cd detected and structure characterization

... donor (TLL tryptophans) and an acceptor (fatty acid) is that there must exist a spectral overlap between the donor emission and the acceptor absorption spectra, and the donor and acceptor groups ... collected at the ESRF in Grenoble on the MAR Research CCD detector at 100 K, processed with DENZO and scaled and merged with SCALEPACK [26] The structure was determined using the Molecular Replacement ... concentration Data from Fig Fig FRET between TLL (and its mutants) and cis-PnA in the presence and absence of NaTDC The protein concentration was 0.8 lM and the cis-PnA concentration was varied stepwise...

Ngày tải lên: 08/03/2014, 23:20

... reassembly of membrane lymphotoxin-ab ligands (LTa1b2 and LTa2b1) [32] A LTa1b2 complex is composed of one LTa and two LTbs, and a LTa2b1 complex contains two LTas and one LTb By simply adjusting the ... GnRH-R–GFP and GnRH-R–EYFP were prepared by infecting Tn5 cells with recombinant baculoviruses Additionally, cells coexpressing GnRH-R–GFP and cytosolic EYFP, and cells coexpressing GnRH-R–EYFP and ... purified, and a double digestion with BamHI and EcoRI carried out The GnRH-R–PVL1393 vector arm was prepared by removal of GFP from the BamHI and EcoRI sites, and the prepared EYFP insert was ligated...

Ngày tải lên: 30/03/2014, 20:20

... long strand or to the 15-mer (data not shown) As expected, dyes bound to the long strand fluoresced normally and light levels decreased abruptly upon rupture as the bead carrying the DNA strand was ... output optics, including a cooled, intensified charge-coupled device (CCD) camera, a conventional black -and- white CCD camera, and two silicon avalanche photodiodes (SAPDs), are shown to the left ... simultaneous singlemolecule fluorescence and optical trapping The choices of dyes and excitation wavelengths were optimized to minimize two-photon excitation and achieve acceptably long fluorescence...

Ngày tải lên: 06/08/2014, 18:20

... between donor and acceptor dyes, will therefore unveil the status of physical union and separation of complementary strands during renaturation and strand exchange, respectively, and thereby assesses ... renaturation (A) and strand exchange (B) reactions mediated by OsDmc1 protein Phi-C and Phi-W represent rhodamine and fluorescein carrying strands, respectively, at their 3¢ and 5¢ ends The assays ... cereveciae, which had sequence similarities with recA and RAD51 ScDMC1 mutants showed defective reciprocal recombination, accumulation of double strand breaks and failure to form synaptonemal complexes,...

Ngày tải lên: 19/02/2014, 07:20

... Anti-gp120 Ig and anti-FLAG Ig identified bands corresponding to proteins with a molecular mass equivalent to  43 kDa and 85 kDa for D2L and  39 kDa and 80 kDa for D2S (Fig 1) No bands were detected ... expression of HIV- and FLAG-tagged D2L and D2S receptors in Sf9 cells To achieve this, mAbs directed against gp120 (clone 11/4C) and the FLAG sequence were used, and Fig shows the band pattern visualized ... frugiperda (Sf9) and HEK293 cells Our data show that both D2L and D2S form constitutive homooligomers in living cells that can be detected by FRET and constitutive hetero-oligomers that can be detected...

Ngày tải lên: 21/02/2014, 00:20

... 2B and Table 2) We expressed donor and acceptor proteins in the pairs pVHL30-cerulean and elongin C-citrine, cerulean-pVHL30 and citrine-elongin C, and cerulean-pVHL30 and elongin C-citrine, and ... combinations: cerulean-elongin B and citrineelongin C; cerulean-elongin B and elongin C-citrine; elongin B-cerulean and elongin C-citrine; and elongin B-cerulean and citrine-elongin C Transfected ... could be detected in the combination of ceruleanelongin B and elongin C-citrine, as shown in Fig 1D and Table FRET between elongin B-cerulean and elongin C-citrine was weak (Fig 1E), and FRET...

