viral vectors for veterinary vaccines

... systems for vector development and gene transfer Chapter contributions provide perspective in the use of viral vectors for applications in the brain and in the central nervous system Viral Vectors for ... Virus Type 1-Based Vectors for Gene Delivery to Human Hematopoietic Stem Cells Ali Ramezani and Robert G Hawley 467 Semliki Forest Viral Vectors for Gene Transfer Jarmo Wahlfors and Richard ... and use of viral vectors for gene transfer and gene therapy I would like to thank the many chapter authors for their contributions They are all experts in various aspects of viral vectors, and...

Ngày tải lên: 11/04/2014, 10:39

... 2003) 1.4.2 Viral vectors Viral vectors are the most efficient vectors to transduce mammalian cells, and therefore are employed in most gene delivery experiments A variety of viral vectors, including ... used for gene therapy applications, and were rarely used for 12 Chapter Introduction gene delivery to the CNS because of their poor in vivo performance Non -viral and viral vectors are therefore ... regulating the gene expression 11 1.4 Vectors used for gene delivery to the CNS 12 1.4.1 Non -viral vectors 13 1.4.2 Viral vectors 14 1.4.3 Hybrid viral vectors 17 1.5 Objectives of this study 19 V...

Ngày tải lên: 16/09/2015, 08:30

... production 11 Table 1.1 Vectors and delivery systems for gene therapy Vectors Non -viral vectors Retrovirus Advantages • easy to produce • stable gene expression due to viral genome integration ... 3.3.9 Packaging of amplicon viral vectors 3.3.10 Analysis of cell cycle-dependent transgene expression in pC8-36 amplicon viral vectors 3.3.11 Effect of transduction of viral vector on the cell ... Binding buffer for isolation of nuclear extract for Electromobility shift assay (EMSA) (Wu et al., 2001) 2.1.43 Buffer A for isolation of nuclear extract (Wu et al., 2001) 2.1.44 Buffer C for isolation...

Ngày tải lên: 16/09/2015, 15:54

... [13] This review briefly describes the viral vectors, including the replication defective vectors, the targeted vectors and replication-competent oncolytic vectors, and their use in cancer gene ... "Trojan horse" vectors for cancer therapy, the mechanisms involved, and the foreseeable promises and problems when compared with existing viral vector systems The development and use of viral vector ... [33] (G) Viral vector-associated multifunctional particles (MFPs) Recently, a concept of multifunctional particles (MFPs) based viral vectors has emerged The idea incorporated viral vectors' ...

Ngày tải lên: 14/08/2014, 19:22

... Clinicopathologic principles for veterinary medicine Clinicopathologic principles for veterinary medicine Edited by WAYNE F ROBINSON and CLIVE R R HUXTABLE School of Veterinary Studies, Murdoch ... include red cells for gas transport, neutrophils and macrophages for phagocytosis, platelets for hemostasis, lymphocytes for antibody production and eosinophils and basophils for involvement in ... members of the School of Veterinary Studies, Murdoch University, Murdoch WA 6155, Australia Preface This book is written for veterinary medical students as a primer for their clinical years...

Ngày tải lên: 17/02/2014, 14:20

... also show more genre and content effects For example, the sentiment enriched vectors for ghastly are truly semantic alternatives to that word, whereas the vectors without sentiment also contain ... section for inducing word representations from the topic matrix To train the 50-topic LDA model we use code released by Blei et al (2003) We use the same 5,000 term vocabulary for LDA as is used for ... clearly outperform those of other VSMs, and perform best when combined with the original bag of words representation The variant of our model trained with additional unlabeled data performed best,...

Ngày tải lên: 20/02/2014, 04:20

... Banno for the PLD2 antibody, Dr Greg Hannon for the pSM2 vector, Dr Roger Y Tsien for the pRSET-B-mCherry, and Dr Jen-Chih Hsieh for the pRK-human IgG construct We also thank Dr Jian Cao for allowing ... codex.cshl.edu (for miR30 system), and the published guidelines for selecting highly effective siRNA sequences [20] The oilgos for candidate sequences also contained cohesive ends for our simplified ... oligos including cohesive ends and a specific sequence for each artificial miRNA (64 nucleotides for the miR155based system and 67 nucleotides for the miR30-based system) were annealed, and cloned...

Ngày tải lên: 07/03/2014, 11:20

Ngày tải lên: 15/03/2014, 17:20

Ngày tải lên: 22/03/2014, 22:20

... USA 9600 Garsington Road, Oxford, OX4 2DQ, United Kingdom For details of our global editorial offices, for customer services and for information about how to apply for permission to reuse the copyright ... Register of Veterinary Nurses 2008 Veterinary Nurses and the Veterinary Surgeons Act 1966, pp 239–242 Royal College of Veterinary Surgeons (2007) Guide to Professional Conduct for Veterinary Nurses ... consequence of their own actions but also for the injurious actions of an employee, including veterinary nurses and student veterinary nurses Therefore, veterinary nurses are not entitled to undertake...

