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Pregnancy and its outcome in women with and without surgical treatment of congenital heart disease.. Percutaneous mitral balloon valvotomy during pregnancy in a patient with severe mitra

Trang 1

75 Prifti E , Crucean A , Bonacchi M Early and long term outcome of the arterial switch operation for transposition of the great arteries:

predictors and functional evaluation Eur J Cardiothorac Surg 2002 ;

22 : 864 – 873

76 Clarkson PM , Wilson NJ , Neutze JM , et al Outcome of pregnancy after the Mustard operation for transposition of the great arteries with intact ventricular septum J Am Coll Cardiol 1994 ; 24 :

190 – 193

77 Lao TT , Sermer M , Colman JM Pregnancy following surgical

cor-rection for transposition of the great arteries Obstet Gynecol 1994 ;

83 : 665 – 668

78 Drenthen W , Pieper PG , Ploeg M , et al Risk of complications during pregnancy after Senning or Mustard (atrial) repair of complete transposition of the great arteries Eur Heart J 2005 ; 26 :

2588 – 2595

79 Ploeg M , Drenthen W , van Dijk A , et al Successful pregnancy after

an arterial switch procedure for complete transposition of the great

arteries Br J Obstet Gynaecol 2006 ; 113 : 243 – 244

80 Presbitero P , Prever SB , Brusca A Interventional cardiology in

pregnancy Eur Heart J 1996 ; 17 : 182 – 188

81 Teerlink JR , Foster E Valvular heart disease in pregnancy: a

contem-porary perspective Cardiol Clin 1998 ; 16 : 573 – 598

82 Canobbio MM , Mair DD , van der Velde M , et al Prengnacy outcomes

after the Fontan repair J Am Coll Cardiol 1996 ; 28 : 763 – 767

83 Drenthen W , Pieper PG , Roos - Hesselin JW , et al Pregnancy and delivery in women after Fontan palliation Heart 2006 ; 92 :

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Chapter 20

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129 Elkyam U , Tummala PP , Rao K , et al Maternal complications associated with subsequent pregnancy in women with history of

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96 Smith R , Brender D , McCredie M Percutaneous transluminal

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97 Glanz JC , Pomerantz RM , Cunningham MJ , et al Percutaneous

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98 Esteves CA , Ramos AI , Braya SL , et al Effectiveness of percutaneous

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99 BenFarhat M , Maatouk F , Betbout F , et al Percutaneous balloon

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100 Chow WH , Chow TC , Wat MS , et al Percutaneous balloon mitral

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101 Gangbar EW , Watson KR , Howard RJ , et al Mitral balloon

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102 Ribeiro PA , Fawzy ME , Awad M , et al Balloon valvotomy for

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103 Patel JJ , Mitha AS , Hussen F , et al Percutaneous mitral valvotomy

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104 Iung B , Cormier B , Elias J , et al Usefulness of percutaneous balloon

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109 Operative vaginal delivery ACOG Technical Bulletin Number 196 –

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111 Haas JM The effect of pregnancy on the midsystolic click and

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113 Arias F , Pineda J Aortic stenosis and pregnancy J Reprod Med 1978 ;

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158 Briggs GB , Bodendorfer JW , Freeman RK , Yaffe SJ , eds Drugs in Pregnancy and Lactation Baltimore, MD : Williams and Wilkins ,

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159 Oakley CM , Doherty P Pregnancy in patients after heart valve

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160 Antunes MJ , Myer IG , Santos LP Thrombosis of mitral valve pros-thesis in pregnancy: management by simultaneous caesarean section

and mitral valve replacement Case report Br J Obstet Gynaecol 1984 ;

91 : 716 – 718

161 Golby AJ , Bush EC , DeRook FA , et al Failure of high - dose heparin

to prevent recurrent cardioembolic strokes in a pregnancy patient

with a mechanical heart valve Neurology 1992 ; 42 : 2204 – 2206

162 Lev Ran O , Kramer A , Gurevitch J , et al Low - molecular - weight

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163 Ginsberg JS , Chan WS , Bates SM , et al Anticoagulation of pregnant

women with mechanical heart valves Arch Intern Med 2003 ; 16 :

