mutm single c2c2 zinc finger dna interaction

... analysis of CCCH zinc finger family in Arabidopsis and rice BMC Genomics 2008, 9:44 Blackshear PJ, Phillips RS, Lai WS: Tandem CCCH zinc finger proteins in mRNA binding In Zinc Finger Proteins: ... Carballo E, Thorn JM, Kennington EA, Blackshear PJ: Interactions of CCCH zinc finger proteins with mRNA binding of tristetraprolin-related zinc finger proteins to Au-rich elements and destabilization ... protein-binding proteins [4-7] Recently, a new type of Arabidopsis zinc-finger proteins, which differs from the previously identified plant zinc-finger TFs by regulating gene expression via directly binding

Ngày tải lên: 01/11/2022, 09:06

... Trang 1R E S E A R C H A R T I C L E Open AccessTargeted disruption of Tbc1d20 with zinc-finger nucleases causes cataracts and testicular abnormalities in mice Anna Kyunglim Park1, Ryan ... the phenotypic consequences in mice caused by the disruption of the Tbc1d20 gene Results: The zinc finger nuclease (ZFN) mediated genomic editing generated a Tbc1d20 c.[418_426del] deletion encoding ... establish TBC1D20-mediated molecular and cellular functions Keywords: TBC1D20, Loss-of-function, Zinc-finger nuclease, Blind-sterile, Spermatogenesis, Warburg Micro Syndrome * Correspondence: dsidjani@mcw.edu

Ngày tải lên: 27/03/2023, 04:25

... và sàng lọc trên Pfam (El-Gebali et al., 2019) Trình tự nucleotide, bao gồm gDNA (genome DNA seq), CDS (coding DNA seq) và trình tự protein của tiểu phần YA lần lượt được thu thập cho các phân ... dạng di truyền nhóm Bacillus subtilis bằng phương pháp giải trình tự đoạn protein ngón tay kẽm (Zinc finger protein) và kỹ thuật PCR dùng mồi thiết kế trên các chuỗi lặp (REP-PCR) 7 Nguyễn Thị Kim

Ngày tải lên: 15/03/2024, 10:42

... inactivate DNA repair processes by t he oxidation of zinc finger structures in DNA repair proteins. As these observations are derived from subcellular systems, experiments on interactions with DNA repair ... proteins with zinc finger domains [19]. It is assumed that many of these proteins are regulated by the oxida tion of zinc-binding cysteine residues, which leads to a loss of DNA binding, zinc release ... release zinc from MT [13–16]. We demonstrate that the reducible selenium compounds included in this study (a) inhibit Fpg activity, (b) modulate XPA–DNA interactions, and (c) release zinc from

Ngày tải lên: 30/03/2014, 15:20

... CornishỜBowden relation [25]: dơDNAP kcatơIN0đơDNA0 ơDNAPỡ Kmợ ơIN0ợ đơDNA0 ơDNAPỡ đ14ỡ Eqn (14) is valid in two cases: either [IN]0 [DNA]0 (single turnover) or [DNA]0 [IN]0 (multiple turn-A ... viral DNA (substrate DNA), a reaction termed 3¢-end processing In the second step, DNA strand transfer, a pair of processed DNA ends of the same viral DNA is inserted into the host cellular DNA ... of excess DNA substrate, indicating that IN has a limited turnover for DNA cleavage Therefore, IN works principally in a single-turnover mode and is intrinsically very slow (single-single-turnover

Ngày tải lên: 16/03/2014, 13:20

... analytes [9–11], in DNA nanotechnology [12,13], and in DNA computation [14–16] T4 DNA ligase, the prototype of ATP-dependent DNA ligases [17–19], is the most commonly used DNA ligase One factor ... able to join the ends of single-stranded DNA in the absence of any duplex DNA structure at the ligation site Such nontemplated ligation of DNA oligomers catalyzed by T4 DNA ligase occurs with a ... on ssDNA substrates [29,30],this property of T4 DNA ligase has, to our knowledge, not been reported Unlike T4 DNA ligase, bacterial DNA ligases that we tested did not have any detect-able ssDNA

