visualization of mouse embryo angiogenesis by fluorescence based staining

... maternal to embryonic control of development can be expressed by the pattern of protein synthesis based on the quantitative histone analysis7 However, these studies on embryonic development based ... considered that the possibility of practical application of Raman spectrum can be proved Materials and Methods Mouse embryo In this study, 5 stages of mouse eggs and embryos were used; unfertilized, ... development of mouse embryo and its quality based on molecular information, obtained nondestructively using Raman spectroscopy The detailed analysis of Raman spectra measured in situ during embryonic

Ngày tải lên: 04/12/2022, 15:32

... 4, 5) These data showed that the transcriptional profiles of CECs in SSc were profoundly different from the tran-scriptional profiles of CECs of healthy donors, indicating that the two populations ... of the cycling point where PCR product is detectable by means of fluorescence emission (Threshold cycle or Ct value) As previously reported, the Ct value correlates to the starting quantity of ... 0.0001% of mononuclear cells [11,29] Flow-cytometry offers the advantage of a rapid and accessible technique [29,30], with the avail-ability of multiple markers as well as the possibility of distinguishing

Ngày tải lên: 12/08/2014, 14:22

... variation in a large set of metabolite profiles. In comparison to other methods our visualization approach facilitates the identification of interesting groups of metabolites by means of a convenient ... intensity-based clustering. In this plot, the distribution of marker candidates of a particular Visualization of one-dimensional self-organizing map after clusteringFigure 2 Visualization of one-dimensional ... of the... Scatter plot of sample-based PCA loadings Figure 12 Scatter plot of sample-based PCA loadings Visualization of PCA loadings for all marker candidates of the experiment Loadings

Ngày tải lên: 12/08/2014, 17:20

... University of Delhi) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMISTRY NATIONAL UNIVERSITY OF SINGAPORE 2010 Trang 2Characterization of Diffusion Behavior of a ... curves of SBD-TMR versus other raft and nonraft Figure 3.2: The G(τ) graph of auto-fluorescence of SHSY5Y and autocorrelation curve of SBD-TMR in solution 58 Figure 3.3: The distributions of diffusion ... emerging field 6 1.2.5.1 Role of lipid rafts in signal transduction pathways 16 1.2.5.2 Role of lipid rafts as platforms for entry of pathogens 18 2.2.1 Principle and theory of fluorescence correlation

Ngày tải lên: 12/09/2015, 09:55

... several applications of FCS in living zebrafish embryos, showing that single molecule‐based studies in living organism are possible:  1 The  autofluorescence  expression  pattern  of  zebrafish  embryo  was  studied first to minimize background interference. The autofluorescence distribution was examined in the embryo body, and the autofluorescence spectrum and intensity was investigated.  ... FOR THE DEGREE OF DOCTOR OF PHILOSOPHY  DEARTMENT OF CHEMISTRY  NATIONAL UNIVERSITY OF SINGAPORE  2009   Trang 2This  work  is  a  result  of  collaboration  between  the  Biophysical  Fluorescence Laboratory at Department of Chemistry, National University of Singapore (NUS) and the Fish Development Biology Laboratory at Institute of Molecular and Cell Biology (IMCB), under the supervision of Associate Professor Thorsten Wohland (NUS) and Associate Professor Vladimir Korzh (IMCB), between July 2004 and November 2008.  Pan,  ... applicability  of  FCS  to  study  molecular  processes  in  embryo  was demonstrated  by  the  determination  of  blood  flow  velocities  with  high  spatial resolution  and  the  determination  of  diffusion 

Ngày tải lên: 14/09/2015, 08:41

... 1INVESTIGATION OF MICROFLUIDICS INCHANNELS AND TISSUES BY FLUORESCENCE CORRELATION SPECTROSCOPY (FCS) PAN XIAOTAO (B.Eng., USTC, China) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... µm by extracting photon counting data in the middle portion of line scans The flow angle was calculatedwith the known parameters of flow speed, line scan speed and netspeed A proof of concept of ... cross section of molecules τ Variable of time shift θ Flow angle in XY plane V Microfluidic flow speed Trang 15Chapter 1Introduction The studies of liquid fluid, one of the phases of matter in

