in vivo insulin infusion kinetics

... liver insulin signaling, and that it may represent an interesting molecule with which to regulate liver insulin action Experimental procedures Materials Porcine insulin, recombinant human insulin, ... effect on IR kinase and insulin signaling [10] The interaction of Grb14 with PDK-1, an upstream kinase that activates Akt in response to insulin, appears to be required for insulin- induced membrane ... Desbuquois et al Insulin- induced translocation of Grb14 in rat liver A H N B M LP S PM GE –ins Grb14 – ins Grb14 + ins –ins + ins + ins – ins EEA1 + ins Na+K+ATPase Calnexin + ins number: 10 11...

Ngày tải lên: 18/02/2014, 18:20

... prostaglandin D2 in adipogenesis in vivo Y Fujitani et al indicate that overexpression of H-PGDS increases insulin sensitivity in vivo Adipocyte differentiation ex vivo Finally, we examined whether ... improvement in peripheral insulin resistance through the activation of PPARc in WAT, it is possible that PGD2 stimulates pancreatic islets to increase insulin secretion Indeed, serum insulin levels ... drugs to improve insulin sensitivity [37] Indeed, the overexpression of H-PGDS improved insulin resistance (TG mice showed clear hypoglycemia in response to insulin clamp, as shown in Fig 4B) Thus,...

Ngày tải lên: 16/02/2014, 09:20

... of insulin action but not insulin signaling in human skeletal muscle is reduced by exercise Diabetes 2002, 51:583-590 69 Yu S, Castle A, Chen M, Lee R, Takeda K, Weinstein LS: Increased insulin ... or 5% bovine serum albumin (BSA) in TBST Incubation with primary antibody anti-PTBP1 (Polypyri­ midine tract-binding protein 1; Proteintech Group Inc (Chicago, Illinois, USA) at 1:1,000 in 5% skimmed ... human microarray studies examining insulin resistance in skeletal muscle paint a remarkably similar picture - one of no striking change in protein coding mRNA abundance In the Patti et al study [2],...

Ngày tải lên: 11/08/2014, 12:20

... discrimination level normal tissue 0 100 200 300 400 500 time [min] Values of PPIX-fluorescence in normal findings, findings ≤ mild dysplasia and findings ≥ moderate dysplasia plotted against the ... Met + No + Hyp + Inf + Inf + Hyp Hyp + Dys II + Sqc + Sqc + TBC + Sqc + Sqc + Ade + Hyp + Hyp Sqc + Inf + Sqc + Ade + Met Inf Hyp + Dys II + Inf Dys III + Sqc + No + Inf + Inf Inf - Fluorescence ... application and spectroscopy Values of PPIX-fluorescence in normal findings, findings ≤ mild dysplasia and findings ≥ moderate dysplasia plotted against the time between 5-ALA application and spectroscopy...

Ngày tải lên: 12/08/2014, 15:20

... den Berghe G: Survival benefits of intensive insulin therapy in critical illness: impact of maintaining normoglycemia versus glycemia-independent actions of insulin Diabetes 2006, 55:1096-1105 Su ... difference in organ function Secondly, it remains obscure whether a change in forearm flow is giving any information on the vital organ perfusion and oxygenation Indeed, an increase in blood flow ... http://ccforum.com/content/14/1/122 The clinical relevance of a reduction in forearm flow in patients with severe sepsis with intensified insulin therapy remains unclear, as recognized adequately by...

Ngày tải lên: 13/08/2014, 20:21

... truyền insulin Chuyển từ insulin truyền tĩnh mạch sang tiêm da Khi bệnh nhân bắt đầu ăn đường huyết ổn định, nên chuyển insulin tĩnh mạch sang đường tiêm da phác đồ nhiều mũi (insulin nền, insulin ... bữa ăn, insulin hiệu chỉnh) Vì thời gian bán huỷ insulin tiêm tĩnh mạch ngắn nên insulin tiêm da nên chích 1-2 trước ngưng truyền tĩnh mạch Liệu pháp insulin tiêm da • Tính tổng liều insulin hàng ... insulin truyền tĩnh mạch sang insulin tiêm da PROTOCOL INSULIN TRUYỀN TĨNH MẠCH Pha truyền insulin • Dùng Insulin regular human (Actrapid, Humulin R, Wosulin R) T1/2 = 5-9 phút sau vào thể •...