Ngày tải lên: 23/03/2014, 11:20

... process 3¢-end-modified DNA strand breaks are required for efficient recruitment of X-ray cross-complementing protein 1–DNA ligase IIIa heterodimer to the sites of DNA repair DNA single-strand breaks ... binding is not dependent on XRCC1–DNA ligase IIIa heterodimer Interaction between PNK and XRCC1 is required for effective XRCC1–DNA ligase IIIa recruitment to the site of DNA damage PNK interacts with ... 5753–5763 ª 2005 FEBS J L Parsons et al Mammalian DNA single-strand break repair A B C Fig Interaction between PNK and XRCC1 is required for efficient XRCC1–DNA ligase IIIa binding to a 3¢-end phosphate-containing

Ngày tải lên: 23/03/2014, 11:20

... HAKN1–DNA interaction Based on the analysis of the binding of wild-type and mutant HAKN1 homeodomains to different DNA tar- get sites, we propose a model for the interaction of HAKN1 with DNA. ... contacts with this region of DNA. The interaction of mutants at position 50, and par- ticularly of I50K–HAKN1 with DNA, provides a framework to build a model of HAKN1–DNA inter- actions, taking ... DNA–protein contacts proposed by this model. Discussion In this study, we investigated the interaction of the homeodomain of the KNOX protein HAKN1 with DNA. As no structural studies on the interaction

Ngày tải lên: 23/03/2014, 13:20

... C (2000) Interaction of E coli single-stranded DNA binding. .. R (1997) A common core for binding single-stranded DNA: structural comparison of the single-stranded DNA- binding ... protein DnaA and this interaction is essential for DNA replication in Helicob- acter [14,15]. One protein that is central to the DNA replication, repair and recombination is single-stranded DNA binding ... towards ssDNA over dsDNA. M13mp18 ssDNA or pUC18 dsDNA alone or a mixture of both was incubated either in the absence or presence of a different amount of HpSSB followed by separation of the DNA–protein

Ngày tải lên: 30/03/2014, 02:20

... when a new DNA duplex is formed and one of the strands formerly in dsDNA is released as ssDNA Thus, a RecA filament assembles on DNA at the first stage; this process is more efficient with ssDNA Binding ... is formed, the second DNA bind-ing site of RecA can bind dsDNA In addition, ssDNA can be bound there, even more efficiently than dsDNA After strand exchange, the second RecA DNA binding site binds ... coli RecA polymerizes on single-stranded (ss) DNA forming a nucleo-protein filament Then double-stranded (ds) DNA is bound and searched for segments homologous to the ssDNA Finally, homologous

Ngày tải lên: 30/03/2014, 16:20

... MR film R E S U L T S Increased DNA binding ofin vitro generated ERRc in the presence of serum In order to analyze the DNA interaction of ERRc, the full-length cDNA coding for ERRc2 was cloned ... forms dimers even in the absence of DNA Two elements, located in the hinge region and in the LBD, respectively, are necessary for DNA-independent dimerization DNA bind-ing of bacterial expressed ... highly conserved region of these proteins is the DNA-binding domain (DBD, C-domain), which contains two zinc-binding modules that fold to form a single structural domain [5] They confer binding

Ngày tải lên: 31/03/2014, 09:20

... in all eukaryotes where it is required for joining DNA fragments produced during DNA replication DNA ligase also plays important roles in DNA single strand break (SSB) repair pathways in mammals ... double or single strand DNA breaks respectively [19,20] The Comet assay was used here to investigate the kinetics of DNA repair in atlig1-RNAi lines compared to wild-type plants DNA single strand ... in DNA metabolism, with clear differences in the functions of DNA ligase orthologues between animals, yeast and plants DNA ligase 1, present in all eukaryotes, plays critical roles in both DNA

Ngày tải lên: 12/08/2014, 03:20

... Trang 1Open AccessResearch A single site for N-linked glycosylation in the envelope glycoprotein of feline immunodeficiency virus modulates the virus-receptor interaction Brian J Willett*, ... did it increase the affinity of the CXCR4 interaction, suggesting that the principal effect was targeted at reducing the complexity of the Env-CD134 interaction Background The initial event in ... be dependent on intermolecular interactions in the tertiary complex of the native trimeric Env on virions and accord-ingly that the T271I mutation modulates this interaction As an additional control

