Pereira CONTENTS 6.1 Introduction 6.2 Active Extracts from Wheat Straw 6.2.1 Phenolic Compounds 6.2.2 Fatty Acid Derivatives 6.2.3 Triterpenoids 6.2.3.1 HPLC-MS of Ketosteroids 6.2.3.2 S
Trang 1Triterpenoids and Other Potentially Active
Compounds from Wheat Straw: Isolation,
Identification, and Synthesis
Elvira Maria M S M Gaspar, H J Chaves das Neves, and
M Manuela A Pereira
CONTENTS
6.1 Introduction
6.2 Active Extracts from Wheat Straw
6.2.1 Phenolic Compounds
6.2.2 Fatty Acid Derivatives
6.2.3 Triterpenoids
6.2.3.1 HPLC-MS of Ketosteroids
6.2.3.2 Synthesis
6.3 Conclusions
References
Straw consists of the above-ground fractions (normally cut at a height of around 20 cm) of cereal plants after removal of the grain.1 Depending on the harvesting system, part of the cut straw is left in the field together with the stubble The straw length and diameter vary greatly and, consequently, also the biomass yield both within and between species The bio-mass production and its chemical composition are important parameters since straw and other fibrous by-products from cereals that are available in the world amount to approxi-mately 3 trillion tonnes per year.2 Part of the straw is utilized for feed,3 paper,4 and fuel,5 but a major part of the straw is discarded as a waste product In some regions of the world straw is used in mulch-tillage in no-till cropping systems,6,7 a common agricultural practice credited with a number of ecological advantages such as reduction of soil compaction, good erosion control, better water retention, and conservation of organic matter In many instances, inhibitory effects on germination and growth of other plant species were observed The direct or indirect, harmful or beneficial effects of plants on other plants of the same
or of different species by means of chemicals released from living, or decaying, plant mate-rial has been broadly defined as allelopathy.8 The presence of chemical signals in ecosys-tems has been attributed to numerous types of secondary metabolites9 which are able to
Trang 2induce physiological and morphological changes in plants: inhibition of respiration, ger-mination and growth, perturbation of nutrient uptake, chlorosis, and death
Although allelopathic effects of wheat straw upon some weeds have been known for some time, few compounds have been associated with these effects However, due to their potential application in the understanding and developing of natural herbicides and to their economical and ecological importance in agriculture, there is a need for research on the active principles present in straw to properly understand the allelopathic phenomenon and clearly establish a cause–effect relationship
6.2 Active Extracts from Wheat Straw
A systematic extractive workup of wheat straw yielded two major fractions that showed allelopathic activity in lettuce bioassays: a weak acidic fraction, mainly composed of phe-nolic compounds and a “neutral” fraction which was shown to be composed of triterpe-noid structures and fatty acid derivatives
In order to establish the composition of phenolics from cereal straws, packed column GC and GC-MS have been used in the analysis of ethylated phenolic compounds and their mass spectra discussed, but only a few compounds have been identified.10 Today HPLC is the most commonly used method for analyses of phenolics of plant origin.11,12 However, resolution is highly dependent upon eluant composition and the analytical conditions must be carefully optimized according to expected phenolic structures.13 Some authors have studied the application of HPLC with electrochemical detection to chromatographic assays of phenolic compounds from wheat straw.14 Only six compounds (p-hydroxyben-zoic acid, vanillic acid, vanillin, syringic acid, p-coumaric acid, and ferulic acid) were
iden-tified as free phenols
In the author’s laboratory, the high resolution, speed of analysis, and sensitivity of cap-illary gas chromatography, coupled with mass spectrometry (HRGC-MS) and Fourier transform infrared spectrometry (HRGC-MS-FTIR), was used to assess the composition of the phenolics extracted from mature wheat straw.15 In the case of some phenolic com-pounds of low molecular weight, distinction between isomers is particularly difficult by HRGC-MS Also, phenolic compounds frequently appear partially methylated in nature Furthermore, the position of the nonalkylated OH groups cannot be assessed after perme-thylation In order to distinguish between free and methylated OH, the straw extract was ethylated with diazoethane in methanol and the resulting mixture analyzed by HRGC Under these conditions, salicylic, gentisic, and β-resorcylic acids are only partially ethy-lated as is shown by the presence of a free OH band (3547 cm–1) in the corresponding
HRGC-FTIR spectra The ortho OH groups are not derivativized The fact that the mass
spectrum of syringic acid showed a molecular ion at m/z 254, corresponding to the ethy-lation of the hindered OH group in position 4, seemed to suggest that hydrogen bonding effects, rather than steric effects, are responsible for the incomplete ethylations Ethylation with diazoethane is, therefore, a useful method for the distinction of isomeric phenolic acids by GC-MS The ethyl derivative of 3,4-dihydroxybenzoic acid (protocatechuic acid) showed a molecular ion at m/z 238, but the derivatives 2,4 (β-resorcylic acid) and 2,5 (gen-tisic acid) yielded a molecular ion at m/z 210 The same distinction can be made between
salicylic acid and p-hydroxybenzoic acid.
