... amount of RXR pulled down by EcR in the presence of ligand was maximum in the case of HsRXR and minimum in the case of the Hs–LmRXR chimera, and LmRXR was between these two (Fig. 9). These data ... concentration of RG-102240 (Fig. 7). These data confirmed the results that the region of LmRXR containing helix 9 and the two loops on either side of helix 9 is responsible for improved per- formance of ... been used in the place of USP in all EcR gene switches developed to date. One of the major limitations of cur- rent versions of EcR gene switches is the requirement of micromolar concentrations of ligand
Ngày tải lên: 07/03/2014, 12:20
... characteristics of two Fpo mutants (DfpoF and DfpoA-O) and the effects of these deletions on the process of energy con-servation Furthermore, it is shown that the protein FpoF functions as an input module of ... lysates of the DfpoA-O mutant or the DfpoF mutant was in the range of 5 mUÆmg)1 of protein and hence 10-fold higher than the rate of H2 production from F420H2 in the absence of CoM-S-S-CoB The H2 ... warranted Dual function of the FpoF subunit of the Fpo complex The cluster encoding the Fpo of Ms mazei and related Methanosarcinastrains is composed of 12 genes (fpoA– O) [3] Analysis of the Fpo subunits
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Protein engineering of pyruvate carboxylase Investigation on the function of acetyl-CoA and the quaternary structure doc
... regulator in the interaction between the active site of the BC domain and the biotin moiety of the BCCP domain Based on these arguments, it is tempting to propose the following hypothesis: in the absence ... purification of the engineered proteins of PC The boundary of the BC and CT domains of PC was estimated as follows: the BC subunit of E coli acetyl-CoA carboxylase is catalytically active and the three-dimensional ... addition of acetyl-CoA and the activity in the absence of acetyl-acetyl-CoA was approximately 0.3% of the maximum (Table 1) PC is known to catalyze the cleavage of ATP in the absence of pyruvate
Ngày tải lên: 16/03/2014, 16:20
Báo cáo Y học: The function of methyl-menaquinone-6 and polysul®de reductase membrane anchor (PsrC) in polysul®de respiration ofWolinella succinogenes doc
... reductaseÕ NrfA of E coli is known to be the catalytic subunit of this enzyme [37] The gene product of nrfD was proposed to encode the membrane anchor of the enzyme and to carry the site of quinol ... are tyrosines, the phenolic hydroxyl groups of which appear to be essential for polysul®de respiration The function of these residues may be explained on the basis of the hypothetical mechanism ... dehydrohydro-genase [3,6±10] The catalytic subunits of the three enzymes are oriented to the periplasmic side of the membrane [4,6,10,11] The three enzymes were isolated from the membrane fraction of W succinogenes
Ngày tải lên: 24/03/2014, 03:21
Chapter 2Communicating Over the Network Quangkien@gmail.com.OverviewDescribe the structure of a network, including the devices and media that are necessary for successful communications. Explain the function of protocols in network communications. Ex potx
... software implementation of a computer networking protocol suite The terms are often used interchangeably Strictly speaking, the suite is the definition of the protocols, and the stack is the ... communications. Explain the advantages of using a layered model to Explain the advantages of using a layered model to describe network functionality. Describe the role of each layer in two ... Over the Network Quangkien@gmail.com Trang 2 Describe the structure of a network, including the devices and media that are necessary for successful communications. Explain the function of
Ngày tải lên: 01/04/2014, 12:20
Báo cáo y học: "Structural insights into the function of the core-circadian factor TIMING OF CAB2 EXPRESSION 1 (TOC1)" ppt
... Trang 7of the middle third is the most distinct (Figure 2) We note that this is true for the other PRR proteins as well [54] The lack of a consensus structure within the middle third of the polypeptide ... origin of func-tion for the amino-terminal module of TOC1 This further supports the phylogeny relations of the amino-terminal module of PRR to genuine RRs [40] In each of the four TOC1 modules, ... an axis of the ROOT MEAN SQUARE DEVIA-TION (RMSD) of the resultant structures [36] From there, each of the related four proteins of each module was proc-essed on SPATIAL ARRANGEMENT OF BACKBONE
Ngày tải lên: 10/08/2014, 09:20
Báo cáo y học: " Analysing the eosinophil cationic protein - a clue to the function of the eosinophil granulocyte" ppt
