pipeline read alignment and variant calling

... ChIP and analyzed the data TAE carried out ChIP-chip of H3K9 and H3K27 methylation SS helped to draft the manuscript KI and JDL designed experiments and wrote the manuscript All authors read and ... http://genomebiology.com/2010/11/12/R120 the aligned reads computationally to 200 bp (H3Kme3) or 300 bp (LEM-2, control IgG and input DNA) The numbers of the aligned reads and the coverage of the extended reads on the genome ... Durbin R: Mapping short DNA sequencing reads and calling variants using mapping quality scores Genome Res 2008, 18:1851-1858 60 Bolstad BM, Irizarry RA, Astrand M, Speed TP: A comparison of normalization...

Ngày tải lên: 09/08/2014, 22:23

... target and novel visual stimuli in children of alcoholics Alcohol 1998; 15:119-36 92 Porjesz B And Begleiter H Human brain electrophysiology and alcoholism In: Tarter R and Thiel DV, Eds Alcohol and ... both active and passive P300 latency and age; in this same study, levels of active latency were associated with concentration ability, and passive latency with verbal proficiency and recall [72] ... 11:141-151 67 Polich J, Hoffman D P300 and handedness Psychophysiology 1988; 35:497-507 68 Hopper KD, Patel S, Cann TS, et al The relationship of age, gender, handedness, and sidedness to the size of the...

Ngày tải lên: 02/11/2012, 11:08

... The Netherlands Natural disasters have increased in frequency and severity during the last few decades, causing considerable economic damage and loss of life A combination of climate and socio-economic ... resulting in the spread of diseases in affected areas, and have major adverse economic consequences caused by damage to property, both direct and indirect, such as business interruption and productivity ... Climate change and the frequency and severity of natural disasters In addition to socio-economic developments, climate change may increase the intensity and severity of weather extremes and contribute...

Ngày tải lên: 07/10/2012, 15:57

... kind and peaceful strong and brave attractive and handsome thoughtful and wise talented and artistic popular and well-liked funny and amusing rich and influential truthful and sincere strong and ... Getting Ready to Read activity, consisting of questions for discussion, predictions, or vocabulary to help students understand the reading; the actual reading passage; and an After You Read activity ... discussion and more time being allowed for writing assignments or tape journal entries Threads: Threads are placed in the margins of the text and appear in two forms—quotes and facts Factual threads...

Ngày tải lên: 14/12/2013, 15:07

Ngày tải lên: 18/12/2013, 21:17

Ngày tải lên: 18/12/2013, 21:17

... Bandwidth – Two priority queues per policy – The set cos command, set cos-inner command, set cos cos-inner command, and set cos-inner cos command – WRED aggregate – Queue-limit SUMMARY STEPS enable ... Plus (ES+) and Ethernet Services Plus T (ES+T) Line Card Configuration Guide 4-16 OL-16147-04 Chapter Configuring Layer and Layer Features Flexible QinQ Mapping and Service Awareness – Bandwidth ... Mapping and Service Awareness Flexible QinQ Mapping and Service Awareness allows service providers to offer triple-play services, residential Internet access from a DSLAM, and business Layer and...

Ngày tải lên: 25/01/2014, 11:20

... infection and multiplication of the HPAI virus were detected in the transfected cells [13] MSPL and TMPRSS13 are expressed in a variety of tissues, and predominantly in lung, placenta, pancreas and ... conditions and stained with Coomassie Brilliant Blue The incubation generated two main bands of  60 and 32 kDa (Fig 4A) The sizes corresponded 4892 Fig Proteolytic conversion of pro-HGF by TMPRSS13 and ... TMPRSS13 mRNA was detected in MKN45 and MKN74 cells, but not in A549, LoVo and HepG2 cells On the other hand, HAI-1 mRNA was detected in LoVo, MKN45, MKN74 and HepG2 cells (Fig 6) These results...

Ngày tải lên: 15/02/2014, 01:20

... to CeMT-1 and CeMT-2 if presented with Zn : Cd ratios as encountered by C elegans Using a Cd : Zn ratio of 33 : [21 nm Cd(II) and 0.7 lm Zn(II)] and 0.1 lm of CeMT-1 and CeMT-2 each, and the stability ... metal affinities and their comparison, it was very important to obtain clearly defined homo-metallic species, and the data compiled for Zn6-CeMT-2, Cd6-CeMT-2 and Zn7-CeMT-1 in Fig and Table show ... Table Zinc and cadmium binding affinities for MTs in Caenorhabditis elegans and other species Log K binding constants for zinc and cadmium metalloforms of C elegans MTs were determined and compared...

Ngày tải lên: 16/02/2014, 15:20

... concentration after stimulation with EGF and PMA together on the one hand, and the sum of the ERK-PP concentrations after stimulation with EGF and PMA separately on the other hand, was not constant, but increased ... NaCl/Pi and washed Fig Biphasic ERK-PP time profile induced by EGF or PMA alone and synergistic ERK phosphorylation induced by EGF and PMA together Cells were serum-starved for three days and subsequently ... Raf1 phosphorylates MAPK/ERK kinase (MEK) and 2, which in turn phosphorylate ERK1 and ERK2 Phosphorylated ERK (ERK-PP) has several different cytoplasmic and nuclear targets Transcription factors...

Ngày tải lên: 19/02/2014, 16:20

... gelsolin S2 sequences (and homologue S2 equivalents) within residues 197–226 (including the long helix of the domain) and within residues 161 – 172 (including the A strand and the AB loop in S2) ... fragments [19] of 31 and 16 kDa (1 –225 and 226–375 sequence, respectively) As shown in Fig 5, digestion of the cross-linked actin peptide complex reveals two faint bands at 33 kDa and 46 kDa which ... 338–348, and the other to two helices and one turn located in the 356–375 sequence The corresponding peptides (339– 349, 347–365, 356–375 and 360–372) were coated to plastic We observed (Fig 8A and...

