TC khoa hoc VNU vol 31 no 2s

Nghien ciru g6m 2 kh6i thf nghiem b6 sung ham hrong Zn va Pb nrong dirong voi mire 0 nhiem va rftt0 nhiem.. LAymiu thuc v~tathoi diSm 20 va 40 ngay de nghien ciru kha nang tfch lfiy Zn,

Hien nay tren th€ gioi d1lco mQt s6 nghien ciru v~ anh huang cua kim loai nang (KLN) d€n cay keo neo Theo Chadran va nnk (2009) [1], khi thuc v~t phat trien tren d~t 0 nhi€m KLN thl ham hrong di~p luc giam do tang tang ch~t r~n hoa tan, clorua, sunphat hoac nitrat Ben canh d6, di~p l\lc t6 bi giam cling c6 th~ la k€t qua cua sv phan huy mang l\lc ll;lPvOi kim lo~i du thua lam giam kha nang quang hqp cua laoTheo Kamarudzarnan va nnk (2005) [2], khi tr6ng cay keo neo d~ xu ly nu&c thai chua Fe va Mn theo cong ngM dong chay ngang c6 th~ lo~ bo 91,5 - 99,2% va 94,7 - 99,8% Fe va Mn trong

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• Tac gia lien h~ DT : 84-912733285

Email: tranthituyetthu@hus edu.vn

Cay keo neo (Limnocharis Flava) co nguon

g6cavung nhiet dOi va c~n nhiet dOi CMu My

oVi~t Nam cay moe hoang dai ho~c trong chu

y€utai vimg d~t ng~p mroc khu VI,lC d6ng b~ng

song Ciru Long Phan than hi non thuCmgdUQ"c

su d\mg U~mrau an va la m6n rau d~c SilO

khong th~ thi€u trong m6n Iftuca keo cua nguOi

danNamBQ

1 D,t vin d~

Tom tit:Cay keo neo(Limnocharis Flava)130 thuc v~t sieu tfch liiy kim loai ~ng Cay thi nghiem

(5 tu~n tu6i , trung blnh 4-5 la, chieu cao 10,93 em) duoc tr6ng trong dit gay 0 nhi~m Zn, Pb a

ding ham hrong 80 va 240 ppm Ket qua sau 10 , 20,30,40 ngay chua gay anh huang b~t 19i den

chieu cao, di~p luc t6ng s6 rna con kfch thfch cay phat trien Trong d~t b6 sung Zn,athoi diem 40

ngay , chien cao cay cao nh~taen.2 (240 ppm) 25,98 cm va th~p nh~taCfO (d6i chimg) 23,92

em; ham luong di~p luc t6ng s6Iannh~taCf1.2 0 , 043 rag/em ' va th~p nh~taCfO 0,039 mg/cnr '

Nguoc lai, ad~t b6 sung Pb dli gay nhieu b~t 19i, cu the la chieu cao va diep luc t6ng s6 giam

manh sau 30 - 40 ngay Trong d6, thoi diem 40 ngay, chieu cao cay cao nh~t aCTO (d6i chimg)

23,92 em va th~p nh~taCf 2 2 (240 ppm) 18,99 em , ham luong di~p luc t6ng s61an nh~taCfO

0,039 mg/cm 2 va th~p nh~taCf2 2 0,Q3 mg/cm", Su tfch liiy Zn, Pb trong sinh kh6itYl~ thuan

voi hrong b6 sung va ph~n r~ nhieu han than la o Thoi diem 40 ngay, t6ng luong tfch liiy Zn cao

nhatUCT1.2 415,7 ppm va th~p nh~tUCTO 145,46 ppm, Pb cao nh~taCf2.2 8 , 27 ppm v a th~p

nh~t aCTO 2, 66 ppm

Tit khoa: Kim loai n~ng,kern,chi, cay keo neo, Lirnnocharis Flava.

