ku binds dna ends in a sequence independent manner

... unspliced pgRNA and the SP1 splicing variant of HBV, primers SP1 (tgcccctatcctatcaacac), SP2 (actcccataggaattttccgaaa) and U2 (ttccaatgaggattaaagacag) were used Quantification of the splicing ratio by ... identified ISSs are S-AS ISSs, suggesting that the splicing regulatory mechanism associated with the sequence- independent S-AS repression is general in maintaining intact HBV transcripts The alignment ... proteins, a family of proteins containing one or two RNA-binding domains and a signature RS domain rich in Arg ⁄ Ser dipeptides, and splicing silencers usually recruit heterogeneous nuclear RNPs, a set...

Ngày tải lên: 28/03/2014, 23:20

... C A GGAC T G G G A G A A A A A AT T TA A G AT C T Mut C AT T T G A A C T G T G C C A AT G A G A G G A G A A A A A AT T TA A G AT C T Mut D AT T T G A A C T G T G C C A AT G C T G A A G G A A A ... AT C T AT T T G A A C T A G A C C A AT G C T G G G A G A A A A A AT T TA A G AT C T Mut A AT T T G A A C T G T G A A G AT G C T G G G A G A A A A A AT T TA A G AT C T Mut B AT T T G A A C T G ... 5¢-GGCGGATCCAAGCCAGTGGTTGTTAAT AC-3¢ and 5¢-GCCTCGAGAATCAAGTGTCCCTGCACC T-3¢ (LIN-54-DN); 5¢-GCGGATCCGAGGTGGTGCCAG CTGAG-3¢, 5¢-GCTCTAGAGAATGGAAGCCGTGCCT G-3¢, 5¢-GCTCTAGATTGGCAGATGCAGCTGAAGTA3¢ and...

Ngày tải lên: 23/03/2014, 04:20

... lanes, caused, for example by unequal amounts of DNA present in each lane or by smiling effects RESULTS DNA extraction and analysis cpDNA The method of total DNA extraction and analysis of cpDNA ... rDNA fragments Indeed, the presence of size markers within a lane enables a more accurate estimate of fragments in that lane than in other lanes, since there is often at least a slight shift among ... variation at all to extreme cases with up to 20 variants per plant in Vicia faba (Rogers and Bendich, 1987) and a great deal of within-population variation In oaks, Bellarosa et al (1990) found that...

Ngày tải lên: 08/08/2014, 19:21

... TCGACGTGTCGAAGTGAAAG; AtpA1170R: TCTGAGCCAAATTGAGCAAA) DNA nucleotide sequences of the cox1 and atp1 coding regions were determined for the maternal plants and a few representative progeny from each family ... study Within-sibship variation was attributable to paternal inheritance, lineage sorting or maybe to the rearrangement of mt DNA by recombination Our Page of 11 results demonstrate that transcription ... PCR-RFLP haplotype was not present in maternal plant even in trace amount, and if (3) it differed in all mt markers analyzed, which made paternal inheritance more parsimonious explanation than substoichiometric...

Ngày tải lên: 12/08/2014, 03:21

... listing of Tax binding proteins with accompanying literature citations can be found by visiting the publicly accessible Tax website http://htlv-tax.com As data accumulates regarding Tax-protein interactions, ... knowledge gained in existing literature that defined a physical interaction between Tax and a cellular protein, to comprise an in silico Tax interactome This interactome was then restricted to proteins ... defects in the cellular DNA damage repair response Results Assimilation of an interaction database for Tax We conducted a manual literature search for articles with reference to "Tax Interaction"...

Ngày tải lên: 13/08/2014, 05:21

... non-coating DNA (i.m), gene gun administration using gold-coated DNA and gene gun administration using non-coating DNA Our results indicated that the routes of administration and formulation of DNA clearly ... potential application of EGFR DNA vaccine in the further clinical trials It was interesting to observe that administration route and forms of DNA affected the CTL activity in the spleen and inguinal ... transfected epidermal Langerhans cells or antigen loaded epidermal Langerhans cells moving into draining inguinal lymph nodes [25-27] By contrast, intramuscular injection of DNA initiated mainly...