Ngày tải lên: 13/08/2014, 05:21

... systems requiresstrong interaction between photons and atoms manip-Apart from the quantum information applications, a more fundamental interest insingle atom - single photon interaction is to answer ... discuss the interaction of heralded single photons with an asymmetric cavity as a method to shape the temporal envelope of the singlephotons in order to make them suitable for absorption by a single ... for the preparation of a single photon We present variousexperiments to quantitatively characterize the generated single photons, and ways tomanipulate them for efficient interaction with atoms

Ngày tải lên: 09/09/2015, 08:17

... real-time replication rate of a single DNA polymerase (DNAP) on a single- stranded DNA (ssDNA) stretched by a magnetic trap as shown in Fig 5A Similarly to RNAP, DNAP reads the Fig (A) Schematic ... requirements: (1) the stretched DNA molecules should preserve the base stacking structure of unstretched DNA, thus allowing normal DNA protein interactions; (2) the DNA should be immobilized in ... 186 immobilized DNA than on a coiled DNA, since along the former the protein follows a straight 1D path along the DNA Stretching DNA is also useful if one wishes to monitor its interactions with...

Ngày tải lên: 22/06/2014, 18:20

... Joung JK, Dobbs D: ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool Nucl Acids Res 2010, 38:W462-468 35 Mandell JG, Barbas CF: Zinc finger tools: custom DNA- binding domains for ... file 2, are protein motifs capable of targeted DNA- binding [34-36] Each individual zinc finger usually recognizes three nucleotide bases, but many zinc fingers can be combined to generate an array ... are presented for the first time Results Identification of HSV-2 genome specific zinc finger array (ZFAs) and zinc finger nucleases (ZFN) Using the FASTA format of the nucleotide sequences of the...

Ngày tải lên: 13/08/2014, 16:20

... DNA as Polymer DNA is a macromolecule consisting of numerous basic repetitive units (DNA backbones) These repetitive units furthermore form the DNA double helix structure The doublestranded DNA ... Concentration on the Affinity of NAP-1 to Histone Octamers 42 CHAPTER SINGLE MOLECULE STUDY ON MVAT/MVAU -DNA AND MVAT MUTANTS -DNA INTERACTIONS 44 ii 3.1 INTRODUCTION 45 3.2 ... iii Abstract Single molecule manipulation techniques have become versatile tools to study the force and motions of biological molecules such as DNA and proteins, and DNA- protein interaction in...

Ngày tải lên: 12/10/2015, 17:34

... discrimination rendered by the presence of a single mismatch in the DNA on the Fig ATP induced aggregation of MutS-MutL in presence of homo ⁄ heteroduplex DNA MutS-MutL -DNA complexes were formed by incubating ... duplex DNA) were heat denatured with SDS loading buffer, analysed by 10% SDS ⁄ PAGE, followed by silver staining to visualize both proteins and DNA [(C) Heteroduplex DNA (D) homoduplex DNA; lane ... analysis of Rh changes following DNA addition seems to suggest that MutS interaction with heteroduplex DNA rapidly generates a particle size similar to that of homoduplex DNA (14 nm) following which...

Ngày tải lên: 07/03/2014, 12:20

... mutant mice by gene expression 4242 analysis This approach identified the Promyelocytic Leukemia Zinc Finger (PLZF) gene as a putative gene target of CUX1 PLZF was originally identified as a t(11;17) ... a repression domain ` BTB ⁄ POZ (bric-a-brac, tramtrack, brad complex ⁄ poxvirus zinc finger) and nine Kruppel zinc fingers [20] ¨ This repression domain allows PLZF to recruit corepressors such ... triplicate ChIP assays Genomic DNA amplification, reporter plasmid constructs and mutagenesis The 3-kb 5¢-UTR region of the human PLZF gene was amplified by PCR from purified genomic DNA isolated from the...

Ngày tải lên: 29/03/2014, 21:20

... mhlblt DNase I digestion throughout the DNA fragment, this could be the result of nonspecific interaction with DNA or direct inhibition of the enzyme itself A major problem with using DNase I ... (9) A single ActD molecule would thus cover about bp of DNA However, DNase I has a small loop, important to the binding process, that fits mto the minor groove of DNA, and spans 3-4 bp of DNA (20) ... binding to DNA causesthe cleavage agent, DNase I, to redistribute to DNA sites not blocked by bound drug (11) The DNase I footprmtmg experiments were carried out in the presence of calf thymus DNA as...

Ngày tải lên: 11/04/2014, 09:40