... foundation for further speculations of the role of ECP, and ultimately the role of the eosinophil. Discovery of the eosinophils Eosinophils were discovered in the blood of humans, frogs, dogs and rabbits ... together with the cysteines are present in all members of the pancreatic ribonuclease family [20]. Analysis of the crystal structure of ECP verified this relationship to these other members of ... role. The physiological function of the granule contained cytotoxic ribonuclease Eosinophils contai n a large amou nt of ECP but the ques- tion is why? What is the function of this protein? There
Ngày tải lên: 12/08/2014, 13:22
Báo cáo y học: " The function of 7D-cadherins: a mathematical model predicts physiological importance for water transport through simple epithelia" pdf
... flux The change of the electrolyte concentration in the LIC, according to the law of mass conservation, equals the sum of the inward and outward electrolyte fluxes divided by the volume of the ... proteins of both cell membranes with the extracellular domains joining directly [2] The major types of these proteins are the claudins and the occludins The TJ are responsible for the sealing of the ... diffusion out of the cleft into the interstitium with D being the over all diffusion coefficient of the electrolyte The second term describes the above mentioned convective flow of ions due to the water
Ngày tải lên: 13/08/2014, 16:20
The Function of PsbS Protein in Plant Photosynthesis Regulation
... for their activation of ~6.0 [98], bind protons, together they would trigger the aggregation of LHCII increasing the hydrophobicity of the environment of the qE-active residues and shifting the ... that the function of PsbS in qE activation is dominant compared to that one of zeaxanthin in the presence of ∆pH Indeed, the absence of both PsbS and zeaxanthin show the same qE reduction as in the ... is not due to the presence of the quencher site in this subunit, but it is due the particular organisation of the complexes in the membranes and the reduced capacity of electron transport and
Ngày tải lên: 24/06/2015, 08:18
Characterization of the function of tight junction proteins in transgenic mice
... available for the transepithelial transport of molecules The first one is the transcellular pathway that requires the solutes to be internalized to cross the epithelial cells The second one is the paracellular ... synchronized contraction of cardiomyocytes The interaction between ZO-1 and Cx43 occurs via the first PDZ domain of ZO-1 and the C-terminus of Cx43 Overexpression of the N-terminal domain of ZO-1 in Cx43-expression ... expressed in the early stage of embryo development, but their function during the different stages of development is not clear Furthermore, ZO proteins are widely expressed in the tissues of adult
Ngày tải lên: 11/09/2015, 16:04
The function of TOM1 l1 in bridging EGFR signaling and endocytosis
... two clusters of lysine residues at each end of the binding domain) on the N terminus of the α subunit and a cluster of conserved lysine residues at the surface of μ2 C-terminal ... THE FUNCTION OF TOM1-L1 IN BRIDGING EGFR SIGNALING AND ENDOCYTOSIS LIU NINGSHENG INSTITUTE OF MOLECULAR AND CELL BIOLOGY NATIONAL UNIVERSITY OF SINGAPORE 2007 THE FUNCTION OF TOM1-L1 ... 2) out of the nine that CALM domain comprise the ANTH contact the membrane The interaction takes place between the solvent exposed sidechains of three consecutive... as the Golgi
Ngày tải lên: 14/09/2015, 08:41
The function of DTRAF1 in drosophila neuroblast asymmetric cell division
... et al., 1996) The boundaries of these two cortical domains abut roughly midway along the AP axis In the absence of any one of the anterior group proteins, the other two members of the complex are ... only one of the two centrosomes is within the reach of their signaling activity and thus adopts different properties from the other centrosome This model raises the question of whether there is ... toward the posterior PAR-1 appears to be downstream of PAR-2, as the polarized distributions of PAR-2 and the anterior complex are not affected by the absence of PAR-1 The other two PAR proteins, the
Ngày tải lên: 15/09/2015, 17:11
The function of anxiety fear related genes in animal models 3
... representation of the rat CCK2 receptor Figure 1-4 Alignment of members of the corticotropin-releasing factor (CRF) peptide family Figure 1-5 Dendrogram showing the relatedness of corticotropin-releasing ... mice in the elevated plus-maze Figure 7-6 The anxiogenic effect of CCK4 in the elevated plus-maze was blocked by CCK2 receptor antagonist CR2945 Figure 7-7 The anxiogenic effect of CCK4 in the ... plus-maze The time spent on the open arms was expressed as percentage of the total session time (300 s) Five rats with the longest open arm times were used as the low anxiety group and 5 rats with the
Ngày tải lên: 17/09/2015, 17:20
The function of anxiety fear related genes in animal models 2