Ngày tải lên: 14/09/2015, 14:13

... LIU PING NATIONAL UNIVERSITY OF SINGAPORE 2007 Trang 2THE STUDY OF INTERACTIONS OF TRANSMEMBRANE RECEPTORS AND INTRACELLULAR SIGNALING PROTEINS IN LIVE CELLS BY FLUORESCENCE CORRELATION AND ... pursuing for MSc and PhD Trang 4Table of Contents Acknowledgements i Table of contents ii Summary v List of Tables viii List of Figures ix List of Symbols xi Chapter 1 Introduction ... quantification of complex (dimer) percentages of interacting proteins and the determination of the equilibrium dissociation Trang 8constants of binding proteins The experimental setups of FCS/SW-FCCS

Ngày tải lên: 14/09/2015, 14:25

... (FL) [82] 2) by recruitment of DC in situ by Flt-3L or chemokines/chemokine receptors 3) by enhancing vaccine capturing and processing by DC 4) by promoting DC maturation and 5) by targeting ... properties of DC, vaccine efficacy could be improved, perhaps, by altering DC functions in several ways: 1) by in vivo increasing of DC number by stimulating DC replication or survival signal by Flt-3 ... Trang 1INDUCTION OF ANTI-TUMOR RESPONSE BY DENDRITIC CELL-BASED VACCINATION FONG CHOY LEN B.Sc (Hons) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MICROBIOLOGY NATIONAL

Ngày tải lên: 16/09/2015, 15:54

... reduces the fluorescence from the reporter (R) fluorophore by means of fluorescence resonance energy transfer (FRET) This is the inhibition of one dye caused by another without emission of a proton ... overlap of TSAB and TSBAB binding sites [11] Figure 4 Schematic representation of different forms of the TSHR (i) Intramolecular cleavage of the single polypeptide chain is followed by removal of ... (90o light scatter), as well as for presence of colored fluorescence Thus by measuring the fluorescence intensity of each cell after interrogation by a laser beam, the machine is able to distinguish

Ngày tải lên: 07/10/2015, 09:55

... followed by those prepared using EDC/NHS-activated antibodies (9%), and finally, by those prepared by direct adsorption (7.5%), as shown in Fig 6D The stability of the immunosensors prepared by using ... value of Ret After detection of cells of V cholerae O1, the immunosensor was dipped into a Fig 7 (Color online) (a) Stability of the immunosensor at different times for 135 day, (b) specificity of ... effects of differ-ent antibody immobilization strategies on the response of CeO2-NWs-based immunosensors We determined the best immobilization method to apply for the design of CeO2-NWs-based immunosensors

Ngày tải lên: 01/09/2017, 12:49

... determine roof branches and constitute the base shape for roof creation Thepresented results demonstrate a set of buildings automatically generated, each oneholding a set of gable roofs unified by branches ... produced by PG3D [ 5 ] An available set of information was considered to generate the virtual model of this urban area, that has considerable degree of resemblance 2.2.11 Ontology-Based Generation of ... Modelling of Virtual Traversable Buildings 252.3.4 Lazy Production of Virtual Building Interiors The concept of real-time generation of interior divisions was exploited by Hahn et al [6] The plans of

Ngày tải lên: 14/05/2018, 15:12

... number of effective tillers in PBW 550 Trang 6Table.1 Effect of date of sowing and varieties on plant height and yield attributes of wheat Date of Sowing Genotypes Table.2 Effect of date of sowing ... the years of study using the seed rate of 100 kg/ha at a row to row spacing of 20 cm Full dose of P2O5 (62.5 kg/ha) and 1/3rd dose of nitrogen (50 kg/ha) was applied basal at the time of sowing ... Grains/earhead Pooled means of two years indicate that numbers of grains/spike were not affected by time of sowing Wheat cultivars also did not affect number of grains per spike significantly

Ngày tải lên: 15/05/2020, 10:22

... bean wave-length of 349 nm, power of 53 μJ, aperture of 46, speed of 2, specimen balance of 41, head current of 100 %, plus fre-quency of 4046 Hz), before collecting it by a cap of 500 μL micro-centrifuge ... at 0.96 mg/mL of Sa B, 0.992 mg/mL of DHTan I, 0.954 mg/mL of Tan I, 0.991 mg/mL of CTan, 1.028  mg/mL of Tan IIA The series concentrations of mixed working solution were prepared by diluting the ... ARTICLETissues-based chemical profiling and semi-quantitative analysis of bioactive components in the root of Salvia miltiorrhiza Bunge by using laser microdissection system combined with UPLC-q-TOF-MS