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164 Leyh RG , Fischer S , Ruhparwar A , et al Anticoagulation for prosthetic heart valves during pregnancy: is low - molecular - weight

heparin an alternative? Eur J Cardiothorac Surg 2002 ; 21 : 577 – 579

165 American College of Obstetricians and Gynecologists Committee

Opinion: safety of Lovenox in pregnancy Obstet Gynecol 2002 ; 100 :

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166 Medwatch Safety Alert 2002 www.fda.gov/medwatch/SAFETY/ 2002/lovenox.htm

167 Vahanian A , Baumgartner H , Bas J , et al Guidelines on the manage-ment of valvular heart disease: the task force on the managemanage-ment of

valvular heart disease of the European Society of Cardiology Eur Heart J 2007 ; 28 : 230 – 268

168 Bates SM , Greer IA , Hirsh J , et al Use of antithrombotic agents during prengnacy: the seventh ACCP conference on antithrombotic

agents and thrombolytic therapy Chest 2004 ; 126 : 627 – 644

169 Rotmensch HH , Rotmensch S , Elkayam U Management of cardiac

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171 Phelps SJ , Cochran EC , Gonzalez - Ruiz A , et al The infl uence of gestational age and preeclampsia on the presence and magnitude of

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172 Schroeder JS , Harrison DC Repeated cardioversion during preg-nancy Treatment of refractory paroxysmal atrial tachycardia during

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173 Jaffe R , Gruber A , Fejgin M , et al Pregnancy with an artifi cial

pace-maker Obstet Gynecol Surv 1987 ; 42 : 137 – 139

174 Scott JR , Wagoner LE , Olsen SL , et al Pregnancy in heart transplant recipients: management and outcome Obstet Gynecol 1993 ; 82 :

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175 Branch KR , Wagoner LE , McGrory CH , et al Risks of subsequent pregnancies on mother and newborn in female heart transplant

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176 Kossoy LR , Herbert CM , Wentz AC Management of heart

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177 Key TG , Resnik R , Dittrich HC , et al Successful pregnancy

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Chapter 20

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Critical Care Obstetrics, 5th edition Edited by M Belfort, G Saade,

M Foley, J Phelan and G Dildy © 2010 Blackwell Publishing Ltd

Donna Dizon - Townson

Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT and Intermountain

Healthcare, Department of Maternal - Fetal Medicine, Provo, UT, USA

Pulmonary embolism (PE), albeit a rare event, remains the

leading cause of maternal mortality in the United States [1,2]

Furthermore, deep venous thrombosis (DVT) can cause signifi

-cant morbidity [3] Pregnancy - related venous thromboembolism

(VTE) has been reported to occur in approximately 0.5 – 3.0 per

1000 pregnancies based on studies using radiographic

documen-tation [4 – 6] Clinical symptomatology should be confi rmed with

objective testing Almost 75% of patients who present with

sus-pected thromboembolic disease, and are then subjected to testing

such as Doppler ultrasound or venography, are found not to have

the condition [7] When DVT is diagnosed and heparin

treat-ment instituted, the incidence of PE and maternal mortality can

be decreased by threefold and 18 - fold, respectively The goal of

this review is to facilitate the recognition of the clinical signs and

symptoms of VTE disorders, describe a rational approach to the

work - up of a suspected hypercoagulable state, and review the use

of various diagnostic and treatment modalities

Incidence and r isk f actors

Although many studies about maternal mortality cite PE as the

leading cause, they do not distinguish VTE from amniotic fl uid

or air embolism [8 – 10] At least half of these deaths are due to

thrombotic embolism [9,11 – 15] During 1991 – 1999, a total of

4200 deaths were determined to be pregnancy related The overall

pregnancy - related mortality ratio was 11.8 deaths per 100,000 live

births and ranged from 10.3 in 1991 to 13.2 in 1999 The leading

causes of pregnancy - related death were embolism (20%),

hemor-rhage (17%), and pregnancy - induced hypertension (16%) The

leading causes of death among women who died after a live birth

(60% of all pregnancy - related deaths) were embolism (21%),

pregnancy - induced hypertension (19%), and other medical

con-ditions (17%) 2 (Table 21.1 )

