... data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method Methods 2001, 25:402-408 Pfaffl MW: A new mathematical model for relative quantification in real-time RT ... Stevens J, Livak KJ, Williams PM: Real time quantitative PCR Genome Res 1996, 6:986-994 Nolan T, Hands RE, Bustin SA: Quantification of mRNA using real-time RT -PCR Nat Protocols 2006, ... Issue 2, Article R19 Method qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data Jan Hellemans, Geert Mortier, Anne De Paepe,
Ngày tải lên: 14/08/2014, 17:22
... Automatic TouchDown one-step real-time gene synthesis Automatic TouchDown (ATD) one-step process was optimized using real-time PCR conducted with Roche’s LightCycler 1.5 real-time thermal cycling machine ... the real-time fluorescence signals to study the effects of the oligonucleotide concentration, stringency of annealing temperature, duration of PCR extension (which would reflect the half-time ... temperature, and Tm uniformity The real-time gene synthesis with fluorescence monitoring described herein would help by providing instant feedback, terminating the process in time as it reached the plateau
Ngày tải lên: 14/09/2015, 08:48
REAL TIME BEST VIEW SELECTION IN CYBER PHYSICAL ENVIRONMENTS
... Results -53 4.1 The user interface -54 4.2 Best view acquisition of single object -54 4.3 Best view acquisition of human -55 4.4 Extensions for web-based real time applications -56 4.5 Quality of Experience ... to obtain the best view upon the user’s selection Since the Viewpoint Saliency measure is purely image-based, 3D model reconstruction is not required And then we map the real time best view selection ... make adaptive responses Figure 1.1 Illustration of the best view selection problem Trang 16To make best view acquisition feasible for real time streaming applications such as video conferencing
Ngày tải lên: 16/10/2015, 15:38
Lecture Building reliable component-based systems - Chapter 14: Testing reusable software components in safety-critical real-time systems
... Chapter 14 Testing Reusable Software Components in SafetyCritical RealTime Systems Introduction How dynamic verification of real-time software relates to component reuse in safety-critical real-time systems ... Chapter 14 Testing Reusable Software Components in SafetyCritical RealTime Systems Chapter 14 Testing Reusable Software Components in Safety-Critical Real-Time Systems Trang 2Building Reliable Componentbased Systems ... Chapter 14 Testing Reusable Software Components in SafetyCritical RealTime Systems Trang 22Building Reliable Componentbased Systems Chapter 14 Testing Reusable Software Components in SafetyCritical RealTime Systems Response Time R(c) C(c) Response time
Ngày tải lên: 15/05/2020, 22:08
Real time software engineering (CÔNG NGHỆ PHẦN mềm SLIDE)
... 21– Real-time Software EngineeringTrang 2Topics covered Embedded system design Architectural patterns for real-time software Timing analysis Real-time operating systems Trang 3Embedded software ... actions in real-time pump control transitions to the Delivering state After the fuel delivery is complete and the hose replaced Trang 23Real-time programming Programming languages for real-time ... personal software systems For non-real-time systems, correctness can be defined by specifying how system inputs map to corresponding outputs that should be produced by the system In a real-time
Ngày tải lên: 29/03/2021, 07:59
a component based process with separation of concerns for the development of embedded real time software systems
... generated using the software model as input The former implements concurrency and real-time concerns; the latter the interaction between components In our approach, the full real-time and communi-cation ... components All other concerns (concurrency and real-time, dependability, deployment) are addressed in separate development steps Figure 3 depicts concurrency, real-time and deployment, which are directly ... generation of correct-by-construction code artefacts 2.2 Software entities The real-time architecture of our component model features three distinct software entities: the component, the container and
Ngày tải lên: 01/11/2022, 08:29
Real-Time Digital Signal Processing - Chapter 1: Introduction to Real-Time Digital Signal Processing
... quantization and arithmeticerrors 1.1 Basic Elements of Real-Time DSP Systems There are two types of DSP applications ± non-real-time and real time Non-real-timesignal processing involves manipulating signals ... result is not a function of real time Real-time signal processing places stringent demands on DSP hardware and software design to complete predefined tasks within a certaintime frame This chapter ... andmaintenance Software maintenance is a significant part of the cost of a softwaresystem Maintenance includes enhancing the software, fixing errors identified as thesoftware is used, and modifying the software