Trang 3Individual organic compounds possess characteristic infrared spectra with unique absorption patterns in the “fingerprint” region (below 1600 cm–1) The FTIR, with its facility for electronic retrieval and spectral comparison, offers a powerful tool for distinction between isomers For instance, gentisic and β-resorcylic acids can be differentiated by means
of the corresponding FTIR spectra Combination of HRGC-MS results and HRGC-FTIR spectral data led us to the identification of the most significant components in the extract
Ferulic, protocatechuic, gentisic, p-hydroxybenzoic, trans-p-coumaric, and azelaic acids
were obtained on a preparative scale from raw extracts by droplet countercurrent chroma-tography (DCCC).15 This technique has many advantages in preparative chromatography Sample losses due to compound adsorption are not observable, as all the process takes place in the liquid phase.16 This is particularly important in the case of phenolic com-pounds Its main drawbacks are the large amounts of solvents used and the length of time Raw extracts could be directly used for DCCC The isolated components could be recov-ered in high purity after solvent evaporation Their identity was confirmed by HNMR, IR,
UV, and MS Table 6.1 shows the identified components The majority of these compounds
TABLE 6.1
Phenolic Acids Identified in the Acidic Extract of Mature Wheat Straw
Compound
m/z (ITD) (Ethyl Derivatives)
cm –1 (FTIR) (Ethyl Derivatives)
Benzoic acid 150 (M +), 149, 122, 106, 105,
77
3034, 2982, 2984, 1740, 1448, 1369, 1270, 1107, 709 Succinic acid 174 (M +), 147, 130, 129, 101 2988, 2944, 1755, 1373, 1345, 1270, 1210, 1180, 1037, 960 Fumaric acid 172 (M +), 143, 142, 114, 69 2988, 2943, 2913, 1743, 1647, 1471, 1401, 1370, 1296, 1259,
1227, 1154, 1103, 1041, 983
m-Toluic acid 164 (M +), 163, 120, 119, 91 3030, 2940, 1720, 1590, 1450, 1390, 1280, 1210, 1110, 1090,
790, 750, 690 Salicylic acid a 166 (M +), 165, 120, 93, 92 3547, 3261, 2985, 1690, 1615, 1475, 1400, 1374, 1310, 1253,
1208, 1159, 1133, 1084, 755, 686 Maleic acid 172 (M +), 127, 117, 99, 84 2990, 1730, 1640, 1410, 1380, 1290, 1210, 1160, 1020, 860
p-Hydroxybenzoic acid 194 (M +), 166, 149, 138, 121,
93, 65
2958, 2942, 2924, 1745, 1600, 1483, 1452, 1375, 1291, 1242,
1126, 1080, 1049, 752, 681 Gentisic acid a 210 (M +), 164, 136, 135, 111 3547, 2988, 1736, 1605, 1509, 1269, 1247, 1168, 1104 Vanillic acid 224 (M +), 196, 168, 151, 123,
97
3090, 2972, 1736, 1604, 1512, 1432, 1373, 1283, 1216, 1101,
1032, 767 β-Resorcylic acid a 210 (M +), 181, 136 3547, 2958, 2942, 2924, 1745, 1600, 1483, 1452, 1375, 1291,
1242, 1126, 1080, 1049, 752, 681 Protocatechuic acid 238 (M + ), 210, 182, 165, 154,
137
2984, 2943, 1735, 1600, 1505, 1477, 1413, 1369, 1284, 1214,
1180, 1127, 1101, 1032, 761 Azelaic acid 244 (M + ), 199, 152, 135, 125,
111, 83, 69, 55
2983, 2940, 2868, 1752, 1372, 1304, 1172, 1161, 1114, 1098,
1079, 1047 α,β-Dihydroferulic acid 252 (M +), 224, 151, 150, 137 2982, 1735, 1604, 1425, 1372, 1255, 1216, 1186, 1104, 975
trans-p-Coumaric acid 220 (M +), 175, 164, 148, 147 2988, 1736, 1605, 1509, 1269, 1274, 1168, 1104
Syringic acid 254 (M+ ), 226, 211, 198, 181 2984, 2946, 1735, 1584, 1492, 1468, 1413, 1369, 1330, 1215,
1184, 1111, 1037
cis-Ferulic acid 250 (M+ ), 222, 194, 177 2985, 1733, 1637, 1607, 1508, 1306, 1259, 1210, 1163, 1045,
1037, 672
3,4-Dihydroxyphenyl-propanoic acid
266 (M+ ), 238, 224, 210, 165, 147
3647, 3574, 3081, 3022, 1736, 1604, 1404, 1251, 1164, 1118,
905, 825 b
trans-Ferulic acid 250 (M+ ), 236, 205, 136, 121 3008, 2987, 2965, 2948, 1735, 1697, 1639, 1609, 1466, 1425,
1372, 1323, 1255, 1216, 1186, 1167, 1104, 975 1-Naphthoic acid 171 (M +-29), 127, 99 3574, 3055, 1750, 1550, 1504, 1344, 1164, 1105, 938, 772,
712 b
a Partial ethylation was observed.
b Spectrum corresponding to the underivatized acid.
Trang 4show significant biological activity They are the most cited allelopathic agents possessing very significant allelopathic effects towards weeds.17