As illustrated in Figure 21.1 , from 1970 to 1985, maternal mortality rates from PE declined by 50% [9] The traditionally held view is that the maternal risk for VTE is greater in the imme-diate puerperium, especially following cesarean delivery Postpartum DVT has been reported to occur 3 – 5 times more often than antepartum DVT, and 3 – 16 times more frequently after cesarean as opposed to vaginal delivery [16,17] In contrast, Rutherford and associates found that the highest incidence of pregnancy - related VTE was not in the puerperium but in the fi rst trimester of pregnancy [18,19] (Figure 21.2 ) These authors also found that the risk of DVT did not increase with advancing ges-tational age but stayed relatively constant (see Figure 21.2 ) In contrast, PE (Figure 21.3 ) was almost twice as likely to occur in the postpartum patient and appeared to be related to the route

of delivery More recently, Gerhardt and colleagues reported on

119 women with a pregnancy - related VTE [20] Approximately half (62 women) experienced a DVT during pregnancy: 14 (23%)

in the fi rst trimester, 13 (21%) in the second trimester, and 35 (56%) in the third trimester The other half (57 women) experi-enced a DVT in the immediate puerperium: 38 (68%) following vaginal delivery and 19 (32%) following cesarean section In summary, pregnancy - related VTE may occur at any time during pregnancy or the immediate puerperium A recent 30 - year pop-ulation - based study of trends in the incidence of VTE during pregnancy and post partum confi rmed the signifi cant riks of VTE during the puerperium [21] Although the incidence of PE has decreased over time, the incidence of DVT is unchanged Therefore, regardless of gestational age, the clinician should have

a heightened awareness for the diagnosis when a gravid or post-partum woman presents with clinical symptomatology suspicious for VTE

Important risk factors for VTE during pregnancy are immobil-ity and bed rest “ Bed rest ” is often recommended for a variety

of obstetric disease such as threatened preterm labor or pre eclampsia The clinician should keep in mind the increased risk for VTE when making recommendations for limited maternal physical activity or long distance travel Traveling long distances

by air may also increase a pregnant woman ’ s risk of a PE

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Chapter 21

Additional risk factors in the gravid woman include surgery,

trauma or a prior history of superfi cial vein thrombosis [22]

Ethnic background and maternal age are important risk factors

for PE The overall mortality rate for black women was 3.2 times

higher than for white women In addition, women 40 years or

older were at a 10 times greater risk of mortality than women

under 25 for both ethnic groups [9] (Figure 21.4 ) Recent

preg-nancy surveillance has confi rmed that pregpreg-nancy - related

mortal-ity ratios continued to be 3 – 4 times higher for black women than

7

6

5

4

3

2

1

0

Date

Figure 21.1 Maternal deaths due to pulmonary embolism per 100,000 births

from 1970 to 1985 (Reproduced by permission from Franks AL, Atrash AK,

Lawson, et al Obstetrical pulmonary embolism mortality United States

1970 – 1985 Am J Publ Health 1990; 80: 720 – 722.)

70 60 50 40 30 20 10

0

Trimester

Figure 21.2 Distribution of deep venous thrombosis and pulmonary embolism

during each trimester of pregnancy: an 11 - year review (Reproduced by permission from Rutherford SE, Montoro M, McGehee W, et al Thromboembolic disease associated with pregnancy: an 11 - year review, SPO Abstract 139 Am J Obstet Gynecol 1991; 164: 286.)

Table 21.1 Causes of pregnancy - related death, by outcome of pregnancy and pregnancy - related mortality ratios ( PRMR * ) United States, 1991 – 1999

Cause of death Outcome of pregnancy (% distribution) All outcomes

Live birth Stillbirth Ectopic Abortion †

Molar Undelivered Unknown % PRMR (n = 2519) (n = 275) (n = 237) (n = 165) (n = 14) (n = 438) (n = 552) (n = 4200)

Total † † 100.0 100.0 100.0 100.0 100.0 100.0 100.0 100.0 11.8

* Pregnancy - related deaths per 100 000 live births

† Includes spontaneous and induced abortions

§ Pregnancy - induced hypertension

¶ Cerebrovascular accident

* * The majority of the other medical conditions were cardiovascular, pulmonary, and neurologic problems

† †

Percentages might not add to 100.0 because of rounding

Reproduced by permission from Chang J, Elam - Evans LD, Berg CJ, et al Pregnancy - related mortality surveillance – United States 1991 – 1999 MMWR 2003; 52(SSO2):