Ngày tải lên: 29/05/2020, 14:22

... risk of neoplasia com-pared to single infections [8, 13, 14] HPV viral load, as a product of the number of infected cells and the number of virus per infected cell, is there-fore influenced by ... was approved by the ethics commit-tee of Affiliated Shengjing Hospital of China Medical University Specimens were obtained from patients in the Department of Obstetrics and Gynecology of the hospital, ... cases (6 cases of single infec-tion and 14 of multiple infecinfec-tions) were detected by the sequencing but not by the BMRT HPV PCR assay In contrast, the other 56 cases (32 cases of single infection

Ngày tải lên: 22/09/2020, 23:31

... based on their frequency of occurrence (i.e increased success rate of experiment) together with the short life-span of the dko mouse (~10-12 weeks) When we normalize both studies to the dko mouse ... improvement of contractile function The underlying cause of weakened contractile performance of the end-stage heart failing myocardium is often independent of the originating cause of heart failure ... et al.: Improvement of cardiac contractile function by peptide -based inhibition of NF-B in the utrophin/ dystrophin-deficient murine model of muscular dystrophy Journal of Translational Medicine...

Ngày tải lên: 18/06/2014, 19:20

... IMPROVEMENT OF MOUSE EMBRYO PROGENITOR-DERIVED INSULIN-PRODUCING CELLS FOR TYPE DIABETES THERAPY SHAO SHIYING (M.Sc., TONGJI University) A THESIS SUBMITTED FOR THE DEGREE OF PHILOSOPHY OF DOCTORATE ... Kiang Lim, GuoDong Li (2011) Correction of hyperglycemia in type diabetic models by transplantation of encapsulated insulin-producing cells derived from mouse embryo progenitor J Endocrinol 208:245-255, ... Luo, Wataru Nishimura, Arun Sharma, GuoDong Li Improvement of insulin-producing cells derived from mouse embryo progenitor by enhancement of MafA expression Manuscript to be submitted Conference...

Ngày tải lên: 11/09/2015, 09:58

... provides further guidelines for the rational design of antimicrobial peptides.      VIII        Investigation of Peptide‐Lipid Interaction by Fluorescence Correlation Spectroscopy    List of Figures  Fig 1.1 Schematic representation of the Gram‐negative bacteria cell wall.  ... Fig 4.15 TIRF image of uneven distribution of V4.  126    X    Investigation of Peptide‐Lipid Interaction by Fluorescence Correlation Spectroscopy        List of Tables  Table 1.1 Clinical developments of cationic antimicrobial peptides.  ... Investigation of Peptide‐Lipid Interaction by Fluorescence Correlation Spectroscopy        Other  promising  sources  of AMPs  are  synthetic  peptides.  A  series  of AMPs  have  been synthesized by modifying the sequence of their natural analogues, or according to ...

Ngày tải lên: 11/09/2015, 10:05

... DETECTION OF BIOLOGICALLY RELEVANT ANIONS BY FLUORESCENCE AND NIR MOLECULAR PROBES QUEK YI LING (B Sc.(Hons.), National University of Singapore) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... measurement of HE oxidation by myricetin .35 2.2.8 pH dependency of HE oxidation by myricetin 36 2.2.9 Myricetin decomposition in the presence and absence of HE .36 2.2.10 Determination of acid ... mechanism of the reaction of luminol with O2●− Figure 1.7 CL mechanism of the reaction of lucigenin with O2●− Figure 2.1 Chemical structure of the flavonols used Figure 2.2 HPLC chromatograms of E+,...

Ngày tải lên: 30/09/2015, 06:14

... number of copies of GAL4 driver and reporter, embryo aging temperature, and the removal of autofluorescence interference were also described Chapter presents the results and discussion of FCS ... good proof of principle experiments for us We also compared in situ FCS measurements from the top (nearer to ventral side of the embryo) and bottom (nearer to the dorsal of the embryo) embryonic ... α i Fi   i  (2.22) where α i is the ratio of fluorescence yield of particle i to the fluorescence yield of particle 1, Fi is the mole fraction of species i in the sample and g3 Di = g xi g...

Ngày tải lên: 12/10/2015, 17:36