6.2.2 Fatty Acid Derivatives
Malic, malonic, citric, acetic, tartaric, fumaric, propionic, butyric, isobutyric, pentanoic, iso-pentanoic, nonanoic, decanoic, lauric (12:0), myristic (14:0), palmitic (16:0), stearic (18:0), oleic (18:1; 9c), linoleic (18:2; 9c,12c), linolenic (18:3; 9c,12c,15c), arachidonic (20:0),
11,14-eicosadienoic, hen11,14-eicosadienoic, behenic (22:0) acids, and
(Z,Z)-9,12-8-hydroxyoctadecadi-enoic are the most frequently reported carboxylic acids present in active allelopathic extracts, or as biologically active compounds in the literature.9,18-20 McCracken et al.21 reported that the unsaturated compounds were the most inhibitory; their toxicity increas-ing with the number of unsaturated bonds
Classical methods for isolating, separating, and identifying fatty acids and derivatives comprise distillation (steam distillation, fractional reduced pressure, and molecular distil-lation), recrystallization, countercurrent distribution, and the chromatographic methods: column partition chromatography (CC), paper chromatography (PC), and thin-layer chro-matography (TLC).22 Nowadays, because of its high resolution power, GC is the most pop-ular method.23,24 Usually acids are separated as methyl esters derivatives The more volatile ones (C3-C9) are frequently derivatized as butyl, decyl, and other ester derivatives of higher molecular weight There is a growing interest in HPLC,25 especially when other functionalities rather than unsaturated bonds are present
The classical structure methods comprise the spectroscopic ones: UV,22 to determine conju-gated bonds; IR,22 to recognize special funcionalities and also trans double bonds; nuclear
magnetic resonance spectroscopy (NMR),26 to assign structures; and also mass spectrometry GC-MS is the most commonly used method for separation and identification of medium and long chain carboxylic acid derivatives The mass spectra afford direct information regarding chain length, degree of unsaturation, position of branching, and the nature and position of functional groups Yet, double bond positions cannot be unequivocally assigned due to the frequent migration of double bonds on fragmentation The use of negative ion chemical ionization (CI–) is ineffective in that regard.27,28 A more sophisticated method using collisional activated decomposition (CAD) of negative ions (M-H)– was successfully used to determine the position of double bonds in unsaturated compounds.27 A tandem tri-ple mass spectrometer analyzer (MS/MS) must be used, but it is not available in the major-ity of laboratories The best solution has been the use of double bond derivatives which can
be analyzed in current electron impact mass spectrometers
The neutral fraction, resulting from a systematic acid-base solvent extraction procedure, was shown to be composed of fatty acid methyl esters and triterpenoids Carboxylic methyl esters were identified by GC analysis, by comparison with retention times of stan-dards, and by analysis of their mass spectra obtained by HRGC-MS.29 The differences in chain length, branch position, degree of unsaturation, position, and configuration of dou-ble bonds required careful GC and MS analysis with capillary columns Generally,
separa-tion and identificasepara-tion of cis-trans mixtures require specialized chromatographic
conditions, including the use of very long capillary columns or more polar stationary phases However, these phases have lower temperature stability and the mass spectra of positional and geometrical isomers are difficult to differentiate The dimethyl disulfide derivatives (DMDS)30-34 offered us an excellent method for the analysis of the monounsat-urated fatty acid esters present This is a single-step derivatization procedure The mass spectra of DMDS adducts showed molecular ions (M)+ and two main ions (A+ and B+) derived from the cleavage of carbon–carbon bond where the double bond was originally
Trang 5located and also an important fragment C+ derived from B+ via loss of methanol (B+-32).