1 – 8

Trang 7

(unprovoked) had an antepartum recurrence rate of 5.9% (95%

CI 1.2 – 16%) It is assumed that the risk of recurrence diminishes

as the time from initial event increases The mean time from initial event to enrollment in the study was 4 years In another study, on a cohort of 1104 women with previous VTE, 88 of them became pregnant and did not receive thromboprophylaxis There were nine recurrences during pregnancy and 10 during the puer-perium, with a rate of 5.8% (95% CI 3.0 – 10.6) In pregnancy, the recurrence rate was 7.5% (95% CI 4.0 – 13.7) if the fi rst VTE was unprovoked, related to pregnancy or to oral contraceptive use, whereas no recurrence occurred if the fi rst VTE related to other transient risk factors [25]

Inherited and acquired thrombophilias are additional risk factors for VTE The inherited thrombophilias include defi cien-cies of protein C, protein S, and antithrombin III, factor V Leiden, prothrombin G20210A, and the 5,10 - methylenetetrahydrofolate reductase mutations The most commonly investigated acquired thrombophilia is the antiphospholipid syndrome

In summary, the risk of VTE varies among pregnant women, therefore individualization of management must be emphasized This risk will depend not only on the pregnancy, but also on additional clinical factors such as a prior history of thromboem-bolism, mode of delivery, prolonged immobilization, age, and ethnicity (Table 21.2 ) In the presence of a personal or familial history of VTE, testing for thrombophilia should be accom-plished to better defi ne the specifi c risk A comprehensive throm-bophilia work - up should include testing for functional defi ciencies

of protein C, protein S, and antithrombin III These tests should

be performed preferably when the patient is not pregnant and prior to anticoagulation In addition, molecular tests for factor V Leiden and the prothrombin G20210A mutation, which are unaf-fected by pregnancy or anticoagulation, should also be performed

To complete the evaluation, screening for antiphospholipid syn-drome with testing for IgG and IgM anticardiolipin antibodies and lupus anticoagulant should be included A positive test for antiphospholipid syndrome appears to carry the greatest impact

on maternal and fetal outcome in subsequent pregnancies

Normal h emostasis

Few systems are more complex than hemostasis Interactions among the vessel wall, platelets, and soluble molecules in the

for white women [2] In addition, the pregnancy - related

mortal-ity ratios for black women aged > 39 years were particularly high

in comparison with white women in the same age group [2]

Blood groups A and AB may be associated with an increased risk

for VTE during pregnancy [23]

A prior history of a VTE confers a greater risk for recurrence

especially if the initial event was idiopathic or associated with a

hereditary or acquired thrombophilia [24] In a prospective

cohort study investigating the risk of recurrence of pregnancy

related VTE in 125 women who had a history of VTE, heparin

was withheld antepartum but administered 6 weeks postpartum

in all women The antepartum recurrence rate was 2.4% (95% CI

0.2 – 6.9%) There were no recurrences in the 44 patients (0%:

95% CI 0.0 – 8.0%) who did not have thrombophilia and had a

previous episode of thrombosis that was associated with a

tem-porary risk factor Patients with a positive result for

thrombo-philia and/or a previous episode of thrombosis that was idiopathic

Deep venous thrombosis Pulmonary embolism

0

10

20

30

40

50

60

70

80

90

100

Cesarean section Vaginal delivery

Figure 21.3 The frequency of postpartum deep venous thrombosis and

pulmonary embolism according to route of delivery (Reproduced by permission

from Rutherford SE, Montoro M, McGehee W, et al Thromboembolic disease

associated with pregnancy: an 11 - year review Am J Obstet Gynecol

1991;164:286.)

20–24

White

Black

* Deaths per 100,000 live births

0

20

40

60

80

100

120

140

160

180

Age group (yrs)

Figure 21.4 Pregnancy - related mortality ration, by age and race – United

States, 1991 – 1999 (Reproduced by permission from Chang J, Elam - Evans LD,

Berg CJ, et al Pregnancy - related mortality surveillance – United States

1991 – 1999 MMWR 2003; 52(SSO2): 1 – 8.)

