x ray and y ray detectors

... carboxyl terminal phosphorylation site (DDEASTTVSKTETSQVAPA) Peptide synthesis, for both the unphosphorylated and fully phosphorylated versions, has been described previously [7,16] The lyophilized ... evaluations and display for synchrotron radiation experiments: hardware and software Nucl Instrum Methods A249, 399–407 21 Guinier, A & Fournet, G (1955) Small Angle Scattering of X- rays Wiley, New York ... ð7Þ where fM and fD are the mass fractions of monomer and dimer, respectively, and RgM and RgD are the radii of gyration for the monomer and dimer, respectively Missing pieces of crystallographic...

Ngày tải lên: 22/02/2014, 07:20

... 4-(2¢,4¢-dimethoxyphenyl-Fmoc-amino methyl)phenoxyl-resins and Fmocamino acid derivatives by an automatic peptide synthesizer (Applied Biosystems, Foster City, CA, USA) At the end of synthesis cycles, ... and inhibitory activity of more than 10 chemically synthesized inhibitor analogues for TM-3 MATERIALS AND METHODS Materials 4-(2¢,4¢-Dimethoxyphenyl-Fmoc-amino methyl)phenoxylresins and Fmoc-amino ... superfamily of metzincin which exhibits some typical structural features, such as the Met-turn and active-site consensus HExxHxxGxxH sequence [15–17] Some organisms and mammalian tissues recently have...

Ngày tải lên: 08/03/2014, 23:20

... [29] SAL affinity for 1-amino-anthracene, for androstenone, and for a group of odors such as 3,7-dimethyl-1-octanol, 2-phenyl ethanol, 2-isobutyl-3-methoxypyrazine has been assayed by fluorescence ... 5a-androst-16-ene-3a-ol failed The wall of the cavity is mostly formed by hydrophobic residues: Val45, Phe58, Ala73, Val85, Phe93, Leu106 and Leu108 Three weakly polar residues, Tyr87, Tyr123 and ... spectroscopy, namely 5a-androst-16-ene-3-one or 5a-androst-16-ene-3a-ol, both known as sex pheromones for the pig [2] Surprisingly, this cavity is devoid of such ligands, but a smaller electron density...

Ngày tải lên: 17/03/2014, 17:20

... the study of a stable polymorph of paclitaxel,9 and the study of three polymorphs of sibenadet hydrochloride.10 DSC and XRPD have typically been used as separate techniques to study the polymorphism ... anhydrous and melts at approximately 220 °C Form III crystallizes as a hexahydrate, which reversibly converts to the monohydrate Form III and then to an anhydrous Form III above 120 °C Anhydrous ... simultaneous, and dynamic differential scanning calorimetry /X- ray powder diffraction (DSC/XRPD); and high performance liquid chromatography (HPLC) A total of 10 crystalline polymorphs were identified, six...

Ngày tải lên: 14/02/2014, 03:20

... nPS using X- ray crystallography, as described below A Solution and refinement of the structure using X- ray crystallography B The crystal structure of the pantoate–nPS complex was solved by molecular ... biosynthesis, and functions in fatty acid synthesis and energy metabolism [2] The structure and function of PSs from several prokaryotic and eukaryotic organisms have been reviewed recently [2] ... ring of ATP The carbon and oxygen atoms of pantoate are shown in yellow and red, respectively The carbon and oxygen atoms of ATP are shown in red, the nitrogen atoms in blue, and the phosphorus atoms...

Ngày tải lên: 16/02/2014, 09:20

... the wild-type protein in Fig 1B Asn29 and Lys29 are located in the bB strand and are partially exposed to the solvent by 20 and 39%, respectively In the wild-type protein, Asn29 forms hydrogen ... psychrotrophic RNase HI region of the aIII helix and a long loop between the aIII and aIV helices, respectively In the vicinity of Lys90, Lys89 is located Lys89, Lys90 and Arg97 are well exposed ... residues 29, T34 and E131 are shown The hydrogen bonds between N29 and Oc and T34 and Oc, and between N29 and Nd and E131 and Oe2, in the wild-type protein are shown as green broken lines, and the ion...

Ngày tải lên: 18/02/2014, 13:20

... recognize and select their substrates, we used X- ray crystallography, NMR and computational chemistry approaches to identify the molecular determinants of ligand specificity of CtCBM11 By means ... affinity and ligand specificity of type B glycan chain binders [5,8,11–13] Additionally, it has been shown that the specificity of CtCBM11 is consistent with the type of substrates that are hydrolyzed ... glucose monomers, and also by several van de Waals contacts that are promoted by the aliphatic side chains present at the interface, namely with Tyr22, Tyr53, Tyr129 and Tyr152 The only exception was...