Alkylthiolation of Z- and E-isomers as a specific anti-addition led, respectively, to the threo and erythro adducts which were well separated by gas chromatography in a nonpolar
sta-tionary phase column (OV-101) This allowed a stereochemical identification Table 6.2 lists the fatty acid methyl esters and their mass spectra identified in the neutral fraction of wheat straw The branch position was achieved by comparison of mass spectra with the mass spectra of linear compounds with similar carbon number The DMDS derivative is not effective for double bonds located at C2 and with polyunsaturated acids.30 The reaction with deuterodiimide (N2D2) is a possible derivative for polyunsaturated locations.28 Some of the identified acids were described as allelopathic or constituting a fraction hav-ing allelopathic activity This is in good agreement with the activity demonstrated by the neutral fraction of wheat straw Some authors35 believe that the biosynthetic evolution of carboxylic acids of plant origin is strictly related to the biochemical necessity of their com-ponents having low melting points and this is the reason why plants have developed a great variety of unusual carboxylic acids, in contrast with animals Perhaps this also explains why in a total of 21 identified esters, 7 are unsaturated and 4 are branched These
structural features, double bonds, and especially cis isomers and branches, lead to a
decrease in the melting point
6.2.3 Triterpenoids
Triterpenoids constitute a large group of compounds with a broad range of physical prop-erties and biological activities with their nomenclature being well described.36 The triterpe-noids present in wheat straw are cycloartane and cholestane derivatives, both tetracyclic derived structures
TABLE 6.2
Methyl Fatty Esters Identified in a Neutral Fraction of Mature Wheat Straw
Decanoate 214 (M +), 188, 173, 145, 116, 99, 87, 74 (100%)
Azelate 220 (M +), 206, 190, 178, 161, 146, 145, 131, 119, 105, 57
Syringic aldehyde 196 (M +), 181, 153, 137, 111, 83
Tetradecanoate 242 (M +), 199, 186, 157, 143, 129, 100, 87, 74, 55
10-Methylpentadecanoate 256 (M +), 228, 213, 199, 185, 157, 143, 129, 115, 101, 87, 74, 55
Pentadecanoate 256 (M +), 213, 199, 143, 129, 101, 87, 74, 55
12-Methylpentadecanoate 285 (M +), 241, 227, 199, 171, 157, 129, 101, 87, 74, 55
Palmitate 270 (M +), 237, 171, 143, 129, 115, 101, 87, 74, 55
3-Methylpalmitate 284 (M +), 255, 241, 213, 199, 185, 171, 157, 143, 101, 87, 73, 55
Heptadecanoate 284 (M +), 255, 241, 213, 199, 185, 143, 129, 101, 87, 74, 55
Stearate 298 (M +), 255, 241, 199, 185, 143, 129, 101, 87, 74, 55
2-Methylstearate 312 (M +), 269, 255, 226, 213, 199, 171, 157, 143, 101, 88, 73
Eicosanoate 326 (M +), 283, 269, 227, 213, 171, 157, 143, 129, 88, 74
(Z)-9-Hexadecenoatea 315 (M + - 47), 267, 217, 201, 185, 169, 137, 99, 71, 61
Docosanoate 354 (M +), 311, 279, 213, 199, 168, 143, 115, 101, 87, 74
Oleate a 390 (M +), 343, 325, 295, 253, 217, 204, 185, 173, 137, 87, 69, 55
Tricosanoate 368 (M +), 325, 291, 269, 253, 213, 185, 143, 101, 87, 74, 55
(Z)-10-Nonadecenoatea 404 (M + ), 372, 339, 294, 267, 231, 213, 199, 185, 173, 167, 156, 137, 97, 55
(Z)-19-Eicosenoatea 418 (M +), 403, 373, 357, 241, 193, 139, 109, 95, 67, 61, 55
(Z)-9-Eicosenoatea 418 (M +), 403, 371, 357, 339, 325, 217, 201, 187, 155, 137, 121, 87, 71, 55
Nonadecadienoate 308 (M +), 279, 262, 220, 135, 95, 81
a Dimethyl disulfide derivatives.
