cardiomyocyte apoptosis in heart development methods and protocols

... National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland Craig Monger  •  National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland; National Institute ... Bioprocessing Research and Training (NIBRT), Dublin, Ireland Sara Carillo  •  National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland Lesley M. Chiverton  •  Industrial ... National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland Martin Clynes  •  National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland Orla Coleman 

Ngày tải lên: 14/05/2017, 11:19

... dendritic cells This interaction causes an increase in pro-infl ammatory cytokines and chemokines The increase in infl ammation eventually leads to edema, erythema, abscess formation, and mucosal hemorrhages ... establish clean point mutations and in-frame deletion mutations that have been instrumental in identifying and understanding the molecular interactions between components of the Yersinia type III ... synthesis and secretion translocators have been studied in some detail Utilizing in-frame deletion mutagenesis uncovered two LcrH-interacting domains in YopD—the fi rst being a large N-terminal domain

Ngày tải lên: 25/12/2016, 15:05

... as insulin-like growth factor-binding proteins (IGFBPs) [ 23 ] and interleukin-6 (IL-6) (reviewed in ref 24 ) These observations indicate that senescence is not just dictated by events happening ... gelatin and let coverslips dry before cell plating 7 Cells should always be plated in duplicate for experimental replicates 8 SA-βgal staining should be performed in the evening Staining ... oncogene-induced senescence in 1997 by Serrano et al., many outstanding researchers have been working on this intriguing set of phenotypes In addi-tion to proliferation arrest, cells undergoing oncogene-induced

Ngày tải lên: 25/12/2016, 15:07

... and 2 (HTLV-1 and HTLV-2) infections in HTLV seroindeterminate individuals from Sao Paulo, Brazil J Clin Virol 44(3):185–189 80 Garin B, Gosselin S, de The G, Gessain A (1994) HTLV-I/II infection ... HTLV indeterminate pattern provisionally called “New HTLV indeterminate” indeterminate pattern (T–Z) HTLV-1 Infection Epidemiology and Diagnosis Trang 25discriminate between HTLV-1 and HTLV-2 infections ... protein (GD21) of HTLV-1 and HTLV-2 as well as specific synthetic peptides originating from HTLV-1 (including MTA-I or rgp46-I) and HTLV-2 (including K55 or recognize both HTLV-1 and HTLV-2 infection

Ngày tải lên: 13/05/2017, 21:19

... Cholesterol-Binding Domains in Membrane Proteins Coralie Di Scala and Jacques Fantini Abstract In eukaryotic cells, cholesterol is an important regulator of a broad range of membrane proteins, including ... cholesterol complexing agents partition into mem-branes and sequester cholesterol These agents include digitonin, filipin, nystatin, and amphotericin B Digitonin is a plant glycoal-kaloid saponin detergent ... ligands (oxysterols and certain intermediates in cholesterol synthesis) LXR upregulates a suite of genes, including those encoding two proteins that export cholesterol from the cell The ATP-binding

Ngày tải lên: 13/05/2017, 21:19

... control sample in which no polarizing cytokines (rh TGF-β and rh IL-4) are added, may be included in the protocol for a baseline determination of IL-9 Vitamin D is an inhibitor of cyclins and treatment ... Diseases in Research and Clinical Settings Patricia Keating and James X. Hartmann Abstract Th9 cells are associated with atopic and inflammatory diseases, and their increased levels and function ... Cells: Methods and Protocols, Methods in Molecular Biology, vol 1585, DOI 10.1007/978-1-4939-6877-0, © Springer Science+Business Media LLC 2017 257 Th9 Cells: Methods and Protocols 258  Index

Ngày tải lên: 13/05/2017, 21:20

... possession an ever-expanding arsenal that continues to aid us in dissecting the function of telomerase and telomere-binding proteins, probing the changes in telomeres, and elucidating the consequences ... transfer and minimize bubbles 9 The membrane may be further incubated in NaOH solution for 5–10 min to denature any remaining DNA, neutralized again, and rinsed in 2× SSC before cross-linking 10 ... (2001) The Pin2/TRF1- interacting protein PinX1 is a potent telomer- ase inhibitor Cell 107:347–359 49 Baumann P, Cech TR (2001) Pot1, the tive telomere end-binding protein in fission yeast and humans

