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Process flow diagram for a potato chips industry 4.4.2 Wastewater sources and characterization Potato processing wastewater contains high concentrations of biodegradable components suc

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4.4 Potato processing industry

4.4.1 Process description

Food processing industry has grown rapidly parallel to the world population growth as a result of the inevitable necessity of the food to feed billions of people Potato is a very important and popular vegetable in human diet and its worldwide production has reached

to 314,2 million by 2008 (FAOSTAT, 2008) Various types of products such as potato chips, frozen French fries and other frozen food, dehydrated mashed potatoes, dehydrated diced potatoes, potato flake, potato starch, potato flour, canned white potatoes, prepeeled potatoes are processed from potato Due to the wide range of the products, the potato processing industries can differ in their process lines Although the type of processing unit depends upon the product selection, the major processes in all products are storage, washing, peeling, trimming, slicing, blanching, cooking, drying, etc The process line of a potato chips manufacturing plant is given in Fig 9

Fig 9 Process flow diagram for a potato chips industry

4.4.2 Wastewater sources and characterization

Potato processing wastewater contains high concentrations of biodegradable components such as starch and proteins, in addition to high concentrations of COD, TSS and TKN Therefore, wastewater production and composition of potato processing plants depend on the processing techniques to a large extent (Senturk et al., 2010a) Raw potatoes must be washed thoroughly to remove sand and dirt prior to other processes Water consumption

for fluming and washing varies from 18,5 to 7,9 liters per ton of potatoes Peeling of potatoes contributes the major portion of the organic load in potato processing waste Among three different peeling methods (abrasion peeling, steam peeling and lye peeling), lye peeling is the most popular peeling method used today Therefore, lye peeling wastewater is the most troublesome potato waste due to very high pH (11–12), high organic content mostly in colloidal form (Hung et al., 2006) The wastewater flows from different potato processing industries were reported as 17 m3/ton potato processed (Hung et al., 2006), 5-8 m3/ton potato processed (Guttormsen & Carlson, 1969), 3,9 m3/ton potato processed (Austerman-Houn & Seyfried, 1992) and 5,8 m3/ton potato processed (Cooley et al., 1964) Several publications on the characteristics of wastewaters resulting from various types of potato processing plants are summarized in Table 4

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Reference Parameter 1 Unit et al., 2010b)(Senturk (Wang et al., 2009) (Kalyuzhnyi et al., 1998) (Hadjivassilis et al., 1997) Houn & Seyfried, (Austerman-

1992) Industry - Potato chips 2 Potato starch Potato maize Potato chips Potato chips 3

1 TS: Total solids; TVS: Total volatile solids

2 Potato peeling and cutting process wastewater

3 Process wastewater which is a mixture of potato washing water after sand separation and potato fruit water after starch recovery

4 Values in paranthesis represent the average values

Table 4 Characteristics of wastewaters resulting from various types of potato processing

4.4.3 Anaerobic treatment applications for the treatment of potato processing

wastewaters

Senturk et al (2010a) investigated the mesophilic anaerobic treatment of potato processing wastewater obtained from a factory producing potato chips, maize chips and other snacks They used a laboratory scale mesophilic anaerobic contact reactor which had similar features with activated sludge systems The reactor was operated at different OLRs and HRTs ranging from 1,1–5,0 kg COD/m3.day and 5,11–1,06 day, respectively, and it achieved COD removal efficiencies between 78–92% Furthermore, various kinetic models such as Monod first order model, Stover–Kincannon model, Grau second-order and Michaelis–Menten models have been applied to the experimental data in order to determine substrate balance, maximum utilization rate and volumetric methane production The applied models showed good agreement (R2>0,98) with the experimental data and methane yield was determined as 0,394 L CH4/g CODremoved

A novel anaerobic–aerobic integrative baffled bioreactor supplied with porous burnt-coke particles was developed for the treatment of potato starch wastewater by Wang et al (2009) This bioreactor was found to be effective for the removal of COD (88,4–98,7%) and NH3–N (50,4 to 82,3%), in high-strength starch wastewater