Table 21.2 Factors associated with a higher risk of pulmonary embolism

Maternal age Ethnic background Operative delivery Prior thromboembolism Prolonged immobilization Inherited/acquired coagulation disorders Trauma

Trang 8

Chapter 21

The intrinsic and extrinsic pathways lead to the fi nal common clotting pathway Both pathways are activated by components of the vessel wall and lead to activation of progressive exponential increase in subsequent factors In the intrinsic pathway, high molecular weight kininogen and kallikrein are cofactors for the initial step of the process, the activation of factor XII (XIIa) By catalyzing the formation of kallikrein from prekallikreins, factor XIIa also helps to initiate fi brinolysis, activate the complement system, and produce kinins [26] Factor XI is activated by XIIa and then cleaves factor IX to form IXa In comparison, the extrin-sic pathway is so named because this pathway relies on tissue thromboplastin as a cofactor Tissue thromboplastin is released into the circulation following membrane damage or proteolysis [26] Factor VII is then activated to VIIa which, with tissue thromboplastin, can activate factors IX or X The common pathway begins with activation of factor X by either VIIa or IXa,

in combination with the protein cofactor VIII:C (the antihemo-philic factor) and the calcium ion, on the platelet surface (to form

PF 3 ) Factor Xa, assisted by cofactor Va, enzymatically divides prothrombin into thrombin and a peptide activation fragment,

F 1 + 2 Separation from this fragment liberates thrombin into the

fl uid phase Thrombin catalyzes the formation of fi brin mono-mers from fi brinogen and, thus, releases fi brinopeptides A and B and facilitates activation of V, VIII:C, and XIII A fi brin gel is created by the hydrophobic and electrostatic interactions of the

fi brin α and γ chains Subsequently, factor XIIIa forms covalent bonds linking nearby α and γ chains to form a stable polymerized

fi brin clot into which water is also incorporated

Trapped within the clot are proteins that contribute to the enzymatic digestion of the fi brin matrix: plasminogen and minogen activators A variety of substances can activate plas-minogen Plasma plasminogen activator is activated by factor XIIa Release of tissue activators (tissue plasminogen activator) from blood vessel epithelium (especially venous) is stimulated by exercise, emotional stress, trauma, surgery, hypotensive shock, pharmacologic agents, and activated protein C [17,26,31] The

fi brinolytic enzymes streptokinase and urokinase also activate plasminogen [32] Having been activated from plasminogen, plasmin cleaves arginyl - lysine bonds in many substrates, includ-ing fi brogen, fi brin, factor VIII, and complement [32,33] The result of plasmin action on fi brin and fi brinogen is release of protein fragments, referred to as fi brinogen degradation products (or fi brin split products) The larger fragments, which may have slow clotting activity, are further divided by plasmin These frag-ments have anticoagulant activity, in that they inhibit the forma-tion and cross - linking of fi brin [26] Measurement of fi brin degradation products provides an indirect measurement of fi bri-nolysis α 2 antiplasmin, a specifi c plasmin inhibitor that binds to

fi brin and fi brinogen, is found in serum, platelets, and within the clot, along with other inhibitors of plasmin or plasminogen activity [32]

As a potent inhibitor of thrombin, antithrombin III (AT III)

is important in the regulation of hemostasis In decreasing affi n-ity, AT III binds and inactivates factors IXa, Xa, XIa, and XIIa

vicinity of an injury work to repair the vessel defect without

sacrifi cing nearby vessel patency The key processes are: (i)

vasoconstriction; (ii) formation of a platelet plug; (iii) formation

of a stable “ seal ” by coagulation factors; (iv) prevention of

spread of the clot along the vessel wall; (v) prevention of

occlusion of the vessel by clots when possible; and (vi)

remodel-ing and gradual degradation of the clot after it is no longer

needed

The maintenance of normal blood fl ow requires intact, patent

blood vessels After an injury, the hemostatic and fi brinolytic

systems work together to protect vascular integrity and assist in

repair Vessel wall integrity, platelet aggregation, normal function

of the coagulation cascade, and fi brinolysis are all vital to this

process The initial response to injury is vasoconstriction, which

reduces local blood fl ow and limits the size of the defect that the

thrombus is required to seal [26] After platelets begin to adhere

to the exposed vessel wall, they change shape and secrete the

contents of their granules This action leads to further platelet

accumulation or aggregation, and results in the formation of a

platelet plug

The numerous substances released by platelets include

throm-boxane A 2 (TxA 2 ), a potent vasoconstrictor and preaggregatory

agent [27,28] ; serotonin, a vasoconstrictor [28] ; and adenosine

diphosphate (ADP), which enhances platelet aggregation

Platelets also produce vascular permeability factor and platelet

growth factor, which stimulate fi broblasts and vascular smooth

muscles [17,26] Released platelet factor 4 (PF 4) and β

thromboglobulin are used as markers of platelet activity [29,30]