Ngày tải lên: 23/07/2015, 21:11

... characterized by insulin resistance and insulin secretion deficiency At present, there is no a single medication which treats type diabetes by improving both insulin resistance and insulin secretion ... glycine-rich linker is flexible, which allows the specific engineering of hinge regions into proteins to achieve desired functional motions [17] We used a glycine-rich short peptide including 15 amino ... promising agent that can treat type daibetes by improving both insulin resistance and insulin secretion deficiency However, we only observed the glucose-lowering effect of the whole fusion protein...

Ngày tải lên: 25/10/2012, 11:10

... anti-inflammatory activity is measured by the model of carrageenin-induced edema in hind paw of the rat Results: 10 Polyoxychalcones were obtained with good yields and the results of in vivo ... affect to the in vivo anti-inflammatory activity of tested chalcones The polyoxychalcone which possessing a hydroxy group at position C2 of A ring and at least one group methoxy in B may be consider ... nhóm methoxy vòng B làm tăng tác dụng kháng viêm in vitro in vivo chalcon - Nhóm 2’-hydroxy có ảnh hưởng định đến tác dụng kháng viêm in vitro in vivo chalcon Tăng tác động kháng viêm cho chalcon...

Ngày tải lên: 15/11/2012, 14:03

... were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study ... i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats The plasma ... novel hydrophobized polysaccharide/oncoprotein complex vaccine induces in vitro and in vivo cellular and humoral immune responses against HER2-expressing murine sarcomas Cancer Res 58:3385–90 Gustafson,...

Ngày tải lên: 23/04/2013, 21:38

... triphosphate BAP : 6-Benzylaminopurine CV : Correlation of Variants D : DMSO : Dimethyl sulphoxide EMS : Ethyl methane sulphonate G : G ch Ki : Kinetin (6-Furfurylaminopurine) LSD : Least Significant ... tớnh nụng sinh h c c a m t s dũng hoa ng Ti n (Gerbera jamesonii) t bi n t o c nuụi c y in vitro v in vivo 1.2 M c ớch v yờu c u c a ti 1.2.1 M c ớch ỏnh giỏ c cỏc c tớnh nụng sinh h c c ... 47 Bi u 6: Kh nng sinh tr ng v nhõn nhanh c a cỏc d ng ch i ng Ti n t bi n in vitro (gi ng T) 50 Bi u 7: Kh nng sinh tr ng v nhõn nhanh c a cỏc d ng ch i ng Ti n t bi n in vitro (gi ng D)...

Ngày tải lên: 14/12/2013, 16:09

... experiment The subsequent incubation with the indicated retinoids was then terminated at the indicated time points by washing with NaCl ⁄ Pi and lysis of the cells using mL of Trizol (Invitrogen) per well ... stained in Alcian blue dye (Sigma-Aldrich, Steinheim, Germany) solution [0.5 g of dye in 500 mL of 70% (v ⁄ v) ethanol containing 1% HCl], differentiated in acidic ethanol (70% ethanol containing ... this metabolite in an in vivo model To this end, we used the developing chick wing bud model, a classical model to measure RA action In this model, at-RA induces digit duplications in a dose-dependent...

Ngày tải lên: 18/02/2014, 08:20

... the integrin-binding motif of BchH, simultaneously triggering porphyrin metallation This would also lead to a release of the blockade of the ATP-binding site of BchI by the integrin I domain, ... hydrolysis In addition, kinetic analysis had shown binding of Mg2+ to this subunit [21] An integrin I domain, suggested to exist in the C-terminus of the 70 kDa subunit, is a metal binding site ... AAA+ protein In the doubleringed BchI-BchD structure the ATPase activity of BchI is blocked A conformational transition upon binding to BchH may bring the integrin I domain of BchD into contact...

Ngày tải lên: 19/02/2014, 12:20

... that removal of the N-terminal helices and while leaving the interacting a-helix intact, may destabilize the proteins leading to spurious protein–protein or/and protein–DNA interactions The crystal ... mobility binding assays with increasing concentrations of control Ydc2 and Ydc2-ND35 (A) or Ydc2-ND35/CD15 (B), as indicated, visualized by phosphorimaging (C) Binding reactions with increasing concentrations ... demonstrated [38] suggesting that recombination intermediates and enzymes that resolve them could be involved in maintaining the stability and inheritance of mitochondrial genomes in human cells These...