Ngày tải lên: 13/05/2017, 21:21

... parental origin The imprints are established early in development and, once set, the imprint persists in multiple tissue types over decades.There is evidence that some imprinting methylation in humans ... of interest 2 Hongseok Ha et al. Trang 13For studies involving multiple samples, Illumina provides6 bp index sequences for pooling multiple samples in one ing library We tested 48 indexes and ... blocking during runs 16 If tips are to be used again within 24 h they can remain in thetip holder and be stored in the fridge, inside the black box.Ensure that there are some volumes of NDTs remaining

Ngày tải lên: 13/05/2017, 21:21

... quantitative information on immune cell migration and interactions including the velocity, meandering index, turning angles, duration and timing of interactions, and physiological outcomes of these interactions ... insights into mechanisms regulating immune cell migration includ-ing entry and exit from tissues and migration within those tissues Increasingly it has become clear that modifying the kinetics ... University of Birmingham Birmingham, UK Trang 5Welcome to the second edition of Methods and Protocols for assessing T cell Trafficking, in the Methods in Molecular Biology series The trafficking of T

Ngày tải lên: 13/05/2017, 21:21

... Medicine at Mount Sinai, New York, NY, USA; Immunology Institute and The Mindich Child Health and Development Institute, Mount Sinai School of Medicine, New York, NY, USA elena MolIna • Instituto ... chromatographic methods in protein purification Part II, Food Allergen Discovery, Detection, and Quantification, can be classified into three types of methods including DNA-based methods, protein-based methods ... immunodetection and providing certain discriminating character-istics such as isoelectric points or molecular mass Using 2DGE, proteins are fractionated according to their isoelectric points in the first

Ngày tải lên: 13/05/2017, 21:22

... of four zinc fingers and a low- complexity activation domain, both of which are required for pro-moting DNA binding and mediating transcriptional activation During ZGA, Vfl/Zld-binding sites ... novel binding sites using structural information RBPmap [ 58 ] Scan for known RNA-binding motifs RNAcontext [ 59 ] Discovery of novel binding sites using structural information MEMERIS [ 60 ] Incorporates ... slows down from 10 min in NC11 to 21 min in NC13 phase lengthens and by cycle 14 a difference between early and late replicating euchromatin and the satellite DNA becomes obvious Interphase 14 corresponds

Ngày tải lên: 13/05/2017, 21:24

... Protein-Ligand Binding Sites and Structures 97Hui Sun Lee and Wonpil Im 9 Local Alignment of Ligand Binding Sites in Proteins for Polypharmacology and Drug Repositioning 109Michal Brylinski ... query protein structure,including global and local structure matching to known proteins Chapter8describes G-LoSA, which finds ligand binding sites similar to a query binding site within a referencedatabase ... following chapter (Chapter9), aligns two ligand binding sites toquantify similarities between them In Chapter10, WATsite2.0 is introduced, which predictsbound water molecules in a ligand binding

Ngày tải lên: 13/05/2017, 21:25

... contain-ing ampicillin with an individual bacterial colony and incubate at 37 °C overnight in a shaker incubator (240 rpm) Purify the plasmid DNA using QIAprep Spin Miniprep Kit according to ... 10% fetal bovine serum (FBS; HyClone Laboratories, Inc.), 100 U/mL penicillin-100 μg/mL streptomycin, and 2 mM L-glutamine (Mediatech) (b) BHK-21 and RK-13 cells are maintained in Eagle’s mum ... directly into the tube containing TransIT-LT1/OPTI-MEM I and mix by pipetting gently or vortexing for 2 s Incubate mixture at RT for 30 min 5 Remove medium from tissue culture dish containing attached