Ngày tải lên: 18/02/2014, 17:20

... CTXS-Xyl2-Me complex were not detected within [S-Xyl2-Me]initial ẳ 5200 lM) Protein Inhibitor Ka ã 10)3 (M)1) TRX I S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me ... calorimetry of both domains decreased in the series b-d-xylohexaose (Xyl6) > b-d-xylopentaose (Xyl5) > b-d-xylotetraose (Xyl4) > b-d-xylotriose (Xyl3), with no detectable afnity for b-d-xylobiose (Xyl2), ... (Da)a TRX I (none) None S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me None S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me None S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me 19046.920 n.b 19508.080 19656.081 19804.091...

Ngày tải lên: 07/03/2014, 17:20

... Carrondo MA (2003) Crystallization and preliminary X- ray characterization of cytochrome c¢ from the obligate methylotroph Methylophilus methylotrophus Acta Crystallogr Sect D: Biol Crystallogr 59, 580–583 ... identical geometry and coordi2445 X- ray and unfolding studies of cyt c-550 J A R Worrall et al Table Hydrogen bonding interactions involving buried water molecules (w) and the carboxylates of the ... relaxation times derived from the heme-methyl peaks (180 vs 95 ms for the M100K and wt proteins, respectively) 2447 X- ray and unfolding studies of cyt c-550 J A R Worrall et al Table Thermodynamic...

Ngày tải lên: 07/03/2014, 17:20

... additional hydroxyl group However, the crystal structure of the Phe35fiTyr mutant shows that the ˚ hydroxyl oxygen atom of the side chain of Tyr35 is 3.21 A away from the atom CHB of the pyrrole group ... Z .X. , Xie, Y & Xiao, G.T (1997) Importance of a conserved phenylananine-35 of cytochrome b5 to the protein’s stability and redox potential Protein Eng 10, 578–581 Yao, P., Wang, Y. -H., Xie, Y ... Sun, Y. L., Wang, Y. H., Yan, M.M., Sun, B .Y. , Xie, Y & Huang, Z .X (1999) Structure, interaction and electron transfer between 19 20 21 22 23 24 cytochrome b5, its E44A and/ or E56A mutants and cytochrome...

Ngày tải lên: 08/03/2014, 10:20

... genes were expressed heterologously in E coli XL1 Blue MFR From 500 mL cell culture, approximately 10 mg highly puried MtaA was obtained For X- ray absorption spectroscopy (XAS) the enzyme was equilibrated ... coordinated by sulfur ligand and (N/O) ligands, its total coordination number is In both mutants, zinc is quite differently coordinated, namely by (N/O) and S ligands in His237 Ala, and by (N/O) ligands ... assay conditions The enzyme was studied in the absence and presence of coenzyme M (apparent Km ẳ 40 lM) by XAS at the zinc K-edge EXAFS and XANES spectra are shown in Figs and 2, respectively The...

Ngày tải lên: 17/03/2014, 23:20

... lysozyme with house y digestive and other nondigestive lysozymes In this study, we obtained recombinant bovine stomach lysozyme (BSL2), the most highly expressed lysozyme in the cow stomach X- ray ... surface of the house y lysozyme, and the electrostatic repulsion will be smaller The house y lysozyme may have achieved structural stability by decreasing the positively charged residues The ... the a-helix [30] BSL2 and house y lysozyme lack this proline or glycine residue, and thus the C-helices are longer and the following loops are shorter than those of HEWL and human lysozyme This...

Ngày tải lên: 23/03/2014, 04:21

... 28% and 15% overall yield, respectively X- ray crystallography Recombinant T brucei enolase was expressed and purified as previously described [2] Brie y, bacterial cells (E coli BL21(DE3)pLysS ... Diethylphosphonoacetic acid and the diethyl-2-fluoro-phosphonoacetic acid were linked to O-benzylhydroylamine in Structural flexibility in T brucei enolase the presence of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide ... of ligand (PEP, PAH or FPAH) for and flash-cooled X- ray diffraction data were collected from two native crystals (referred to as sulfate_2 and sulfate_3 in Table 1) and four ligand-cocrystallised...

