chip and real time pcr analysis of chip dna

... with primers for standard PCR amplifica-tion Electrophoresis verified all PCR products were specific with single bands in the gel (Fig S1) The melt-ing profiles of all amplified candidate reference ... ciliatathrough gene expression analysis Results Primer specificity, amplification efficiency, and expression profiles of candidate reference genes Reverse-transcribed cDNA from each sample was used ... Open AccessSelection and validation of reference genes for measuring gene expression in Toona ciliata under different experimental conditions by quantitative real-time PCR analysis Huiyun Song1,2,3,...

Ngày tải lên: 28/06/2021, 20:42

... control to ensure the quality of RNA and cDNA PCR products amplified from cDNA are indicated by asterisks Sequences for the cDNAs were confirmed by cloning and sequencing PCR products at higher molecular ... (miR159a and miR159b) have been sequenced more than 5,000 times in mature pollen Since the expression of these two miRNAs was not detected by either real-time PCR or regular RT-PCR (Table 1 and Figure ... each primers (900 nM), 1 μl Taqman probe (250 nM), and 1.5 μl cDNA sample All real-time PCR reactions were performed in a StepOne real-time PCR machine (Applied Biosystems, Foster City, CA) with...

Ngày tải lên: 12/08/2014, 03:20

... elastic deformations of soft Trang 29Simula-organs and tissues with both high visual realism and real-time performance utilizingtetrahedral meshes of varied resolutions.In Chapter 4 of the dissertation, ... domain of virtual reality (VR) The use of virtual realitytechnology opens new realms in the teaching, practice, and investigation of medical Trang 26med-(a) (b)Figure 1.5: (a) Volume rendering of ... analyzed, and utilized tomodel specific medical procedures Figure 1.5 shows examples of a volume-renderedand a surface-rendered torso of a patient Producing realistic data models and providing real-time...

Ngày tải lên: 13/11/2014, 16:25

... đại IV.3 Kỹ thuật real time PCR định lượng HBV DNA Kỹ thuật real time PCR định lượng hay còn gọi là PCR động đã cho phép hiển thị và theo dõi trực tiếp quá trình nhân bản DNA đang diễn ra theo ... thường dựng để định lượng vi rỳt viờm gan B là Real Time PCR (Khuếch đại DNA đớch với thời gian thật) và bDNA (Khuếch đại tớn hiệu) Trong đó, kỹ thuật real time cho kết quả chính xác, độ nhạy và độ ... học phân tử real time PCR định lượng Virut HBV DNA trong huyết thanh bệnh nhân mắc viêm gan siêu vi B Mục đích: 1 Đánh giá độ nhạy và đặc hiệu của quy trình kỹ thuật Real- time PCR định lượng...

Ngày tải lên: 18/02/2021, 16:02

... mảnh DNA cần khuếch đại IV.3 Kỹ thuật real time PCR định lượng HBV DNA Kỹ thuật real time PCR định lượng hay còn gọi là PCR động đã cho phép hiển thị và theo dõi trực tiếp quá trình nhân bản DNA ... HBV DNA trong chẩn đoán Trang 4II Vật liệu nghiên cứu 35 III.1.3 Kỹ thuật định lượng HBV DNA bằng kỹ thuật real time PCR 42 I Đánh giá độ nhạy và đặc hiệu của quy trình kỹ thuật real-time PCR ... học phân tử real time PCR định lượng Virut HBV DNA trong huyết thanh bệnh nhân mắc viêm gan siêu vi B Mục đích: 1 Đánh giá độ nhạy và đặc hiệu của quy trình kỹ thuật Real- time PCR định lượng...

Ngày tải lên: 28/02/2021, 11:44

... này phụ thuộc vio cả DNA-polymerase và chiếu đài mảnh DNA cần khuếch đại 1V.3 Kỹ thuật rcal time PCR dịnh lượng HBV DNA Kỹ thuật real time PCR dinh lượng hay còn gọi là PCR động đã cho phép ... hoc phan tit real time PCR dink luyng Virut HBV ĐÀNA trong huyết thanh bệnh nhản mắc viém gan siéu vi B Mye dich: 1 Đánh giá độ nhạy và đặc hiệu của quy trink ki thudt Real-time PCR định lượng ... thưởng dùng để định lượng vi rút viêm gan B 1a Real Time PCR (Khuếch dai DNA dich voi thoi gian that) va BDNA (Khuéch đại tín hiệu) Trong đó, kỹ thuật real từme cho kết quả chính xác, độ nhạy và...