The platelet contractile protein, thrombasthenin, enables

secre-tion of these substances and also enhances clot retracsecre-tion [17] A

platelet surface phospholipoprotein, platelet factor 3 (PF 3 ),

becomes available to bind factor V to catalyze the formation of

thrombin Thrombin, in turn, potentiates platelet aggregation

[30]

Whereas TxA 2 is the result of platelet arachidonic acid

metabo-lism, arachidonic acid in endothelial cells is metabolized to

pros-tacyclin (PGI 2 ) Prostacyclin inhibits aggregation and stimulates

vasodilation, and thus counteracts TxA 2 by increasing cyclic

ade-nosine monophosphate (AMP) [26] Because PGI 2 is

concen-trated within the vessel wall, the greater the distance from the

lumen, the lower the concentration of PGI 2 and the higher the

concentration of proaggregatory substances As platelets begin to

seal a vascular defect, the coagulation cascade produces fi brin,

which is polymerized as clot and incorporated into the platelet

plug

Proteolytic cleavage or conformational changes activate the

circulating clotting factors at the site of injury Factors II, VII, IX,

and X require a vitamin K - dependent reaction in the liver in

which γ - carboxyglutamic acid residues are attached to the protein

structure This action provides a location to form a complex with

calcium ion and phospholipid receptors on the platelet or

endo-thelial cell membranes Subsequent steps in the clotting cascade

occur at those sites and include the formation of thrombin Once

formed, this is released into the fl uid phase

Trang 9

suggest ongoing increased fi brinolytic activity [40] Within an hour of delivery, this fi brinolytic potential decreases, as a result

of placental inhibitors [41] , and returns to normal These changes are believed to contribute to the hypercoagulability of the puer-perium [18,19] Levels of factors XI and XIII decrease When the placenta separates, tissue thromboplastin is released into the cir-culation, increasing the chance for thrombosis [42] Additional factors balancing the increased tendency toward coagulation may

be a pregnancy - specifi c protein (PAPP - A) which, like heparin, facilitates neutralization of thrombin by AT III [34] Platelet counts appear to remain in the normal range during pregnancy, but have been documented to be signifi cantly higher than prede-livery on days 8 and 12 after vaginal deprede-livery, and continued to rise 16 days after a cesarean delivery [43] The platelet count remained signifi cantly higher than predelivery values for 24 days after cesarean delivery [43]

Thrombophilias

Approximately half of the women who have a pregnancy - related VTE possess an underlying congenital or acquired thrombophilia [44] In almost 50% of patients with a hereditary thrombophilia, the initial thrombotic event occurs in the presence of an addi-tional risk factor such as pregnancy, oral contraceptive use, orthopedic trauma, immobilization or surgery [45,46]

Antithrombin III defi ciency, although the most rare of the congenital thrombophilias, is the most thrombogenic conferring

a 50% lifetime and pregnancy - related risk for thrombosis [47]

AT III defi ciency occurs in approximately 0.02 – 0.17% of the general population and 1.1% of individuals with a history of VTE Defi ciencies of protein C and protein S, although less thrombo-genic than AT III defi ciency, are more common [47] Carrier rates for defi ciencies of protein C and S are 0.14 – 0.5% in the general population In individuals who have had a history of VTE, 3.2% will have either protein C or protein S defi ciency

As a result of the Human Genome Project and major advances

in gene identifi cation, common genetic predispositions to thrombophilia, including factor V Leiden and the prothrombin G20210A mutation, have been described Resistance to APC is now known to be the most common genetic predisposition to