Ngày tải lên: 16/03/2014, 05:20

... GFP/8C1 and 8NTm1 were as described in [20] CFP/AC8 and YFP/AC8 were obtained by switching the GFP of GFP/AC8 into CFP and YFP between the restriction enzyme sites Nhe1 and BglII, from pECFP and ... cluster (Tm2) and second cytoplasmic loop (C2) (see later) The C1 and C2 regions are further subdivided into the highly conserved catalytic C1a and C2a regions and the less conserved C1b and C2b domains ... measured for after adding, 20 lM forskolin and 10 lM prostaglandin E1 for transfections involving AC5 and AC6; 20 lM forskolin, 10 lM prostaglandin E1 and mM Ca2+ for transfections involving AC8...

Ngày tải lên: 23/03/2014, 21:21

... double helix structure Each DNA strand has its own polarity One strand starts with 5’ and ends with 3’ while the other strand runs towards the opposite direction As a result, DNA double strands have ... 1.4 The structures of the nucleosome and chromatin fiber: (a) Structure of a nucleosome core 146 bp of double-stranded DNA coated on a histone protein disk to form a ~11 nm bead-on-string structure ... strands and the intra-strand stacking interaction (the repel force between two neighboring negatively charged base pairs) between the adjacent base pairs forces the two strands to intertwine and...

Ngày tải lên: 12/10/2015, 17:34

... between cerulean–GAPDH and citrine–GAPDH monomers constituting hybrid tetramers (A) Structure of fluorescent chimeric GAPDH proteins and tetrameric structure of GAPDH The structure of chimeric ... SGLRSRAQASNS The tetrameric structure of GAPDH is schematically shown on the right The distances between the Nterminal amino acids of subunits O and P, subunits O and Q and subunits O and R are shown (B–D) ... in Figs 1A and S1A, transiently expressed GAPDH–citrine (chimeric protein with N-terminal GAPDH and C-terminal citrine) and citrine–GAPDH (chimeric protein with N-terminal citrine and C-terminal...

Ngày tải lên: 16/02/2014, 09:20

... and lg of various plasmids (pSI, CaR-pSI, CaRD1036-V5/His, CaRD886-V5/His, m1-pCD, H1pCDNA3 and GABAB2-pCDNA3) and assayed as described in Experimental Procedures of WT CaR, CaRD1036-V5/His and ... protein (EGFP) and GFP2, which are the F64L/S65T and F64L mutants of GFP, respectively [45] CaRD1036-EGFP and CaRD1036-Rluc were created by subcloning of the Fig The Rluc-, GFP2- and EGFP-tagged ... Cellular expression of the GFP- and Rluc-tagged CaRs To estimate the overall expression levels of Rluc- and GFP2-tagged CaRs and the control constructs in the cells and to compare the overall cellular...

Ngày tải lên: 08/03/2014, 09:20

... particularly PLCb and -d, with PLCb activated by Gq in a calcium-dependent manner [28] and purified PLCd activated by calcium alone [26] Thus, although PC12 cells express significant amounts of PLCd, an increase ... Photonics) equipped with a dichroic mirror (510LP) and the barrier filters 480DF30 and 535DF25 for YFP–PHD and CFP–PHD, respectively Calcium and Ins(1,4,5)P3 increases were expressed as changes ... calcium and Ins(1,4,5)P3 levels in PC12h cells Cellular calcium (upper traces) and Ins(1,4,5)P3 (lower traces) were measured in PC12h cells containing Fura ⁄ AM or expressing CFP–PHD and YFP–PHD,...

Ngày tải lên: 30/03/2014, 03:20

... kinematics and dynamics of machines and mechanisms can be taught I have taught kinematics and dynamics of machines and mechanisms for many years and have always felt that concepts and numerical ... Fundamentals of Kinematics and Dynamics of Machines and Mechanisms PROBLEMS What is the difference between a linkage and a mechanism? What is the difference between a mechanism and a structure? Assume ... and sin α in Equations 2.21 and 2.22  α∗   π – α∗ α =   π + α∗   2π – α∗ if cos α > and sin α > if cos α < and sin α > if cos α < and sin α < (2.24) if cos α > and sin α < Note, that in...