Nh~ ngay 15 thang 6 nam 2015

Chinh sua ngay 16 thang 7 nam 2015; Chip nh~ dang ngly 21",1 .s

Tr§n Thi Tuyet Thu1,*, Nguyen Vi~t Hi~p2, Duong Thanh Van1

2Vi~nTh6nhuimg Nang hoa , Bong Ngac , Tit Liem; Ha Nvi, V~ Nam

(Limnocharis Flava) a giai doan cay non

T\ll) chi KhoahQCDHQGHN:KhoahQCTVnhien va Cong ngh~,T~p31,sA2S (2015)274-280

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3 1 Anh hU07zgcua Zn din c{iy keo neo

3 1.1 Anh hU07zgcua Zn din chiiu cao cay

K6t qua nghien ciru anh huang eua Zn d~n ehi€u eao cay keo neo dUQ'etrinh bay abang 1 eho thAya cac cong thuc thi nghi~m (CTTN)

3 K~tqua nghien cUu

N, P,K t6ng s6 va d~ tieu giau, ham hrong Zn

va Pb t6ng s6 97,23 ppm va 21,13 ppm, thftp han so voi quy chuan cho phep QCVN 03:2008IBTNMT

Can cir vao mQt s6 kat qua nghien ciru tren th~ gioi va QCVN 03:2008IBTNMT dS gay 0 nhiem dfrt Nghien ciru g6m 2 kh6i thf nghiem b6 sung ham hrong Zn va Pb nrong dirong voi mire 0 nhiem va rftt0 nhiem M6i thf nghiem g6m 3 cong thirc (CT), m6i CT diroc I~p I~ 3 Iftn, trong d6 CT d6i chirng duoc b6 trf chung cho 2 kh6i thf nghiem, Thi nghiem 1: Nghien ciru anh huang ella Zn d~n cay keo neo, bao g6m 3 cong thirc (1) BBi chirng CTO: khong b6 sung Zn, (2) CT 1.1: bB sung 80 ppm Zn, (3)

CT 1.2: b6 sung 240 ppm Zn Thi nghiem 2: Nghien ciru anh huang cua Pb d~n cay keo neo, bao g6m 3 cong tlnrc (1) eTO: tr6ng 3 cay keo neo, khong b6 sung Pb, (2)CT 2.1: b6 sung 80 ppm Pb, CT 2.2: bB sung 240 ppm Pb

Tian hanh do chieu cao cay, d~m s6 la, do diep luc trong la tai thoi di€m 10,20, 30, 40 ngay d~ nghien ciru anh huang ella Zn, Pb d6n sir phat triSn cua cay LAymiu thuc v~tathoi diSm 20 va 40 ngay de nghien ciru kha nang tfch lfiy Zn, Pb trong cac b{l ph~n than la, &

PM miu dAt, thl,lc v~t theo phuang pMp chuftn

va xac dinh Zn, Pb trong dung dich pM miu b~ng may quang phB hip ph1,lnguyen tir AAS