AT III acts as a substrate for these serine proteases but forms

stable intermediate bonds with the active portion and, thus,

neu-tralizes the respective enzyme [34] Heparin binds to AT III and

induces a conformational change that increases the affi nity of AT

III for thrombin The otherwise slow inactivation of thrombin by

AT III is accelerated greatly by even small amounts of heparin

After a stable thrombin – AT III complex is formed, heparin is

released and available for repetitive catalysis Excess amounts of

AT III are normally present in the circulation, and some are

bound to endothelial cell membranes via heparan, a sulfated

mucopolysaccharide with a function similar to heparin The

pres-ence of heparan on intact endothelial cell surfaces and its binding

to AT III, which neutralizes thrombin, help to prevent local

extension of the thrombus beyond the sites of vessel injury [35]

Defi ciency of AT III leads to a substantially higher incidence of

thrombotic events [36]

Proteins C and S are normally part of the protein C

anticoagu-lant system Like certain clotting factors, their synthesis depends

on vitamin K and involves addition of γ - carboxyglutamic acid

residues that enable binding, via calcium ions, to cell surfaces

Protein C is attached to endothelial cells, and protein S is attached

to endothelial and platelet membranes Endothelial cell surfaces

also have a specifi c protein receptor for thrombin –

thrombo-modulin The binding of thrombin to thrombomodulin, in the

presence of protein S, activates protein C (APC) and promotes

anticoagulation Complexes of APC and adjacently bound protein

S cofactor proteolyze the phospholipid - bound factors VIII:Ca

and Va This action results in a second mechanism to prevent

extension of the thrombus beyond the area of vessel injury [35]

Defi ciencies of either protein C or S are associated with an

increase in thromboembolic events [35,37] Homozygosity of

protein C defi ciency leads to fatal neonatal purpura fulminans

[38]

Changes in h emostasis in p regnancy

A century ago, Virchow described the triad of blood

hypercoagu-lability, venous stasis, and vascular damage conferring an

increased risk for thrombosis All these conditions occur during

pregnancy, thus conferring an increased risk for pregnancy

related VTE (Table 21.3 ) Estrogen stimulation of hepatic

synthe-sis of several procoagulant proteins increases with pregnancy

Levels of factors V, VII, VIII, IX, X, XII, and fi brinogen increase

muscle vascular relaxation and mechanical compression by the

gravid uterus occurs Placental separation and operative delivery

can cause endothelial vascular damage

Compensatory mechanisms such as concomitant rise in fi

bri-nolytic activity help to maintain coagulation equilibrium [39] As

pregnancy progresses, a low - grade chronic intravascular

coagula-tion results in fi brin deposicoagula-tion in the internal elastic lamina and

smooth muscle cells of the spiral arteries of the placental bed [40]

Increased fi brin split products and d - dimers during this period

Table 21.3 Hemostatic changes during pregnancy

Hemostatic changes promoting thrombosis Increased levels of factor V, VII, VIII, IX, X, XII, fi brinogen Placental inhibitors of fi brinolysis

Tissue thromboplastin released into the circulation at placental separation Venous stasis of the lower extremities

Endothelial damage associated with parturition Hemostatic changes countering thrombosis Decreased levels of factor XI, XIII Pregnancy - specifi c protein neutralizing AT III

Trang 10

Chapter 21

of 104 women with a median postthrombosis interval of 11 years revealed that 4% had ulceration, and only 22% were without complaints [57] Finally, it is important to remember that preg-nant patients commonly complain of swelling and leg discomfort and, as such, do not require objective testing in every instance It

is important to remember that the fi rst sign of DVT may be the occurrence of a PE In a similar manner, silent DVT has been found in 70% of patients with angiographically proven PE [58] During the initial evaluation in a pregnant patient with clinical symptomatology suspicious for a pregnancy - related VTE, risk factors as described above should be sought Again “ bed rest ” or limited physical activity, which is frequently recommended for a variety of obstetric diseases, is a common risk factor for VTE events

Diagnostic s tudies

Ultrasound

Non - invasive testing is usually the fi rst step in confi rming the diagnosis of DVT Real - time imaging with compression ultra-sound (CUS), including duplex Doppler, is the method of choice CUS uses fi rm compression with the ultrasound transducer probe

to detect an intraluminal defect Experience is required for accu-rate interpretation, and the affected leg should be compared with the unaffected one Maneuvers such as Valsalva (which distends the vein and slows proximal fl ow), release of pressure over a distal vein (which causes a rapid proximal fl ow of blood), and squeez-ing of the muscles all cause changes in Doppler shift Real - time imaging in the presence of DVT may detect a mass in the vessel lumen, a failure of the lumen diameter to increase with Valsalva