Musluoglu (2010) studied the co-digestion of potato chips production industry waste with

the waste activated sludge from two different full-scale facilities Average biogas potentials

in both completely mixed reactors were between 600-650 m3/ton VSadded

The performances of laboratory scale UASB (0,84 L) and anaerobic packed-bed reactors (APB) (0,7 L) treating high strength potato leachate were compared by Parawira et al (2006)

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The maximum OLRs that could be applied to the UASB and APB reactors for stable operation were approximately 6,1 and 4,7 g COD/L day, respectively More than 90% COD removal efficiency was reported for both type of reactors On the contrary to the results obtained by Linke (2006) at an anaerobic completely mixed reactor treating solid potato waste, the methane yield increased with increasing organic loading rate up to 0,23 L CH4/g CODdegraded in the UASB reactor and 0,161 CH4/g CODdegraded in the APB reactor

The effect of recirculation rate on packed bed reactors (1 L) treating potato leachate at different OLRs ranging between 4–12 kg COD/day was studied by Mshandete et al (2004) The methane yield for the bioreactor with the lower recirculation flow rate (10 mL/minute) ranged between 0,10-0,14 m3 CH4/kg CODremoved, while for the other bioreactor it was between 0,14–0,20 m3 CH4/kg CODremoved Lower methane yields were achieved at higher OLRs While the methane yield of the reactor operated at high recirculation rate was more than the other bioreactor, in terms of process stability the reactor operated at low recirculation rate was superior Process failure, indicated by low pH, high volatile fatty acid (VFA) concentration, was experienced at an OLR of 12 kg COD/m3.day in the reactor operated at high recirculation rate This was attributed to the high recirculation flow rate which provided rapid mixing and fast diffusion of the accumulated VFAs into the biofilm where microbes were accumulated

The efficiency of the UASB process for the treatment of raw and pre-clarified potato maize waste up to the OLR of about 13-14 g COD/L.day was illustrated by Kalyuzhnyi et al (1998) Although the reactor performed high COD removal efficiencies (63-81%) for raw potato maize waste (PMW), some problems such as excessive foaming and sludge flotation were experienced due to the accumulation of undigested ingredients at high OLR (> 10 g COD/L.day) and moderate HRT (> 1 day) These problems were eliminated by the application

of shorter HRTs in order to enable better washout of light ingredients that were accumulated

in the reactor, or by temporarily decreasing OLR Methane yield varied from 0,24 to 0,44 L/g CODremoved for raw PMW and from 0,30 to 0,37 L/g CODremoved for pre-clarified PMW

The anaerobic treatability of potato processing effluents by an anaerobic contact reactor operated at thermophilic conditions was studied by Senturk et al (2010b) The OLR of the reactor was gradually increased from 0,6 kg COD/m3.day to 8,0 kg COD/m3.day by decrementing the HRT from 9,2 days to 0,69 days The reactor could be operated at high OLRs without process failure and the average COD removal efficiency obtained at 8,0 kg COD/m3.day was 86% The average methane gas production was reported as 0,42 m3

CH4/kg CODremoved and the methane content in the biogas ranged between 68–89%

The performance of two-stage anaerobic digestion of solid potato waste under mesophilic and thermophilic conditions was evaluated by Parawira et al (2007) A solid bed reactor was used as the hydrolytic stage of the two staged process An UASB reactor fed with the leachate obtained from the hydrolysis reactor was used in the second step of the two-stage system with three different temperature combinations (mesophilic+mesophilic, mesophilic+thermophilic, thermophilic+thermophilic) They found that the methane yield

of the mesophilic system (0,49 m3 CH4/kg CODdegraded) was significantly higher than the thermophilic system (0,31 m3 CH4/kg CODdegraded) However, thermophilic operation reduced the complete digestion period of the waste (from 36 to 25 days) and higher OLRs

up to 36 kg COD/m3.day could be applied to the UASB reactor

The biogas yield of a completely stirred reactor treating solid potato waste at thermophilic conditions was found as 0,85–0,65 L/g TVS for the OLRs in the range of 0,8–3,4 g TVS/L.day, respectively (Linke, 2006) The results indicated a gradual decrease in the biogas