Ngày tải lên: 17/10/2013, 14:15
Real-Time Digital Signal Processing - Chapter 2: Introduction to TMS320C55x Digital Signal Processor
... registers (MMRs) 2.3 Software Development Tools The manufacturers of DSP processors typically provide a set of software tools for the user to develop efficient DSP software The basic software tools ... language is the most popular high-level tool for evaluating DSP algorithms and developing real-time software for practical applications The TMS320C55x C compiler translates the C source code ... data bus At the same time, the IU can decode up to six bytes of program After four program bytes are fetched, the IU places them into the 64-byte instruction buffer At the same time, the decoding
Ngày tải lên: 19/10/2013, 23:15
Real-Time Digital Signal Processing - Chapter 3: DSP Fundamentals and Implementation Considerations
... is, M x max n fjxnjg: 3:2:3 Window at time n n−L Time n−1 n−L+1 n Window at time n−1 Figure 3.7 Time windows at current time n and previous time n À 1 INTRODUCTION TO DIGITAL FILTERS 85 ... For example, a real-valued sinewave of amplitude A defined in (3.1.6) has the power P x 0:5A 2 . In most real-time applications, the power estimate of real-valued signals at time n can be expressed ... variable... implementation and testing by simulator Real-time testing in target system End Figure 3.17 Implementation procedure for real-time DSP applications x(n) Ideal system Practical
Ngày tải lên: 19/10/2013, 23:15
Real-Time Digital Signal Processing - Chapter 5: Design and Implementation of FIR Filters
... This is impossible for any real physical filter Linear, time-invariant filters are characterized by magnitude response, phaseresponse, stability, rise time, settling time, and overshoot Magnitude ... values Such a filter is `non-realizable' in realtime, but is easy to implement for the stored data The non-causal filter has the I/Oequation y n XL2l L 1 interpola-5.2.4 Realization of FIR Filters ... resulting filter is non-causal, thus is non-realizable for real-time applications A finite-duration impulse response fh0 ng of length L 2M 1 that is the bestapproximation (minimum mean-square
Ngày tải lên: 23/10/2013, 22:15
Real-time RT-PCR
... help you to familiarize yourself with the jargon of real-time RT-PCR. 9.2 Basic principles of real-time RT-PCR The basic principle of real-time RT-PCR is much like conventional RT-PCR: cDNA is ... real-time RT-PCR Since the advent of real-time RT-PCR the number of applications has grown exponentially It is outside the scope of this book to describe every application of real-time ... mRNA targets of Real-time RT-PCR 223 Table 9.2 Real-time thermal cyclers PCR system Company Excitation specifications Detection specification Applied Biosystems 7300 Real-Time PCR System
Ngày tải lên: 25/10/2013, 22:20
Real-Time Digital Signal Processing - Chapter 6: Design and Implementation of IIR Filters
... find and draw the following realizations: (a) direct-form II, (b) cascade realization based on direct-form II realization of each section, and (c) parallel direct-form II realization 16 The difference ... assembly code SOFTWARE DEVELOPMENTS AND EXPERIMENTS USING THE TMS320C55X 293 Run-time support library functions The TMS320C55x C compiler has many built-in C functions in its run-time support ... very inefficient for real-time applications This is because the floating-point implementation needs the floating-point math library functions that use fixed-point hardware to realize floating-point
Ngày tải lên: 28/10/2013, 05:15
Real-Time Digital Signal Processing - Chapter 7: Fast Fourier Transform and Its Applications
... frequency analysis on a discrete-time signal, we converted the time-domain sequence into the frequency-domain representation using the z-transform, the discrete-time Fourier transform (DTFT), or ... ÀImX N À k, Thus the DFT of a real sequence produces symmetric real frequency components and anti-symmetric imaginary frequency components about X(M) The real part of the DFT output is an ... scaling method increases software complexity and may require longer execution time 7.5 Experiments Using the TMS320C55x In this section, we implement the decimation-in-time FFT algorithm using
Ngày tải lên: 28/10/2013, 05:15
Real-Time Digital Signal Processing - Chapter 8: Adaptive Filtering