Alternatively, imaging may identify a hematoma, popliteal cyst or other pathology to explain the patient ’ s symptoms In a symp-tomatic non - pregnant individual, CUS has a sensitivity of 95% for proximal DVT (73% for distal DVT) and specifi city of 96% for detecting all DVT, with a negative predictive value of 98% and

a positive predictive value for 97% in the non - pregnant symp-tomatic patient [60] At least 50% of small calf thrombi are missed due to collateral venous channels [61,62] Repeating the examination within 2 – 3 days may reveal a previously latent clot During pregnancy, the iliac vessels are especially diffi cult to image This is due to pressure from the gravid uterus on the inferior vena cava As a result, Doppler fi ndings must be inter-preted cautiously In the puerperal patient, imaging may visualize

thrombosis [48 – 50] Eighty to 100% of cases of resistance to APC

are due to the factor V Leiden mutation This is a missense

muta-tion in the gene encoding factor V protein Individuals with factor

V Leiden have normal levels of factor V protein, but this protein

is resistant to the normal degradation by APC The abnormal

factor V protein fails to undergo the normal conformation change

required for the proteolytic degradation by APC Heterozygous

carriers have a sevenfold increase in the risk for venous

throm-bosis, whereas homozygous carriers have an 80 - fold increased

risk Carrier rates for factor V Leiden are 6 – 8% in northern

Europeans and 4 – 6% in US Caucasians [51,52] In the largest

prospective observational study, 134 heterozygous carriers for the

FVL mutation were identifi ed among 4885 gravidas (2.7%) with

both FVL mutation status and pregnancy outcomes available No

thromboembolic events occurred among the FVL mutation

car-riers (0%, 95% CI 2.7%) Three pulmonary emoboli and one

deep venous thrombosis occurred (0.08%, 95% CI 0.02 – 0.21%),

all in FVL non - carriers Thus, although the FVL is a rather

common mutation in the Caucasian population, the relative risk

of a pregnancy - related thromboembolic event in a heterozygote

carrier is low [53] Another mutation in the 3 ′ untranslated

region of the prothrombin gene, prothrombin G20210A, leads to

elevated prothrombin levels ( > 155%) and a 2.1 - fold increase in

the risk for thrombosis The prevalence of the mutation in the

Caucasian population is 2% The prevalence of the mutation is

6% among unselected patients with thrombosis and about 18%

in families with unexplained thrombophilia

Deep v enous t hrombosis

Clinical d iagnosis

In the gravid patient, DVT appears to occur more often in the

deep proximal veins and has a predilection for the left leg

[15,54,55] The clinical diagnosis of DVT [56] is diffi cult and

requires objective testing Of those patients with clinically

sus-pected DVT, half will not be confi rmed by objective testing Due

to the long - term implications of anticoagulant therapy and the

expense of a hypercoagulable work - up, clinical symptomatology

of VTE should usually be confi rmed with objective testing before

a diagnosis is rendered

Symptoms and signs of DVT are illustrated in Table 21.4

Swelling is considered whenever there is at least a 2 cm measured

difference in circumference between the affected and normal

limbs Homan ’ s sign is present when passive dorsifl exion of the

foot in a relaxed leg leads to pain, presumably in the calf or

pop-liteal areas The Lowenberg test is positive if pain occurs distal to

a BP cuff rapidly infl ated to 180 mmHg The presence of marked

swelling, cyanosis or paleness, a cold extremity or diminished

pulses signals the rare obstructive iliofemoral vein thrombosis

DVT has also signifi cant long - term implications, and a prior

history of DVT may affect the patient ’ s symptomatology Years

after a severe obstructive DVT, patients may experience

postphle-bitic syndrome (skin stasis dermatitis or ulcers) An investigation

Table 21.4 Clinical symptoms and signs of lower extremity deep

venous thrombosis

Unilateral pain, swelling, tenderness, and/or edema Limb color changes

Palpable cord Positive Homan ’ s sign Positive L ö wenberg test Limb size difference > 2 cm

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