Ngày tải lên: 22/02/2014, 07:20

... underlined An EF-hand consensus sequence is placed between the aligned EF-hand I and EF-hand II sequences [3] The helices are shown as boxes with conserved hydrophobic (h) and other amino acids ... calcium-binding ligands are designated by their coordinates: x, y, z, -y, -x and -z [60] Residues discussed in the text are given in bold (G34, K60 and G81 of CR I –II and G29, G66, R68, G71 and G74 of ... occurred and a lower band, most probably GST, was observed We observed that CR, but not GST (also the lower bands of lanes and 3), binds 45 Ca under the conditions used GST–CR(1–60) and GST–...

Ngày tải lên: 22/02/2014, 07:20

... washed twice and resuspended in 50 lL of M9, and then transferred into the chamber already containing 450 lL of M9 buffer, and respiration was measured at 20 °C for at least 10 All washes and measurements ... the body wall muscles (Fig 2C) and in the rectal gland cells in larvae and adults (Fig 2D) The presence of a peroxisomal targeting signal in its C-terminus (Fig 1) and the punctate localization ... these data indicate that GSTK-1 and GSTK-2 have a peroxisomal and mitochondrial localization, respectively Impairment of oxygen consumption and lipid content in gstk-1 and gstk-2 double-knockdown...

Ngày tải lên: 16/03/2014, 03:20

... Sampling and analyses of foodstuffs from animal origin Robin S Readnour, Thomas J Burnett, Douglas E Kiehl and Lisa D Spurlock-Brouwer Introduction Sample collection and storage Sampling and homogenization ... Isoxaflutole Robert J Seymour, Craig A Smitley and Sabrina X Zhao 509 Introduction Outline of method Reagents and standards Equipment and supplies Sampling and preparation Extraction procedure Manual ... handling and shipment QA and field data requirements Data reporting Data presentation and interpretation Case studies overview Case study with Diazinon 50W Fortress-5G case study Conclusions and...

Ngày tải lên: 17/03/2014, 02:20

... casement and calling softly to her, to open it and speak to him, or calling her by name, with his extraordinary foreign accent And he thought he was tapping louder and louder, upon the glass and upon ... iron trestles, and wooden stools of well-worn brown oak, and tables painted a vivid green, and primitive lithographs of Saint Benedict and Santa Scholastica and the Addolorata And there were ... of maize and wheat, waiting to be picked over before being ground in the primeval household mill And there were hams and sides of bacon, and red peppers, and bundles of dried herbs, and great...

Ngày tải lên: 17/03/2014, 13:20

... lane, two bands corresponding to GST-FL (upper band) and GST (lower band); Nt lane, GST-Nt peptide (upper band) and GST (lower band); Ct lane, only one band (GST-Ct peptide) (B) SDS ⁄ PAGE analysis ... (A) and FL peptide (B) The inhibitor concentrations were 0.125 mM (s) and 0.0625 mM (d) in each case Water (h) was used as a control Fig Sequence alignment of the Nt and Ct peptides of taro and ... amplification of the FL peptide; F1 and R2 was for the Nt peptide, and F2 and R1 was for the Ct peptide The PCR products were digested with BamHI and EcoRI, and ligated to pGEX-2TK vector (Amersham...

Ngày tải lên: 23/03/2014, 06:20

... both Spd and Spm substrates In particular, HvPAO1 enzymatic activity showed an optimum at pH 7.0 for Spm and Spd oxidation and Km values of 0.89 · 10)5 m and of 0.50 · 10)5 m for Spd and Spm substrates, ... expressed in roots, leaves and ears of barley, and in roots and leaves of maize with the aid of a biolistic delivery system In this study, we present evidence that barley HvPAO1 and HvPAO2 genes represent ... HvPAO1 and HvPAO2 mRNAs in different barley tissues The transcription level of HvPAO1 and HvPAO2 mRNAs has been examined in different stem internodes and whole ear at 30-days postfertilization and...

Ngày tải lên: 23/03/2014, 10:21

... Textile, Clothing and Footwear Mechanic Metal Fitters and Machinists nec Engraver Gunsmith Precision Instrument Maker and Repairer Saw Maker and Repairer Watch and Clock Maker and Repairer Engineering ... Schedule and Australian Government Department of Immigration and Citizenship OCCUPATION Judicial and Other Legal Professionals nec Careers Counsellor Drug and Alcohol Counsellor Family and Marriage ... 2012– Schedule and Australian Government Department of Immigration and Citizenship OCCUPATION Wall and Floor Tiler Business Machine Mechanic Communications Operator Cabler (Data and Telecommunications)...

Ngày tải lên: 29/03/2014, 23:20

... (compare lanes and 1) and the same holds true for the GST–SAFB1CDRE protein (lanes and 4), whereas SAFB2 barely binds to SRPK1a (lane 6) but almost as tightly as SAFB1 to SRPK1 (compare lanes and 1) ... (lane 6) As shown in Fig 6B, both GFP–SAFB1 and GFP–SAFB2 proteins bind to both kinases (left and right panels, lanes 2, and 5, 6), unlike GFP (lanes and 4) which does not bind by itself However, ... BamHI site and reverse: 5¢-TTGAATTCTCAGTAGCGGCGAGT GAA-3¢, containing the underlined EcoRI site The PCR fragment was digested with BamHI and EcoRI, repurified and subcloned into the BamHI and EcoRI...

Ngày tải lên: 30/03/2014, 01:20