Ngày tải lên: 16/05/2017, 23:21

... UPR and apoptosis in different organsand the development of diabetes ER stress, UPR and apoptosis in the pancreas b-cell loss plays a crucial role in the development of insulin deficiency and in ... impaired insulin action (insulin resistance) par-alleled by impaired insulin secretion and a progressive decline in b-cell function Insulin resistance, often associ-ated with obesity and physical inactivity, ... P58IPK(DNAJC3) and EIF2a, impair b cell health and function [4,25–28] Moreover, mutations in proinsulin causing disruption of disulfide bond pairing result in misfolding and accumulation of proinsulin in

Ngày tải lên: 02/11/2022, 09:29

... scattered light contains information, ing detection and analysis of the particles which is employed in common and new techniques based on light scattering allow-Bearing in mind the principle of energy ... Netherlands Trang 12Andrew F Hill (ed.), Exosomes and Microvesicles: Methods and Protocols, Methods in Molecular Biology, vol 1545, DOI 10.1007/978-1-4939-6728-5_1, © Springer Science+Business ... Elastic and Inelastic Light Scattering Trang 16Examples for elastic light scattering are Rayleigh scattering and Mie scattering, which will be explained below If the energy of the incident and

Ngày tải lên: 24/12/2016, 22:31

... Methods in Molecular Biology 1573 Brad M Binder G Eric Schaller Editors Ethylene Signaling Methods and Protocols Methods in Molecular Biology Series Editor John M Walker School of Life and ... and focuses on the analysis of the novel membrane-associated proteins involved in the initial perception and transduction of the ethylene signal, including the ethylene receptors, CTR1 and EIN2 ... 190 Brad M Binder and G Eric Schaller (eds.), Ethylene Signaling: Methods and Protocols, Methods in Molecular Biology, vol 1573, DOI 10.1007/978-1-4939-6854-1, © Springer Science+Business Media

Ngày tải lên: 13/05/2017, 21:17

... China yong Xu • Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI, USA Trang 12Oliver Schilling (ed.), Protein Terminal Profiling: Methods and Protocols, Methods ... of Protein N-Termini and N-Terminal Acetylation Quantitation 17 Willy V Bienvenut, Carmela Giglione, and Thierry Meinnel 4 Profiling of Protein N-Termini and Their Modifications in Complex ... further volumes: http://www.springer.com/series/7651 Trang 3Protein Terminal ProfilingMethods and Protocols Edited by Oliver Schilling Institute of Molecular Medicine and Cell Research, University

Ngày tải lên: 13/05/2017, 21:18

... understanding the development of recombinant viruses and their use as vaccine platforms The methods and protocols contained herein involve many of the viruses cur-rently being used for, or under development ... from infection by a wide variety of infectious agents This volume is intended for scientists and clinicians who are interested in learning more about and adapting methods employed in basic and ... Buffer 7 Mix by vortexing and incubate at 65 °C for 10 min inverting the tube (and incubate on ice for 20 min) Trang 29 11 Add 700 μl of absolute isopropanol and mix by inverting 12 Centrifuge

Ngày tải lên: 13/05/2017, 21:19

... first methods collection entitled Bacterial Toxins: Methods and Protocols was published which contained 20 chapters on protein toxins and endotoxin from bacteria and cyanobacteria Then, in 2011, ... Microbial Toxins: Methods and Protocols, Methods in Molecular Biology, vol 1600, DOI 10.1007/978-1-4939-6958-6, © Springer Science+Business Media LLC 2017 199 Microbial Toxins: Methods and Protocols ... inoculating loop into an Eppendorf tube containing 1 mL of 0.1 M citric acid aqueous solution (see Note 2) Mix the cell suspension by vortexing and incubate at 100 °C for 90 min After cooling

Ngày tải lên: 13/05/2017, 21:24

... using single-, dual-, or multi-stain techniques Single-staining methods using nucleic acid stains such as EB, PI, 7-AAD, DAPI, SYTOX Green, and SYTOX Red, and enzymatic stains such as CFDA and ... Oncology and Musculosceletal Diseases”, Branch of Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Würzburg, Germany; Chair Tissue Engineering and Regenerative Medicine, University ... chapter include MTT, WST, and resazurin They are applicable for adherent or suspended cell lines, easy to perform, and comparably economical Detailed protocols and notes for easier handling and avoiding

Ngày tải lên: 13/05/2017, 21:24