Sir d1,lngphan m€m Excel xir19th6ng ke va rno

ta sBli~u

BAtthf nghi~m la dftt phil sa song H6ng

duQ'cb6i hang nam, thu~n IQ'icho S\l pMt tri€n

cua cay keo neo BAt c6 phin ting gftn trung

tinh (pH = 6,8), chftt hfru co trung binh cao

(OM 2,85%), CEC trung blnh (12 meq/l00g

dAt), thanh phftn co gi6i trung blnh, ham IUQ'llg

NQi dung nghien elm: (1) Anh huang cua

Zn d~n cay keo neo, (2)Anhhuang cua Pb d~n

caykeo neo

Cay keo neo duoc nhan gi6ng b~ng hinh

thirc sinh san vo tfnh,dt cay con moe tren cay

m~a thOi diSm 3 tuftn tu6i Tr6ng 3 cay con

vao m6i ch~uthf nghi~m (kich thuae 30 x 30 x

40 cm, chua 5 kg dAtkho da d6 ng~p 5 cm nuac

cAtdS 6n dinh di€u ki~n moi trmmg va ph1,lch6i

ho~t tinh sinh hQCdAt trong mQt tuftn).Sau 2

tu~n tr6ng cay phat tri€n khoe m~nh, chi€u eao

trung blnh la 10,93 cm, s6 la tuang d6ng nhau

(4 - 5) la, bQr~ bam dAt6n dinh thl b6 sung Zn,

Pb

2 Nqi dung, dai tll(}'Dgva phuong phap

nghien cUu

mroc rirae aMalaysia Con theo Abhilash va

nnk (2009) [3], caykeo neoa An BQ e6 th€loai

93 - 95% Cd trong dAt0 nhi~m 0,5 - 4 ppm Cd

12 tuan tr6ng keo neo tren dAt ng~p nuoc 0

nhiem KLN ciing da chi ra sir tfch liiy Fe, Cu,

Zn, Pb, Hg tang d~n theo thoi gian, hieu qua

tich lily 20 - 77% va t~p trung chu y~u trong r~

>than hi. Trong d6, Fe tfch liiy nhieu nhAta

phan r~ 456 - 1549 ppm va Pb 1,2-7,6 ppm

B~n nay Vi~t Nam chua e6 cong b6 v€ anh

huang cua KLN d~n cay keo neo, VI v~y,

nghien ciru nay da dua ra co sa khoa h9C

khuyen cao viec tr6ng keo neo tren dAt0 nhi~m

KLN dS sir dung lam rau an, d6ng thoi c6 th€

sir dung cay keo neo xir19dAt0 nhiem

T.T.T Thuvannk: ITgp chi Khoa h9CDHQGHN: Khoa h9C TITnhienvaC6ng ngh¢ Tdp 31.sa2S (2015) 274-280 275

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CITN TruaeTN 10 ngay 20 ngay 30 ngay 40 ngay CfO 0,023 0,025 0,029 0,033 0,039

±0,05 ±0,06 ±0,01 ±O,OI ±O,QI Cfl.l 0,019 0,016 0,027 0,036 0,042

±0,09 ±0,08 ± 0,Dl ± 0,Dl ±0,02 Cf1.2 0,022 0,02 0,033 0,04 0,043

±0,02 ±Om ±0,02 ±0,o2 ±O,Ol

3.1.3 Anh huang cua Zn din ham tU'971g di?p luc tang sa trong ld keo neo

Ham hrong diepluc t6ng s6 co vai tro quy~t dinh den kha nang quang hop cua Ia cay, VI v~y

se co vaitroquan trong d~n SlJ phat tri~n cua cay.

Bang 3.Armhuong ella Zn d€n tAngdiep luc trong la keo nco ( mg/cnr')

th~p nhAt&CTO la 5Ia va cao nh~t &CT1.2Ia 6 Ia.

Khi so sanh s6 hi&cac CTTN trong cung I

khoang thoi gian, k~t qua nh~n thAy&thoi di~m

10 ngay b6 sung Zn vao dAt, s6 Ia giam d~n tU

CTO d~n CT1.2 trong do thAp nhAt la CT1.2 con

3 hi Thoi di~m 20 va 40 ngay, s6Ia keo neo co

xu huang tang len & cac cong thirc Sau khi b6

sung Zn diroc 40 ngay thl cay keo neo co s6 Ia

Bang 2 Anh huong ella Zn d€n sf, hi cay CTTN TruaeTN 10 nga~ 20 nga~ 30 nga~ 40 nga~

CfO 4-5 4-5 5 4-5 5

Cf1.2 4-5 3 4-5 5 6

S6 la la mQt trong nhirng chi tieu quan trong d~ daub gia kha nang sinh tnrong cua cay S6 Ia tao dieu kien thuan IQ'icho qua trinhquang hop

va trao d6i chAt t6t han K€t qua anh hirong cua

Znd~n s6 la cua cay keo neo duoc trmh bay &

bang 2 nhu sau:

o thoi di~m 10 ngay, mire tang chieu cao

CT1.2 , tuy nhien sau 20 ngay lai co xu huang

giam din & CTOva tang len & CTl.1 va CT1.2.