Trang 6With respect to toxicity, the phytotoxic effects of triterpenoids in higher plants are relatively unknown Only two compounds, digitoxigenin and estrofantidin, were mentioned as having proven antimicrobial activity.9,37 Macias et al.38 referred allelopathic properties for some oxi-dized triterpenoids as having interesting inhibitory activities at low concentrations
There is no such thing as a universal extraction method for triterpenoids The extraction technique is usually determined by the material to be extracted and is conditioned by avail-able information for the class structure present (free, glycosidic, and/or esterified) The most commonly used solvents are chloroform: methanol mixtures, acetone, chloroform, dichloromethane, petroleum ether, and ethanol Usually a small quantity of water (2 to 7%)
is added if the material is dry because its presence increases the yield of triterpenoids.39 After extraction, the first step is usually a triterpenoid class separation Again, there are several different methods employed Solvent fractionation, precipitation with digitonin, and chromatographic separation using different techniques such as adsorption column chromatography CC (silica gel and/or alumina), reverse phase or argentation, or, in the case of a small quantity of extract, by adsorption or reverse phase thin layer chromatogra-phy TLC and/or by HPLC, are among the most common reported in the literature.40 With respect to the individual separation of tetracyclic triterpenoids, the most often used methods are adsorption CC (silica gel or alumina) or reversed phase (Sephadex LH 20), reverse phase or argentation TLC, and HPLC, with HPLC being the most popular tech-nique because it results in lower losses, produces fewer artifacts, and has a greater number
of theoretical plates (higher resolution) GC is generally used as an analytical technique to evaluate the purity of isolated compounds
The application of spectroscopic techniques NMR, MS, UV, IR, and x-ray is fundamental for final unequivocal identification.36 However, the chromatographic and spectral informa-tion accumulated during the extracinforma-tion, separainforma-tion, and isolainforma-tion process are very impor-tant, particularly if the compounds are present in very small quantities and the more sophisticated spectroscopic tools as bidimensional NMR techniques and x-ray spectros-copy, or even 13CNMR spectroscopy cannot be used In these cases, the use of coupled tech-niques such as GC-MS or HPLC-MS is of paramount importance
The isolated neutral fraction of wheat straw also is composed of triterpenoids: cholestane and cycloartane derived structures Also included are seven new natural compounds:
(24R)-14α-methyl-5α-ergostan-3-one; 14α-methyl-5α-cholestan-3-one;
cycloart-5-ene-3β,25-diol; cycloart-3β,25-diol; 4α-methylergostan-5-ene-3β-ol; stigmast-4,22-dien-6β-ol-3-one and 24-phenylethyl-cholest-3,6-distigmast-4,22-dien-6β-ol-3-one were identified; together with several known
compounds: (24R)-stigmast-4-ene-3-one; ergost-4-ene-3-one; (24R)-stigmast-4-ene-3,6-dione; stigmast-4,22-diene-3,6-(24R)-stigmast-4-ene-3,6-dione; ergost-4-ene-3,6-(24R)-stigmast-4-ene-3,6-dione; (24R)-5α-stigmast-3,6-dione; stigmast-22-ene-3,6-dione; ergost-3,6-dione; cholesterol; ergosterol; campesterol; stigmas-terol; β-sitosterol; spinasterol; stigmastanol; 7β-hydroxysitosterol; stigmast-4-ene-6β-ol-3-one; and ergost-4-ene-6β-ol-3-one.29,41 Some of them were identified for the first time in higher plants
The diethyl ether soluble material, specifically from the acetone–water extract of straw, was chromatographed on a silica gel column The four fractions eluted with diethyl ether: ketones, monohydroxysterols, tetracyclic triterpenoids, dihydroxysterols, and ketohydroxy-sterols, were further separated by alumina column chromatography The individual puri-fication was done by preparative TLC and/or recrystallization from methanol or ethanol The spectroscopic tools 1HNMR, 13CNMR, FTIR, UV, LRMS, HRMS were used to make structural assignments Figure 6.1 shows the new triterpenoid compounds identified in mature wheat straw and the most important spectroscopic data for their identifications All these compounds were present in very small quantities (1 to 2 mg), and some of them were present in “trace” amount (≤ 0.1 mg) In those cases coupled techniques HRGC-MS,
HPLC-MS, and HRFTIR were used and structures were proposed based on correlations with similar
Trang 7mass spectra, rationalizing fragmentation, and, when possible, comparing the chromato-graphic behavior with standards
The advantage of HPLC-MS over HRGC-MS is its ability to provide separation of com-pounds unsuitable for HRGC analysis, without the necessity of preparing volatile deriva-tives The limitations reside in the specifications of the available interfaces and lower
FIGURE 6.1
New triterpenoid compounds from wheat straw.
