[...]... Proteoglycans on Proteinase Activities Steven Georges, Dominique Heymann, and Marc Padrines 21 Proteoglycans and Osteolysis Marc Baud’Huin, Céline Charrier, Gwenola Bougras, Régis Brion, Frédéric Lezot, Marc Padrines, and Dominique Heymann 22 Proteoglycans and Cartilage Repair Mohamed Ouzzine, Narayanan Venkatesan, and. .. Jin-ping Li and Dorothe Spillmann PART IV 161 201 219 239 PROTEOGLYCANS INVOLVEMENT IN PATHOPHYSIOLOGY 17 Models for Studies of Proteoglycans in Kidney Pathophysiology Scott J Harvey 18 Lumican Promotes Corneal Epithelial Wound Healing Chia-Yang Liu and Winston Whei-Yang Kao 19 Shedding of Cell Membrane-Bound Proteoglycans Eon Jeong Nam and Pyong Woo... T., Ho, P L., and Fitch, F (1980) Clonal antibodies for core protein of chondroitin sulfate proteoglycan, Proc Natl Acad Sci 77, 3971–3973 Dorfman, A D., Vertel, B M., and Schwartz, N B (1980) Immunological methods in the study of chondroitin sulfate proteoglycans., Dev Biol 14, 169–198 Krueger, R C., Fields, T A., Mensch, J R., and Schwartz, N B (1990) Chick cartilage chondroitin sulfate proteoglycan... sequence and have generated transgenic animals to study aggrecan function in vivo This methodology could be adapted to cloning other cDNA sequences, including those of other large proteoglycans 2 Materials General: 1.5-mL microcentrifuge tubes and 1–200- and 100–1,000-mL pipettor tips were from USA Scientific (all are RNase/DNase/ pyrogen-free as supplied, autoclave if sterility is desired) 1 Proteoglycans:... employed, affording ease of producing staged embryonic material and of experimental access to live embryos; a lethal recessive mutation, nanomelia, provided a natural aggrecan knock-out model (12, 13) Much work has also been done in mice, which also have natural knock-out mutant models Françoise Rédini (ed.), Proteoglycans: Methods and Protocols, Methods in Molecular Biology, vol 836, DOI 10.1007/978-1-61779-498-8_1,... Jean-Claude Madelmont, and Jean-Michel Chezal PART III 171 183 PGS: MULTIFUNCTIONAL CELL REGULATORS 14 Serglycin: The Master of the Mast Cell Elin Rönnberg and Gunnar Pejler 15 Analysis of Aggrecan Catabolism by Immunoblotting and Immunohistochemistry Peter J Roughley and John S Mort 16 Heparan Sulfate Proteoglycans as Multifunctional... aggrecan cDNA domain structure and aggrecan structure The drawings indicate the approximate locations of the globular domains (G1, G2 and G3) and the keratan sulfate (KS) and chondroitin sulfate (CS) attachment regions An arbitrary number of CS and KS chains is represented by vertical lines available: the cartilage matrix deficiency (cmd) alleles (14, 15) These models and the ability to genetically... Medicine and Pharmacy, Bucharest, Romania ALINA D ZAMFIR • Department of Chemical and Biological Sciences, “Aurel Vlaicu” University of Arad, Arad, Romania; Mass Spectrometry Laboratory, National Institute for Research and Development in Electrochemistry and Condensed Matter, Timisoara, Romania DIETER R ZIMMERMANN • Institute of Surgical Pathology, University Hospital Zurich, Zurich, Switzerland PART I PROTEOGLYCANS... (and RNase A to be added to P1), P2, P3, QBT, QC, and QF; QIAfilter cartridges; and QIAGEN-tips (500 size) – Isopropanol was from Fisher – TE buffer: 10 mM Tris–HCl/1 mM EDTA (pH 8) Tris solution is made from Tris base in ddH2O and pH is adjusted with HCl EDTA is added from 0.5 M stock solution 2.12 Digestion of Plasmid Constructs with XbaI XbaI, 10× NEBuffer 4, and 10 mg/mL BSA were from New England... PN, PE, and EB, and QIAquick spin columns 2.16 Ligation to Produce Full-Length cDNA T4 DNA ligase and 10× T4 ligase reaction buffer were from New England Biolabs 10 M Cortes et al 2.17 Transformation into Competent Cells and Small-Scale Plasmid DNA Preparations The materials used were as described in Subheadings 2.7–2.9 2.18 Screening for Correct Full-Length cDNA Constructs BamHI, NEBuffer 3, and 10 . Zurich , Zurich , Switzerland PART I PROTEOGLYCANS sdfsdf 3 Françoise Rédini (ed.), Proteoglycans: Methods and Protocols, Methods in Molecular Biology,. Effects of Proteoglycans on Proteinase Activities . . . . . . . . . . . . . 307 Steven Georges, Dominique Heymann, and Marc Padrines 21 Proteoglycans and Osteolysis.