Sau 40 ngay , chien cao cay cao nhAt&CT1.21a

25 , 98 em, thAp nhAt & CTO la 23,92 cm Chirng

to trong pham vi nghien ciru, Zn la nguyen t6 vi

hrong co kha nang thtic dfty SlJ tang tnrong, lam

tang chieu cao cua cay.

17,51±2,07 16,11±2,08

16 , 55±l,93

23,92±2,98 24,88±2,01 25,98±2,65

20,78±2,4 20,48±2 , 09 21,40±2,44

15,14±2,06 12,90±2,51 12,56±1,04

10,93±1,73 1O,93±l,73 lO,93±1,73

CfO Cf1.l CT1.2

30 ng!r

20ngay

10 ng!r 40 ng!r TruaeTN

CITN

chieu cao cay co xu huang tang nhanh & CTl.I

vaCT1.2, thAp nhAt CTO.

khac nhau chiSu cao city dSu tang len tai m6i

thoi di€m do (10 ngayll~n) Sau 10 ngay, chieu

cao cay &cong thirc CTO, CTl.1 va CT1.2 tang

I~nhrot 138%, 119% va 115% so voi thoidi~m

Bang 1.Armhuong cua Zn d€n chieu eao cay keo nco (em)

276 T T.T Thuvannk: /Tap chi Khoa hoc DHQGHN: Khoa hoc Til nhienvaCong ngh~, T~p 31, S6 2S (2015) 274-280 I

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-Bang 5 AMhuemg cua Pb dan chi6u cao cay keo neo (em) CTNT TruaeTN 10 ngay 20 ngay 30ngay 40 ngay

CTO 10,93 ± 1,73 14,14 ± 1,24 17,5 ± 1,45 20,28 ± 3,2 23,92± 2,03

CT2 1 10,93 ± 1,73 13,77 ± 2,2 16,62 ± 2,25 19,57 ± 1,56 22,52± 3,02

CT2.2 10,93 ± 1,73 12,73 ± 1,74 14,64 ± 2,03 16,65 ± 2,31 18,99 ± 2,09

3.2 Anh huang da Ph tI~ncay keo neo 3.2.1 Anh hutmg cua Ph tI~nchi~u cao cay

chien cao cua cay keo neo duoc trinh bay trong

ngay/lAn) chieu cao cay keo neo cao nhAt (] cong tlnrc d6i clnrng, thApnhAt (] cong thirc b6 sung 240 ppm Pb Cu th€ la chi~u cao trung

Trong thoi gian 20 dan 40 ngay, ham luong

Di~u nay dii cho th~y Zn la nguyen t6 dn thiet

cho su phat triSn cua cay, thea thoi gian cay dn

dinh dufrng d€ phat tri€n vi v~y rna ham luong

neo (] cong thirc CTl.1Ia 289,82 ppm (gAp 1,99

IAnd6i clnrng) va CT1.2Ia 415,7 ppm (g~p 2,86

IAnd6i chUng) Nhuu v~y vo; IUQ'IlgZn b6 sung

cang cao thi kha nang tich liiy vao cay cang 160

Bang 4 Tfch liiy Zn trong cay keo neo (ppm)

CTIN 20 ngay 40 ngay

Than Ili Re Thanlare Thanla Re Thanla re

CTO 66,16 91,24 157,48 55,54 89,92 145,46 CTl.1 99,36 186,36 285,72 99,3 190,52 289,82 CT1.2 115,6 270,12 385,72 113,56 302,14 415,7