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yield and methane content (from 58% to 50%) of the biogas depending on the increase in the OLR of the reactor

The performance of two types of two-stage systems, one consisting of a solid-bed reactor connected to an UASB reactor, and the other consisting of a solid-bed reactor connected to a methanogenic reactor packed with wheat straw biofilm carriers, were investigated by Parawira et al (2005) While the performance in terms of methane yield was the same (0,39

m3 CH4/kg VSadded) in the straw packed-bed reactor and the UASB reactor, the packed-bed reactor degraded the potato waste in a shorter time due to the improved retention of methanogenic microorganisms in the process

4.5 Opium alkaloid industry

4.5.1 Process description

Opium is known to contain about 26 types of alkaloids such as morphine, narcodine, codein, papvarine and thebain (Sevimli et al., 1999) There are many different methods for the extraction of alkaloids from natural raw materials Most of the methods depend on both the solubility of the alkaloids in organic solvents and solubility of their salts in water (Hesse, 2002) The process flow scheme of a wet-mill opium alkaloid industry, which mainly consists of grinding, solid-liquid and liquid-liquid extraction and crystallization processes, was given in Fig 10

Fig 10 Process flow diagram for an opium alkaloid industry

Firstly opium poppy capsules are grinded and treated with an alkaline solution (lime), and then the slurry is pressed to extract the liquid that contains the alkaloids The pH of the liquid is adjusted to 9,0 and the impurities are separated by a filtration process In the extraction process, the alkaloids are extracted with acetic acid solution and other organic solvents such as toluene and butanol The morphine is crystallized by adding ammonium

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and separated from the solution by centrifuges The used solvents and the water are sent to the distillation column in order to recover toluene, alcohol groups and the remaining wastewater is treated in a wastewater treatment plant (Sevimli et al., 1999)

4.5.2 Wastewater sources and characterization

Opium alkaloid industry wastewaters are highly polluted effluents characterized with high concentrations of COD (mainly soluble), BOD5 and TKN, dark brown colour and low pH Alkaloid industry wastewaters are generally phosphorus deficient; therefore phosphorus addition might be required for biological treatment Soluble COD content and acetic acid related COD of the wastewater can be as high as 90% and 33%, respectively (Aydin et al., 2010) Sevimli et al (1999) determined the initial soluble inert COD percentage of opium alkaloid industry wastewaters as 2% Aydin et al (2010) reported the initial soluble and particulate inert COD content of opium alkaloid industry wastewaters under anaerobic conditions as 1,64% and 2,42%, respectively Although no available data could be found in the literature for the sulphate content of the alkaloid industry wastewaters, it may be present at high concentrations due to the addition of sulphuric acid at the pH adjustment stage Ozdemir (2006) reported a sulphuric acid usage of 48,3 kilograms per ton of opium processed Furthermore, the alkaloid wastewaters might contain some toxic organic chemicals such as N,N-dimethylaniline, toluene which are inhibitory for biological treatment (Aydin et al., 2010) The general characteristics of opium alkaloid plant effluents given in the literature are presented in Table 5

Reference Parameter Unit

Deshkar

et al

(1982) COD mg/L 30000-43078 18300–42500(25560) 22000-34780 36500 21040 18800 Soluble

COD

mg

BOD 5 mg/L 16625-23670 4250–22215(12000) 21250 32620 12075 15000 Alkalinity mg/L - 315–4450 (1290) 144-1050 - 4450 -