Ngày tải lên: 23/03/2014, 07:20

... amides of Asn85 and Lys64 The C3-hydroxyl group forms extensive hydrogen bonds with the side chains of Lys64, Asp100, Thr60 and Gln239 The absolutely conserved residues Lys64 and Asp100 are also ... The C5-hydroxyl group of shikimate forms hydrogen bonds with the side-chain amides of Asn58 and Asn85 The C4-hydroxyl group of shikimate interacts with the carboxylate group of Asp100 and with ... Gln239 and the two catalytic active residues Lys64 and Asp100 The carboxyl-binding residues Ser13, Ser15 and Tyr211 are conserved in both AroE and YdiB, but not in SDHL The most noteworthy residue...

Ngày tải lên: 30/03/2014, 02:20

... 164 x, y, z x +1 ,y, z x +1 ,y, z 1 -x, y +1 ⁄ 2,-z x, y, z x, y, z x, y, z x, y, z x- 1 ,y, z 1 -x, y- 1 ⁄ 2,1-z x, y, z x, y +1,z x, y +1,z x, y- 1,z x, y- 1,z x, y, z 1 -x, y- 1 ⁄ 2,1-z x, y, z 1 -x, y +1 ⁄ 2,-z x, y +1,z x, y, z x, y- 1,z ... x, y- 1,z x, y, z x, y- 1,z 3.024(4) 2.783(5) 3.016(4) 3.138(5) 3.021(5) 2.776(5) 3.273(5) 3.122(5) 2.814(4) 2.848(5) 3.221(4) 2.767(5) 3.083(5) x, y, z x, y, z 1 -x, y- 1 ⁄ 2,-z 1 -x, y- 1 ⁄ 2,-z x, y, z x, y, z x, y, z ... 2,-z x, y, z x, y, z x, y, z x, y- 1,z x, y, z x- 1 ,y, z x- 1 ,y, z x- 1 ,y, z x, y, z FEBS Journal 272 (2005) 5079–5097 ª 2005 FEBS Y In et al Conformational comparison of endomorphin-2 and its C-terminal free...

Ngày tải lên: 30/03/2014, 20:20

... À x Š; x x x2 ð74Þ F1 x; ZÞ ¼ f13 x; ZÞ ¼ f12 x2 ; ZÞ exp½Àb3 x2 À x Š; x x x3 > h ib4 > > : f14 x; ZÞ ¼ f13 x3 ; ZÞ x ; x x x4 x3 where < f21 x; ZÞ ¼ a expðÀb1 x þ ðZ À aÞ expðÀcxÞ; x ... (1996): X > < s1 x; ZÞ ¼ di ½exp x À 1Ši ; x xi S x; ZÞ ¼ i¼1 > : s2 x; ZÞ ¼ ½Z À s1 x; ZÞ À t2 Šg1 x þ t2 g2 x þ s1 x1 ; ZÞ x1 < x ð66Þ where g1 x ¼ À exp½t1 x1 À x Š and g2 x ¼ À exp½t3 ... expðÀcxÞ; x x1 x x x2 F2 x; ZÞ ¼ f22 x; ZÞ ¼ f21 x1 ; ZÞ exp½Àb2 x1 À x Š; : f23 x; ZÞ ¼ f22 x2 ; ZÞ exp½Àb3 x2 À x Š; x x x3 Z ð75Þ where Z 100 The parameters for the calculation of F (x, Z ),...

Ngày tải lên: 10/04/2014, 18:53

Ngày tải lên: 13/05/2014, 09:20

... Fragment Screening Using X- Ray Crystallography 39 a X X NH X = C,N,O X = C,N O O O O N H O X Y Y X N N NH C=O C O C C NH Y X b C=O NH C C O NH C X Y Y N X H N H N O N Pyrrole Imidazole Furan Benzene ... 36–45], and are also covered in more depth by Wyss and coworkers [46] 2.2.2 X- Ray Crystallography X- ray crystallography is covered in detail by Bauman et al [47], Davies and Tickle [48], and Hennig ... Brough, Andrew Massey, and Roderick E Hubbard Combining NMR and X- ray Crystallography in Fragment-Based Drug Discovery: Discovery of Highly Potent and Selective BACE-1 Inhibitors Daniel F Wyss, Yu-Sen...

Ngày tải lên: 29/05/2014, 23:54

... Mention X- ray astronomy” to people and too often they think we send beams of X rays into space to obtain an X ray of an object Many of us who carry out research on X- ray energies quickly learn ... project The X- ray and gamma -ray bands are two such areas  The Restless Universe Nearly everyone is familiar with the X- rays used in dentists’ and doctors’ offices There, the X rays have interacted ... s, X rays were believed to exist only in the laboratory because of the difficulty of creating them Laboratory experiments established that air absorbs X rays, explaining the lack of X- ray detections...

Ngày tải lên: 11/06/2014, 10:39