Trang 8sensitivity The particle beam (PB) separator offers the advantage of a full range mass spec-tra, although it severely limits the maximum eluent flow (1 ml min–1) and cannot be used with buffered eluents We have used the PB interface in order to fully gather 70 EI mass spectral data from liquid chromatography runs of ketosteroid rich fractions The compo-nents of this fraction could not be satisfactorily resolved by HRGC Although the chroma-tography could be drastically improved after trimethylsylilation, the GC-MS runs gave complex mass spectra that were too difficult to interpret.29
Table 6.3 shows the most important ions of PB mass spectra of ketosteroids present in wheat straw Using the PB interface, the spectra seemed to be common EI spectra which offered the advantage of being superimposable on published spectra of known structures
or rationalizing their fragmentation if they are uncommon compounds
The current interest in the activity of steroid metabolites having a C-6 oxo or hydroxyl func-tion with occurrence in low concentrafunc-tions in mature wheat straw extract, led us to establish
a direct synthesis for them for unequivocal identification There are only a few examples in literature42-44that refer to the introduction of a hydroxyl group in the C-6 position of the ste-rol skeleton More generally it is the introduction of an oxo group in position 6 of choles-terol with pyridinium dichromate (PDC),45or sodium dichromate,46or in cholestenone by sodium peroxide.47
Stigmasta-4,22-dien-6β-ol-3-one was present in residual amounts in wheat straw and its
structure was unequivocally proven by a new direct synthesis, via m-chloroperbenzoic acid
(MCPBA) oxidation of the stigmast-4,22-dien-3-one enol-ether (Figure 6.2) Theβ position
of the hydroxyl group was confirmed by synthesis of a C-6 hydroxy derivative of stig-masta-4,22-dien-3-one The treatment of the crude dienol-ether of stigmasta-4,22-dien-3-one in a dichloromethane/ethanol solution that was easily prepared by reaction with
methyl orthoformate in DMF and in the presence of a catalytic amount of p-toluenesulfonic
acid monohydrate and methanol48by slow addition of a dichloromethane solution of
m-chloroperbenzoic acid at 0°C, yielded a mixture of stigmast-4,22-dien-6β-ol-3-one and stigmast-4,22-dien-6α-ol-3-one that was easily separated by chromatography on silica gel The stigmast-4,22-dien-6β-ol-3-one with a 39% overall yield was the major product, while the 6α isomer yield was 25% (Table 6.4)
A mixture of epimers, where theβ-isomer also is the predominant one, was referred to in the literature for the attack of a peracid on the enol ether of a derivative of pregn-4-ene-3-one.49
TABLE 6.3
PB-HPLC-MS Mass Spectra of Ketosterols from Wheat Straw
(24R)-Stigmast-4-ene-3,6-dione 426 (M) + , 411, 408, 398, 385, 276, 243, 187, 162, 149, 137, 121, 109, 95, 55 (100%)
(24R)-5α-Stigmast-3,6-dione 428 (M + , 100%), 413, 399, 287, 259, 245, 231, 189, 149, 137, 121, 109, 98 14α-Methyl-5α-cholest-3-one 400 (M) + , 385, 231 (100%), 217, 123, 163, 124, 107, 95, 81, 67, 55
(24R)-14α-Methyl-5α-ergost-3-one 414 (M) + , 399, 272, 245, 231 (100%), 217, 213, 189, 163, 135, 124, 107, 95, 81, 67 Ergost-4-ene-3-one 398 (M) + , 356, 274, 271, 229, 187, 173, 159, 147, 124 (100%), 121, 105, 95 Stigmast-4,22-diene-3-one 410 (M) + , 367, 349, 297, 269, 253, 227, 147, 124 (100%), 105, 91