Ngày tải lên: 12/06/2025, 20:52

... thưởng dùng để định lượng vi rút viêm gan B 1a Real Time PCR (Khuếch dai DNA dich voi thoi gian that) va BDNA (Khuéch đại tín hiệu) Trong đó, kỹ thuật real từme cho kết quả chính xác, độ nhạy và ... hoc phan tit real time PCR dink luyng Virut HBV ĐÀNA trong huyết thanh bệnh nhản mắc viém gan siéu vi B Mye dich: 1 Đánh giá độ nhạy và đặc hiệu của quy trink ki thudt Real-time PCR định lượng ... thưởng dùng để định lượng vi rút viêm gan B 1a Real Time PCR (Khuếch dai DNA dich voi thoi gian that) va BDNA (Khuéch đại tín hiệu) Trong đó, kỹ thuật real từme cho kết quả chính xác, độ nhạy và...

Ngày tải lên: 18/06/2025, 08:19

... oligonucleotides of 1 nM 5.1.2 Mechanisms of PCR synthesis process In order to further understand the mechanisms of PCR synthesis process, the underlining kinetics of PCR gene synthesis and standard PCR ... annealing time, annealing temperature, concentration of oligonucleotides, concentration of dNTPs monomers, and number of PCR cycles The hybridization mechanisms of normal PCR amplification and PCR ... expressed as the half-time constant of the hybridization reaction of a single-stranded DNA (ssDNA) in a mixture, is a function of the number of unique oligonucleotides and the oligonucleotide...

Ngày tải lên: 14/09/2015, 08:48

... Trang 1Real-time PCR quantification and diversity analysis of thefunctional genes aprA and dsrA of sulfate-reducing prokaryotes in marine sediments of the Peru continental margin and the Black ... QUANTIFICATION OF THE FUNCTIONAL GENES aprA AND dsrA OF SRP AND 16S rRNA OF TOTAL BACTERIA IN MARINE SEDIMENT SAMPLES Depth profiles of DNA copy numbers of the functional genes aprA and dsrA as marker ... 106–107copies/g between 0.18 and FIGURE 1 | Depth profiles of DNA copy numbers of the functional genes aprA and dsrA as marker for sulfate-reducing prokaryotes (SRP) and the 16S rRNA gene of total Bacteria...

Ngày tải lên: 04/12/2022, 16:12

... performance of the PCR was monitored by quantitative real-time PCR (qPCR) The mean cycle threshold (Ct) and the standard deviation of the controls were calculated Samples in which the mean Ct of the test ... (λ) DNA and primers Briefly, test sam-ples were 'spiked' into a PCR reaction containing approximately 100 copies of bacteriophage λ DNA and a primer pair directed against λ DNA The performance of ... cycles Standards of 106 to 10 copies per reac-tion of C trachomatis pgp3 amplicons and negative con-trols were included in each PCR reaction to generate a standard curve and quantities of the...

Ngày tải lên: 19/06/2014, 08:20

... were tested for specificity by RT-PCR and also by qRT-RT-PCR, followed by a dissociation curve and agarose gel electrophoresis Real-time PCR conditions and analysis PCR reactions were performed in ... treated with DNAse to avoid misinterpretation of qRT-PCR results by genomic DNA contamination in cDNA samples RNA quality analysis and quantitation were performed by agarose gel analysis and a Nano-Drop ... transferred as a Microsoft Excel file (Microsoft, Redmond, WA) for further gene expression stability analysis Analysis of gene expression stability For analysis of gene expression stability and rank, geNorm...

Ngày tải lên: 12/08/2014, 03:20

... GoTaq® Flexi DNA polymerase (Promega) in a 25 μL reaction containing: 2 μL of cDNA, 2 mM MgCl2, 0.2 mM each dNTP, 0.4 μL of each primer and 1.25 U enzyme Real-time PCR Mitchell Real-time PCR was performed ... CT of 14.8, and SAND Figure 1 Developmental stages of leaves and flowers used for RNA extractions Representative photographs of leaves and flowers of Petunia hybrida lines Mitchell (a, c) and ... basis of tblastx e-values Trang 4the lowest, with a CT of 21.2 In contrast, the highestand lowest expressed genes in V30 were EF1a and ACT, with CTs of 18.3 and 25.1, respectively Analysis of variance...

Ngày tải lên: 12/08/2014, 03:21

... reduction of infectivity of TZM-bl cells was measured as a function of the degree of IgG1b12 mediated neutralization of these Envs association of autologous neutralization sensitivity of patient ... 11, IVC and and IVC and 11 their sensitivity to sCD4 and contemporaneous plasma antibodies Thus, Envs 2-3.J18, 2-5.J3 and 2-5.J11 which showed the highest neutralization sensitivity (IC50 of 0.5, ... dependence of patient Envs with their sensitivities to autologous plasma antibodies and sCD4 Association of CD4 usage of Env-pseudotyped viruses with autologous plasma antibodies and (P < 0.0155) and...