Ngày tải lên: 20/02/2014, 11:20

... UP12 is a stress protein DISCUSSION In this work, we have identified two proteins GatY and UP12 as putative in vivo substrates of the chaperonin GroEL In addition to its in vivo interaction with GroEL ... chaperonin proteins in protein folding in vivo FASEB J 10, 5–9 26 Ewalt, K.L., Hendrick, J.P., Houry, W.A & Hartl, F.U (1997) In vivo observation of polypeptide flux through the bacterial chaperonin ... chromatography using His-bind resin (Novagen), according to the manufacturer’s instructions The protein was eluted with buffer K containing M imidazole and cleaved by thrombin (Novagen) at room...

Ngày tải lên: 22/02/2014, 07:20

... required [12] In this minireview, major factors in determining overall RNAi efficiency in vivo are introduced Moreover, up-to-date progress in achieving efficient and targeted siRNA delivery in vivo, particularly ... successfully inhibited the expression of VEGF, thereby suppressing the growth of hepatocarcinoma tumors in mice [87] In addition, no noticeable increase in inflammatory cytokines, including interferon-a ... obtained by in vitro transcription reaction and, therefore, avoid contamination by cell or bacterial products Promising in vitro and in vivo RNAi was obtained using siRNA that was directly linked...

Ngày tải lên: 06/03/2014, 01:23

... enzyme of interest should be investigated in the context of the in vivo- like medium Finally, in vivo, the enzymes are present at much higher concentrations than in typical enzyme assays, in which ... obtained in the in vivo- like assay medium, suggesting that the ‘optimized’ conditions are, in reality, not optimal for these enzymes A thorough analysis of the yeast kinetics of phosphofructokinase ... at least three independent cell-free extracts from steady-state samples from a single chemostat culture Enzyme In vivo- like Vmax (mmolÆmin)1Æg protein)1) Flux (mmolÆmin)1Æg protein)1) HXK PGI...

Ngày tải lên: 06/03/2014, 09:22

... protein, in binding porphyrins It is not known how haem is transported intracellularly in bacteria, and the mechanisms by which haem is incorporated into soluble and membrane-bound proteins inside ... transiently bound to specific protein factors in cells Little is known about intracellular haem transport and how haem is incorporated into proteins in vivo to form haem proteins in various subcellular locations ... compounds being taken up into the cell and incorporated into vital haem proteins instead of haem [5] We show here that haem analogues are indeed taken up and can be incorporated into haem proteins,...

Ngày tải lên: 06/03/2014, 11:20

... function of the increasingly large group of proteins that have been shown to contain intrinsically unstructured regions The intrinsic conformation flexibility that is inherent in this binding mechanism ... min), in strain YPP310 (light grey bars, DDABDswi1 + snf5) lacking both activator-binding domains (SWI1D329-655, SNF5D2-327), strain YPP211 (white bars, DABDsnf5) lacking the Snf5 activator-binding ... activator-binding domains is also of interest in relation to the twostep binding mechanism between Gal4 and Swi1 that has been characterized in vitro [34], and our results suggest that the coupled binding...

Ngày tải lên: 07/03/2014, 00:20

... injected in the gonad with 100 lL of saline solution containing dsRNA, or saline solution for the control After dsRNA injection, oysters were maintained in raceways in conditions allowing optimal ... Effects of in vivo oyvl-dsRNA injection on germ cell development in oysters, month postinjection (A) Female control, injected with saline solution Oocytes are in vitellogenesis (B) Female injected ... To validate the in vivo dsRNA injection method in oyster, we used an original technique that consisted of monitoring, by in situ hybridization, the administration of digoxygenin-labelled (DIG-labelled)...

Ngày tải lên: 07/03/2014, 00:20

... treatment To investigate whether HU induces an increase of the Hat1 protein, we used a yeast strain expressing a hemagglutinin (HA)-tagged version of Hat1 The Hat1–HA protein level did not increase ... by the yeast HAT-B complex in vivo Remarkably, the inability of the HAT-B complex to acetylate histone H4 containing the K12R substitution, both in vivo and in vitro, indicates a sequential order ... that an interaction of histone H4, beyond its N-terminus, with the HAT-B complex is needed in order to establish a physiological acetylation pattern Interestingly, in line with this idea, in vitro,...

Ngày tải lên: 07/03/2014, 05:20