Co thS nh~ th~y tai thoi diSm nay, ham luong

voi ham hrong Zn duqc b6 sung vao dfrt

K~t qua nghien CUu anh huang cua Zn d6n

su tfch liiy Zn trong than Ia va r€ cay keo neo (]

thoi diSm 20 ngay ham hrong Zn tfch lily (] phAnr€ cua cay (] cac cong thirc CTO;CTl.1 va

diSm 40 ngay cao g~p 1,62; 1,92 va 2,66 IAnso

K6t qua nghien cuu duoc trinh bay (] bang 3

cho th~y sau 10 ngay thf nghiem, ham luong

diep h.IC t6ng s6 trong hi keo neo (] CTl.1,

CT1.2 co xu huong giam so voi thoi diSm tnroc

thf nghiem, giam nhanh nh~t (] CTl.1 (giam

15,7%), tuy nhien aCTO tang 109% so voi thoi

diSm tnnrc thf nghiem

Ham hrong diep h,IC t6ng s6 (] thoi diSm til

20 d6n 40 ngay, co xu huong tang (] ca 3 cong

thirc, tang nhanh nh~taCTl.l (b6 sung 80 ppm

Zn); thoi diSm40 ngay, dao dQng 0,039 - 0,043

mg/cnr', trong do th~p nh~t (] CTO la 0,039

mg/cnr' va 160 nh~t (] CT1.2 la 0,043 mg/cm'

T.T.T Thuvannk: IT(lp chi Khoa hoc DHQGHN: Khoa hoc T7!nhienvaCtmg ngh~, Tdp 31,sa2S (2015) 274-280 277

thirc, tang nhanh nhAtaCTO T~ thai di~m 40 ngay, thAp nhAtaCT2.2 la 0,03 mg/crrr' va Ian nhAta CTO la 0,039 mg/cm' Ket qua nghien CUunay phil hop voi nghien CUucua Chadran

va nnk (2009) khi nghien cUu b6 sung ham

IUQllgPb til 10 dan 100 ppm vao dAtthi nghi~m

va ti~n hanh thea doi trong thm gian 25 ngay [1]

othai di~m 10 ngay thi nghiem, ham hrong

diep luc t6ng s6 trong Ia cay keo neoa CT2.1,

CT2.2 co xu huang giam so voi truce thi

nghiem, giam nhanh nhAtaCT2.2 (giam 30%)

Trong khi do,acong thirc CTOchi tangit ,b~ng

109% so v6'itruac thi nghi~m.Thai di~m til 20

den 40 ngay, co xu huang tang a ca 3 cong

CfO 0,025 ±0,06 0,029 ±om 0,03 ± 0,Dl 0,039 ±0 , 01 CT2.1 0,02 ±0,02 0,027 ± 0 , 01 0,03 ± 0,Ql 0,037 ± 0,01 CT2.2 0,018 ± 0,02 0,Q3 ± 0,04 0,Q3 ±O,04 0,Q3 ± 0,Q2

yang va rung la, lam giam s8 la keo neo acac CTTN so voi d6i chimg

3.2.3 Anh huang eua Ph din ham luang di~p luc tang sa trong lei keo neo

Ket qua duoc trinh bay trong bang 7 cho thAy c6 m6i nrong quan tY I~ nghich gifra ham hrong hrong diep luc t6ng s6 trong la cay keo neo voi ham luong Pb duoc b6 sung vao dAta

cac CTTN so voi d6i chimg

Bang 7.AM huang cuaPb d~n t6ng diep h,IC trong hi keo neo ( mg/cnr')

D6i voi cong thirc CTO khong b6 sung Pb,

cay coSl,lphat trien d~u den thai di~m 40 ngay,

trung blnh 5 -6 la, m6i cay tang 1- 2 Ia so voi

cac CTTN D6i voi CT2.1, CT2.2 chiu anh

huang cua 0 nhi~m Pb co s6 hi giam, giam ro

nhAta CT2.2 b6 sung 240 ppm Pb (Bang 6)