1 Numbers in parenthesis represent the median values

2 After coarse filtration

Table 5 Characteristics of opium alkaloid industry effluents

4.5.3 Anaerobic treatment applications for the treatment of opium alkaloid

wastewaters

Sevimli et al (2000) investigated the mesophilic anaerobic treatment of opium alkaloids industry effluents by a pilot scale UASB reactor (36 L) operated at different OLRs (2,8 – 5,2

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kg COD/m3.day) at a HRT of 2,5 days Although they experienced some operational problems, COD removal efficiency of 50–75% was achieved throughout the operational period One of the most detailed and long termed study on the anaerobic treatability of effluents generated form an opium alkaloids industry was presented by Aydin et al (2010) The treatment performance of a lab-scale UASB reactor (11,5 L) was investigated under different HRTs (0,84–1,62 days) and OLRs (3,4–12,25 kg COD/m3.day) at mesophilic conditions Although, the COD removal efficiency slightly decreased with increasing OLR and decreasing HRT, the reactor performed high COD removal efficiencies varying between 74%–88% Furthermore, a severe inhibition caused by N,N-dimethylaniline, coming from the wastewater generated in the cleaning operation at the derivation unit tanks of the industry, was experienced in the study During the inhibition period the treatment efficiency and biogas production dropped suddenly, even though the OLR was decreased and HRT was increased as a preventive action Despite these interventions, the reactor performance could not be improved and the reactor sludge had to be renewed due to the irreversible inhibition occurred for four months The reactor could easily reach to the same efficiency level after the renewal of the sludge Average methane yield of the opium alkaloids industry wastewater was reported as 0,3 m3 CH4/kg CODremoved Dereli et al., (2010) applied Anaerobic Digestion Model No.1 (ADM1), a structured model developed by IWA Task Group (Batstone et al., 2002), for the data obtained by Aydin et al (2010) ADM1 was able to simulate the UASB reactor performance in terms of effluent COD and pH, whereas some discrepancies were observed for methane gas predictions

Ozdemir (2006) investigated the co-digestion of alkaloid wastewater with acetate/glucose

by batch experiments, therefore the usage of these co-substrates did not improve removal efficiency significantly but acclimation period of microorganisms was reduced Continuous anaerobic treatment of alkaloid industry wastewater was further investigated by Ozdemir (2006) using three lab scale UASB reactors (Reactor 1: fed with alkaloid wastewater after hydrolysis/acidification, Reactor 2: fed with raw alkaloid wastewater, Reactor 3: fed with alkaloid wastewater together with sodium acetate as co-substrate) operated at different OLRs (2,5–9,2 kg COD/m3.day) and a HRT of 4 days Although all of the reactors performed well at low OLRs (~80% COD removal efficiency), process failure was experienced in R1 and R2 reactors at the OLR of 9,2 kg COD/m3.day

Ozturk et al (2008) studied the anaerobic treatability for the mixture of wastewater generated from the distillation column and domestic wastewater of an alkaloid industry by

a full-scale anaerobic Internal Cycling (IC) reactor with an OLR of 5 kg COD/m3.day COD and VFA removal efficiencies were 85 and 95%, respectively Biogas production rate of 0,1-0,35 m3 CH4/CODremoved was obtained The main problems stated in this study were high salinity and sulphate concentrations

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Wastewater

Type

Reactor Type/Operating

Temperature ( 0 C)

Capacity (m 3 ) (kgCOD/m OLR 3 day)

COD removal (%)

Methane yield (m 3 /kg COD) Reference

Pulp and Paper Baffled/35 0,01 5 60 0,141-0,178 (Grover et al.,

1999) Pulp and Paper Anaerobic

Contact/- - - 80 0,34 (Rajeshwari et al., 2000) Slaughterhouse UASB/- 450 2,1 80 - (Del Nery et al.,

2001)