Ngày tải lên: 14/08/2014, 19:22

... adsorption on a surface.[151,159] Fang et al have shown that ethanol can induce DNA condensation in the presence of divalent magnesium only, but, again, a DNA solution was incubated with ethanol ... the DNA attraction DNA binding is then dependent on the fractional surface densities of the divalent and monovalent cations, which can compete for the mica surface and DNA neutralizations In addition, ... analyzing the properties of long biopolymer chains such as DNA Different architectures have been designed, including entropic trap arrays, micro and nano-pillar arrays, nano-pores and nanochannels...

Ngày tải lên: 14/09/2015, 14:01

... Ichikawa-Iwata, E., Sasaki, M., InoueBungo, T., Shiina, M., Kimura, K., Takata, S., Fujikawa, A. , Morii, H., Kumasaka, T., Yamamoto, M., Ishii, S & Ogata, K (2002) Mechanism of c-Myb-C/EBP beta cooperation ... building and mutational analysis Protein Eng 4, 891–901 Ogata, K., Morikawa, S., Nakamura, H., Sekikawa, A. , Inoue, T., Kanai, H., Sarai, A. , Ishii, S & Nishimura, Y (1994) Solution structure of a ... determination of reporter activation using both a 5-bromo-4-chlorindol-3-yl b-D-galactoside overlay and a liquid b-galactosidase assay (Fig 3) Similar analysis of several subdomains of c-Myb revealed...

Ngày tải lên: 21/02/2014, 00:20

... using a c-scintillation counter (LKB Wallace 1282 Compugamma, Finland) Determination of intracellular iron distribution using native-PAGE-59Fe-autoradiography Native-PAGE-59Fe-autoradiography was ... ferritin and Sper were obtained from Sigma SperNO was obtained from Cayman Chemicals Eagle’s minimum essential medium (MEM) was obtained from Gibco BRL DFO was obtained from Novartis Pharmaceutical ... work was supported by an Australian Research Council Large Grant and Grants 970360 and 981826 from the National Health and Medical Research Council of Australia REFERENCES Moncada, S., Palmer,...

Ngày tải lên: 08/03/2014, 22:20

... Arthritis Research & Therapy Vol No Saadat et al Table Table Proteoglycan content in cartilage of cyclically loaded rabbit metacarpophalangeal joints and their contra-lateral controls Collagen ... 1):345-351 40 Maroudas A, Bayliss MT, Uchitel-Kaushansky N, Schneiderman R, Gilav E: Aggrecan turnover in human articular cartilage: use of aspartic acid racemization as a marker of molecular age Arch ... Racemization of aspartic acid in human articular cartilage Connect Tissue Res 1992, 28:161-169 39 Bank RA, Bayliss MT, Lafeber FP, Maroudas A, Tekoppele JM: Ageing and zonal variation in post-translational...

Ngày tải lên: 09/08/2014, 08:22

... preferential binding to globally cis-platinated DNA [31,33,34] Binding of p73 proteins to the recognition elements is affected by DNA cis-platination in a similar way to p53 binding In agreement ... a clinically used anticancer agent [26,27] The drug binds covalently to DNA, forming several kinds of adduct, among which the most abundant are intrastrand crosslinks (IACs) between neighboring ... was treated with cisplatin in the same way, the intensity of the R53 band on the autoradiogram increased with the degree of DNA modification The increase was already significant at rb ¼ 0.02 At...