Ngày tải lên: 29/03/2014, 11:21

... t a g e s of Viral Vectors for Vaccine Delivery C o n s t r u c t i o n of Safer Viral Vectors for Vaccine D e l i v e r y E x a m p l e s of R e p o r t e d Viral V e t ... 135 136 138 141 141 Viral Vectors for Veterinary Vaccines MICHAEL I II III IV V VI VII SHEPPARD Introduction and Background Viral Vector C o n s t r u c t i o n ... possible the development of vaccines for diseases for which there are no conventional vaccines and of replacement of conventional vaccines with safer and/or more effective vaccines as needed New and...

Ngày tải lên: 29/03/2014, 15:20

... refs 8–10 for typical results) The protocol can be used for all adherent cell types For suspension type cells, cells need to be spun down before changing the medium at a speed suitable for the ... tetrazolium salt, XTT, to form an orange formazan dye by dehydrogenase activity in active mitochondria Therefore, this conversion only occurs in living cells The formazan dye formed is soluble in ... of vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers Hum Gene Ther 7, 2123–2133 11 Kabanov, A V and Kabanov, V A (1995) DNA complexes with polycations for...

Ngày tải lên: 11/04/2014, 09:59

... visualization of viral infection in mosquito vectors [9,10] Co-transfecting replicon containing cells with a plasmid that expresses the viral structural proteins can produce pseudo-infectious viral particles ... Sindbis virus RNA vectors for heterologous gene expression Proc Natl Acad Sci U S A 1998, 95:12989-12994 Zhang J, Frolov I, Russell SJ: Gene therapy for malignant glioma using Sindbis vectors expressing ... expression is therefore a valuable tool for the functional evaluation of gene function in insects Further evaluation of the utility of antisense expressing SINV replicons needs to be performed to determine...

Ngày tải lên: 18/06/2014, 18:20

... removed, processed for viral titration or sectioned on cryostat (Jung Frigocut; 14 µm thick sections) Sections were fixed with 3.7% formaldehyde or acetone for 30 and processed for indirect immunofluorescence ... post-infection, cell culture supernatants were processed for viral titration; cells were fixed and processed for immunofluorescence detection for viral antigens (see above) or neural cell typing, using ... immunoreactive cells expressed viral antigens (data not shown) Although our study was limited in its scope, the results indicate that WNV strain IS-98-ST1 is suitable as viral model for West Nile encephalitis...

Ngày tải lên: 18/06/2014, 22:20

... quantization of beamforming vectors in multiuser MIMO beamforming systems We discussed several criteria for designing local codebooks and presented a number of constructions for two and four transmit ... radius for a nonlocal codebook can also be defined, which we call c(F ) The main difference between the covering radius for a nonlocal and local codebook is that the latter is only computed for vectors ... following form: ⎡ wk = ⎣ ⎤ − γ0 ⎦ γ0 wk , (9) where wk is a Nt − × unit norm vector We now summarize some general principles for constructing a ring codebook 5.1.1 Uniform Phase Ring for Nt =...

Ngày tải lên: 21/06/2014, 20:20

... generating function for LP can be written as a rational function: LP (m)tm = EhrP (t) := m≥0 δ0 + δ1 t + + δk(n+1)−1 tk(n+1)−1 , (1 − tk )n+1 whose coefficients δi are non-negative For an elementary ... ≤ for all v ∈ P } If the origin lies in the interior of P then P ∨ is a rational polytope containing the origin, and P = (P ∨ )∨ We restrict our attention to those P containing the origin for ... LP (m) = LP,r (l) The generating function for each LP,r is given by: δ0,r + δ1,r t + + δn,r tn , (2.1) EhrP,r (t) := LP,r (l)tl = (1 − t)n+1 l≥0 for some δi,r ∈ Z Theorem 2.1 Let P be a rational...

Ngày tải lên: 07/08/2014, 15:22

... Engineering of a Viral Cage for Constrained Nanomaterials Synthesis Adv Mater 2002, 14:415-418 Gillitzer E, Willits D, Young M and Douglas T: Chemical modification of a viral cage for multivalent ... The introduction of an accessible cysteine on the surface of the MS2 capsid therefore should create the opportunity for multivalent display of a large number of different potential ligands on its ... structure Based on their accessibility on the surface of the viral capsid, five different amino acids of MS2 coat protein were selected initially for cysteine substitution (Figure 1) Three of the five...

Ngày tải lên: 11/08/2014, 00:22

... of 50 μl Each PCR was cycled at 1×: 95°C for min., 35×: 95°C for 20 sec | 66°C for 20 sec | 72°C for 0.5 - (depending upon template length), and 1× 72°C for Samples were electrophoresed on 1% TAE ... populations for 2.2, 5.3 and 6.3, but only 83 populations for 3.2, and 48 for 4.3 Approximately 10 - 12 weeks after transduction the populations were selected and sufficiently expanded to be harvested for ... formation between days - 11 Viral titer was calculated using the Reed-Muench method for estimating 50% endpoints [57] HIV-1 challenge assay The CEMT4 cell lines with stably integrated shRNA vectors...

Ngày tải lên: 12/08/2014, 04:20