestic ... Watanabe 1996 Spatial microbial < /b> distributions of < /b> nitrifiers and heterotrophs in mixed population biofilms Biotechnol Bioeng 50:24-35 Okabe, S., H Satoh, and Y Watanabe 1999a In situ analysis of < /b> ... of < /b> a vertical biofilm thin section with TRITC-labeled Nso190 probe specific for NH4+-oxidizing bacteria of < /b> the beta subclass of < /b> the Proteobacteria (red stain clusters) and FITC-labeled Ntspa454...

Ngày tải lên: 05/09/2013, 08:40

... spectrum of < /b> peak b and that of < /b> the standard compound (data not shown), peak b was identified as a 3M4NP derivative The production < /b> of < /b> 3M4NP derivative would be due to contamination of < /b> a derivatizing ... 2-chloro-5-methyl-4-nitrophenol (2C5M4NP) was obtained by custom order from Chemical Soft R&D Inc (Kyoto, Japan) 20 mg of < /b> each of < /b> the chemicals is dissolved into L of < /b> distilled water and mg-Cl/mg-C of < /b> sodium hypochlorite ... shown in Fig 1 (B) was obtained from peak c (RT = 19.3 min) Because m/z 210 ion was observed in the mass spectrum, production < /b> of < /b> trichloro-3-methylphenol (TC3MP) in the sample of < /b> chlorinated fenitrothion...

Ngày tải lên: 05/09/2013, 10:15

... molecule are bound by weak hydrogen bonds The strong absorption centered at 710.55 cm-1 is the typical Ti-O-Ti vibration [21-23] The characteristic peak of < /b> – SO3- group of < /b> Nafion of < /b> 1240 and 1132 ... electrolyser 2.5 Performance of < /b> the membrane electrode assembly (MEA) The performance of < /b> prepared MEA is evaluated using in-house fabricated single cell assembly A DC voltage of < /b> 1.8 to 2.6V is applied ... one of < /b> the important characterization of < /b> PEM is Ion exchange capacity (IEC) It can be seen that Composite membrane exhibited relatively better IEC value (0.96 meq g-1) in comparison to that of...

Ngày tải lên: 05/09/2013, 16:11

... dehydrogenase, BDH: β -diketone hydrolase 295 296 12 Modeling and Simulation of < /b> Microbial < /b> Depolymerization Processes of < /b> Xenobiotic Polymers of < /b> carbon–carbon chain between two carbonyl groups/a carbonyl ... 12.4 HPLC profiles of < /b> PEG before and after the cultivation of < /b> the microbial < /b> consortium E-1 [11, 12] 289 290 12 Modeling and Simulation of < /b> Microbial < /b> Depolymerization Processes of < /b> Xenobiotic Polymers ... Kawai, F (2002) Microbial < /b> degradation of < /b> 10 11 polyethers Appl Microbiol Biotechnol., 58, 30–38 Kawai, F (1995) Breakdown of < /b> plastics and polymers by microorganism Adv Biochem Eng Biotechnol., 52,...

Ngày tải lên: 25/10/2013, 08:20

... under their belts, Sage says the overall airport fiber and copper-backbone project is about onequarter to one-third completed “Before we’re finished, we’ll be running another km of < /b> duct bank so when ... Despite the emphasis on fiber, the project did involve some copper Because some of < /b> the older control systems could not be made compatible with fiber, they ordered 2.5 km of < /b> 19-gauge, 100-pair copper ... network between buildings The high priority of < /b> these critical issues can be seen throughout the system, in what Sage describes as “an equipment-incabinet philosophy Many areas are accessible to...

Ngày tải lên: 24/01/2014, 10:20

... small number of < /b> BPPs have been isolated and studied, including Shewanella oneidensis MR-1 PhyS [6], Bacillus subtilis PhyC [7], Bacillus sp DS11 Phy [8], B subtilis 168 168PhyA [9], Bacillus ... of < /b> the NCBI database, e.g those of < /b> Shewanella sp., Pseudomonas sp and Idiomarina sp [5], suggesting the prevalence of < /b> dual-domain BPPs in c-Proteobacteria Notably, PhyH is the first Bacillus dual-domain ... Application of < /b> microbial < /b> phytase in fish feed Enzyme Microb Tech 40, 497–507 FEBS Journal 278 (2011) 3032–3040 ª 2011 The Authors Journal compilation ª 2011 FEBS 3039 Catalysis of < /b> a dual-domain b- propeller...

Ngày tải lên: 14/02/2014, 15:20

... altered metabolism of < /b> Ab, in particular aggregation-prone Ab species like Ab42, initiates AD pathogenesis Oligomeric assemblies of < /b> Ab trigger aggregation of < /b> tau and the formation of < /b> NFTs, but also ... plaques profound CAA some neuron loss Neuritic plaques and CAA Neuropathology Promoter Transgene Model PDGF Contact Ratio brain Ab ⁄ CSF Ab CSF Ab Ratio brain Ab ⁄ plasma Ab Plasma Ab Brain Ab (pmolÆg)1) ... in the absence of < /b> hemorrhage and an abundance of < /b> parenchymal Ab deposits lacking amyloid cores in postmortem brain [82] The Arctic mutation promotes Ab protofibril and fibril formation, but also...

Ngày tải lên: 16/02/2014, 09:20