Ngày tải lên: 19/02/2014, 05:20

... CGCCAGGGAGCTCACATGCCGTT), and three 3¢ oligonucleotides (primer 3, GAAAAGCTTCAGCTGGAA GTTGAACGGCAT; primer 4, AACAAGCTTCACGAA ATCTCCCAGGTCCAC; primer 7, AACAAGCTTGA AATCTCCCAGGTCCACGGT) were used To facilitate cloning ... quinquefasciatus using Bacillus sphaericus: results of a pilot project in a large urban area of equatorial Africa Bull World Health Organ 71, 367–375 Kumar A, Sharma VP, Thavaselvam D, Sumodan ... possible polyadenlyation signals, a consensus AATAAA and a variant AATTAG (Fig 3, in boldface) In the various RACE 3¢ ends sequenced, four different polyadenylation sites were found (Fig 3, arrows)...

Ngày tải lên: 19/02/2014, 07:20

... 4229 Nagasawa, K., Kitamura, K., Yasui, A. , Nimura, Y., Ikeda, K., Hirai, M., Matsukage, A & Nakanishi, M (2000) Identification and characterization of human DNA polymerase b2, a DNA polymerase ... purification and characterization Chromosoma 102, 631–636 Mizushina, Y., Kamisuki, S., Kasai, N., Shimazaki, N., Takemura, M., Asahara, H., Linn, S., Yshida, S., Matsukage, A. , Koiwai, O., Sugawara, ... Kimura, S., Suzuki, T., Yanagawa, Y., Yamamoto, T., Nakagawa, H., Tanaka, I., Hashimoto, J & Sakaguchi, K (2002) Characterization of plant proliferating cell nuclear antigen (PCNA) and flap endonuclease-1...

Ngày tải lên: 07/03/2014, 15:20

... of the signal sequence Tetracycline-resistant prlA+ and prlA4 Table Bacterial strains and plasmids used in this study Camr and Ampr indicate resistance to chloramphenicol and ampicillin, respectively ... Dynamics) after scanning of the autoradiogram In vitro transcription, translation, targeting and cross-linking analysis To generate truncated mRNA, plasmids encoding truncated nascent chains of ... facilitates the insertion of inner-membrane proteins in addition to catalyzing protein translocation [51,52] Membrane protein insertion and protein translocation are two distinct processes that...

Ngày tải lên: 08/03/2014, 09:20

... ACCCGGGTCG ACTCAGTGATGGTGGTGATGGTGTCCTCGACC AAAAAGATC; rcpA: forward, GCGATAGAATTCATG AGCGTAGAAACGGAAGAC and reverse, CGAAGCTT GTCGACTCAGTGATGGTGGTGATGGTGCTCCG ACGGCAATGTCG; cphB: forward, GCGATAGAATTC ... thus principally allowing covalent chromophore binding Forward primer: 5¢-CACTCGGTACTCCGCAGCGTTT CGCCGTGTCACATTGAATATTTGCACAATATGG -3¢; reverse primer: 5¢-CCATATTGTGCAAATATTCAA TGTGACACGGCGAAACGCTGCGGAGTACCGAG ... forward, GAGGCTGAATTCATGGTAGGAAACGCTACTCAAC; reverse, CGAAGCTTGTCGACTCAGTGATGGTGGT GATGGTGACCCATCTCAGGAAGTACAAC 2664 H J M M Jorissen et al (Eur J Biochem 269) Total RNA extraction, cDNA synthesis...