Chirng to dng Pb dll anh huang den qua trinh

sinh 1:9sinh hoa trong cay nen co hien nrong Ia

Bang 6.AM huang cua Pb d~ns6hi cay(cm) CITN TnracTN 10 ngar 20 ngar 30 ngar 40 ngar

tnrong cua cay keo neo thong qua lam giam chi~u cao cay

3.2.2 Anh huang eua Ph din sau cuacay

Ket qua nghien ciru duoc trinh bay trong bang 6 cho thAyanh huang cua ham hrong cua

Pb trong dAt va s6 la cua cay keo neo tY I~

nghich voi nhau, thea thai gian, bam hrong Pb trong dAtcang cao thl s6 hrong la tren cay cang giam,

SI,l thay d6i chieu cao cua cay cling co su

nrong d6ng voi ham hrong Pb trong dk dgiai

doan 10 ngay, chien cao cay dat giatricao nhAt

aCTO 13014,14 em va giam d~naCTI.1, CT2.2

va thAp nhAta CT2.2 13012,73 em Tuy nhien

sau thai di~m 20 ngay, mire dQ tang chieu cao

cay dll tang thea nrng cong thirc thi nghiem va

tang Ian nhAtaCTO. othai di~m 40 ngay chien

cao cay Ian nhAta CTO la 23,92 em va thAp

nhAtCT2.2 la 18,99 cm.Nhu v~y trong pham vi

nghien ciru nay, Pb dll anh huang den su tang

[1] Chadran S and et al (2009), Studies on the Ecology, Distribution and Unilitarian aspects of Limnocharis flava Bunchenau, an invasive aquatic weed in Kuttand Wetland Ecosystem, School of Inviromental Sciences Mahatma Gandhi University, Mahatma Gandhi University, Priyadharshini Hills P.O., Kottayam- 686 008, Kerala, India.

[2] Kamarudzaman A N., Aziz R A., and Jalil M.

F (2005), Removal of Heavy Metals from landfill leachate Using Horizontal and Vertical Subsurface Flow Constructed Wetland Planted with Limnocharis flava International Journal of Civil & Enviromental Engineering, Volume 3,

No 2.

[3] Abhilash P. c Pandey V C., Srivastav P., Rakesh P S., Chandran S., Singh N., Thomas A.

P (2009), Phytofiltration of cadmium from water by Limnocharis flava Buchenau grown in free-floating culture system, Journal of Hazardous Materials, pp 791 - 797.

[4] Anning A K., Korsah P E and Fordjour P.A (2013), Phytoremediation of wastewater with Limnocharis flava, Thalia geniculate and typha latifolia in Constructed wetlands, International Journal of Phytoremediation, pp 452-464.

Tal li~u tham khao

cang Ion, con (1ham hrong Zn duoc b6 sung trong thf nghiem chua co d~u hieu gay tac d9ng bAtloi dan qua trlnh phat tri~n cua cay keo neo

