INTR ATIO A L O GNIZATIO FO STADR IZATIO l MEXY HA J.HI OI-‘AW3A UW5I l-IO CTAAPTM3A~WW.O GNlSTiO INTR ATIO AL DE N RMALISTIODesc riptors : animal prodc ts, met cemica l a na ly sis, det
Trang 1INTR ATIO A L O GNIZATIO FO STADR IZATIO l MEXY HA J.(HI OI-‘AW3A UW5I l-IO CTAAPTM3A~WW.O GNlSTiO INTR ATIO AL DE N RMALISTIO
Desc riptors :
animal prodc ts, met cemica l a na ly sis, determination of content phosphoru
Price bsed on 3 pges
Trang 2of n tio al sta d rds institutes (IS0 Memb r Bo ies) Th work of d v lo ing
A pri 19 1
Portu al
South A frica, Re of
Sp in
Th i la d
Turk y
0 International Organization for Stan ardization, 194 l
Printed in Sw itzerlan
Trang 3Meat and meat products - Determina tion of tota l phosphorus
me t a d mea t pro u ts
a sh
IS0 310 , Me t a d mea t pro u ts - Sa mplng 1 )
3 DE INIT IO N
cla use IO
qua lity Disti e water or water of e uiv a le t purity sha ll
5.2 Nitric a cid, pzO I4 g/ ml
5.3 Precipita ting rea ge t
wa ter a d a d 8 ml of nitric a cid (5.2)
stiring
5.3.4 To 10 ml of water a d s c es iv ely 3 ml of nitr ic
a cid (5.2) a d 5 ml of disti e quin ln
stiring L a e for 2 h a t ro m temp ra ture
F ilter, a d 2 0 ml of aceto e a d diute to 1 0 0 ml with
water
da rk
pla te with h les of dia meter n t ex e ding 4 m
6.2 Ana lytica l ba la nce
in a inc lin d p sitio in s ch a w ay tha t th source of h at
b low th lev el of th lquid F or h a ting by gas, a s ita ble
d v ice is a pla te of a sb stos prov id d with a circ la r h le,
fla me
6.6 F rite gla ss fiter, p re dia meter 5 to 15 pm (P 16)
1) At pr esent a t the sta ge of draft
Trang 4Tra nsfer th lquid qua ntita tiv ely into a 2 0 ml b aker or
co ica l fla sk, rinsing th fla sk (6.3) with wa ter A dd 10 ml
of nitric a cid T he tota l lquid v olume sh uld th n b a bout
6.8 Co ica l s ctio fla sk
6.9 Desic a tor, prov id d with a efectiv e d sic a nt
b aker or co ica l fla sk
6.10 Pasteur pip te
Co er with
a w atch gla ss a d b oi for 1 min o a h tpla te
place in th e s ctio d v ice (6.5)
Proce d from a re rese ta tiv e sa mple of a t lea st 2 0 g, Se
IS0 310
Alow to co 01 to
in s ch a w ay
ch nge in comp si tio
a re prev ente
w ash a y rema ining precipita te from th co ica l fla sk o to
th fiter
co ta in r a d store it in s ch a w ay tha t d teriora tio a d
th meth d giv en in 8.2 to 8.4, or cla use IO
1 h
NOT - I the mas of the dried prec ipita te
r epa t the a na ly xis wi th a sma ller test prtion
is more tha n 7 mg,
with a fork im e ia tely b fore ta king th test p rtio
8.5 Bla nk test
Cary out a bla nk test in pa ra llel with th a na ly is itself, u ing
rea ge ts, but omiting th test p rtio 8.2 T est p rtio
Weigh, to th n a rest 0,0 1 g, a bout 3 g of th test sample
into th fla sk (6.3) Se a lso th n te to 8.4,
8.3 Min ra liza tio
b oi ng chips
p rce ta ge
mas , by
me ns of t h formula
(5.1)
10
0,0 2 0 x m, x
ml
’ h at somew hat more stro gly A s so n as th mixture sta rts
to ca rb nize, a d more nitric a cid by me ns of a Pa steur
where
m
is th mas , in gra ms, of th test p rtio ;
“I
p os
lp o
moly
b a te precipi
ta te
quin ln
h s
F ina lly , w he th lquid
white fumes a pp a r
h at unti
re ea ta bi ty a re sa tisfie (se 9.2)
fla sk)
2
Trang 59.2 Re ea ta bi ty
same a naly t sha ll n t b grea ter tha n 0,0 g of p osp oru
Take up th ash in 15 ml of co ce trate nitric a cid (5.2),
u ing a stiring ro to a id dis olutio Tra nsfer th lquid to
a 2 0 ml co ica l fla sk Wash th a shing dish a d th stiring
b oi
proce d a ccording to
8.4
ng wa ter ba th Alow to co l a d
o ta in d it sha ll a lso me tio a y o era ting co ditio s n t
o tio a l,
d ta ils re uire for
complete
3
Trang 6T his p ge intentiona lly lef blank