... in the rat forebrain (the central part of the medial preoptic nucleus, the encapsulated part of the Trang 5bed nucleus of the stria terminals, and the posterodorsal part of the media nucleus of ... relative to the 5-HT neurons where they play the role of both 5-HT autoreceptors and 5-HT heteroreceptors The former control the release of 5-HT while the latter control the release of non-serotonin ... 5-HT has been the topic of intense research activity, which has led to the discovery of a range of potential drug targets: the receptors, the metabolising and synthetic enzymes, the uptake sites
Ngày tải lên: 17/09/2015, 17:20
The function of anxiety fear related genes in animal models
... Distribution of CCK receptors 2.2.3 The molecular biology of the CCK1 and CCK2 receptors i iii iv viii x 1 1 1 2 3 3 3 4 5 5 6 8 Trang 6Table of Contents v 2.3 Function of CCK-related ... FEAR CONDITIONING WANG HONG (MMed, BSc) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF PHARMACOLOGY NATIONAL UNIVERSITY OF SINGAPORE 2003 Trang 2Acknowledgements ... discussion throughout the course of this study Associate Professor Wong Tsun Hon, Peter and Associate Professor Tan Chay Hoon, Department of Pharmacology, NUS, are highly appreciated for their invaluable
Ngày tải lên: 17/09/2015, 17:20
Investigate the function of kinectin isoforms in endoplasmic reticulum dynamics
... isoforms in DsRed-ER HeLa cells Fig 2.11 The co-accumulation of the ER and 160 kDa kinectin isoforms of Int4 at the leading edge of migrating cells Fig 3.1 The effect of stable expression of ... differences in their functions in the future iii LIST OF FIGURES Fig 1.1 The structure of kinectin Fig 1.2 Novel kinectin isoforms Fig 1.3 The current model for the maintenance of ER dynamics ... mitosis and the role of microtubules 27 vi 1.6 The purpose and rationale of the thesis work 29 Chapter The subcellular localization of kinectin isoforms in DsRed-ER HeLa 31 2.1 The construction
Ngày tải lên: 08/11/2015, 16:45
Genome-wide identification of mitogen-activated protein kinase gene family in Gossypium raimondii and the function of their corresponding orthologs in tetraploid cultivated cotton
... re-sponses and the regulation of certain components of mul-tiple stress-signaling pathways [25,26] Nevertheless, our knowledge of the MAPK gene family in cotton is limited The completion of the genome-sequencing ... structures further revealed the classification of the GrMAPK family (Figure 3) GrMAPKsin groups A and B exhibit a highly conserved distribution of exons and introns consisting of six exons of conserved ... al [32]), and the scaffolds name from G raimindii genome was showed in the bracket Lines were drawn to connect duplicated genes The nomaclature of MAPKs were based on the order of the chromosomes
Ngày tải lên: 27/05/2020, 00:40
Tài liệu Báo cáo khoa học: Chemical approaches to mapping the function of post-translational modifications ppt
... [11,12]. The study of these events is taxing, as the biosynthesis of glycoproteins is not tem- plate driven. This results in the formation of so-called ‘glycoforms’ [11,12], proteins with the same ... 1957 catalyze enhanced hydrolytic degradation of the target protein. More recently, the glycoMTS method has allowed the synthesis of the first examples of a homogeneous protein bearing symmetrically ... scaf- fold. These terms reflect not only the linearity or convergence of the chemical steps that may lead to a given synthetic protein, but also the structural strategy that links the (linear) segments of...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu KRONE - the effect of Errors in TCP applications performance (full) pdf
... purposes the code field looks like this: The second part of the diagram shows how the code bits would be set when one of the TCP entities wants to indicate the end of the session to the other TCP entity. ... bytes, reflecting the fact that 1460 bytes of data plus the 20 bytes in each of the IP and TCP headers and the 18 bytes of Ethernet header and trailer will total 1518 bytes. This is the maximum size of an Ethernet ... Networks There are three types of error detection schemes that we will study. The first of these is the detection of an individual bit that is corrupted. This is usually the responsibility of the...
Ngày tải lên: 09/12/2013, 16:15
Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx
... posi- tioned in the connecting loops. Approximately one- third of all mutations in the BTK gene are missense and some of these reduce the stability of the protein. This is exemplified by mutations in the ... identify individuals with mild disease, thereby providing the missing data. The phenotype of XLA and the potential of BTK and ITK inhibitors The outcome of defective BTK signaling in humans has ... regarding their potential function, it was the identification of BTK, as the kinase affected in XLA [9,10], which immediately made TFKs known to the wider scientific community. In the same year, the xid...
Ngày tải lên: 14/02/2014, 18:20
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