(24R)-Stigmast-4-ene-3-one 412 (M) + , 397, 370, 288, 271, 245, 229, 187, 173, 159, 147, 124 (100%), 121, 105,
95, 79, 55 Ergost-4-ene-3,6-dione 412 (M) + , 385, 371, 285, 267, 243, 187, 175, 149, 137, 136, 121, 109, 95, 79, 55
(100%)
Trang 9Also, Kirk44 observed a mixture of epimers in peroxy-acid oxidation of 3-alkoxy-3,5-dienes
in the presence of an ethanol/water mixture where the β-isomer predominated He also stated that their proportions depend both upon the solvent and upon the method of mix-ing the reactants, aqueous-organic solvents, and gradual addition of peroxy-acid to the steroid, favoring the synthesis of the 6β-hydroxy-compound To the best of our knowl-edge, this is a new synthesis of stigma-4,22-dien-6β-ol-3-one that can be applied to steroids having an analogous nucleus This synthesis allowed us to confirm the structure of a minor compound
FIGURE 6.2
New triterpenoid synthesis.
TABLE 6.4
Physic and Spectroscopic Data of Synthesized Stigmast-4,22-dien-6-ol-3-one Isomers
Compound m.p./°C IR (cm –1 ) 1 HNMR [ δ (ppm)] 13 CNMR [ δ (ppm)]
Stigmast-4,22-dien-6β-ol-3-one 210–21
2
1690, 1660 5.79 (1H, s) (H-4) 200.56 (C-3), 168.75 (C-5),
5.15 (1H, dd) (H-23) 138.09 (C-22), 129.44 (C-23), 5.02 (1H, dd) (H-22) 126.21 (C-4), 73.12 (C-6) 4.34 (1H, s) (H-6)
Stigmast-4,22-dien-6α-ol-3-one
166–16 8
1680, 1655 6.16 (1H, s) (H-4) 199.61 (C-3), 171.81 (C-5),
5.14 (1H, dd) (H-23) 137.97 (C-22), 129.54 (C-23), 5.02 (1H, dd) (H-22) 119.62 (C-4), 68.75 (C-6) 4.33 (1H, m) (H-6)
Trang 106.3 Conclusions
Being predominantly a waste product, wheat straw proved to be an interesting and unex-plored source for chemical structures or models We have exunex-plored the triterpenoid and phenolic composition since these compounds appear to have a role in plant defense as sig-nal transducers and play a regulatory role in interactions of plants with their biotic envi-ronment Baas50 commented that “qualitative compounds or toxins” are structures that are biologically active at much lower concentrations, many of them even at hormone levels and usually they are oxidized compounds This is in good agreement with our experimen-tal data
Utilizing this naturally occurring chemical warfare among plants, wheat straw and other cover crops could play a more important role in controlling weeds in crops in the future In some cases, it is possible that herbicide use may be reduced by partial substitution of the naturally occurring phytotoxic chemicals in mulches, the latter possessing known ecologi-cal and economiecologi-cal advantages We believe that many no-till farmers are unconsciously receiving the benefits of allelopathy when they plant crops no-till into straw and certain cover crops If ethnopharmacology and its cultural heritage can contribute to the develop-ment of clinical drugs, so in a parallel way, popular agricultural practices may lead to the discovery of new natural agrochemicals
References
1 Theander, O., Review of straw carbohydrate research, Prog Biotechnol., 1, 217, 1985.
2 Kossila, V.L., Straw and Other Fibrous By-Products as Feed, Sundstol, F and Owen, E., Eds.,
Elsevier, Amsterdam, 1984, 4.
3 Theander, O and Aaman, P., Straw and Other Fibrous By-Products as Feed, Sundstol, F and
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