Ngày tải lên: 08/04/2014, 11:35

Ngày tải lên: 06/10/2014, 15:21

... 85 7.2 Model and methodology 87 7.3 Results and discussion 89 7.3.1 Random trap and barrier model 97 7.4 Summary 101 Conclusions and future work ... NEMS, and for guiding self-organized patterns and structures Recently, a graphene Moiré pattern was employed to trap and construct arrays of C60 molecules for homoepitaxy of graphene nanostructures ... help and valuable discussions and comments I would like to give my thanks to the staffs in IHPC (especially Dr Palla Murali, Dr Liu Ping, Dr Bharathi M Srinivasan and, Dr R Hariharaputran, and...

Ngày tải lên: 08/09/2015, 17:49

... 5, 30 and 60 nM of icariside I and 71 icariside II, respectively and internal standard in pooled rat sera, were placed at the beginning and end of each assay for determination of accuracy and imprecision ... the conversion and inference from estrogenic products due to the aromatization of 4-androstene-3, 17-dione and testosterone that may be present in the sample Calibration standards and QC samples ... 21 and 65 years, body mass index between 19 and 29 kg/m2, and normal hepatic and renal laboratory tests Subjects who were taking dietary supplements in the previous months, current smokers, and...

Ngày tải lên: 10/09/2015, 15:48

... estradiol and (B) estrone (E1) concentrations via LC-MS/MS; and (C) ERα, (D) ERβ, (E) breast cancer cell (MCF-7) and (F) androgen receptor (AR) bioactivities Data points were the means±SEM and expressed ... with R2 of > 0.994 and > 0.998 for icaritin and desmethylicaritin respectively The LOD and LOQ for icaritin were estimated to be 11 pM and 33 pM respectively, with σ=0.0096 nM, and S=2.8290 Corresponding ... desmethylicaritin were 23 and 70 pM respectively, with σ= 0.0134 nM, and S=1.9082 The chromatogram of blank serum and serum spiked with 50 pM of icaritin and desmethylicaritin standards, which is relatively...

Ngày tải lên: 10/09/2015, 15:54

... determined to be 8.1% and 16% respectively Detection limits for estradiol in ERα and ERβ bioassays were 8.4 and 13.1 pM, respectively This set of assays is simple and rapid to perform and offers highthroughput, ... ability of ERα and ERβ stable cell lines and MCF-7 breast cancer cells to detect estrogenic agonistic and antagonistic activities of mixtures was studied ERα and ERβ stable cells; and MCF-7 breast ... pubescens decoction Serum samples were obtained and assayed ex-vivo for levels of estrone and estradiol by tandem mass spectrometry, for ERα and ERβ bioactivity and MCF-7 breast cancer cell proliferative...

Ngày tải lên: 10/09/2015, 15:54

... pubescens decoction Serum samples were obtained and assayed ex-vivo for levels of estrone and estradiol by tandem mass spectrometry, for ERα and ERβ bioactivity and MCF-7 breast cancer cell proliferative ... biochemical markers, serum estrogen and prenylflavonoid levels and bioactivity, and uterine wet weight will be monitored to assess whether the administration of standardized Epimedium extract could ... and sulfatase Non-linear pharmacokinetics and prolonged effects for up to 72 h following administration of a single dose of Epimedium extract were also observed Coupled processes of enteric and...

Ngày tải lên: 10/09/2015, 15:54

... 27008–27016 Mak, P., Cruz, F.D and Chen, S 1999 A yeast screen system for aromatase inhibitors and ligands for androgen receptor: yeast cells transformed with aromatase and androgen receptor Environ ... actions and functional roles in health and disease In Nuclear Receptors: Current Concepts and Future Challenges (eds Bunce, C & Campbell, M.J.) 91–141 (Springer, The Netherlands) Nowell, S and Falany, ... Barnard, S and Chee, M.S 2004 Decoding randomly ordered DNA arrays Genome Res 14(5):870-7 Hamers, T., Kamstra, J.H., Sonneveld, E., Murk, A.J., Kester, M.H., Andersson, P.L., Legler, J and Brouwer,...

Ngày tải lên: 10/09/2015, 15:54