Su tich liiyim, Pb trong r€ nhi~u hon than la va

co quan M tY l~ thuan voi bam hrong Zn, Pb dtroc b6 sung vao dit

2,66 4,76 8,27

Than hi,r8

Than hi R~

40n~

Nghien CUu tr6ng cay keo neo trong dAtgay

onhiem 80 ppm va 240 ppm Zn chua gay anh

huang b~t 19i dan suphat tri~n cua cay rna con

anh huang tich C\lC dan tang chieu cao cay, s6

la, ham luong diep h,IC t6ng s6 trong laoNhtrng

ciing (1dAt co cung ham luong Pb 80 ppm va

240 ppm da gay anh huang bit 19i dan chieu

cao cay, s6 la, ham luong di~ph.IC t6ng s6 trong

la keo neo

Khi ham hrong Pb diroc b6 sung vao dit

cang cao thl gay anh huang tieu cho cay

4.K~tlu,n

o cong thirc CTO khong gay nhi€m Pb co

ham hrong Pb tfch lily trong than va r€ tang nhe

trong thoi gian 20 d~n 40 ngay.othoi di~m 40

ngay, t6ng ham hrong Pb tfch Illy trong than la

r€ tai CT2.11a 4,76 ppm va CT2.21a 8,27 ppm,

cao hon so vci d6i chimg 2,1 ppm (gAp 1,79

Ian) va 5,61 ppm (gAp 3,11 Ian) K~t qua nay

chirng to r~ng khi tr6ng cay keo neo(1giai doan

cay non tren dAt0 nhi€m KLN, nau dung lam

rau an co th~ lam tang nguy co gay tfch lfiy dQc

chAt vao cay, ti~m frnnhirng rui ro d~n sire khoe

con ngiroi Tuy nhien, dirng (1 phirong dien

dung thuc v~t d~ xu ly dAt0 nhi€m KLN thi keo

neo nen duoc xem la d6i nrong dn quan tam

nghien ciru

CTNf 20 nga~

Thanh! R~ Than hi,rS

gian va thea S\l tang ham hrong Pb trong cac CTIN, hrong Pb tfch lfiy trong r~ Ian hon trong than la (Bang 8)

3.2.4 S" ticb Lily Ph trong cay keo neo

K~t qua nghien ciru (1thoi di~m 20 va 40

ngay gay nhi~m Pb cho thAyt6ng ham luong Pb

tich h1y trong cay keo neo tang dan thea thoi

Bang 8 Tfch liiy Pb trong cay keo neo (ppm)

T.T.T Thu vi! nnk IT(lp chi Khoa hoc DHQGHN: Khoa hoc Til nhien vi! Cong ngh?, Tdp 31,S62S (2015) 274-280 279

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Abstract: Limnocharis flava is considered as one of the heavy metal hyperaccumulating plants.It

is wild or planted in the Mekong river delta and serves as a cooking ingredient for goby soup

Seedlings (5 weeks old, 4 - 5 leaves, 10.93 em in height) were planted on soil in which contents of Zn

and Pb were 80 and 240 ppm (respectively) and havested after 40 days Observations at 10, 20, 30, 40

days showed a positive effect of Zn on plant growth (height and chlorophyll contents) at its both

contents of 80 and 240 ppm After 40 days, the heights of plants varied from 23.92 em (CfO) up to

25.98 em (in Cf1.2) Chlorophyll contents of the CTO and Cf1.2 were 0.039 and 0.043 mg/crrr',

respectively Incontrast, Pb at the same content as Zn showed negative effects on plant growth After

30-40 days, the presence of Pb resulted in remarkly decreases of the height and chlorophyll contents

The heights of plants changed from 18.99 em (CT2.2) to 23.92 ern (CfO) The lowest chlorophyll

content was 0.03 mg/cm" (Cf2.2), whereas and the highest one was 0.039 mg/cm" (CTO) Zn and Pb

accumulated in the plant tisues showed a high correlation with the additive amounts, and they tend to

translocate in roots rather than leaves and shoots The highest amounts of Pb and Zn were 415.7 and

8.27 ppm, while the lowest values were 145.46 and 8.27 ppm, respectively

Keywords : Heavy metal, accumulation, zinc, lead, Limnocharis flava

Trftn Thi Tuyet Thu1,Nguyen Vi~t Hi~p2, Duong Thanh Van1

'Faculty 0['Environmental Sciences, VNU University of Science, 334 Nguyin Triii, Hanoi, Vietnam

Soils and Fertilizers Research Institute, Dong Ngac, Tit Liem, Hanoi, Vietnam

Accumulations of Heavy metals (Pb, Zn) and their influence

on Limnocharis Flava During the Nurseling Stage

280 T T.T Thu va nnk /T~p chi Klwa hoc DHQGHN : Klwa h9C tv nhien va Cong nghj , Tq.p31,sa2S (2015) 274-280