... a time function x n x n, A with every possible outcome A of an experiment Each time function is called a realization of the random process or a random signal The ensemble of all these time ... consider a particular sample function, we have a time function and the statistics we obtain are temporal For example, Ex n, Ai is the time average If the time average is equal to the ensemble average, ... if all its statistics can be determined from a single realization of the process, provided that the realization is long enough Therefore time averages are equal to ensemble averages when the
Ngày tải lên: 06/11/2013, 18:15
Tài liệu DS12C887_ Real Time Clock pdf
... WR , or RD Chip Select Hold Time Read Data Hold Time Write Data Hold Time Mux’ed Address Valid Time to ALE Fall Mused Address Hold Time to ALE Fall Delay Time DS/E to AS/ALE Rise Pulse ... after the data mode bit has been written to allow the real time clock to update the time and calendar bytes. Once initialized, the real time clock makes all updates in the selected mode. The ... DS12C887 REAL TIME CLOCK ADDRESS MAP Figure 2 DS12C887 6 of 19 TIME, CALENDAR AND ALARM LOCATIONS The time and calendar information is obtained by reading the appropriate memory bytes. The time,
Ngày tải lên: 12/12/2013, 09:15
Tài liệu Equal Access Means Equal Opportunity for All: Creating a Real-Time Statewide Advanced Placement Consortium February, 2003 ppt
... their AP Consortium courses in their respective schools in a “real time” environment. AP Consortium teachers may supplement this “real time” instruction through use of web pages created and supported ... advanced placement courses for Wisconsin’s rural, low income, and minority students by using “real time” classroom instruction delivered over Wisconsin’s fiber optic BadgerNet. Trang 4Project ... Trang 1Equal Access Means Equal Opportunity for All: Creating a Real-Time Statewide Advanced Placement Consortium February, 2003 Trang 2AP Consortium PurposeIncrease
Ngày tải lên: 15/01/2014, 15:59
Tài liệu Real-Time Digital Signal Processing - Appendix B: Introduction of MATLAB for DSP Applications docx
... xn); title(`Time-domain signal x(n)'); xlabel(`Time index'); ylabel(`Amplitude'); where title gives the plot with the title `Time-domain signal x(n)', xlabel labels the x-axis with `Time index' ... There is no need to declare variables as integer, real (float or double in C), or complex because MATLAB automatically sets the variables to be real with double precision The output of every command ... xlabel(`Time index'); ylabel(`Amplitude'); The waveform of the generated sinewave is shown in Figure B.1 In Figure B.1, a trivial integer index n is used for the x-axis instead of an actual time
Ngày tải lên: 25/01/2014, 19:20
Tài liệu Real-Time Digital Signal Processing - Appendix C: Introduction of C Programming for DSP Applications ppt
... this book is conformed to the ANSI C standard (American National Standard Institute C Standard) Real-Time Digital Signal Processing Sen M Kuo, Bob H Lee Copyright # 2001 John Wiley & Sons Ltd ... Detailed comments are very important in maintaining complicated DSP software for new readers, even for the original programmers after time has passed The preprocessor is the first pass of the C compiler ... This reusability is a very important issue in the development of large software systems because it greatly reduces development time 4 The use of functions frequently reduces the overall length of
Ngày tải lên: 25/01/2014, 19:20
Đánh giá sự biểu biểu hiện gen mã hóa pheromone lai alpha (MF(ALPHA)1) ở các chủng pichia pastoris tái tổ hợp sinh amylase và interleukin 2 bằng kỹ thuật real time PCR
... bào nấm men; tối ưu điều kiện cho phản ứng Realtime PCR; kết Real-time PCR với gene nội chuẩn IPP1; real-time PCR biểu gene MF(ALPHA)1; so sánh kết Real-time PCR Microarray Keywords: Sinh học thực ... kiện để sử dụng cho Real-time PCR 3.3 Tối ƣu điều kiện cho phản ứng Real-time PCR 3.3.1 Tối ƣu điều kiện Real-time PCR với gene chuẩn IPP1 Tối ưu nồng độ mRNA phản ứng Real-time PCR Tiến hành ... ứng PCR, gọi “Real-time” Kỹ thuật Real-time PCR đặc trưng giới hạn định lượng rộng, độ nhạy cảm cao đủ để phát phân tử khả lặp lại cao (độ lệch chuẩn nhỏ 0,2 chu kỳ) Kỹ thuật Real-time PCR đặc
Ngày tải lên: 10/02/2014, 15:15
Xác định kiểu gen virut viêm gan c trong huyết thanh bệnh nhân viêm gan c bằng kỹ thuật sinh học phân tử real time – PCR
... lượng phương pháp Real-time Kĩ thuật tách chiết ARN Kĩ thuật tổng hợp cDNA với mồi ngẫu nhiên Kỹ thuật Real - time PCR sử dụng Taqman Probe phát đinh lượng HCV-RNA Kĩ thuật Real-time PCR sử dụng ... quang vượt tín hiệu nền, mẫu chứng âm (được thiết kế với thành phần tương tự cho phản ứng real-time PCR real-time RT PCR) có tín hiệu huỳnh quang thấp tín hiệu Tuy nhiên 228 bệnh phẩm xác định genotype ... nhầm lẫn type type 30 mẫu định type phương pháp Real-time PCR với phương pháp giải trình tự gen 11/27 mẫu 40,74% Như định type phương pháp Real-time PCR xét mặt kinh tế phù hợp với đa số điều
Ngày tải lên: 10/02/2014, 20:55