Ngày tải lên: 08/03/2014, 23:20

... GCCGGGATCCATGGGCAAAACGCTGAGCAAAGAGGACAAGCTCGAG GCCGGGATCCATGGGCAAGCAGAATAGCGCACTGCGGCCAGACAAG GCCGGGATCCATGGGCAAGGCAGCATCTGCAGCAGCAGCAGACAAGCCTGTAGCC GCCGGGATCCATGGGCAAGGCAGCATCTAAGGCAGCAGCAGACAAGCCTGTAGCC AATTAACCCTCACTAAAGGG ... ATATGGATCCATGGCTGCGGCAGCAGCGGCAGCAGCAGCAGACAAGCCTGTAGCC ATATGGATCCATGGGCGCGGCAGCAGCGGCAGCAGCAGCAGACAAGCCTGTAGCC GCCGGGATCCATGGGCGCAGCAGCATCTGCAGCAGCAGCAGACAAGCCTGTAGCC GCCGGGATCCATGGGCGCAGCAGCANNKGCAGCAGCAGCAGAC ATATGGATCCATGGGCNNKGCAGCAGCGGCAGCAGCAGCAGAC ... ATATGGATCCATGGGCNNKGCAGCAGCGGCAGCAGCAGCAGAC GCCGCTCGAGCCTGTAGCCCATGTT AATTCTCGAGTGCTGCTGCTGCCGATGCTGC AATTCTCGAGTGCTGCTGCTGCCGTTGCTGC AATTCTCGAGTGCTGCTGCTGCGAATGCTGC GCCGGGATCCATGGGCAAAACGCTGAGCAAAGAGGACAAGCTCGAG...

Ngày tải lên: 16/03/2014, 16:20

... creatine kinase, 0.25 mgÆmL)1 heat treated BSA, and lCi [a- 32P]dCTP and lCi [a- 32P]dTTP (each 3000 CiÆmmol)1, AmershamBiosciences) Recombinant DNA polymerase a- primase was added as indicated After ... [a3 2P]dCTP and [a3 2P]dTTP (3000 CiÆmmol)1, AmershamBiosciences) DNA polymerase a- primase was added as indicated The incorporation of radioactive dNMP was measured by acid-precipitation of DNA and scintillation ... support DNA replication of a plasmid carrying the JCV origin Columns and 3, SV40 Tag with human and murine DNA polymerase a- primase, respectively; columns and 5, JCV Tag with human and murine DNA polymerase...

Ngày tải lên: 17/03/2014, 03:20

... oxidative damage occurring and being maintained in the mitochondrial genome The levels of oxidatively damaged bases in mtDNA are 10- to 20-fold higher than that observed in nuclear DNA [12,13] Also, ... Cortopassi, G .A. , Shibata, D., Soong, N.W & Arnheim, N (1992) A pattern of accumulation of a somatic deletion of mitochondrial DNA in aging human tissues Proc Natl Acad Sci USA 89, 7370–7374 24 Katayama, ... These data clearly demonstrate that ETS abnormalities have a localized physiological impact on the cell and can result in fiber atrophy Subsequent longitudinal analysis of atrophied fibers also showed...

Ngày tải lên: 17/03/2014, 23:20

... of PAR4 [27] Thrombin has been shown to cleave and activate PAR1, PAR3 and PAR4, whereas trypsin cleaves and activates PAR2 As duodenase is capable of cleaving certain substrates with trypsin-like ... pathways has a modulatory rather than a mandatory role to play in mediating the proliferative response In contrast, a recent report has shown that tryptase induces DNA synthesis in canine tracheal ... PAR3 and PAR4 [11] Interestingly, thrombin has now been demonstrated to cleave and activate PAR1, PAR3 and PAR4 whereas trypsin and tryptase activate PAR2 [12] Certain other proteases, including...

Ngày tải lên: 24/03/2014, 03:21

... dass Jan [sein Fahrrad]b reparierte ja et Peeter oma jalgratta puhastas und dass Peter sein Fahrrad putzte (20) Jan parandas oma uue jalgrattab Jan reparierte sein neues Fahrradb ja tema vennad ... Jan fachkundig sein Fahrrad reparierte ja [et Jan]f hoolikalt [oma jalgratta]f puhastas und [dass Jan]f eifrig [sein Fahrrad]f putzte (17) * Jan parandas oma jalgratta asjatundlikult * Jan reparierte ... ja [et Jan oma jalgratta]f hoolikalt puhastas und [dass Jan sein Fahrrad]f eifrig putzte and that Jan his bike diligently cleaned (16) *… et Jan asjatundlikult oma jalgratta parandas dass Jan...

Ngày tải lên: 31/03/2014, 20:20