Cheese Whey Baffled/35 0,015 - 94-99 0,31 (Antonopoulou et

al., 2008) Cheese Whey Upflow Filter/35 0,00536 - 95 0,55 (biogas) (Yilmazer &

Yenigun, 1999) Textile UASB/35 0,00125 - >90 - (Somasiri et al.,

2008) Textile Fluidized Bed/35 0,004 3 82 - (Sen & Demirer,

2003) Coffee Hybrid

(Bello-Mendoza & Castillo-Rivera, 1998)

Coffee UASB/35 0,005 10 78 0,29 (Dinsdale et al.,

1997) Brewery Sequencing

Batch/33 0,045 1,5-5 >90 0,326 (Xiangwen et al., 2008) Brewery AF/34-39 5,8 8 96 0,15 (Leal et al., 1998)

Bed/35 0,06 8,9-14 75-87 0,34 (Anderson et al., 1990) Olive Oil UASB/37 - 12-18 70-75 - (Azbar et al., 2010) Olive Oil Hybrid

(UASB +AF)/35 - 17,8 76,2 - (Azbar et al., 2010) Sugar Mill UASB/33-36 0,05 16 >90 0,355 (Nacheva et al.,

2009) Sugar Mill Fixed Bed/32-34 0,06 10 90 - (Farhadian et al.,

2007) Distillery Granular bed-

Baffled/37 0,035 4,75 80 - (Akunna & Clark, 2000) Distillery Fixed Film/37 0,001 23,25 64 - (Acharya et al.,

2008) Table 6 Anaerobic treatment applications for different industrial wastewaters

the previous sections Besides, it has a wide potential for wastewater treatment applications

of many industries such as pulp and paper, slaughterhouse, cheese whey, textile, coffee, brewery, olive oil, sugar mill, distillery, etc It is not possible to present all industrial wastewater treatment application examples of anaerobic digestion in a chapter; instead, examples from a number of selected studies were given in Table 6

5 Conclusions and future perspectives

Anaerobic biotechnology has a significant potential for the recovery of biomethane by the treatment of medium and/or high strength wastewaters especially produced in agro-industries By using this technology, ~ 250-300 m3 biomethane can be recovered per ton CODremoved depending on the inert COD content of the substrate COD removal rates are generally between 65-90% in these systems Anaerobic biotechnology, when used in the first

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treatment stage, provides the reduction of aeration energy and excess sludge production in the followed aerobic stage, thus increasing the total energy efficiency of the treatment plant Besides, it contributes to the increase in the treatment capacity of the aerobic stage Also it is possible to obtain a considerable increase of production capacity for an industry if an anaerobic first stage treatment is applied before aerobic stage in an industrial wastewater treatment plant treating medium strength organic waste In case of nitrogen removal in a two-stage (anaerobic+aerobic) biological wastewater treatment process, it may be necessary

to bypass some of the influent stream from anaerobic to aerobic stage in order to increase the denitrification capacity Autotrophic denitrification with H2S in the biogas is an important option that should be kept in mind to reduce organic carbon requirement for denitrification in two-stage treatment process treating wastewaters that contains high organic matter and high nitrogen (Baspinar, 2008) It is more appropriate to apply pre-treatment as phase-separation (two-staged) for industrial wastewaters containing high sulphate concentration

There are many full-scale applications for the operation of anaerobic processes under mesophilic (27-30 0C) and high pH conditions, especially for the treatment of high strength wastewaters with high nitrogen content In such conditions, full nitrification but partial denitrification at aerobic stage or an innovative nitrogen removal technology, Sharon/Anammox process, may be applied

sub-Another option for the pre-treatment of wastewater streams containing high COD (>40000 mg/L), total dissolved solids (TDS), TKN and potassium is an evaporation process that useful material can be recovered and residual condensate may be further treated by an anaerobic process

Recently, co-digestion applications of treatment sludge with other organic wastes have increased dramatically due to the subsidies for renewable energy produced from wastes In this respect, organic solid wastes and biological treatment sludge can be co-digested by installation of anaerobic co-digesters at the same location with available industrial-scale

anaerobic bioreactors or near the sources of wastes to be digested

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