Tiêu chuẩn iso 03400 1997 scan

For ew or d mater s of electr otec nical stan ar dization.. ar e for inf or mation only... How ev er , this method is also a plca le to the an an a pr opr iate note made in the expr es i

STA NDA RD

3400

Third e itio

19 7-0 - 15

Mkthode sp ctr omktr i ue

IS0 3 0 :19 7(E)

For ew or d

mater s of electr otec nical stan ar dization

ar e for inf or mation

only

0 IS0 19 7

Al rig ts r es r v ed Unle s oth r w is spe if ie , n p r t of this publcatio may be

r eprod c d or utiz d in an for m or by an mean , ele to ic or me hanical

publs er

Inter natio al Or ganizatio for Stan ar dizatio

Cas po tale 5 l C -121 Ge ev e 2 l Sw itz rlan

Inter net c ntr al@is c

x.40

c=c ; a 40 n t ; p is ; o=is c ; s=c ntr aI

Printe in Sw itz rlan

i

Cigar et es -

nor maly car r ied out in ac or dan e w ith IS0 4 8 How ev er , this method is also a plca le to the

an an a pr opr iate note made in the expr es ion of r es lts

2 Nor mativ e r ef er en es

v ald A ll stan ar ds ar e s bject to r ev ision, an p r ties to agr eements b sed on this Inter national

stan ar ds lsted b low Memb r s of IEC an IS0 maintain r egister s of c r r ently v ald Inter national

Stan ar ds

IS0 3 9 :19 7, Water for analytical la or ator y u e -

IS0 4 8 :19 1, Cigar etes

3 Pr i ciple

of an alq ot p r tion of the solution in tw o ste s A cidification of the solution w ith a miner al acid

same solution str on ly alkalne an disti ation of the alk loid Sp ctr ometr i me s r ement of

expr es ed as nicotine

4 Re gents

5 A pp r atu

5.1 Ste m disti ation a p r atu , con istin of the p r ts lsted in 5.1.1 to 5.1.4

the disti ation r ate

ar e the same

a ste m inlet at its b se

disti ation

2

I is imp r tant that d r i g the intr od ction of the sodium h dr oxide solution (4.2) ste m do s not

es a e thr ou h the ta or sto p r

5.7 Fiter p p r , fast-fiter i g gr ade, of a diameter to s it the glas f un el (5.6)

6 Pr oced r e

6.1 Pr ep r ation of test sample

6.2 T est p r tion

to a out 3 mg to 5 mg of alk loid

6.3 Disti ation

that the r es lts o tained ar e the same

Intr od ce the test p r tion (6.2) into the disti ation c amb r of the disti ation a p r atu (5.1) A dd

3

temp r atur e, diute to the mar k (v olume l/J w ith w ate Mix an u e this solution to deter mine

NOT E 6 I fitr ation is neces ar y, either the fir st 15 ml of f iltr ate s ould b dis ar ded o

to 2 0 ml w ith w ate

I the a sor ban e at 2 9 nm ex e d 0,8, diute an alq ot p r tion (I/.‘) of the disti ate to a s ita le

v olume (V’) as the alq ot p r tion (VJ If the a sor ban e at 2 9 nm is les than 0,2 u e lar ger cels

(5.3)

cigar ete, f or e c smokin r un is giv en by the f or mula

H wV,V,

-

nit -

a/VJp

(1)

w her e

A 23 + A 28

2

(2)

I is the o tical p th len th of the cel, in centimetr es;

y is the alq ot p r tion of I/, u ed for the disti ation, in mi ltr es;

V2 is the v olume of disti ate fr om the alkalne disti ation, in mi ltr es;

Stoper

-

I

I_-

i

-

A

h

15

-

T horpe c n e s r

Q, e t 6

A

-

Q> e t 54

@ e t 3

@ e t 8

Fr ont v iew

NOT ES

@ IS0

Electrical t

7

Dimen ion in mi metr es

A da ter for th

l q id sample

e t 3

- PT E

bor e 3

C 2

@ e t 10

E

0

r

c

:

Steam

-L

_-

@ e t 9

R C 2 ’

Q, e t 8

R S 2

@ e t 6

Stan ar dc n e s r a nd

distitlate r ec iv er

NOT E - A ll glas is medium w al b r osi cate

Annex B

[Z] W ilets C O.,

S w ain M L., Con ely J A , Br i e B A A nalytical Chemistr y, 2 , No 3, Mar ch

19 0, p 4 0-4 3

la or ator ies

ICS 6 160

De c ptor s: tobc o, cigar ete , smoke, c emical analyis, d ter minatio of c onte t, alk aloid , spe tometic meth d

Pric bs d o 9 pa ge