Ngày tải lên: 13/08/2014, 01:20

... capable of generating arsine in the presence of borohydride The arsenic content in rice and chicken is of the same order of magnitude and about three and two orders of magnitude lower than that of ... 10 ml of concentrated HNO3 were added and the reactor was covered and pre-digested overnight Next, 20 mg of Na2 S2 O8 and ml of HClO4 (or 0.3 ml of concentrated HF in rice) were added and heated ... powder candidate reference materials (prepared within the framework of a European project) of environmental and biological origin: rice, chicken, fish and soil samples Sample preparation of lyophilized...

Ngày tải lên: 15/03/2014, 23:49

... of human ESR1 3¢-UTR) in human and other mammalian species, including the chimpanzee, rhesus monkey, mouse, rat, dog and rabbit (Fig 2A) The predicted DG of 70 bp 5¢- and 3¢-flanking regions of ... luciferase activity of pGL3m–ESR1–3¢-UTR–WT (ESR1–3¢-UTR–WT) and pGL3m–ESR1–3¢-UTR–MUT (ESR1–3¢-UTR–MUT) in HEK293T and MCF-7 cells cotransfected with pcDNA-3.0–miR-22 or pcDNA-3.0 and synthetic miR-22 ... is the median; the upper and lower limits of the box are the 75th and 25th percentiles, respectively, and the vertical bars above and below the box indicate the maximum and minimum values The solid...

Ngày tải lên: 22/03/2014, 21:20

... Surviving clones were then expanded in the same medium and analyzed for the expression of TRPM4b by RT -PCR and western blotting RT -PCR Total RNA was isolated, and RT -PCR was performed as described ... Quantitative analysis of the depolarization of the plasma membrane 200 s after addition of ionomycin The results of a western blot experiment showing the different levels of expression of TRPM4b ... untreated (dashed lines) (B) Quantitative analysis of the amplitude of Ca2+ released by ionomycin (left) and the amplitude of Ca2+ entry after readdition of Ca2+ (right) Values for the Ca2+ influx...

Ngày tải lên: 23/03/2014, 09:21

... 50% of a group of test animals Mucus membrane and eye toxicity test: The conjunctiva of the eye and the vaginal vault of experimental animals (rabbits and monkeys) have been employed in tests of ... Tecnología Agraria y Alimentaria is the analysis of pesticides and other contaminants in food and environmental matrices and the evaluation of exposure to biocides and existing chemicals He has published ... leaves It is used for the control of storage roots of fruits and vegetables and it is effective for seed dressing of cereals Table 1.22 summarizes the properties of various frequently used fungicides...

Ngày tải lên: 12/08/2014, 04:22

... risk of exposure of the chemist to toxic solvents and vapors always exists From a practical point of view, the formation of emulsions, which are sometimes difficult to break up, the handling of ... use of solvent mixtures (especially for the extraction of pesticides of different polarity) combining the ability of heating of one of the components (i.e., acetone) with the solubility of the ... liquids The efficiency of the process depends on the affinity of the analyte for the solvents, on the ratio of volumes of each phase, and on the number of successive extractions Most of the LLE applications...

Ngày tải lên: 12/08/2014, 04:22

... using the latest concepts and techniques, better understanding and control of the combination of hapten molecules and macromolecular carriers, and improving the efficiency of ß 2007 by Taylor & Francis ... detection of organophosphate pesticides and nerve agents Anal Chem., 78, 835–843, 2006 78 Minunni, M and Mascini, M Detection of pesticide in drinking water using real- time biospecific interaction analysis ... determined and documented Triplicate analyses of each standard, control, and sample are generally performed for 96-microwell plate assays The %RSD of measured concentrations from triplicate analysis...

Ngày tải lên: 12/08/2014, 04:22

... frequency (time or distance), spacing, mixing, dividing of samples, and consequently the time required for sampling and the cost of sampling, shipping, and analysis of samples Careful balancing of cost ... safety, and security precautions Determination of the time required for reaching the sampling site and handling the samples Provisions for prevention of contamination and deterioration of samples ... residue and the average CV of the residues For instance, even if 30 pairs of random composite samples of size 10 were withdrawn 100 times from a data population having a CV of 0.28, the minimum and...

Ngày tải lên: 12/08/2014, 04:22